| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP002094 | 1,904,623 | 0 | C | A | 45.6% | 75.8 / 58.0 | 79 | L162F (TTG→TTT) | LLNZ_09895 | exodeoxyribonuclease V alpha chain |
| Reads supporting (aligned to +/- strand): ref base C (31/12); new base A (0/36); total (31/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTAACTTCTTCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTAGAGTTAATAAAC > CP002094/1904527‑1904720 | tttAACTTCTTCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCaa > 2:1079286/1‑99 (MQ=255) tAACTTCTTCCACAAGTTGATAAGGCTTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTAGAAAAGTGAGTTTACTTGGCAAGc > 1:550142/1‑99 (MQ=255) tAACTTCTTCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGc < 2:217974/99‑1 (MQ=255) aCTTCTTCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCa > 1:976438/1‑99 (MQ=255) cttcttCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCAt > 2:514788/1‑99 (MQ=255) cttcttCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCAt > 1:1188940/1‑99 (MQ=255) ttcttcCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATa < 1:1169255/99‑1 (MQ=255) ttcttcCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATa > 1:304137/1‑99 (MQ=255) ttcCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATActc > 2:189309/1‑99 (MQ=255) cacaAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGc > 2:246503/1‑99 (MQ=255) aaaGTTGATAAGGATTCCCCTAATTTTTTTCAATCGTTCCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTATTTGCAAAGCCATACTCTGCaa < 2:32752/98‑1 (MQ=255) aaGTTGATAAGGTTTTCCCTCAATTTTTCAAATCGTTTCC‑TTTTTGAACAACTAATAGATTTAAAAAGTGAGTTTACTTGGAAAGCCATACTCTGCAAg < 2:1152638/99‑1 (MQ=255) aGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGc > 1:1270306/1‑99 (MQ=255) gATAAGTTTTTCCCTCATTTTTTTCATTTTTTTCC‑TTTTTGTACAACTAATAGATTTAAAAAGTGATTTTATTTGGAAAGCCATACTCTGCAAGCTTgg < 2:1408249/99‑1 (MQ=255) gATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTAAAAAGTGATTTTACTGGGCAAGCCATACTCTGCAAGCTTgg < 2:1561893/99‑1 (MQ=255) aTAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGt > 2:1363880/1‑99 (MQ=255) tAAGGTTTCCCCTTATTTTTTTAAATCGTTTCC‑TCTTTGTACAACTCATAGTTTTAAAAAGTGAGTTTACTTGGAAAGCCATACTCTGCAAGCTTGGTc < 2:228172/99‑1 (MQ=255) agtaTTTCCCTCAATTTTTTCATTCGTTCCC‑TTTTTGAACAACTAATAGATTAAAAAGGTGATTTTCCTTGAAAAGCCATACTCTGCAAGCTTGGTCAg < 1:1203853/96‑1 (MQ=255) agtaTTTCCCTCAATTTTTTCAATCGTTTCC‑TTTTTGTACAACTCATAGATTAAAAAAGTGAGTTTACTGGGAAAGCCATACTCTGCAAGCTTGGTCAg < 2:259771/96‑1 (MQ=255) ggATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGc > 1:1409179/1‑99 (MQ=255) ttctCCCCCTATTTTTTTCATCCTTTCCTTTTTTGTAAACCTCATAGTTTGAAAAAGTGATTTTCTTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCa < 1:1223301/96‑1 (MQ=255) aTTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCAc > 1:112793/1‑99 (MQ=255) tttCCCTAATTTTTTTCAATCGTTTCC‑TCTTTTTACAACTCATAGATTTGAAAAGTGTGTTTTCTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACt < 2:916112/99‑1 (MQ=255) ttCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACtt > 2:192033/1‑99 (MQ=255) ttCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACtt > 2:1150159/1‑99 (MQ=255) ccccTCATTTTTTTAAATCTTTTCC‑TTTTTGAACAACTCATAGTTTTAAAAAGTGTGTTTATTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACttt < 2:700833/98‑1 (MQ=255) cccTCAATTTTTTAAATCTTTTCC‑TCTTTGTACACCTCATAGATTTAAAAATGGATTTTACTTGACAAGCCATACTCTGCAAGCTTGGTCAGCACtttt < 2:117275/99‑1 (MQ=255) ctaaaTTTTTTCAATCGTTTCT‑TTTTTGTACAACTAAAAGATTGGAAAAGTGATTTTCCTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTc < 2:589831/96‑1 (MQ=255) cTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTc > 1:543704/1‑99 (MQ=255) catttttttCAATCTTTTCC‑TTTTTTTACAACTCATAGATTTGAAAAGGGTTTTTTCTGGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCa < 1:1521318/96‑1 (MQ=255) tttttaCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTAAAAAGTGATTTTTTTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATAc < 2:1650057/99‑1 (MQ=255) ttttCAATCGTTTCC‑TTTTGGAAAAACTAATAGATTGAAAAAGTGATTTTTCTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCa < 1:1158995/99‑1 (MQ=255) tttCAATCGTTTCT‑TTTTTGTACAACTCATAGTTTAGAAAAGTTAGTTTACTGGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCAt < 1:322631/99‑1 (MQ=255) tttAAATCGTTTCC‑TTTTTGTACACCTCAAAAATTTAAAAGGTGTGTTTACTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCAt < 2:856659/99‑1 (MQ=255) tCAATTGTTCCC‑TTTTTTTAAAAATAATATATTTGAAAAGTGATTTTCTTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGa < 2:1019514/99‑1 (MQ=255) aaTCGTTCCC‑TTTTGCTACAACTAATAGATTTGAAAAGGGATTTTACTGGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGAtt < 2:925240/99‑1 (MQ=255) tttttttCC‑TTTTTGTACATCTCATAGTTTTAAAAGGTGTTTTTTCTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGAttt < 2:957773/95‑1 (MQ=255) ttCGTTTCC‑TTTTTATAAAACTAATAGATTTAAAAAGTGATTTTCTTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGAttt < 1:1231193/98‑1 (MQ=255) aTCTTTTCC‑TTTTTGTAAAACTAATAGATTTGAAAAGTGATTTTCCTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGAttt < 2:788485/99‑1 (MQ=255) aTCTTTTCC‑TCTTTGTACAACTCATAGTTTTAAAAAGTGTTTTTATTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGAttt < 2:1201458/99‑1 (MQ=255) tCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGAtttt > 1:1250486/1‑99 (MQ=255) cGTTCCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGATTTTCCTGGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTc < 2:675225/99‑1 (MQ=255) gTTTCC‑TTTTTGTACAACTAATAGTTTTAAAAAGGGAGTTTACTGGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCa < 2:893182/99‑1 (MQ=255) ttCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACg > 1:800235/1‑99 (MQ=255) ctttttttTACAACTCATATATTTAAAAAGTGAGTTTACTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCa < 2:1125128/95‑1 (MQ=255) tttttGCACAACTCATAATTTTAAAAAGTGATTTTACTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCaaa < 1:757230/97‑1 (MQ=255) tttttGAACAACTAATAGATTTAAAAAGGGTGTTTCTTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCaaa < 1:949604/97‑1 (MQ=255) tctTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCaaa > 1:878172/1‑99 (MQ=255) tttGTACAACTAATAGATTAAAAAAGTGATTTTTTTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACg < 1:1186450/99‑1 (MQ=255) ttGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGt > 2:200805/1‑99 (MQ=255) ttGTACAACTCAAAGATTTAAAAATTGTTTTTACTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGt < 2:949292/99‑1 (MQ=255) tGTACACCTCATAGATTTGAAAAGTGAGTTTACTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGtt < 2:1432612/99‑1 (MQ=255) tGTACAACTAATAGATTTGAAAAGTGAGTTTTCTTGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGtt < 2:579907/99‑1 (MQ=255) tGTACAACTAATAGATTTGAAAAGTGAGTTTACTTGACAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGtt < 2:378221/99‑1 (MQ=255) gTAAAACACAAAGTTTTGAAATGTGTTTTTGGGTGCCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGttt < 1:1641523/99‑1 (MQ=255) tACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTg > 2:584161/1‑99 (MQ=255) aaaaCTCATAGATTTGAAAAGTGAGTTTACTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGa < 1:62109/97‑1 (MQ=255) aCAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGa > 2:1218556/1‑99 (MQ=255) cAACTGATAGATTTGAAAAGTGAGTTTACTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGAt < 2:266330/99‑1 (MQ=255) aCTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATaa > 2:1381978/1‑99 (MQ=255) ctaaTAGATTTAAAAATTGATTTTCCTGGCAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATaaa < 1:271492/96‑1 (MQ=255) cTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACACTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATaaa > 2:4004/1‑99 (MQ=255) tCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAAt > 1:1156395/1‑99 (MQ=255) tCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAAt > 1:1260476/1‑99 (MQ=255) cATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCATACGTTTGATAAATg > 1:97300/1‑99 (MQ=255) aGATTTAAAAAGTGATTTTATTTGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAAt < 1:1511903/99‑1 (MQ=255) gATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAAtt > 1:264131/1‑99 (MQ=255) tGAAAAGTGATTTTACTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAAtttttt < 1:580418/99‑1 (MQ=255) gAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTc > 2:995359/1‑99 (MQ=255) gAAAAGTGAGTTTACTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTc < 2:713977/99‑1 (MQ=255) aaaaGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCt > 1:477968/1‑99 (MQ=255) aaaGTGATTTTACTGGAAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTg < 1:705600/99‑1 (MQ=255) tGAGTTTACTTGGAAAGCCATACACTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTa < 1:4004/99‑1 (MQ=255) attttACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTaga < 2:57956/97‑1 (MQ=255) gTTTACTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTagag < 1:273367/99‑1 (MQ=255) tttATTGGGAAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTAGAGt < 2:2593/99‑1 (MQ=255) tattGACAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTAGAGTtaa < 1:1124737/97‑1 (MQ=255) cTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTAGAGTtaat > 2:70421/1‑99 (MQ=255) gCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTAGAGTTAATAAAc > 2:173087/1‑99 (MQ=255) | TTTAACTTCTTCCACAAGTTGATAAGGATTTCCCTCAATTTTTTCAATCGTTTCC‑TCTTTGTACAACTCATAGATTTGAAAAGTGAGTTTACTTGGCAAGCCATACTCTGCAAGCTTGGTCAGCACTTTTTCCATACCATGATTTTCACGCAAACGTTTGATAAATGAATTTTTTCTGGCTAGAGTTAATAAAC > CP002094/1904527‑1904720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |