| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 2,016,091 | 0 | T | A | 29.7% | 66.5 / 16.6 | 37 | intergenic (‑123/+56) | yeeE/plaP | thiosulfate utilization transporter TsuA/YeeE/putrescine/proton symporter PlaP |
| Reads supporting (aligned to +/- strand): ref base T (12/14); new base A (0/11); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTACGTCTT > NC_012967/2016020‑2016160 | aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAAt < 1:1708575/72‑1 (MQ=255)aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAAt < 1:306267/72‑1 (MQ=255)aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAAt > 1:4498267/1‑72 (MQ=255) aTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa > 2:2077843/1‑72 (MQ=255) gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aa < 2:3735028/72‑1 (MQ=255) gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aaaaa < 1:1595046/72‑1 (MQ=255) gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aaaaa > 2:4961128/1‑72 (MQ=255) gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aaaaa < 1:5067151/72‑1 (MQ=255) gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aaaaa > 1:4494557/1‑72 (MQ=255) aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aaaaaa > 2:7693901/1‑72 (MQ=255) cAATANCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aacaaaac > 1:862618/1‑70 (MQ=255) cAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑cc < 1:4503112/72‑1 (MQ=255) aaTAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATa‑aaaaaaaca > 2:5390804/1‑69 (MQ=255) aTAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑cacc > 2:7741707/1‑69 (MQ=255) tAACCAAATTCTCTAAATTAGAACAAAGGGTTATTAATGAGGATTTCGATAAAAAAGAAAA‑AAAAAA‑CCGGa < 2:5844577/72‑1 (MQ=255) aaCAAAATTCTCTAAATAAGAACAAATGTTTATTAAGGAGAATTTCGAAAAAAAAAAAAA‑AAAAAA‑CCGGAg < 1:3202923/72‑1 (MQ=255) aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGc < 1:3963554/72‑1 (MQ=255) aaaaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCa < 2:6282353/71‑1 (MQ=255) aaaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGACTACAAAAAA‑CCGGAGcac > 2:6549912/1‑70 (MQ=255) aaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAACCCGGGGCCaa > 2:6300591/1‑72 (MQ=255) aaTTCTATAAATTAGAAAAAATGTTTATTAATGAGGATTTCGAAAAAAAAGAAAA‑AAAAAA‑CCGGAGCCaaa < 2:4059026/72‑1 (MQ=255) aTTCTCAAAATAAGAAAAAATGTTTATAAAGGAGAATTTCGAAAAAAAAAAAAA‑AAAAAA‑CCGGAGCCAAat < 1:8136016/72‑1 (MQ=255) ttCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCCGGGGCaaaaa > 2:8463824/1‑70 (MQ=255) tctAAATTAGAAAAAATGTTTATTAATGAGGATTTCAAAAAAAAAAAAAA‑AAAAAA‑CCGGAGCCAAATattc < 1:4028703/72‑1 (MQ=255) aaaTTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATattcatt > 2:6992121/1‑72 (MQ=255) aaaTTAGAAAAAATGGTTATTAATGAGGATTTCGAAAAAAAAAAAAA‑AAAAAA‑CCGGAGCCAAATattcatt < 2:2135817/72‑1 (MQ=255) aaTTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATattcattc < 1:4906536/72‑1 (MQ=255) aaTTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATattcattc < 2:7314835/72‑1 (MQ=255) aaaaaaTGGTTATTAATGAGGATTTCGATAAAAAAAAAAA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑cc < 2:6290352/69‑1 (MQ=255) aaaaaTGTTAATAAAGGAGGATTTCAAAAAAAAAAAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCg < 1:6429526/70‑1 (MQ=255) aaaTGTTTATTAATGAGGATTTCAATAAAAAAGAAAA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGt < 2:6176762/72‑1 (MQ=255) aagggTTATTAAGGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGtt < 2:7316353/69‑1 (MQ=255) aTGGTTATTAAGAAGGATTTCAATAAAAAAGAAGA‑AAAAAC‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTg < 2:6979821/72‑1 (MQ=255) ggTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGat < 2:9213087/72‑1 (MQ=255) gTTATTAATAAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGatt < 2:6742938/72‑1 (MQ=255) gTTATTAAAGAAGATTTTAAAAAAAAAAAAAA‑AAAAAACCCGGAGCCAAATATTCATTCGACT‑CCGGTTGat < 1:4040972/72‑1 (MQ=255) ataaaTGAGGATTTCAAAAAAAAAAAAAA‑AAAAAC‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGattatt < 1:4173168/69‑1 (MQ=255) ttaATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑GCGGTTGAttatta < 1:6933275/72‑1 (MQ=255) aaTGAGGATTTCGATAAAAAAAAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGa < 2:2401633/72‑1 (MQ=255) ggATTTCGATAAAAAAGAATA‑AAAAAACCCGGGGCCAAA‑AATCTTTTTACTCCCGGTTGGTTTTTTGaaaaa > 1:8928496/1‑71 (MQ=255) ggATTTAGAAAAAAAAAAAAA‑AAAAAACCCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAg < 1:1643288/72‑1 (MQ=255) aaaaaaaaaaaaaa‑aaaaaC‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTAt < 1:8076177/69‑1 (MQ=255) aaaaaaaaaaaa‑aaaaaC‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGc < 2:8274469/71‑1 (MQ=255) aaaaaaaaa‑aaaaaC‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTAc < 1:395662/72‑1 (MQ=255) aaaaGAAAA‑AAAAAC‑CCGGAGCNAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTAc < 1:2562362/72‑1 (MQ=255) aaaGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTACg < 2:8578120/72‑1 (MQ=255) aGAAAA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTACGTc < 1:4589930/72‑1 (MQ=255) aaTA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTACGTCtt < 1:2423118/72‑1 (MQ=255) | AATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGATTTCGATAAAAAAGAATA‑AAAAAA‑CCGGAGCCAAATATTCATTCGACT‑CCGGTTGATTATTAGAAAAGATGATTATGCTACGTCTT > NC_012967/2016020‑2016160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |