Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002094	305,497	A→G	V365V (GTT→GTC)	LLNZ_01670 ←	Nisin biosynthesis two‑component system, sensor histidine kinase NisK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002094	305,497	0	A	G	100.0%	269.6 / NA	65	V365V (GTT→GTC)	LLNZ_01670	Nisin biosynthesis two‑component system, sensor histidine kinase NisK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/29);  total (36/29)

TTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTAACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  CP002094/305403‑305591                                                                                               |                                                                                               ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  2:980752/99‑1 (MQ=255) ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  2:835113/99‑1 (MQ=255) ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  1:492589/99‑1 (MQ=255) ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  2:1269238/99‑1 (MQ=255)   ccAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtt                                                                                          <  1:61404/99‑1 (MQ=255)      gCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAACATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAttttt                                                                                       >  1:1583104/1‑99 (MQ=255)       cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      <  2:383956/99‑1 (MQ=255)       cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      >  2:1486616/1‑99 (MQ=255)       cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      <  2:1187625/99‑1 (MQ=255)       cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      <  1:1225932/99‑1 (MQ=255)        aTTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTc                                                                                     >  2:1121064/1‑99 (MQ=255)        aTTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTc                                                                                     >  2:1021577/1‑99 (MQ=255)         tttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCa                                                                                    <  2:783770/99‑1 (MQ=255)         tttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCa                                                                                    <  1:1580904/99‑1 (MQ=255)          ttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAc                                                                                   <  2:120212/99‑1 (MQ=255)          ttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAc                                                                                   <  1:980012/99‑1 (MQ=255)           tttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTT‑ATCATCATAAATACTCAAACTGACTATTTTTTCAccc                                                                                 >  1:810915/1‑99 (MQ=255)            ttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAccc                                                                                 >  1:624791/1‑99 (MQ=255)            ttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAccc                                                                                 <  2:1148212/99‑1 (MQ=255)                 tGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATTCTCAAACTGACTATTTTTTCTCCCTCttt                                                                            <  2:362106/99‑1 (MQ=255)                   cTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAg                                                                          <  1:462022/99‑1 (MQ=255)                    ttGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGc                                                                         <  2:80855/99‑1 (MQ=255)                        tCAGAAAAAAGATGACCCATTTTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATAc                                                                     <  1:1369853/99‑1 (MQ=255)                              aaaGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAg                                                               >  2:593265/1‑99 (MQ=255)                                aGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGc                                                             >  1:1225425/1‑99 (MQ=255)                                aGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGc                                                             >  1:986551/1‑99 (MQ=255)                                  gATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCAt                                                           >  1:728841/1‑99 (MQ=255)                                     gACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTaa                                                        <  2:1518665/99‑1 (MQ=255)                                      aCCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAc                                                       >  2:211435/1‑99 (MQ=255)                                      aCCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAc                                                       <  2:1318276/99‑1 (MQ=255)                                      aCCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAc                                                       >  1:271101/1‑99 (MQ=255)                                         attattCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACaaa                                                    >  1:791916/1‑99 (MQ=255)                                          ttattCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAg                                                   >  2:51034/1‑99 (MQ=255)                                             ttCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGata                                                >  1:456969/1‑99 (MQ=255)                                              tCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGatat                                               >  1:752520/1‑99 (MQ=255)                                                  gATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGAt                                           >  1:864857/1‑99 (MQ=255)                                                   aTTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATa                                          <  2:1634399/99‑1 (MQ=255)                                                    tttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATaa                                         >  2:964498/1‑99 (MQ=255)                                                     ttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAg                                        <  1:220349/99‑1 (MQ=255)                                                     ttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAg                                        <  2:483784/99‑1 (MQ=255)                                                     ttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAg                                        <  1:67641/99‑1 (MQ=255)                                                         aaaTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCt                                    <  2:1451568/99‑1 (MQ=255)                                                         aaaTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCt                                    >  1:637946/1‑99 (MQ=255)                                                          aaTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTc                                   >  2:1573438/1‑99 (MQ=255)                                                           aTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCg                                  >  2:1183795/1‑99 (MQ=255)                                                           aTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCg                                  >  2:1475497/1‑99 (MQ=255)                                                               agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              <  2:624791/99‑1 (MQ=255)                                                               agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              >  2:658270/1‑99 (MQ=255)                                                               agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              <  2:264886/99‑1 (MQ=255)                                                               agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              >  2:877733/1‑99 (MQ=255)                                                                gaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTa                             >  1:1410529/1‑99 (MQ=255)                                                                 atatTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTaa                            <  2:1115175/99‑1 (MQ=255)                                                                   atTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAg                          <  2:399117/99‑1 (MQ=255)                                                                    tttttCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGc                         >  1:1216059/1‑99 (MQ=255)                                                                    tttttCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGc                         >  1:573600/1‑99 (MQ=255)                                                                     ttttCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCt                        <  1:958256/99‑1 (MQ=255)                                                                        tcatcatcaTAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAAt                     >  1:1554757/1‑99 (MQ=255)                                                                           tcatcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTa                  >  2:567264/1‑99 (MQ=255)                                                                            catcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAt                 <  1:11577/99‑1 (MQ=255)                                                                             atcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAtt                <  2:311256/99‑1 (MQ=255)                                                                             atcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAtt                >  2:725843/1‑99 (MQ=255)                                                                              tcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAttt               <  2:553292/99‑1 (MQ=255)                                                                              tcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAttt               <  1:1256215/99‑1 (MQ=255)                                                                              tcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAttt               >  1:811505/1‑99 (MQ=255)                                                                                 tAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCg            >  1:401092/1‑99 (MQ=255)                                                                                   aaTACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTa          >  2:408874/1‑99 (MQ=255)                                                                                   aaTACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTa          >  2:57553/1‑99 (MQ=255)                                                                                   aaTACTCAAACTGACTATTTTTTCACCCTCTTTAACATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTa          >  2:130841/1‑99 (MQ=255)                                                                                       cTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTaaaaa      >  1:297329/1‑99 (MQ=255)                                                                                           aactgactATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTgg  >  1:608910/1‑99 (MQ=255)                                                                                           aactgactATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTgg  >  1:593837/1‑99 (MQ=255)                                                                                           aactgactATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTgg  >  2:633798/1‑99 (MQ=255)                                                                                               |                                                                                               TTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTAACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  CP002094/305403‑305591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑