BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012967	1,003,919	G→T	N52K (AAC→AAA)	ompF ←	porin OmpF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	1,003,919	0	G	T	100.0%	564.2 / NA	164	N52K (AAC→AAA)	ompF	porin OmpF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (68/96); total (68/96)

Only 100 of 174 total aligned reads displayed.

GAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCA  >  NC_012967/1003851‑1003988

gAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTtttt                                                                    >  2:2148655/1‑72 (MQ=255)

gAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTtttt                                                                    >  2:7522381/1‑72 (MQ=255)

 aaTTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACtttttt                                                                   >  1:1280467/1‑72 (MQ=255)

 aaTTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACtttttt                                                                   <  2:6904819/72‑1 (MQ=255)

  aTTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTc                                                                  <  1:2396828/72‑1 (MQ=255)

     gATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCAcc                                                               <  1:811599/72‑1 (MQ=255)

       tttGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGt                                                             <  2:5422402/72‑1 (MQ=255)

       tttGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGt                                                             <  1:3785911/72‑1 (MQ=255)

        ttGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGtt                                                            <  2:1282981/72‑1 (MQ=255)

        ttGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGtt                                                            <  1:5539319/72‑1 (MQ=255)

         tGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTa                                                           <  1:1514124/72‑1 (MQ=255)

          gAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTAc                                                          <  2:4694168/72‑1 (MQ=255)

          gAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTAc                                                          >  1:247723/1‑72 (MQ=255)

           aGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACAGTAACTTTTTTCACCGTTAcc                                                         <  1:5554017/72‑1 (MQ=255)

            gTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTAccc                                                        <  2:5042196/72‑1 (MQ=255)

            gTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTAccc                                                        >  1:3971716/1‑72 (MQ=255)

              ttCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCtt                                                      >  2:5047173/1‑72 (MQ=255)

              ttCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCtt                                                      >  2:7586194/1‑72 (MQ=255)

               tCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCtcg                                                     >  1:42634/1‑70 (MQ=255)

               tCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTg                                                     <  2:5596461/72‑1 (MQ=255)

               tCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTg                                                     <  1:3331416/72‑1 (MQ=255)

               tCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTg                                                     >  2:2113700/1‑72 (MQ=255)

                ccccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTgg                                                    >  2:2523953/1‑72 (MQ=255)

                ccccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTgg                                                    >  2:5329164/1‑72 (MQ=255)

                  ccTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGgag                                                  <  1:151468/72‑1 (MQ=255)

                   cTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGgaga                                                 >  1:6112330/1‑72 (MQ=255)

                    tttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGaa                                                >  2:4890297/1‑72 (MQ=255)

                    tttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGaa                                                <  2:7437271/72‑1 (MQ=255)

                     ttAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGaaa                                               <  2:4586313/72‑1 (MQ=255)

                       aaaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaata                                             >  2:4741099/1‑72 (MQ=255)

                       aaaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaata                                             <  1:4984595/72‑1 (MQ=255)

                       aaaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaata                                             >  2:5784185/1‑72 (MQ=255)

                       aaaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaata                                             >  2:1968246/1‑72 (MQ=255)

                        aaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGagaaagaa                                            <  1:6438020/72‑1 (MQ=255)

                        aaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaataa                                            <  2:3835048/72‑1 (MQ=255)

                        aaaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaataa                                            >  1:168100/1‑72 (MQ=255)

                        aaaCCAAGACGGGCATAGGTCATGTCGACATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaataa                                            >  2:4101996/1‑72 (MQ=255)

                         aaCCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAaataat                                           >  1:5254698/1‑72 (MQ=255)

                           ccAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGc                                         >  2:2734116/1‑72 (MQ=255)

                             aaGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAg                                       >  1:1847470/1‑72 (MQ=255)

                              aGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGa                                      >  2:1789782/1‑72 (MQ=255)

                              aGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGa                                      <  2:5201901/72‑1 (MQ=255)

                               gACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGAc                                     >  2:6214245/1‑72 (MQ=255)

                               gACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGAc                                     >  2:6474829/1‑72 (MQ=255)

                                aCGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGAcc                                    >  2:6663771/1‑72 (MQ=255)

                                aCGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGAcc                                    <  2:6684218/72‑1 (MQ=255)

                                aCGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGAcc                                    >  1:6171750/1‑72 (MQ=255)

                                 cGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCg                                   >  2:5945126/1‑72 (MQ=255)

                                 cGGGCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCg                                   >  2:6363216/1‑72 (MQ=255)

                                  gggCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGa                                  >  1:3032805/1‑72 (MQ=255)

                                  gggCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGa                                  <  2:4312920/72‑1 (MQ=255)

                                   ggCATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGAc                                 <  1:2159285/72‑1 (MQ=255)

                                    gCATAGTTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACa                                <  1:667079/72‑1 (MQ=255)

                                     cATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAg                               <  1:7038552/72‑1 (MQ=255)

                                     cATAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAg                               <  1:3640543/72‑1 (MQ=255)

                                     cATAGGTCATGTCGCCATTGCCACCGTAACTNTTTTCANCGTTACCCTTGGAGAAATAATGCAGACCGACAg                               >  1:443099/1‑72 (MQ=255)

                                      aTAGGTCATGTCGCCATTGCCACNGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGc                              >  1:4950902/1‑72 (MQ=255)

                                      aTAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGc                              <  2:5948550/72‑1 (MQ=255)

                                       tAGGTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCt                             <  2:5547879/72‑1 (MQ=255)

                                         gctCATCTCGCCAGTGCCACCGTAACTTTTTTCGCCGTTACCCTGGGAGAAATAATGCAGGCCGACAGCttt                           <  2:7177466/70‑1 (MQ=255)

                                          gTCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTa                          >  1:3437094/1‑72 (MQ=255)

                                           tCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAc                         >  2:6140874/1‑72 (MQ=255)

                                           tCATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAc                         <  1:3873829/72‑1 (MQ=255)

                                            cATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                        >  2:2923948/1‑72 (MQ=255)

                                            cATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                        >  2:3643890/1‑72 (MQ=255)

                                            cATGTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTAcc                        >  2:2534175/1‑72 (MQ=255)

                                               gTCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTa                     <  2:3174423/72‑1 (MQ=255)

                                                tCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTAc                    >  2:3264074/1‑72 (MQ=255)

                                                tCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTAc                    >  1:7637688/1‑72 (MQ=255)

                                                tCGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTAc                    <  2:5432834/72‑1 (MQ=255)

                                                 cGCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACa                   >  1:142721/1‑72 (MQ=255)

                                                  gCCGTTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAg                  <  1:582974/72‑1 (MQ=255)

                                                  gCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAg                  >  2:7402841/1‑72 (MQ=255)

                                                  gCCATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAg                  <  2:2003491/72‑1 (MQ=255)

                                                   cctttGCCACCGTAACTTNTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGa                 <  1:2996664/69‑1 (MQ=255)

                                                   ccATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGa                 <  1:5734519/72‑1 (MQ=255)

                                                    cATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGAt                <  2:1425483/72‑1 (MQ=255)

                                                    cATTGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGAt                >  2:6588041/1‑72 (MQ=255)

                                                      ttGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCt              <  1:3077628/72‑1 (MQ=255)

                                                      ttGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCt              <  1:1598990/72‑1 (MQ=255)

                                                      ttGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCt              <  2:5706702/72‑1 (MQ=255)

                                                       tGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTa             >  2:1559097/1‑72 (MQ=255)

                                                       tGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTa             <  2:4836626/72‑1 (MQ=255)

                                                       tGCCACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTa             <  2:3410077/72‑1 (MQ=255)

                                                       tGCCACCGTAACTTTTTTCACCGNTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTa             >  1:4969302/1‑72 (MQ=255)

                                                         ccACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACt           <  1:1794384/72‑1 (MQ=255)

                                                         ccACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACt           >  1:2557621/1‑72 (MQ=255)

                                                         ccACCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACt           >  1:2605782/1‑72 (MQ=255)

                                                           aCCGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACttt         <  1:1171314/72‑1 (MQ=255)

                                                             cGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgt       <  1:7001363/72‑1 (MQ=255)

                                                             cGTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgt       >  2:5350501/1‑72 (MQ=255)

                                                              gTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgtt      <  1:2627122/72‑1 (MQ=255)

                                                              gTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgtt      <  1:92697/72‑1 (MQ=255)

                                                              gTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgtt      <  1:4154034/72‑1 (MQ=255)

                                                              gTAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgtt      <  2:5291106/72‑1 (MQ=255)

                                                               tAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgttg     >  1:3741166/1‑72 (MQ=255)

                                                               tAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgttg     <  2:1852479/72‑1 (MQ=255)

                                                               tAACTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTttgtcg     <  1:430275/72‑3 (MQ=255)

                                                                aaCTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGc    >  1:4340551/1‑72 (MQ=255)

                                                                 aCTTTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGcc   <  2:3962639/72‑1 (MQ=255)

GAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAGAAATAATGCAGACCGACAGCTTTACCGTACAGATCTACTTTGTTGCCA  >  NC_012967/1003851‑1003988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`