Predicted mutation
evidence seq id position mutation annotation gene description
RA CP002094 305,770 T→C L274L (TTA→TTG)  LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP002094305,7700TC99.0% 424.3 / NA 104L274L (TTA→TTG) LLNZ_01670Nisin biosynthesis two‑component system, sensor histidine kinase NisK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (54/49);  minor base A (0/1);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

AAAATCCTCCAGGGAGATTCTCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTTAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGG  >  CP002094/305674‑305865
                                                                                                |                                                                                               
aaaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCaa                                                                                               >  2:1488276/1‑99 (MQ=255)
 aaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCaaa                                                                                              <  1:1475497/99‑1 (MQ=255)
 aaaTCCTCCAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCaaa                                                                                              <  2:752520/99‑1 (MQ=255)
    tcctccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTa                                                                                           <  1:964498/99‑1 (MQ=255)
    tcctccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTa                                                                                           <  2:1597538/99‑1 (MQ=255)
      ctccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAtt                                                                                         <  1:577766/99‑1 (MQ=255)
       tccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAttt                                                                                        <  2:364922/99‑1 (MQ=255)
       tccAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAttt                                                                                        >  1:1274204/1‑99 (MQ=255)
         cAGGGAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTAttttt                                                                                      >  2:1264443/1‑99 (MQ=255)
           gggAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCa                                                                                    >  2:1505725/1‑99 (MQ=255)
           gggAGATTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCa                                                                                    >  2:24643/1‑99 (MQ=255)
             gagaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCAtt                                                                                  <  2:1251334/99‑1 (MQ=255)
             gagaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCAtt                                                                                  >  2:23717/1‑99 (MQ=255)
             gagaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCAtt                                                                                  >  1:1603222/1‑99 (MQ=255)
               gaTTATCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGa                                                                                <  2:1554757/99‑1 (MQ=255)
                  tatcGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTc                                                                             <  2:172969/97‑1 (MQ=255)
                   atcGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCa                                                                            <  2:502187/98‑1 (MQ=255)
                    tcGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCaa                                                                           <  1:119450/99‑1 (MQ=255)
                      gCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATa                                                                         >  1:1539156/1‑99 (MQ=255)
                      gCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATa                                                                         >  2:1148191/1‑99 (MQ=255)
                      gCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATa                                                                         <  2:864857/99‑1 (MQ=255)
                       cTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCATAAAGAGTCAAACTATTTTTCATTGACTCAATaa                                                                        >  1:780367/1‑99 (MQ=255)
                       cTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATaa                                                                        >  1:1110789/1‑99 (MQ=255)
                          tGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAAt                                                                     >  1:1326431/1‑99 (MQ=255)
                          tGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAAt                                                                     <  2:862411/99‑1 (MQ=255)
                             aaTCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAg                                                                  <  1:1186658/99‑1 (MQ=255)
                              aTCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGc                                                                 >  2:308405/1‑99 (MQ=255)
                               tCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCt                                                                >  2:1618358/1‑99 (MQ=255)
                               tCATTTTAATCATTTAAAAATTTTATATAACTAATCATTGTGTTAAAATAATTAACAAAAACATTCAATTTTTTTTTCATTGACTCAATAAAATCAGCt                                                                <  1:1618358/99‑1 (MQ=255)
                                cATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCtt                                                               >  2:1634284/1‑99 (MQ=255)
                                 aTTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCttg                                                              <  1:725843/99‑1 (MQ=255)
                                  ttttCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCttgt                                                             <  1:1063959/99‑1 (MQ=255)
                                  ttttCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCttgt                                                             <  1:1121064/99‑1 (MQ=255)
                                   tttCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCttgtt                                                            <  1:400743/99‑1 (MQ=255)
                                   tttCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCttgtt                                                            >  1:766820/1‑99 (MQ=255)
                                     tcatcaATCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTtgttgt                                                          <  2:592975/99‑1 (MQ=255)
                                      catcatTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTgttgtt                                                         >  2:775204/1‑99 (MQ=255)
                                        tcatTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCa                                                       <  1:1526797/99‑1 (MQ=255)
                                        tcatTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCa                                                       >  2:1155830/1‑99 (MQ=255)
                                          atTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCAtt                                                     <  2:154310/99‑1 (MQ=255)
                                          atTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCAtt                                                     <  2:456969/99‑1 (MQ=255)
                                           ttCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCAttt                                                    <  1:877733/99‑1 (MQ=255)
                                           ttCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCAttt                                                    >  1:868080/1‑99 (MQ=255)
                                              aaaaGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACt                                                 >  1:82265/1‑99 (MQ=255)
                                               aaaGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACtt                                                >  2:920814/1‑99 (MQ=255)
                                               aaaGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACtt                                                >  2:1658728/1‑99 (MQ=255)
                                                aaGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTc                                               >  2:860760/1‑99 (MQ=255)
                                                aaGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTc                                               >  1:1147010/1‑99 (MQ=255)
                                                  gTTTTGTATATCTAATCATTGTGTTAAAATACTAGTCATAAAAAGTAAAATTATTATTCATTGGATTAATAAAATCATCTTTTAGTTCATTTACTACAg                                             <  2:73977/99‑1 (MQ=255)
                                                  gTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAg                                             <  2:811505/99‑1 (MQ=255)
                                                  gTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAg                                             <  1:1371242/99‑1 (MQ=255)
                                                  gTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAg                                             >  1:490261/1‑99 (MQ=255)
                                                   ttttGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGt                                            <  1:1408733/99‑1 (MQ=255)
                                                   ttttGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGt                                            <  1:1443820/99‑1 (MQ=255)
                                                   tttaGTAGAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCCGt                                            >  2:1363552/1‑99 (MQ=255)
                                                     ttGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCa                                          >  2:495290/1‑99 (MQ=255)
                                                      tGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCAt                                         <  1:1020416/99‑1 (MQ=255)
                                                      tGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCAt                                         >  1:1071570/1‑99 (MQ=255)
                                                       gTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCAtc                                        >  2:330669/1‑99 (MQ=255)
                                                          caaCTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATctct                                     >  1:540650/2‑99 (MQ=255)
                                                           aaCTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTa                                    >  2:1141371/1‑99 (MQ=255)
                                                            aCTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAg                                   <  2:1636672/99‑1 (MQ=255)
                                                            aCTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAg                                   >  2:34193/1‑99 (MQ=255)
                                                            aCTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAg                                   <  1:290551/99‑1 (MQ=255)
                                                             cTAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGc                                  >  1:179897/1‑99 (MQ=255)
                                                              tAATCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCa                                 <  2:727634/99‑1 (MQ=255)
                                                               aaTCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAg                                <  1:730627/99‑1 (MQ=255)
                                                                aTCATTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGt                               >  1:822619/1‑99 (MQ=255)
                                                                   aTTGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCa                            >  1:37790/1‑99 (MQ=255)
                                                                    ttGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCaa                           <  2:975515/99‑1 (MQ=255)
                                                                    ttGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCaa                           >  1:1525809/1‑99 (MQ=255)
                                                                    ttGTGTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCaa                           >  1:1545034/1‑99 (MQ=255)
                                                                     tgtgTTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAat                          >  1:1182196/1‑99 (MQ=255)
                                                                      gtgtTAAAATACTTGTCAAAAACAGTCAAACTGTTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAata                         <  1:692547/99‑1 (MQ=255)
                                                                       tgtTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAatat                        <  1:1511569/99‑1 (MQ=255)
                                                                       tgtTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAatat                        <  1:1366725/99‑1 (MQ=255)
                                                                        gtTAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATAtt                       >  1:1531809/1‑99 (MQ=255)
                                                                         ttAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATAttt                      >  2:344022/1‑99 (MQ=255)
                                                                         ttAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATAttt                      <  2:66792/99‑1 (MQ=255)
                                                                          tAAAATACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTc                     <  2:878433/99‑1 (MQ=255)
                                                                            aaaTACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCt                   <  1:135104/99‑1 (MQ=255)
                                                                            aaaTACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCt                   >  2:826971/1‑99 (MQ=255)
                                                                            aaaTACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCt                   <  1:1555411/99‑1 (MQ=255)
                                                                            aaaTACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCt                   >  2:1063210/1‑99 (MQ=255)
                                                                             aaTACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCtt                  <  2:593837/99‑1 (MQ=255)
                                                                              aTACTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCttc                 >  1:573663/1‑98 (MQ=255)
                                                                                 cTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTaa              <  2:65360/99‑1 (MQ=255)
                                                                                 cTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTaa              <  2:694502/99‑1 (MQ=255)
                                                                                 cTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTaa              >  1:450077/1‑99 (MQ=255)
                                                                                 cTTGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTaa              >  1:1396478/1‑99 (MQ=255)
                                                                                   tGTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATa            >  2:19571/1‑99 (MQ=255)
                                                                                    gTCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATAc           <  1:987422/99‑1 (MQ=255)
                                                                                     tCAAAAACAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACt          <  1:966352/99‑1 (MQ=255)
                                                                                       aaaaaCAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGt        <  1:350024/99‑1 (MQ=255)
                                                                                        aaaaCAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGtt       >  1:1382963/1‑99 (MQ=255)
                                                                                          aaCAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTaa     >  2:1181969/1‑99 (MQ=255)
                                                                                           aCAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTaaa    >  2:1315970/1‑99 (MQ=255)
                                                                                            cAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAg   >  1:1572659/1‑99 (MQ=255)
                                                                                            cAGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAg   >  1:451764/1‑99 (MQ=255)
                                                                                             aGTCAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAgg  >  1:1305704/1‑99 (MQ=255)
                                                                                                |                                                                                               
AAAATCCTCCAGGGAGATTCTCGCTTTGTAATCATTTTCATCATTCAAAAGTTTTGTATAACTAATCATTGTGTTAAAATACTTGTCAAAAACAGTTAAACTATTTTTCATTGACTCAATAAAATCAGCTTGTTGTTCATTTACTTCAGTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGG  >  CP002094/305674‑305865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑