Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002094	305,693	C→A	R300I (AGA→ATA)	LLNZ_01670 ←	Nisin biosynthesis two‑component system, sensor histidine kinase NisK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002094	305,693	0	C	A	100.0%	330.6 / NA	84	R300I (AGA→ATA)	LLNZ_01670	Nisin biosynthesis two‑component system, sensor histidine kinase NisK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/45);  total (39/45)

GTTTTTTTAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTCTCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTTAAACTATTTTTCATTGA  >  CP002094/305600‑305787                                                                                              |                                                                                               gTTTTTTTAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCt                                                                                           <  2:1652853/99‑1 (MQ=255)  tttttttAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCtt                                                                                          >  2:1051266/1‑99 (MQ=255)  tttttttAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCtt                                                                                          >  2:1443820/1‑99 (MQ=255)   ttttttAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCttt                                                                                         >  1:172969/1‑99 (MQ=255)   ttttttAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCttt                                                                                         >  1:286543/1‑99 (MQ=255)   ttttttAACTAGCTGATAATCCACTTGATAAGTTGCTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGTAGATTATCGCttt                                                                                         >  2:1351280/1‑99 (MQ=255)    tttttAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTg                                                                                        <  2:1410529/99‑1 (MQ=255)    tttttAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTg                                                                                        >  1:1636672/1‑99 (MQ=255)        tAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAAt                                                                                    <  2:1225425/99‑1 (MQ=255)          aCTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCa                                                                                  >  2:1063959/1‑99 (MQ=255)          aCTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCa                                                                                  <  2:735097/99‑1 (MQ=255)          aCTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCa                                                                                  <  2:137062/99‑1 (MQ=255)           cTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCAt                                                                                 >  1:782240/1‑99 (MQ=255)            tAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCAtt                                                                                >  1:592975/1‑99 (MQ=255)             aGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCAttt                                                                               <  1:1021577/99‑1 (MQ=255)             aGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCAttt                                                                               <  2:757199/99‑1 (MQ=255)                   tAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTtcatca                                                                         <  2:190075/99‑1 (MQ=255)                   tAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTtcatca                                                                         >  1:975515/1‑99 (MQ=255)                   tAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTtcatca                                                                         <  2:1323983/99‑1 (MQ=255)                    aaTCCATTTAATAAGATGTTGACATTATCTACAACAAAACTGATTAATATAACAAAAAATCCTCCAGGTAGATTATCTCTTTGTAATCATTTTcatcat                                                                        <  2:401092/99‑1 (MQ=255)                     aTCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCAtt                                                                       <  2:256799/99‑1 (MQ=255)                     aTCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCAtt                                                                       <  2:107167/99‑1 (MQ=255)                      tCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTc                                                                      >  1:646655/1‑99 (MQ=255)                       ccACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCa                                                                     >  2:135104/1‑99 (MQ=255)                         gcTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCaaa                                                                   <  2:548024/98‑1 (MQ=255)                         aCTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCaaa                                                                   >  2:165832/1‑99 (MQ=255)                             gATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGtt                                                               >  1:65360/1‑99 (MQ=255)                             gATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGtt                                                               <  2:825524/99‑1 (MQ=255)                               tAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGtttt                                                             >  1:878433/1‑99 (MQ=255)                               tAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGtttt                                                             <  2:1272240/99‑1 (MQ=255)                                aaGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTg                                                            >  1:579404/1‑99 (MQ=255)                                  gttgttGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGta                                                          <  1:57553/99‑1 (MQ=255)                                   ttgttgACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGtat                                                         >  2:987422/1‑99 (MQ=255)                                      ttgACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAAc                                                      >  1:216831/1‑99 (MQ=255)                                      ttgACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAAc                                                      <  1:559519/99‑1 (MQ=255)                                         aCAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTaa                                                   <  2:297329/99‑1 (MQ=255)                                          cAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAAt                                                  >  2:591084/1‑99 (MQ=255)                                          cAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAAt                                                  <  1:84964/99‑1 (MQ=255)                                            aCTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCa                                                >  2:898181/1‑99 (MQ=255)                                             ctctTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCAt                                               <  1:112746/99‑1 (MQ=255)                                             ctctTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCAt                                               <  1:899060/99‑1 (MQ=255)                                               ctTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATtg                                             <  1:1183795/99‑1 (MQ=255)                                               ctTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATtg                                             >  1:862411/1‑99 (MQ=255)                                                 tCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATtgtg                                           <  1:1285049/99‑1 (MQ=255)                                                  ccAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTgtgt                                          <  2:892621/99‑1 (MQ=255)                                                     actcaactGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTaa                                       <  1:40811/99‑1 (MQ=255)                                                          actGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATAc                                  <  2:728841/99‑1 (MQ=255)                                                          actGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATAc                                  <  1:474374/99‑1 (MQ=255)                                                          actGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATAc                                  >  2:1020416/1‑99 (MQ=255)                                                          actGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATAc                                  >  1:1631383/1‑99 (MQ=255)                                                          actGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATAc                                  >  1:479525/1‑99 (MQ=255)                                                           ctGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTa                                        >  2:299980/1‑92 (MQ=255)                                                           ctGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTa                                        <  1:299980/92‑1 (MQ=255)                                                           ctGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACt                                 <  1:717867/99‑1 (MQ=255)                                                           ctGATAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACt                                 <  2:115647/99‑1 (MQ=255)                                                            tGATAAAACTATCAAAAAATCCTCCAGGGAGATTAGCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTACAAGACtt                                <  2:515116/99‑1 (MQ=255)                                                               tAAATCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTc                             >  2:1625361/1‑99 (MQ=255)                                                                 aaTCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCaa                           >  1:597834/1‑99 (MQ=255)                                                                  atcaatcaAAAAATCCTCCAGGGTTATTATCTCTTTGTAATCATTTTCATCATTCAAATGTTTTTTATAACTAATCATTGTTTTAAAATACTTGTCaaa                          <  2:158886/99‑1 (MQ=255)                                                                   tCTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCaaaa                         >  1:951193/1‑99 (MQ=255)                                                                    cTATCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCaaaaa                        >  2:191035/1‑99 (MQ=255)                                                                      aTCAAAAAATCCTCCAGGGAGATTATTGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACa                      <  1:1400846/99‑1 (MQ=255)                                                                      aTCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACa                      >  2:994104/1‑99 (MQ=255)                                                                       tCAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAg                     <  2:1503484/99‑1 (MQ=255)                                                                       tCAAAAAAGCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAg                     <  1:1166429/99‑1 (MQ=255)                                                                        cAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGt                    <  1:211435/99‑1 (MQ=255)                                                                        cAAAAAATCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGt                    <  2:545753/99‑1 (MQ=255)                                                                          aaaaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGtca                  >  2:773023/1‑97 (MQ=255)                                                                           aaaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCaa                 >  2:1488276/1‑99 (MQ=255)                                                                            aaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCaaa                <  1:1475497/99‑1 (MQ=255)                                                                            aaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCaaa                <  2:752520/99‑1 (MQ=255)                                                                               tcctccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTa             <  1:964498/99‑1 (MQ=255)                                                                               tcctccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTa             <  2:1597538/99‑1 (MQ=255)                                                                                 ctccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAtt           <  1:577766/99‑1 (MQ=255)                                                                                  tccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAttt          <  2:364922/99‑1 (MQ=255)                                                                                  tccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAttt          >  1:1274204/1‑99 (MQ=255)                                                                                    cAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAttttt        >  2:1264443/1‑99 (MQ=255)                                                                                      gggAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCa      >  2:1505725/1‑99 (MQ=255)                                                                                      gggAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCa      >  2:24643/1‑99 (MQ=255)                                                                                        gagaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCAtt    >  2:23717/1‑99 (MQ=255)                                                                                        gagaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCAtt    >  1:1603222/1‑99 (MQ=255)                                                                                        gagaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCAtt    <  2:1251334/99‑1 (MQ=255)                                                                                          gaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGa  >  1:154310/1‑99 (MQ=255)                                                                                          gaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGa  <  2:1554757/99‑1 (MQ=255)                                                                                              |                                                                                               GTTTTTTTAACTAGCTGATAATCCACTTGATAAGTTGTTGACAACTCTTCCAACTCAACTGATAAATCTATCAAAAAATCCTCCAGGGAGATTCTCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTTAAACTATTTTTCATTGA  >  CP002094/305600‑305787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑