| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 1,132,851 | 0 | A | G | 20.6% | 526.8 / 30.4 | 240 | N65N (AAT→AAC) | yceI | YceI family protein |
| Reads supporting (aligned to +/- strand): ref base A (50/139); new base G (49/0); total (101/139) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 268 total aligned reads displayed.
TTATCGCGTTCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAGGT > NC_012967/1132780‑1132921 | ttATCGCGTTCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGa < 1:4165072/72‑1 (MQ=255) tATCGCGTTCGGCGTGATTAGTATCGACGCNGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGAt < 2:620589/72‑1 (MQ=255) aTCGCGTTCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGAtt < 2:3106656/72‑1 (MQ=255) cgcgTTCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGtttt > 1:4926679/1‑72 (MQ=255) gTTCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGAttttttt < 2:9157659/72‑1 (MQ=255) ttCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTc < 1:7254358/72‑1 (MQ=255) tCGGCGTGAGTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGGCGGGctttttttg > 1:908256/1‑70 (MQ=255) cGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGt > 1:8288531/1‑72 (MQ=255) cGGCGTGATTAGTATCGACGCCGGTGGTGTTAATTGTAACATTCACTTTATCGGCAGGCGGGTGTTTTTTGt > 2:3499183/1‑72 (MQ=255) gCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGCTTTTTTTTGTct > 1:6739669/1‑71 (MQ=255) gTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCaaa < 2:2237187/72‑1 (MQ=255) tGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAg < 2:1339372/72‑1 (MQ=255) gATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTCGTCCAAgg > 2:7514700/1‑72 (MQ=255) gATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTCGTCAAAgg > 1:2468690/1‑72 (MQ=255) gATTAGTATCGACGCTGGCGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAgg < 1:3323168/72‑1 (MQ=255) aTTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGt < 1:3256342/72‑1 (MQ=255) aTTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGt < 2:4156392/72‑1 (MQ=255) ttAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTa < 2:6681208/72‑1 (MQ=255) tAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGATTTTTTCGTCAAAGGTaa > 2:7879869/1‑72 (MQ=255) tAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTaa < 2:7840244/72‑1 (MQ=255) aGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGCTTTTTTTGTTAAAAGTaaa > 2:3893954/1‑72 (MQ=255) aGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTaaa < 2:6859044/72‑1 (MQ=255) gTATCGACCCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGGTTTTTTCGTCTAAAGTaaaa > 2:131335/1‑72 (MQ=255) tATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAg < 2:4026091/72‑1 (MQ=255) aTCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGt < 1:3665395/72‑1 (MQ=255) tCGACGCTGGTGTTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTa < 2:7723559/72‑1 (MQ=255) gACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTAcc < 1:1390213/72‑1 (MQ=255) aCGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGTCACAGGTGAAAAGACCg > 2:2799445/1‑72 (MQ=255) aCGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAAGGAAAAATAACg > 2:2202710/1‑72 (MQ=255) aCGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCCGGTTTTTTTTGTCAAAGGTAAAAGTaacc > 1:4482570/1‑69 (MQ=255) aCGCTGGTGGTGTTAATTGTCACATTCAATTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCg < 1:2704290/72‑1 (MQ=255) cGCTGGTGGTGTTAATTGTCACATTCACTTTATCTGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGt < 2:8075932/72‑1 (MQ=255) cGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGt < 1:6083123/72‑1 (MQ=255) gCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAGGTAAAAGTACCGTc > 1:2832904/1‑72 (MQ=255) gCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAGGTAAAAGTAACGTc > 2:8896933/1‑72 (MQ=255) gCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTCGTCAAAGGGAAAAATAACGTc > 1:5491494/1‑72 (MQ=255) tggtggTGTTAATTGTCACATTCACTTTATCGGCAGCCGGCCTTTTTTTTTCCAAGGTAAAAATAACGTcca > 1:2390677/1‑70 (MQ=255) tggtggTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGa < 2:3551807/72‑1 (MQ=255) ggtggtGTTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTCGTTAAAGGTAAAAATACCTTCgga > 1:5997329/1‑70 (MQ=255) gtggtgTTAATTGTCACATTCACTTTATCGGCAGCCGGGGTTTTTTTGTCAAAGGGTAAAGTACCGTCGaaa > 1:8693465/1‑72 (MQ=255) tggtgTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAAt < 2:2732011/72‑1 (MQ=255) tgtTAATTGTCTCATTCACTTTATCGGCAGCCGGGCTTTTTTTGTCAAAGGTAAAAATACCGTCGAAATCtt > 2:7346810/1‑72 (MQ=38) tgtTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCtt < 2:1428360/72‑1 (MQ=255) gtTAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAGGTAAAAGTAACGTCGAAATCttt > 2:191097/1‑72 (MQ=255) gtTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCttt < 2:2453837/72‑1 (MQ=255) ttAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTa < 1:7707773/72‑1 (MQ=255) ttAATGGTCACAGTCACTGTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTa < 1:5819691/72‑1 (MQ=255) tAATTGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAGGGAAAAGTACCGTCGAAAACTTTaa > 2:5600393/1‑72 (MQ=255) tAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTaa < 2:7447031/72‑1 (MQ=255) aaTTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTaaa < 1:9650006/72‑1 (MQ=255) atagTCACATTCACTTTATGGGCAGCCGGATTTTTTTCGTCANAGGTAAAAGTACCGTCGAAATCTTTAAAg < 2:841526/69‑1 (MQ=255) aTTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAg < 1:9116982/72‑1 (MQ=255) aTTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAg < 1:6947773/72‑1 (MQ=255) aTTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAg < 1:81655/72‑1 (MQ=255) tGTCACATTCACTTTATCGGCAGCCGGGTTTTTTTTTTTAAAAGTAAAAATAACGTCGGAAACTTTTAAAGt > 2:3261738/1‑72 (MQ=255) tGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGt < 1:7106563/72‑1 (MQ=255) tGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGt < 2:7534236/72‑1 (MQ=255) gTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTg > 1:9905947/1‑72 (MQ=255) cacaTTCACTTTATCGGCAGTCGGGTTTTTTTTGTCAAAGGGAAAAAGACCGGCGGAATCTTTAAAGGTGcc > 1:8876667/1‑72 (MQ=255) cacaTTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGcc < 2:1137637/72‑1 (MQ=255) acaTTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCg < 1:1880864/72‑1 (MQ=255) caTTCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAAGTAAAAATAACGTCGAAAACTTTTAAAGTGCCGt > 2:3360290/1‑72 (MQ=255) aTTCACTTTATCGGCAGCCGGGTTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGta > 2:9652867/1‑72 (MQ=255) tCACTTTATCGGCAGCCGGGTTTTTTTTGTCAAAGGGAAAAGTACCGTCGAAAACTTTTAAGGTGCCGtata > 2:342821/1‑72 (MQ=255) tCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGtata < 2:8836830/72‑1 (MQ=255) tCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGtata < 2:8566249/72‑1 (MQ=255) cACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATaa < 2:3794646/72‑1 (MQ=255) cACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATaa < 2:4103833/72‑1 (MQ=255) cACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGAATaa < 1:7490966/72‑1 (MQ=255) aCTTTATCGGCAGCCGGGTTTTTGTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTTAAAGTGCCGTATAAc > 1:6324888/1‑72 (MQ=255) tttATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCa < 2:5445140/72‑1 (MQ=255) tATCGGCAGCCGTATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGc > 1:3524487/1‑72 (MQ=255) tATCGGCAGCCGGGTTTTTTTCGTCNAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGc > 1:1095702/1‑72 (MQ=255) tATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGc > 1:9264482/1‑72 (MQ=255) aTCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAATTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCt < 1:5423232/72‑1 (MQ=255) aTCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCCGCt > 1:2347225/1‑72 (MQ=255) aTCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCt < 1:9575639/72‑1 (MQ=255) aTCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCt < 1:2764687/72‑1 (MQ=255) aTCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGGACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCt > 2:9456604/1‑72 (MQ=255) tCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCta > 2:7314322/1‑72 (MQ=255) tCGGCAGCCGGATTTTTTTAGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCta < 1:318946/72‑1 (MQ=255) cGGCAGCCGGGTTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGGGCCGTATAACCAGCtat > 1:4069786/1‑72 (MQ=255) ggCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCtata > 1:856968/1‑72 (MQ=255) ggCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCtata > 1:4306356/1‑72 (MQ=255) ggCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCtata < 1:3852118/72‑1 (MQ=255) gaggCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCGTTAAAGGTGCCGTATAACCAGCTATAg < 1:1801728/69‑1 (MQ=255) cAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATNTTTAAAGGTGCCGTATAACCAGCTATAGc < 2:484919/72‑1 (MQ=255) cAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGc > 2:4779059/1‑72 (MQ=255) cAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGc > 2:101611/1‑72 (MQ=255) cAGCCGGACTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGc > 2:6425340/1‑72 (MQ=255) aGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGcc < 2:4488341/72‑1 (MQ=255) gCCGGATTTTTTTCGTCAAAGGTAAAAGTNCCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCa > 2:493037/1‑72 (MQ=255) ccGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCaa > 1:4706354/1‑72 (MQ=255) cGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCCGCTATAGCCAAg > 1:7915390/1‑72 (MQ=255) cGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAg < 1:5321948/72‑1 (MQ=255) cGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAg < 1:1308206/72‑1 (MQ=255) cGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAg > 2:1629390/1‑72 (MQ=255) cGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAACCTTTAAAGGTGCCGTATAACACGCTATAGCCAAg > 1:294910/1‑72 (MQ=255) gATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAGGt < 2:3701115/72‑1 (MQ=255) gATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAGGt < 2:6076838/72‑1 (MQ=255) | TTATCGCGTTCGGCGTGATTAGTATCGACGCTGGTGGTGTTAATTGTCACATTCACTTTATCGGCAGCCGGATTTTTTTCGTCAAAGGTAAAAGTACCGTCGAAATCTTTAAAGGTGCCGTATAACCAGCTATAGCCAAGGT > NC_012967/1132780‑1132921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |