| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 2,237,486 | 0 | G | A | 24.1% | 116.6 / 17.0 | 58 | intergenic (+11/+92) | ECB_RS11165/yejO | tRNA‑Pro/autotransporter YejO |
| Reads supporting (aligned to +/- strand): ref base G (14/30); new base A (0/14); total (14/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACAT > NC_012967/2237415‑2237554 | cTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAg < 1:3832946/72‑1 (MQ=255) tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑CAAAATCCC‑AAG‑a > 2:4041567/1‑72 (MQ=255) tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑a < 2:5836671/72‑1 (MQ=255) tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑a < 2:2085444/72‑1 (MQ=255) tGGTAGCGCACCGGCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑a < 2:963080/72‑1 (MQ=255) ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCCCTCGTGCCCACC‑AAAAATCCC‑CAG‑aa > 1:220652/1‑72 (MQ=255) gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑aaa < 2:980503/72‑1 (MQ=255) gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑aaa < 2:5944910/72‑1 (MQ=255) tAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑aaaa < 2:7642042/72‑1 (MQ=255) aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑aaaaa < 1:2362435/72‑1 (MQ=255) aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑aaaaa < 1:463312/72‑1 (MQ=255) gccccGTCAGGGGGTGTCGGGGTTGGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc < 1:7048540/69‑1 (MQ=255) gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑CAG‑aaaaaaac > 2:6717465/1‑70 (MQ=255) aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a > 1:6232913/1‑72 (MQ=255) ccGTCCTGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a‑c < 1:1850737/72‑1 (MQ=255) ccGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a‑c < 1:195613/72‑1 (MQ=255) cGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a‑cc < 1:2086102/72‑1 (MQ=255) cGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a‑cc > 2:3755007/1‑72 (MQ=255) cGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a‑cc < 1:5443280/72‑1 (MQ=255) cGTCAGGGGGTGCGGGGGGTCGGAGGTTAAAAC‑CCTCCCGTGCCGACA‑AAAAATCCC‑AAG‑AAAAaa‑cc‑a‑a‑cc < 1:1422110/72‑1 (MQ=255) tCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAT‑AAAAAA‑AC‑A‑A‑CCC‑t > 1:3680617/1‑72 (MQ=255) cATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑tt < 1:7178523/72‑1 (MQ=255) cATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGGGCCGACC‑AAAAATCCA‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑tt < 2:6204059/72‑1 (MQ=255) gtGGGGTGTGGGGGGCCGGAGGTCAAAAC‑CCTCCCGGGCCGACA‑AAAAACCCC‑AAA‑AAAAAA‑CC‑A‑A‑CCC‑TTa < 1:1844862/71‑1 (MQ=255) gggggTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAA‑AAAAAA‑CC‑A‑A‑CCC‑TTAc < 1:7235682/71‑1 (MQ=255) ggggTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACg < 2:4065639/72‑1 (MQ=255) gggTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg < 2:6027356/72‑1 (MQ=255) ggTGGCGGGGTTCGGAGGTTCAACC‑CCCCTCGTGCCGACA‑AAAAATCCA‑AAA‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg‑t < 1:7192158/72‑1 (MQ=255) tgtgGGGGGGCGGAGGTTAAAAC‑CCTCTCGTGCCGACA‑AAAAACCCA‑AAA‑AAAAAC‑CC‑A‑A‑CCC‑TTACgg‑ttg < 2:6421258/72‑1 (MQ=255) tgtCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg‑ttg < 1:7700948/72‑1 (MQ=255) tgtCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACA‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg‑ttg < 2:3103471/72‑1 (MQ=255) gcgcGGGGGGCGGGAGTTCAAAC‑CCCNCCGGCCCCAAC‑AAAAACCCC‑AAA‑AAAAAACCC‑A‑A‑CCC‑TTACgg‑tt < 2:843810/69‑1 (MQ=255) gtCGGGGGTCGGAGGTTAAAAC‑CCTCCCGTGCCGACA‑AAAAACCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg‑ttgg < 1:3767197/72‑1 (MQ=255) gcGGGGGTAGGAAGTTAAAAT‑CCCCCCCGGCCAAAC‑AAAAAACCCAAAA‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg‑ttgg < 1:4382391/71‑1 (MQ=255) ccGGGGGTCGGAGGTTCAAAC‑CCTCTCGGGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACgg‑ttgg‑t < 1:3635794/71‑1 (MQ=255) ggggggCGGGGGGTCCAATT‑CCTCTCGTGCCAACA‑AAAAATCCA‑AAG‑AAAAAC‑CC‑A‑A‑CCC‑TTACgg‑ttgg‑tt < 2:3689405/71‑1 (MQ=255) gggggt‑gggggtTTAAATCCCTCTCGGGCCCACC‑AAAAAACCA‑AAA‑AAAAAACCC‑A‑A‑CCC‑TTACgg‑ttgg‑tt < 2:978307/72‑1 (MQ=255) gggggTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑CTACGG‑TTGG‑ttt > 1:5882516/1‑72 (MQ=255) gggggTCGGAGGTTCAAAT‑CCTCTCGTGCCGACA‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑ttt < 2:3841193/72‑1 (MQ=255) gcgggTCGGAGTTTAAACC‑CCTCTCGTCCCGACA‑AAAAACCCA‑AAA‑AAAAAC‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑ttt < 2:2330082/70‑1 (MQ=255) gggTGGGAGG‑TCAAATCCCTCCCGGGCCG‑CC‑AAAAAACCC‑AAA‑AAAAAACCC‑A‑A‑CCC‑TTACGG‑TTGG‑tttta < 1:133157/72‑2 (MQ=255) gggTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑C‑A‑CCC‑CTACCG‑GTTG‑ttttt > 2:7371870/1‑72 (MQ=37) gttCGGAGTTTCAAAT‑CCTCTCGTGCCAACA‑AAAAACCCA‑AAA‑AAAAAC‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑tttttt < 2:6820420/70‑1 (MQ=255) ggTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑tttttt < 2:7670256/72‑1 (MQ=255) gTCGGAGGTTCAAGT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAGAAAAAAA‑CC‑A‑A‑CCC‑CTCCGG‑CTGG‑gttttt > 1:2440326/1‑72 (MQ=255) gTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAA‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑ttttttt < 2:5718524/72‑1 (MQ=255) cGGGGGTTAAAAT‑CCCCTCGTGCCGACA‑AAAAATCCA‑AAG‑AAAAAC‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTtat < 2:3720804/72‑1 (MQ=255) ggAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CCAA‑A‑CCC‑TACCGG‑TTGG‑gtttttttt > 2:3889290/1‑70 (MQ=255) ggggTTCAAAT‑CCCCCCGGGCCGACC‑AAAAAACCC‑AAA‑AAAAAACCC‑A‑C‑CCC‑TTACGG‑TTGG‑TTTTTTtata < 1:3899923/70‑1 (MQ=255) gAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑ACA‑CCC‑CTACGG‑TCGG‑tttttttttt > 2:284971/1‑69 (MQ=255) gAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTttat > 2:5680855/1‑72 (MQ=255) ggTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTatatat < 1:5627931/72‑3 (MQ=255) tttCAAAC‑CCTCTCGTGCCAACA‑AAAAACCCA‑AAA‑AAAAAC‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTg < 2:5277542/71‑1 (MQ=255) gTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTg < 1:7637649/72‑1 (MQ=255) ttCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑CTACGG‑GTGG‑GTTTTTTATATCTGc > 1:6055555/1‑72 (MQ=255) aaaT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCaat < 2:5908675/72‑1 (MQ=255) aaaT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCaat < 2:7660963/72‑1 (MQ=255) aaaT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCaat < 1:4787402/72‑1 (MQ=255) aaaC‑CCTCCCGTGCCGACA‑AAAAACCCA‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCaat < 2:5680880/72‑1 (MQ=38) aT‑CCTCCCG‑GCCCACCAAAAAATCAA‑AAA‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCaatta < 1:3622292/72‑1 (MQ=255) tctcGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAACCC‑A‑C‑CCC‑TTCCGG‑TTGG‑TTTTTTTTTTTTTGCaattaatt > 1:5522825/1‑72 (MQ=255) tctcGT‑CCCACA‑AAAAACCCA‑AAA‑AAAAAACCC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTc < 2:6629372/72‑1 (MQ=255) tcccGTGCCGA‑C‑AAAAATCCC‑AAA‑AAAAAAGCC‑A‑C‑CCT‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTc < 2:3836922/69‑1 (MQ=255) gCCGACA‑AAAAATCCA‑AAG‑AAAAAC‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTCGATaaa < 2:5788652/72‑1 (MQ=255) ccGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑CTACCG‑GTGG‑GTTTTTTTTATACTCAAATAATTTCATAAAc > 2:7466749/1‑72 (MQ=255) cGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TCACGG‑TTGG‑GTTTTTTATATCTGCAATTAATTCGATAAACa > 2:7340311/1‑72 (MQ=255) gACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑GTGG‑GTTTTTTTTATTTTCAAATTATTTCATAAACaa > 2:5677763/1‑71 (MQ=255) caaaaaaaaCCC‑AAA‑AAAAAACCC‑A‑A‑CCCTTTAGGG‑TGGG‑TTTTTTTATATCTGCAATTAATTCGATAAACa < 2:149570/69‑1 (MQ=255) ac‑AAAAAACCC‑AAA‑AAAAAACCC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTCGATAAACAGa < 1:235583/71‑1 (MQ=255) aaaaaaaaaCCC‑AAA‑AAAAAACCC‑A‑C‑CCC‑TTTAGG‑TTGGTTTTTTTTATATCTGCAATTAATTCGATAAACa < 1:588498/69‑1 (MQ=255) aaaaaaaCCC‑AAA‑AAAAAACCC‑C‑C‑CCC‑TTTGGG‑TTGGTTTTTTTTATATCTGCAATTAATTCGATAAACAGa < 1:5095041/71‑1 (MQ=255) aaaagTCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTCGATAAACAGACCg < 2:7421588/72‑1 (MQ=255) aaaaaCCCA‑AAA‑AAAAAA‑CC‑A‑ACCCC‑TTCCGT‑GGGG‑TTTTTTTATATCTGCAATTAATTCGATAAACAGAcc < 1:1452086/72‑1 (MQ=255) aaaCCCG‑AAA‑AAAAAA‑CC‑A‑A‑CCC‑AATCGGCGAGG‑TTTTTTTATATCTGCAATTAATTCAATAAACAGACCGt < 1:2336325/72‑1 (MQ=255) c‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑GTTTTTTATATCTGCAATTAATTCGATAAACAAACCGTGACACAt > 1:7606215/1‑72 (MQ=255) | CTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT‑CCTCTCGTGCCGACC‑AAAAATCCC‑AAG‑AAAAAA‑CC‑A‑A‑CCC‑TTACGG‑TTGG‑TTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACAT > NC_012967/2237415‑2237554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |