| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_012967 | 3,483,588 | T→G | L635R (CTG→CGG) | malT → | HTH‑type transcriptional regulator MalT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 3,483,588 | 0 | T | G | 99.0% | 818.7 / ‑3.7 | 191 | L635R (CTG→CGG) | malT | HTH‑type transcriptional regulator MalT |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (76/113); total (78/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01 | |||||||||||
Only 100 of 202 total aligned reads displayed.
TGGCAATGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGA > NC_012967/3483519‑3483656 | ggCAATGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGa < 1:5071642/72‑1 (MQ=255) gCAATGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGaa > 1:3436614/1‑72 (MQ=255) aaTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGaaaa < 2:3559794/72‑1 (MQ=255) aaTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGaaaa > 2:3602593/1‑72 (MQ=255) aaTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGaaaa > 2:2828789/1‑72 (MQ=255) aaTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGaaaa < 1:6642516/72‑1 (MQ=255) aTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAAt > 2:2983181/1‑72 (MQ=255) aTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAAt > 1:1882945/1‑72 (MQ=255) aTGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAAt < 1:6369791/72‑1 (MQ=255) tGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATc < 2:5625322/72‑1 (MQ=255) ttGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTNAACCGTCGGGAAAATctg > 1:2562351/1‑72 (MQ=255) tGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATctgc < 2:5349547/72‑1 (MQ=255) taGTCAATGCTGGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATctgct < 2:3841895/71‑1 (MQ=255) gAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATctgct < 1:5368621/72‑1 (MQ=255) aGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATctgctg < 1:3522426/72‑1 (MQ=255) cAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTgggg < 2:2056258/72‑1 (MQ=255) aaTGCTCGCTGGCCCTTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGa < 2:5765892/72‑1 (MQ=255) aaTGCTCGCTGGCCCGTGGTGATCTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGGAAATCTGCTGGGGa > 1:4088509/1‑72 (MQ=255) tGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGaaa > 1:3620288/1‑71 (MQ=255) tGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAAt < 2:1423206/72‑1 (MQ=255) tGCTCGCTGGCCCGTGGTGATTTAGATAACGCACGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAAt < 1:959016/72‑1 (MQ=255) ggCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAAt < 1:2612984/71‑1 (MQ=255) cTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATgg < 1:6446214/72‑1 (MQ=255) tCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGc > 1:3102210/1‑72 (MQ=255) tCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGc < 2:6539388/72‑1 (MQ=255) tCGCTGGCCCGTGGTGATTTAGATAACGCACGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGc < 1:5255731/72‑1 (MQ=255) gCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCaa < 1:2834306/72‑1 (MQ=255) gCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCaa < 2:5956734/72‑1 (MQ=255) cTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCaaa < 2:5049582/72‑1 (MQ=255) cTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCaaa < 1:989837/72‑1 (MQ=255) cTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCaaa > 1:3738041/1‑72 (MQ=255) ggCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAata > 2:6380857/1‑72 (MQ=255) gCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAatat < 1:4467673/72‑1 (MQ=255) gCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGCGAATGGCAAatat > 2:5150731/1‑72 (MQ=255) cGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATcac < 1:5310218/72‑1 (MQ=255) gtggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATcaca > 1:679600/1‑72 (MQ=255) gtggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATcaca > 1:1975598/1‑72 (MQ=255) tggtgATTTAGATAACGCCCGTAGCTAGCGGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAg < 1:3937907/72‑1 (MQ=255) tggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAg < 2:1880473/72‑1 (MQ=255) tggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAg > 2:5976622/1‑72 (MQ=255) ggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGc > 1:4927707/1‑72 (MQ=255) ggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGc < 1:1720861/72‑1 (MQ=255) ggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGc < 2:1971580/72‑1 (MQ=255) gtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCCCAACg > 1:2438103/1‑72 (MQ=255) gtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCg < 1:6014602/72‑1 (MQ=255) gATTTAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGAc < 2:1213986/72‑1 (MQ=255) tttAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTg < 2:1320915/72‑1 (MQ=255) tttAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTg < 1:226429/72‑1 (MQ=255) tgaGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTgg < 2:3150104/70‑1 (MQ=255) tAGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGa > 2:5031434/1‑72 (MQ=255) aGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAt > 2:6033339/1‑72 (MQ=255) aGATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAt > 1:4561272/1‑72 (MQ=255) gATAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAtc < 2:3310229/72‑1 (MQ=255) caTAACGCCCGTAGCAAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAtc < 2:2439730/71‑1 (MQ=255) aTAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAtct < 1:4813058/72‑1 (MQ=255) tAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAtctc > 2:6443697/1‑72 (MQ=255) tAACGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGAtctc > 1:4832419/1‑72 (MQ=255) aaCGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATctct < 2:5271509/72‑1 (MQ=255) aaCGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATctct > 1:1430329/1‑72 (MQ=255) actcccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATTGCAAATATCACAGCGACTGGATCTCTa < 2:274960/69‑1 (MQ=255) cGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTaa < 1:1142318/72‑1 (MQ=255) cGCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTaa < 2:4960655/72‑1 (MQ=255) gCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAAc < 1:6381085/72‑1 (MQ=255) gCCCGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAAc > 1:518101/1‑72 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg < 1:893932/72‑1 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg > 2:4476102/1‑72 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg > 2:2283230/1‑72 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg < 1:5874533/72‑1 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg > 1:5657304/1‑72 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg < 1:1531835/72‑1 (MQ=255) cccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACg < 2:3530497/72‑1 (MQ=255) ccGTANCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGc < 2:591404/72‑1 (MQ=255) ccGTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGc < 1:1209221/72‑1 (MQ=255) ccGTACCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGc < 2:798574/72‑1 (MQ=255) gTAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCca > 1:5122500/1‑72 (MQ=255) tAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaa > 1:79234/1‑72 (MQ=255) tAGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATACATAACGCcaa > 1:4686139/1‑72 (MQ=255) aGCCAGCTGAACCGTCTGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaac > 1:6142180/1‑72 (MQ=255) aGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaac > 1:2416676/1‑72 (MQ=255) aGCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaac > 1:3206727/1‑72 (MQ=255) gCCAGCTGAACCGTCGGGAAAATCTGCTGGTGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaaca > 1:2071992/1‑72 (MQ=255) gCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaaca < 2:4908870/72‑1 (MQ=255) gCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaaca < 2:1261266/72‑1 (MQ=255) gCCAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCcaaca > 1:5912469/1‑72 (MQ=255) cAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCNACaaa > 2:2527223/1‑72 (MQ=255) cAGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACaaa > 1:137057/1‑72 (MQ=255) aGCTGAACCGTCGGGAAANTCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAg < 1:2205676/72‑1 (MQ=255) aGCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAg < 2:3889982/72‑1 (MQ=255) gCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGt < 2:2716121/72‑1 (MQ=255) gCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGt < 1:1078108/72‑1 (MQ=255) gCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGt < 2:4760285/72‑1 (MQ=255) gCTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGt < 2:2204627/72‑1 (MQ=255) cTGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTc < 2:3456291/72‑1 (MQ=255) tGAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTcc > 1:5450883/1‑72 (MQ=255) gAACCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCg > 2:1708055/1‑72 (MQ=255) aCCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCgtg > 1:1964169/1‑70 (MQ=255) aCCGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCggg < 2:83264/72‑1 (MQ=255) ccGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGt > 2:1454026/1‑72 (MQ=255) ccGTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGt < 1:3837571/72‑1 (MQ=255) gTCGGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGa < 2:1530256/72‑1 (MQ=255) | TGGCAATGTTGAGTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAATCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGA > NC_012967/3483519‑3483656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |