BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002094	1,238,479	C→A	D34E (GAC→GAA)	LLNZ_06550 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002094	1,238,479	0	C	A	93.4%	322.4 / 12.9	91	D34E (GAC→GAA)	LLNZ_06550	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (40/45); total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AAAATCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATGGCG  >  CP002094/1238381‑1238573

aaaaTCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAc                                                                                                <  1:223614/99‑1 (MQ=255)

  aaTCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGa                                                                                              >  2:98658/1‑99 (MQ=255)

  aaTCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGa                                                                                              <  1:531190/99‑1 (MQ=255)

  aaTCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGa                                                                                              >  2:242991/1‑99 (MQ=255)

    tCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAgg                                                                                            >  2:958184/1‑99 (MQ=255)

     cATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAggc                                                                                           >  2:758146/1‑99 (MQ=255)

     cATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAggc                                                                                           >  2:1652567/1‑99 (MQ=255)

      aTTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAggcg                                                                                          >  2:801723/1‑99 (MQ=255)

         aaaGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCt                                                                                       >  2:294556/1‑99 (MQ=255)

         aaaGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCt                                                                                       >  2:327840/1‑99 (MQ=255)

          aaGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAggcggggg                                                                                      >  1:899583/1‑96 (MQ=255)

          aaGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTg                                                                                      >  2:645108/1‑99 (MQ=255)

              aGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGCACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGa                                                                                  >  2:721032/1‑99 (MQ=255)

                tCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGaaa                                                                                <  2:959564/99‑1 (MQ=255)

                  cATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACa                                                                              >  2:1566685/1‑99 (MQ=255)

                  cATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACa                                                                              >  2:1496789/1‑99 (MQ=255)

                   atatTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGAAACAg                                                                             >  1:606229/1‑99 (MQ=255)

                    tatTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCGGGTGAAACAGc                                                                            >  1:273853/1‑99 (MQ=255)

                      ttAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCaa                                                                          >  1:103585/1‑99 (MQ=255)

                      ttAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCaa                                                                          >  2:571574/1‑99 (MQ=255)

                       tAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCaaa                                                                         <  2:824753/99‑1 (MQ=255)

                           ggAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGAAACAGCAAATGGc                                                                     <  2:1214531/99‑1 (MQ=255)

                           ggAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGc                                                                     >  2:446773/1‑99 (MQ=255)

                           ggAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGc                                                                     <  1:439751/99‑1 (MQ=255)

                            gAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCCGTTGAAACAGCAAAAgggc                                                                    >  1:185038/1‑97 (MQ=255)

                             aaCTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGccc                                                                   <  1:1223031/99‑1 (MQ=255)

                              aCTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGcccc                                                                  >  2:1472917/1‑98 (MQ=255)

                                 gACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGGTGGAAAAGCAAAAGGgccccca                                                               >  1:182216/1‑95 (MQ=255)

                                     aacgtaaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAagag                                                           <  2:59159/99‑1 (MQ=255)

                                     aacgtaaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAagag                                                           >  2:566583/1‑99 (MQ=255)

                                      acgtaaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCCAATGGCCCACAAagagc                                                          >  2:1175152/1‑98 (MQ=255)

                                      acgtaaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGt                                                          <  1:271891/99‑1 (MQ=255)

                                       cgtaaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGtt                                                         <  1:549634/99‑1 (MQ=255)

                                       cgtaaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGtt                                                         <  2:377838/99‑1 (MQ=255)

                                         taaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGa                                                       >  2:683996/1‑99 (MQ=255)

                                          aaacgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGaa                                                      >  2:185370/1‑99 (MQ=255)

                                            acgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAAt                                                    <  2:534308/99‑1 (MQ=255)

                                             cgtTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATc                                                   >  2:1014656/1‑99 (MQ=255)

                                               ttACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGGAACAGCAAATGGCCCACAAAGAGTTGAAATCGt                                                 >  2:1310925/1‑99 (MQ=255)

                                               ttACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGGAAAAGCAAAAAGCCCACAAAGAGGTGGAAACGt                                                 >  1:110331/1‑99 (MQ=255)

                                               ttACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCgg                                                 >  2:911077/1‑98 (MQ=255)

                                               ttACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATAGCCCACAAAGAGTTGAAATCgg                                                 >  2:721565/1‑98 (MQ=255)

                                                tACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGtt                                                <  1:758146/99‑1 (MQ=255)

                                                tACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGtt                                                <  1:308655/99‑1 (MQ=255)

                                                 aCTGGAAGGTACCAGACCACCTACAACCAATTAAACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTg                                               <  1:164728/99‑1 (MQ=255)

                                                  cTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGa                                              <  2:635023/99‑1 (MQ=255)

                                                    ggAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATg                                            <  2:688197/99‑1 (MQ=255)

                                                     gAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGt                                           <  1:737287/99‑1 (MQ=255)

                                                      aaGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTc                                          >  2:376155/1‑99 (MQ=255)

                                                       cagTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCGAATGGCCCACAAAGAGTTGAAATCGTTGATGTCg                                         <  2:1355375/97‑1 (MQ=255)

                                                        ggTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGt                                        >  2:56511/1‑99 (MQ=255)

                                                          tACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTaa                                      >  2:1445470/1‑99 (MQ=255)

                                                            ccAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAAcc                                    >  1:945961/1‑99 (MQ=255)

                                                              aGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTc                                  <  1:1107638/99‑1 (MQ=255)

                                                              aGACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTc                                  <  1:327840/99‑1 (MQ=255)

                                                               gacgacCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAAAAAATGGCCCACAAAGAGGTGAAATCGTTGATGTCGTAACCTCa                                 >  2:871880/1‑99 (MQ=255)

                                                               gACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGGAACAGCAAATGGCCCACAAAAAATTGAAAGCATTGATTTCGTAACCCCa                                 >  1:276355/1‑99 (MQ=255)

                                                               gACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCa                                 <  2:94250/99‑1 (MQ=255)

                                                               gACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCa                                 >  2:669746/1‑99 (MQ=255)

                                                               gACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCa                                 <  1:245946/99‑1 (MQ=255)

                                                               gACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCa                                 <  2:318803/99‑1 (MQ=255)

                                                               gACCACCTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCa                                 <  2:1350884/99‑1 (MQ=255)

                                                                accaccTACAACCAATTAGACTATGTAAGGGTGAAGAGGCGGCTGTTGAAAAAGAAAATGGCCCCCAAAAGGTGGAAATGGTTGATTCGGTAAcccccg                                >  1:11023/1‑95 (MQ=255)

                                                                accaccTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCAc                                <  2:938764/99‑1 (MQ=255)

                                                                accaccTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCAc                                >  2:1000519/1‑99 (MQ=255)

                                                                 ccaccTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACg                               <  1:1636249/99‑1 (MQ=255)

                                                                 ccaccTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACg                               >  2:702909/1‑99 (MQ=255)

                                                                 ccaccTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACg                               <  1:1397117/99‑1 (MQ=255)

                                                                 ccaccTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACg                               <  2:1508027/99‑1 (MQ=255)

                                                                  caccTACAACCAATTAGACTACGTAAGGGTGAAGACGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGt                              <  1:790367/99‑1 (MQ=255)

                                                                   accTACAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTg                             <  2:1407346/99‑1 (MQ=255)

                                                                       aCAACCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCACATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATgg                         <  1:360873/99‑1 (MQ=255)

                                                                         aaCCAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGt                       <  2:1567525/99‑1 (MQ=255)

                                                                            cAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGtttt                    <  1:1567439/99‑1 (MQ=255)

                                                                            cAATTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGtttt                    <  1:1083187/99‑1 (MQ=255)

                                                                              aTTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGtttttt                  >  2:1239123/1‑98 (MQ=255)

                                                                              aTTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTta                  <  1:727121/99‑1 (MQ=255)

                                                                              aTTAGACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTta                  <  1:1277250/99‑1 (MQ=255)

                                                                                 aGACCACGTAAGGGTGAAGAGGCGGCTGGTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTatat               <  1:841149/99‑1 (MQ=255)

                                                                                  gACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATAtt              <  1:170244/99‑1 (MQ=255)

                                                                                  gACTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATAtt              <  1:99475/99‑1 (MQ=255)

                                                                                    cTACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCCCAAAGAGTTGAAATCGTTGATGTCGTAACCCCACGTGATGGGGTTTTATATTgg            >  1:8018/1‑99 (MQ=255)

                                                                                     tACGTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTTTATTTGa           >  1:1573606/1‑99 (MQ=255)

                                                                                        gTAAGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGaaaa        <  2:90161/99‑1 (MQ=255)

                                                                                          aaGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAAt      <  1:117665/99‑1 (MQ=255)

                                                                                          aaGGGTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAAt      <  2:845304/99‑1 (MQ=255)

                                                                                            gggTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATgg    <  1:1024083/99‑1 (MQ=255)

                                                                                            gggTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATgg    >  2:1109500/1‑99 (MQ=255)

                                                                                            gggTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATgg    <  2:1149247/99‑1 (MQ=255)

                                                                                             ggTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATGGc   <  1:840105/99‑1 (MQ=255)

                                                                                             ggTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATGGc   >  2:532318/1‑99 (MQ=255)

                                                                                              gTGAAGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATGGCg  <  2:182216/99‑1 (MQ=255)

AAAATCATTAAAGCAGTCCATATTAATGGAACTGACAAACGTAAACGTTACTGGAAGGTACCAGACCACCTACAACCAATTAGACTACGTAAGGGTGACGAGGCGGCTGTTGAAACAGCAAATGGCCCACAAAGAGTTGAAATCGTTGATGTCGTAACCTCACGTGATGGGTTTTTATATTGGAAAAATGGCG  >  CP002094/1238381‑1238573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`