BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002094	306,199	T→A	P131P (CCA→CCT)	LLNZ_01670 ←	Nisin biosynthesis two‑component system, sensor histidine kinase NisK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002094	306,199	0	T	A	100.0%	449.5 / NA	111	P131P (CCA→CCT)	LLNZ_01670	Nisin biosynthesis two‑component system, sensor histidine kinase NisK
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (57/54); total (57/54)

Only 100 of 111 total aligned reads displayed.

GACAGAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCTGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTGTTT  >  CP002094/306103‑306294

gACAGAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAgg                                                                                               >  1:838971/1‑99 (MQ=255)

 aCAGAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGc                                                                                              >  2:1630956/1‑99 (MQ=255)

  cAGAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGATGCGTATGATTTGTAAATTCAGGCa                                                                                             >  1:508015/1‑99 (MQ=255)

  cAGAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCa                                                                                             >  1:1526673/1‑99 (MQ=255)

    gAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATa                                                                                           >  1:950972/1‑99 (MQ=255)

     aaaaaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATAc                                                                                          >  1:1593528/1‑99 (MQ=255)

     aaaaaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATAc                                                                                          <  1:856403/99‑1 (MQ=255)

     aaaaaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATAc                                                                                          <  2:157572/99‑1 (MQ=255)

      aaaaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACt                                                                                         >  1:214053/1‑99 (MQ=255)

      aaaaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGAAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATCCt                                                                                         >  1:1141217/1‑99 (MQ=255)

       aaaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTg                                                                                        >  1:1077655/1‑99 (MQ=255)

        aaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGt                                                                                       <  1:861187/99‑1 (MQ=255)

        aaaTAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGt                                                                                       <  1:99418/99‑1 (MQ=255)

          ataatGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAGATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGttt                                                                                     >  1:209122/1‑99 (MQ=255)

          ataatGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGttt                                                                                     >  1:565713/1‑99 (MQ=255)

          ataatGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGttt                                                                                     <  1:936051/99‑1 (MQ=255)

            aatGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTg                                                                                   <  1:1220088/99‑1 (MQ=255)

             atGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGt                                                                                  <  2:1382730/99‑1 (MQ=255)

             atGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGt                                                                                  >  2:1149005/1‑99 (MQ=255)

              tGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTc                                                                                 >  2:199050/1‑99 (MQ=255)

              tGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTc                                                                                 >  2:732068/1‑99 (MQ=255)

               gAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCt                                                                                <  2:748895/99‑1 (MQ=255)

                   attattTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTaaa                                                                            <  2:1182812/99‑1 (MQ=255)

                    ttattTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTaaaa                                                                           <  1:170247/99‑1 (MQ=255)

                    ttattTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTaaaa                                                                           >  2:956181/1‑99 (MQ=255)

                    ttattTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTaaaa                                                                           >  1:112357/1‑99 (MQ=255)

                    ttattTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTaaaa                                                                           <  2:1350640/99‑1 (MQ=255)

                      attTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAaaca                                                                         >  1:947977/1‑99 (MQ=255)

                      attTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAaaca                                                                         <  2:1309807/99‑1 (MQ=255)

                       tttCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAaacaa                                                                        >  1:1172389/1‑99 (MQ=255)

                       tttCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAaacaa                                                                        >  2:27407/1‑99 (MQ=255)

                         tCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACt                                                                      <  1:1569380/99‑1 (MQ=255)

                          cACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTg                                                                     <  2:448984/99‑1 (MQ=255)

                           aCCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGa                                                                    >  2:1398585/1‑99 (MQ=255)

                              aagaaaaaagaaaaGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGaaaa                                                                 >  1:97344/1‑99 (MQ=255)

                                gaaaaaagaaaaGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAAt                                                               <  1:84372/99‑1 (MQ=255)

                                gaaaaaagaaaaGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAAt                                                               <  1:1351818/99‑1 (MQ=255)

                                 aaaaaagaaaaGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATg                                                              >  1:270198/1‑99 (MQ=255)

                                 aaaaaagaaaaGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATg                                                              <  2:52065/99‑1 (MQ=255)

                                  aaaaagaaaaGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGc                                                             <  1:1423478/99‑1 (MQ=255)

                                   aaaagaaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCt                                                            >  2:1231936/1‑99 (MQ=255)

                                     aagaaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTa                                                          >  2:886870/1‑99 (MQ=255)

                                     aagaaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTa                                                          >  1:867009/1‑99 (MQ=255)

                                     aagaaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTa                                                          >  2:213824/1‑99 (MQ=255)

                                      agaaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTAt                                                         <  2:1470649/99‑1 (MQ=255)

                                      agaaaagGTAAGTAAAATGATTATAAGAAAATTTACTAAGCGTATGATTTATAAATTCAGTCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTAt                                                         <  2:1172115/99‑1 (MQ=255)

                                       gaaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTAtt                                                        >  2:325773/1‑99 (MQ=255)

                                        aaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTg                                                       <  1:603194/99‑1 (MQ=255)

                                        aaaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTg                                                       <  2:867009/99‑1 (MQ=255)

                                         aaagGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGc                                                      <  1:636316/99‑1 (MQ=255)

                                           agGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCtt                                                    <  2:1423362/99‑1 (MQ=255)

                                             gtaagtaaATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGa                                                  >  1:1389965/1‑99 (MQ=255)

                                              taagtaaATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGaa                                                 <  1:956181/99‑1 (MQ=255)

                                              taagtaaATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGaa                                                 <  2:1027900/99‑1 (MQ=255)

                                                    aaTTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagta                                           >  2:1588594/1‑99 (MQ=255)

                                                    aaTTGATTATAAAAATTTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagta                                           <  1:549609/99‑1 (MQ=255)

                                                     aTTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGActaagtat                                          >  1:182736/1‑99 (MQ=255)

                                                     aTTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagtat                                          >  2:116595/1‑99 (MQ=255)

                                                      ttGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagtata                                         >  1:641218/1‑99 (MQ=255)

                                                        gATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagtataag                                       <  2:614341/99‑1 (MQ=255)

                                                        gATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagtataag                                       <  2:120959/99‑1 (MQ=255)

                                                         aTTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATTCTTATTGCTTGAataagtataagt                                      <  2:1471149/99‑1 (MQ=255)

                                                         aTTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagtataagt                                      >  1:735929/1‑99 (MQ=255)

                                                         aTTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAataagtataagt                                      <  2:956850/99‑1 (MQ=255)

                                                          ttATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAAtaagtataagta                                     >  2:568132/1‑99 (MQ=255)

                                                            ataAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAAt                                   >  2:31407/1‑99 (MQ=255)

                                                            ataAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAAt                                   >  2:100346/1‑99 (MQ=255)

                                                             taAGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAAtt                                  <  1:1657929/99‑1 (MQ=255)

                                                               aGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTtc                                >  2:847009/1‑99 (MQ=255)

                                                               aGAAATTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTtc                                >  2:197176/1‑99 (MQ=255)

                                                                 aaaTTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTtctc                              <  2:1034175/99‑1 (MQ=255)

                                                                  aaTTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTcc                             >  2:1230499/1‑99 (MQ=255)

                                                                   aTTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAGCAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCt                            >  1:783602/1‑99 (MQ=255)

                                                                   aTTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCt                            <  1:924536/99‑1 (MQ=255)

                                                                   aTTGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCt                            >  1:1023909/1‑99 (MQ=255)

                                                                    ttGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCtt                           <  1:1634284/99‑1 (MQ=255)

                                                                    ttGAACGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCtt                           >  2:1379778/1‑99 (MQ=255)

                                                                        aCGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGt                       >  2:1058634/1‑99 (MQ=255)

                                                                        aCGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGt                       >  2:811832/1‑99 (MQ=255)

                                                                         cGAAGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTa                      >  1:552538/1‑99 (MQ=255)

                                                                           aaGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAat                    >  2:1659756/1‑99 (MQ=255)

                                                                            aGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAata                   >  2:878837/1‑99 (MQ=255)

                                                                            aGCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAata                   <  1:654363/99‑1 (MQ=255)

                                                                             gCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAatat                  >  1:1353892/1‑99 (MQ=255)

                                                                             gCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAatat                  <  1:335363/99‑1 (MQ=255)

                                                                             gCGTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAatat                  <  2:702704/99‑1 (MQ=255)

                                                                               gTATGATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAAtatta                <  1:1469449/99‑1 (MQ=255)

                                                                                   gATTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTAtgtg            <  1:1476937/99‑1 (MQ=255)

                                                                                    aTTTGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGa           <  1:151639/99‑1 (MQ=255)

                                                                                       tGTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGa        <  1:253211/99‑1 (MQ=255)

                                                                                        gTAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGAc       <  2:470022/99‑1 (MQ=255)

                                                                                         tAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACt      >  1:962586/1‑99 (MQ=255)

                                                                                         tAAATTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACt      >  1:390437/1‑99 (MQ=255)

                                                                                         tAAATTCAGGCAAACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACt      >  1:766727/1‑99 (MQ=255)

                                                                                          aaaTTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTg     <  2:553526/99‑1 (MQ=255)

                                                                                          aaaTTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTg     <  2:920786/99‑1 (MQ=255)

                                                                                          aaaTTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTg     <  2:950972/99‑1 (MQ=255)

                                                                                           aaTTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTGt    >  1:1346346/1‑99 (MQ=255)

                                                                                            aTTCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTGtt   <  2:1077655/99‑1 (MQ=255)

                                                                                             ttCAGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTGttt  <  1:1148930/99‑1 (MQ=255)

GACAGAAAAAATAATGAGTATTATTTCACCAAGAAAAAAGAAAAGGTAAGTAAATTGATTATAAGAAATTGAACGAAGCGTATGATTTGTAAATTCTGGCATACTGTTTTGTCTTAAAACAACTGAAAAATGCTTATTGCTTGAATAAGTATAAGTAATTTCTCCTTCTGTAATATTATGTGAAGACTGTTT  >  CP002094/306103‑306294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`