Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0020941,097,0000AG39.0% 100.7 / 41.2 82G207G (GGA→GGG) LLNZ_058402‑dehydropantoate 2‑reductase
Reads supporting (aligned to +/- strand):  ref base A (16/34);  new base G (32/0);  total (48/34)
Fisher's exact test for biased strand distribution p-value = 4.24e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTATTCTGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGATATAGATGA  >  CP002094/1096904‑1097098
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cTATTCTGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGAtt                                                                                                  >  2:1462196/1‑99 (MQ=255)
    tCTGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTgggg                                                                                              >  1:1466716/1‑98 (MQ=255)
    tCTGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGa                                                                                              <  1:1239904/99‑1 (MQ=255)
    tCTGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGa                                                                                              <  1:796669/99‑1 (MQ=255)
      tGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGc                                                                                            >  2:860031/1‑99 (MQ=255)
         tAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGcaac                                                                                         >  1:505270/1‑99 (MQ=255)
           aCATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGcaacaa                                                                                       <  2:1275578/99‑1 (MQ=255)
              tCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAACaaaaa                                                                                    >  1:1276287/1‑98 (MQ=255)
              tCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCGACaaaaa                                                                                    >  1:1122244/1‑98 (MQ=255)
               ccATTATTCAATTTACCGAAAAGCGTTGGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAAcc                                                                                   <  2:1030071/99‑1 (MQ=255)
                 attattCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGAGCAACAAAACCTg                                                                                 >  1:897089/1‑99 (MQ=255)
                 attattCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTg                                                                                 <  2:186760/99‑1 (MQ=255)
                  ttattCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTTGGGGGAAAAAAAACTGc                                                                                >  1:1318828/1‑99 (MQ=255)
                  ttattCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGc                                                                                <  2:215219/99‑1 (MQ=255)
                  ttattCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGAGTGGGGGGCACAAAACCTGc                                                                                >  1:543884/1‑99 (MQ=255)
                   tattCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCt                                                                               <  2:1591856/99‑1 (MQ=255)
                    attCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAAAAAAAACTGCTc                                                                              >  2:1113992/1‑99 (MQ=255)
                     ttCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAAAAAAAACTGCTCa                                                                             >  1:576221/1‑99 (MQ=255)
                     ttCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCa                                                                             <  2:1613885/99‑1 (MQ=255)
                     ttCAATTCACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCATCAAAACCGGCtta                                                                             <  2:1286644/99‑3 (MQ=255)
                      tCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCAc                                                                            <  1:860031/99‑1 (MQ=255)
                        aaTTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGa                                                                          <  1:1344566/99‑1 (MQ=255)
                         aTTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAAAAAAACCCGCTCACGat                                                                         >  1:1410887/1‑99 (MQ=255)
                          tttACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGata                                                                        <  1:633218/99‑1 (MQ=255)
                            tACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATg                                                                      <  2:1261916/99‑1 (MQ=255)
                            tACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATg                                                                      <  1:1241811/99‑1 (MQ=255)
                             aCCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGAGCAACAAAACCTGCTCACGATATgg                                                                     >  2:1030303/1‑99 (MQ=255)
                              ccGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAAACAAAACTGCTCACGATATGgt                                                                    >  1:1446022/1‑99 (MQ=255)
                                gAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGgttg                                                                  <  1:1455194/99‑1 (MQ=255)
                                 aaaaGCGTGTGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAAACTGCTCACGATATGgttgt                                                                 >  2:605794/1‑99 (MQ=255)
                                   aaGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTa                                                               <  1:354217/99‑1 (MQ=255)
                                     gcgtgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCAACAAAACCTGCTCACGATATGGTTGTTTCa                                                             >  2:1185924/1‑99 (MQ=255)
                                     gcgtgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGAGTGGGAGGGAGAAAACCAGCTCACGATATGGGTGTTACa                                                             >  2:1502983/1‑99 (MQ=255)
                                      cgtgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCAACAAAACCTGCTCACGATATGGTTGTTACaa                                                            >  2:200532/1‑99 (MQ=255)
                                      cgtgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACaa                                                            <  1:130123/99‑1 (MQ=255)
                                       gtgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCAACAAAACCTGCTCACGATATAGTTGTTACAAt                                                           >  2:1178134/1‑99 (MQ=255)
                                        tgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGGTGGGGGGAACAAAAACTGCCCACGATAAGGTTGGTTCAAtt                                                          >  1:626054/1‑99 (MQ=255)
                                        tgcgtTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACaaat                                                          >  2:816546/1‑97 (MQ=255)
                                           gtTAATGGGACAATGAATGGACTCTGCACTAGTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTc                                                       <  2:171373/99‑1 (MQ=255)
                                            ttAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAACAAAAACTGCTCACGATATGGGTGTTACAATTTTCa                                                      >  1:1135553/1‑99 (MQ=255)
                                            ttAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGAGCAACAAAAACTGCTCACGATATGGTTGTTACAATTGTCa                                                      >  2:1360237/1‑99 (MQ=255)
                                            ttAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCa                                                      >  2:1489623/1‑99 (MQ=255)
                                             tAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCaa                                                     <  2:1639612/99‑1 (MQ=255)
                                              aaTGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGGTGGGGGCAACAAAAACTGCTCACGATATGGGTGTTTTAATTGTTaaa                                                    >  1:929351/1‑99 (MQ=255)
                                              aaTGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCaaa                                                    <  2:1574807/99‑1 (MQ=255)
                                               aTGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGGTGGGGGGAACAAAAACCGCTCACGATATTGTTGTTTCAATTGTTaaaa                                                   >  1:45999/1‑98 (MQ=255)
                                                tGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCAAAAAAAACCGCTCACGATATGGTTGTTACAATTGTCaaaaa                                                  >  1:639495/1‑97 (MQ=255)
                                                tGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGGTGGGGGCAAAAAAAACTGCCCACGATATGGTTGTTTCAATTTTGaaaaa                                                  >  1:788239/1‑97 (MQ=255)
                                                 gggACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGaa                                                 <  2:448110/99‑1 (MQ=255)
                                                 gggACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGaa                                                 <  1:925116/99‑1 (MQ=255)
                                                     cAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGGAAAAAAAACCGCTCACGAAATGGTTGTTTTAATTTTCAAAAAATTTg                                             >  1:492042/1‑99 (MQ=255)
                                                     cAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGGGTGGGGGGGACAAAAACTGCTCACGATATGGTTGTTTCAATTTTCAAAGAAattt                                             >  1:492121/1‑98 (MQ=255)
                                                        tgaatgTACTCTGTATTATTTTGGATATTAATATGGCTGGATTGGGAGCAACAAACCCTCCTCCCTTTTTGTTTTTTACTATTGTCAAAGAATTTGCgg                                          <  2:299972/99‑1 (MQ=255)
                                                        tgaatgGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAAATGTTAAAGAATTTTCgg                                          >  2:1380565/1‑99 (MQ=255)
                                                           atgGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAAAATTTTCGGCAg                                       >  2:111433/1‑99 (MQ=255)
                                                            tgGACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGGGCAACAAAACCCGCTCACGATATGGGTTTTAAAATTTTTAAAAAAATTTGGGCAgg                                      >  1:1079741/1‑98 (MQ=255)
                                                             ggACTCTGCACTATTTTGGATACTAATATGGCTGGGTTGGGAGCAACAAAAACTGCTCACGATATGGTTGTTACAATTTTCAAAGAATTTTCGGCAGtt                                     >  2:367583/1‑99 (MQ=255)
                                                                   tGCACTATTTTGGATACTAATATGGCTGGATTTGGAGCAACAAAACCTGCTCACGATATGGTTGTTATAATTGTCAAATAATTTTGGGCAGTTGCaaaa                               >  2:1124705/1‑99 (MQ=255)
                                                                     cACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACa                             <  1:783213/99‑1 (MQ=255)
                                                                       cTATTTTGGATACTAATATGGCTGGGTTGGGGGGAAAAAAAACTGCTCACCATATGGTTGTTACAATTTTTAAAAAATTTTCGGGAGTTGGAAAACaag                           >  1:1040024/1‑97 (MQ=255)
                                                                         aTTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAAACTGCTCACGATATGGTTGTTACAATTGTCAAAAAATTTGCGGCAGTTGCAAAACAagag                         >  2:1191533/1‑99 (MQ=255)
                                                                             tGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGAtg                     <  1:544173/99‑1 (MQ=255)
                                                                                aTACTAATATGGCTGGGGTGGGGGGAACAAAAACTCCTCACGATATGGGTGTTTCTAATGTTAAAGAAATTGCGGGAGTTGTGAAACATTAGGGAGTGa                  >  1:800879/1‑99 (MQ=255)
                                                                                aTACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGTGGCAGGTGCAAAACATGAGGATGTGa                  >  2:469544/1‑99 (MQ=255)
                                                                                 tACTAATATGGCTGGGTTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTTCGGCAGTTGCCAAACATTAGGATGTGaa                 >  2:926246/1‑99 (MQ=255)
                                                                                 tACTAATATGGCTGGGTTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAAAATGAGGATGTGaa                 >  2:1529503/1‑99 (MQ=255)
                                                                                 tACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGaa                 <  1:1536573/99‑1 (MQ=255)
                                                                                 tACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGaa                 <  2:431310/99‑1 (MQ=255)
                                                                                  aCTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAAt                <  1:1634386/99‑1 (MQ=255)
                                                                                   cTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATc               <  2:840294/99‑1 (MQ=255)
                                                                                   cTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATc               <  2:560290/99‑1 (MQ=255)
                                                                                       tatGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAATACATGAGGATGTGAATGTTGa           >  1:782906/1‑99 (MQ=255)
                                                                                       tatGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGa           <  1:296418/99‑1 (MQ=255)
                                                                                       tatGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGa           <  2:470325/99‑1 (MQ=255)
                                                                                        atGGCTGGGTTGGGAGCAAAAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAAAAATTGCGGCAGTTGCAAAACATGAGGATGTGAATATTTat          >  2:1582472/1‑99 (MQ=255)
                                                                                         tGGCTGGATTGGGGGCAACAAAACCTGCCCACGATATGGGTGTTACCATTGTCAAAGAATTTTCGGCAGGTGCAAAACATGAGGATGGGAATCTTGata         >  2:1234165/1‑99 (MQ=255)
                                                                                         aggttGGATTGGGAGCACCAAAAAGTGCTCTCTATCTGGTTTTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAACCATGAGGATGTCAATCTTGata         <  2:1054370/95‑1 (MQ=255)
                                                                                           gCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGAtata       >  1:114521/1‑99 (MQ=255)
                                                                                           gCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGAtata       <  2:929351/99‑1 (MQ=255)
                                                                                              ggATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGATATAGAt    >  2:731165/1‑99 (MQ=255)
                                                                                              ggATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGATATAGAt    >  2:441929/1‑99 (MQ=255)
                                                                                               gATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGATATAGATg   >  2:398999/1‑99 (MQ=255)
                                                                                                aTTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGATATAGATGa  >  1:1387907/1‑99 (MQ=255)
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CTATTCTGATAACATCCATTATTCAATTTACCGAAAAGCGTGCGTTAATGGGACAATGAATGGACTCTGCACTATTTTGGATACTAATATGGCTGGATTGGGAGCAACAAAACCTGCTCACGATATGGTTGTTACAATTGTCAAAGAATTTGCGGCAGTTGCAAAACATGAGGATGTGAATCTTGATATAGATGA  >  CP002094/1096904‑1097098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑