| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_012967 | 1,459,022 | 0 | T | G | 25.0% | 187.4 / 11.6 | 104 | intergenic (+20/+22) | aldA/gap | aldehyde dehydrogenase/type I glyceraldehyde‑3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (57/21); new base G (26/0); total (83/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 147 total aligned reads displayed.
CATGGCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACGAAGC > NC_012967/1458952‑1459093 | cATGGCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGtt < 2:3417172/72‑1 (MQ=255) aTGGCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGttt < 2:7212539/72‑1 (MQ=255) aTGGCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGttt < 2:8583462/72‑1 (MQ=255) tGGCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGtttt < 1:2702392/72‑1 (MQ=255) ggCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGttttt > 1:6324189/1‑72 (MQ=255) ggCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGttttt < 2:5299678/72‑1 (MQ=255) gCCTGCATGAATATCTGCAGACCCAGGTGAGTTATTGACAGTCGTAATGAGTAAAATA‑GAC‑GGGGGtttttt < 2:8150514/72‑1 (MQ=255) ccTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGggttttt > 1:1847283/1‑71 (MQ=255) cTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTtcc < 1:1954554/72‑1 (MQ=255) cTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTtcc > 1:8425587/1‑72 (MQ=255) cATGAATATCTGCAGACCNAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTtcctcc < 1:3402960/72‑1 (MQ=255) aTGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCg < 1:3532048/72‑1 (MQ=255) tGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGc < 2:2965348/72‑1 (MQ=255) tGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGc > 2:6774361/1‑72 (MQ=255) gAATATCTGCAGACCAAGTTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGcc < 2:6246462/72‑1 (MQ=255) aaTATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGccc > 2:1966472/1‑71 (MQ=255) atatCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGCGGGGGGTTTTTTCCTCccccg > 1:1653935/1‑71 (MQ=255) tatCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTTCTCCGTCtgt > 2:2292631/1‑72 (MQ=255) tCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTgc > 2:8664673/1‑72 (MQ=255) cTGGAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTgcg < 2:8529857/72‑1 (MQ=255) cTGCAGCCCAGGGTGGTTTATTTACAGTCGTAAGGAGAAAAAGG‑GGC‑GGAGGTTTTTCCCCCCGCCTGTgcg < 1:2977902/72‑1 (MQ=255) cTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGGGGGTTTTTTCTCCGCCCTTgcg > 2:5617574/1‑72 (MQ=255) cTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTgcg > 2:3819453/1‑72 (MQ=255) cTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTgcg > 1:7080463/1‑72 (MQ=255) cAGACCCAGGTGGTTTATTCACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCCTGTGCGCG‑t > 2:6986484/1‑72 (MQ=255) aGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGGGGGTTTTTTCTCCCGCTGTTcgcggt > 2:1574490/1‑70 (MQ=255) aGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑Tc > 2:1692180/1‑72 (MQ=255) gACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGGGGGTTTTTTCTCCGCCCTTGCTCG‑Tcc > 1:4552319/1‑71 (MQ=255) aCCCAGGTGGTTTATTTACAGTCGTAAGGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCag < 2:6472761/72‑1 (MQ=255) cccAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTTCTCCGCCTGTGCGCG‑GCaga > 1:5521361/1‑72 (MQ=255) cAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑TGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCTGAGt > 1:3453282/1‑72 (MQ=255) cAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGt > 1:8304088/1‑72 (MQ=255) gtggtTTCTTAACAGTCGAAATGAGAAAGAGC‑GGG‑GGAGGTTTTCTCCCCCGCCTGTGCGCG‑TCAGAGTTTa < 2:1847283/72‑1 (MQ=255) gtggtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTTCTCCCCCTTTGGGCG‑ACAGTGTTTa > 1:1365127/1‑72 (MQ=255) tgtttttttaaCGGTCGAAAGGAGAAAAAGG‑GGC‑GGAGGTTTTTCCCTCCGCCTGTGCGCG‑TCAGAGTTTAg < 2:6602526/69‑1 (MQ=255) tggtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCCTGTGCGTG‑TCAGAGttttg > 1:2771988/1‑70 (MQ=255) ggtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGc > 2:7136435/1‑72 (MQ=255) ggtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGc > 1:8593335/1‑72 (MQ=255) ggtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGc > 2:8602805/1‑72 (MQ=255) ggtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTTCTCCCCCCGTTCGCG‑GCAGAGgtttgg > 1:7575652/1‑69 (MQ=255) ggtTTATTTACAGTCGCAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCCTGTGCGCG‑TCAGAGttttgt > 1:921091/1‑69 (MQ=255) gtTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCCGAGTTTAGCg > 2:5883656/1‑72 (MQ=255) ttatttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGac > 1:3907015/1‑71 (MQ=255) tatttaCATTCAAAATGAGAAAAAGG‑GGG‑GGGGGTTTTTCCCCCCGCCTGTGCGCG‑TCAGAGTTTAGCGAAt < 2:168444/72‑1 (MQ=255) tatttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGGGCG‑TCAGCGTTTAGCGAAt > 2:2381722/1‑72 (MQ=255) tatttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAAt > 1:4217554/1‑72 (MQ=255) atttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGGTTAGCGAAtt > 1:6853789/1‑72 (MQ=255) tttaCGGTCGTAAGGAGAAAAGGG‑GGG‑GGAGTTTTTTCCCCCCGCCTGTGCGCG‑TCAGAGTTTAGCGAAttt < 2:8760735/72‑1 (MQ=255) tttaCATTCGTAATGATAAAAAGA‑GCC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAAttt < 2:6335346/72‑1 (MQ=255) tttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCATGTGCGCG‑TCAGAGGTTAGCGAAttt > 2:3310981/1‑72 (MQ=255) ttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTTGCGAAtttt > 2:3299669/1‑72 (MQ=255) ttaCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCAGCCTGTGCGCG‑TCAGAGTTTAGCGAAtttt < 1:5286268/72‑1 (MQ=255) taaCAGTCGTAATGAAAAAAAGA‑GGC‑GGAGTTTTTTCCCCCCGCCTGTGCGCG‑TCAGAGTTTAGCGAAtttt < 2:7774684/70‑1 (MQ=255) taCAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCCTGTGCGCG‑TCAGGGGTTAGCGAAttttt > 1:4778008/1‑72 (MQ=255) gTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTTCTCCGGCTGTGTGCT‑CTAGGGTTTTGGGGATTTNTcaga > 2:3381779/1‑69 (MQ=255) gTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAg > 1:6611690/1‑72 (MQ=255) tCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAgg > 1:8645497/1‑72 (MQ=255) cGTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCCTGTGCGTG‑GCCGGGGTTAGCGGATTTTTTGAggg > 1:893797/1‑72 (MQ=255) gTAATGAGTAAACGA‑GGC‑GGAGGGTTTTTTCTCCGCCCGTGCGCGTTCGGAGTTT‑GCGGATTTTTCTAGGGt > 2:573952/1‑72 (MQ=255) gTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCCGAGTTTAGCGAATTTTTCGAGGGt > 1:4687379/1‑72 (MQ=255) gTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGt > 2:4454486/1‑72 (MQ=255) gTAATGAGTAAAAGA‑GGC‑GGAGGGTTTTTCCTCCGCCTGTGCGCG‑GCAGAGTTTAGCGAATTTTTCGAgggg > 1:3700160/1‑71 (MQ=255) tAATGATTAAAAGG‑GGC‑GGAGGTTTTTTCCCCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTg < 2:6743787/72‑1 (MQ=255) aaTGAGTAAAAGAGGGG‑GGGGGTTTTTTCCCCCCCCTGGTCCCG‑‑CCCAGGTTAGCGGATTTTTCTCGGGttc > 2:1801993/1‑70 (MQ=255) aTGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCg > 1:8452691/1‑72 (MQ=255) aTGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAAATTTTTGGGGGGGCg > 2:103668/1‑72 (MQ=255) gAGTAAAAGC‑GGC‑GGGGGGTTTTTTCTCCNCCTGTGCGCG‑TCAGGGTTTTACGCAATTTTTGAGGGTGCGaa > 2:750307/1‑72 (MQ=255) gAGTAAAAGA‑GGC‑GGGGGGTTTTTTCTCCCCCCGTGCGCG‑TCCGGGGTTTGCGGATTTTTTGAGGGGGCgga > 1:1574815/1‑70 (MQ=255) gAGTAAAAGA‑GGC‑GGCGGGTTTTTCCTCCGCCCGTGCGCG‑GTAGAGCTTACGGGATTTTTCGCGGGTGCgga > 2:3849707/1‑70 (MQ=255) aGTAAAAGA‑GGC‑GGGGGGTTTTTTCTCCGCCTGTGCGCG‑GCAGAGGTTTGCGAATTTTTTGAGTGTGCggaa > 1:4951778/1‑69 (MQ=255) aGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAAt > 2:2698939/1‑72 (MQ=255) gTAAAAGA‑GGC‑GGGGGGTTTTTTCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGGATa > 2:4813289/1‑72 (MQ=255) gTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATa < 2:8158063/72‑1 (MQ=255) tAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATaa > 1:2558917/1‑72 (MQ=255) tAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATaa > 1:391891/1‑72 (MQ=255) tAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATaa > 2:6867826/1‑72 (MQ=255) aaaaGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAg > 1:6305881/1‑72 (MQ=255) aaaGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGc > 1:753054/1‑72 (MQ=255) aaGG‑GGC‑GGCGGTTTTTTCCCCCGCATGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCt < 1:1811911/72‑1 (MQ=255) aga‑gGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTg < 1:8288524/72‑1 (MQ=255) ga‑gGC‑TGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGc > 1:4401491/1‑72 (MQ=255) a‑gGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCg > 1:6712500/1‑72 (MQ=255) a‑gGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCg > 2:7764974/1‑72 (MQ=255) a‑gGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCg > 2:7368838/1‑72 (MQ=255) ggC‑GGAGGTTTTTTCCTCCGCCCGTGCGCG‑TCCGCGGTTTACGAACTTTTTGTGGGGGCCGATAACATGCGt > 2:4151646/1‑72 (MQ=255) ggC‑GGAGGGTTTTTCCTCCGCCCGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCgg > 2:1465431/1‑71 (MQ=255) gC‑GGAGTTTTCTCCCGCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTg < 2:6045894/72‑1 (MQ=255) gC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGGTGCGTg > 2:1099926/1‑72 (MQ=255) gC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTg < 2:7015953/72‑1 (MQ=255) c‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGa > 2:8492023/1‑72 (MQ=255) c‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGa > 1:1951981/1‑72 (MQ=255) ggAGGTTTTTTCCTCCTCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGAc > 1:3986743/1‑72 (MQ=255) ggAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTTGAGGGTGCGAATAAGGTGCGTGAc > 2:5172763/1‑72 (MQ=255) ggAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGAc > 1:7823071/1‑72 (MQ=255) ggAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGAc > 1:7956015/1‑72 (MQ=255) gAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACg > 1:7693387/1‑72 (MQ=255) gggTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACGa < 2:2490622/71‑1 (MQ=255) tttttttCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACGAAg < 2:1771685/71‑1 (MQ=255) ttttttcCCCCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACGAAg < 2:2884012/71‑1 (MQ=255) ttttttCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACGAAGc > 2:1635278/1‑72 (MQ=255) | CATGGCCTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCGTAATGAGTAAAAGA‑GGC‑GGAGGTTTTTTCCTCCGCCTGTGCGCG‑TCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGCGTGACGAAGC > NC_012967/1458952‑1459093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |