| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP002094 | 306,367 | C→A | M75I (ATG→ATT) | LLNZ_01670 ← | Nisin biosynthesis two‑component system, sensor histidine kinase NisK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP002094 | 306,367 | 0 | C | A | 98.9% | 360.1 / NA | 89 | M75I (ATG→ATT) | LLNZ_01670 | Nisin biosynthesis two‑component system, sensor histidine kinase NisK |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (35/53); minor base G (1/0); total (36/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTAATATTATGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAACATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGTTTCT > CP002094/306271‑306457 | tGTAATATTATGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAat > 2:154010/1‑99 (MQ=255) tAATATTATGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataa < 2:1136788/99‑1 (MQ=255) aaTTTTATGTGAAGACTTTTTTAAAGCTAAAAAACTGGTTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAaataat < 1:770500/99‑1 (MQ=255) aaTATTATGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAaataat > 2:779735/1‑99 (MQ=255) tattatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatat < 2:1389965/99‑1 (MQ=255) tattatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatat > 1:457497/1‑99 (MQ=255) tattatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatat > 2:1252983/1‑99 (MQ=255) attatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatata > 2:247239/1‑99 (MQ=255) attatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatata < 2:439008/99‑1 (MQ=255) ttatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatataa > 2:1070999/1‑99 (MQ=255) ttatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatataa < 2:1310055/99‑1 (MQ=255) tatGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAataatataat > 1:1314216/1‑99 (MQ=255) tgtgAAGACTGTTTTGAAGCTACAAAACTAGGTACATCTGACTTGACATAATGTCCTCCCAACATTTTACTTGTATCATGGGCAAAGATAATATAATCa > 2:1509298/1‑99 (MQ=255) tgAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCata < 2:526753/99‑1 (MQ=255) tgAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCata < 1:899885/99‑1 (MQ=255) tgAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCata < 2:214053/99‑1 (MQ=255) aaGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATg > 1:851358/1‑99 (MQ=255) aaGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATg < 2:669213/99‑1 (MQ=255) gACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATggg < 2:1346346/99‑1 (MQ=255) cTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTa > 2:1420072/1‑99 (MQ=255) cTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTa > 1:898150/1‑99 (MQ=255) cTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTa > 1:611725/1‑99 (MQ=255) ttttGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAgtg > 1:613382/1‑99 (MQ=255) tGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGttt < 1:1149005/99‑1 (MQ=255) gAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTc < 1:886870/99‑1 (MQ=255) aaGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCa > 2:298542/1‑99 (MQ=255) gCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCata > 2:994651/1‑99 (MQ=255) gCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCata < 1:330655/99‑1 (MQ=255) cTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCatat > 2:389011/1‑99 (MQ=255) tACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATggg < 1:1090612/85‑1 (MQ=255) tACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATggg > 2:1090612/1‑85 (MQ=255) tACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATc < 1:1588594/99‑1 (MQ=255) cAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCAc > 1:678536/1‑99 (MQ=255) aaaCTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGt < 2:551327/99‑1 (MQ=255) aaaCTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGt < 1:1462642/99‑1 (MQ=255) aCTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTc < 2:1298545/99‑1 (MQ=255) cTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCg > 1:821650/1‑99 (MQ=255) gTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAat < 1:419183/58‑1 (MQ=255) gTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGatat < 2:1514754/99‑1 (MQ=255) gTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGatat > 1:1226063/1‑99 (MQ=255) aTCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGa < 2:508015/99‑1 (MQ=255) tCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGaa > 2:694138/1‑99 (MQ=255) tCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGaa < 2:813709/99‑1 (MQ=255) aCTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAg < 1:1582175/99‑1 (MQ=255) aCTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAg > 1:307844/1‑99 (MQ=255) cTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGt > 1:962053/1‑99 (MQ=255) cTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGt < 1:197176/99‑1 (MQ=255) cTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGt < 1:1635390/99‑1 (MQ=255) ttGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGtt < 2:169780/99‑1 (MQ=255) tGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTa < 2:515204/99‑1 (MQ=255) aCATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTtaa > 1:224119/1‑99 (MQ=255) aCATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTaaa > 1:1218011/1‑99 (MQ=255) cATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAAt > 2:842301/1‑99 (MQ=255) cATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAAt > 1:188964/1‑99 (MQ=255) cATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAAt > 2:937825/1‑99 (MQ=255) tAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCACATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTa > 1:559865/1‑99 (MQ=255) aaTGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTAt < 1:63217/99‑1 (MQ=255) aaTGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTAt < 2:1023909/99‑1 (MQ=255) aaTGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTAt < 2:1637285/99‑1 (MQ=255) aTGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATc < 1:878837/99‑1 (MQ=255) aTGTCCTCCCAAAATTTTACTTGCATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTAta > 2:1316668/1‑98 (MQ=255) tGTCCTCCCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCt > 1:958536/1‑99 (MQ=255) cctccCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGaa < 2:783602/99‑1 (MQ=255) cctccCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGaa < 1:1428106/99‑1 (MQ=255) ctccCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAAt < 2:1298435/99‑1 (MQ=255) tccCAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATc > 2:394970/1‑99 (MQ=255) ccAAAATTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTa < 1:1058634/99‑1 (MQ=255) aaaaTTTTACTTGTATCATTGTCAAAAATAATATAATGATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTaaa < 1:199050/99‑1 (MQ=255) aaaaTTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTaaa < 2:1353907/99‑1 (MQ=255) aaTTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAgc > 1:226354/1‑98 (MQ=255) aaTTTTACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGa < 1:1398585/99‑1 (MQ=255) ttttACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATa < 1:1070999/99‑1 (MQ=255) tttACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATaa < 2:492798/99‑1 (MQ=255) ttACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATaaa < 1:732068/99‑1 (MQ=255) ttACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATaaa > 2:1346529/1‑99 (MQ=255) ttACTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATaaa < 1:1152735/99‑1 (MQ=255) aCTTGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACg < 2:390437/99‑1 (MQ=255) ttGTATCATTGTCAAAAATAATATTATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGaa < 1:700044/99‑1 (MQ=255) tGTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAAt < 2:947977/99‑1 (MQ=255) gTATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTGCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATa > 1:704964/1‑99 (MQ=255) tATCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAg > 2:472645/1‑99 (MQ=255) aTCATTGTCAAAAATAATTTAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGt < 2:203273/99‑1 (MQ=255) aTCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGt < 2:1557505/99‑1 (MQ=255) aTCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGt > 2:770973/1‑99 (MQ=255) aTCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGt < 1:1167979/99‑1 (MQ=255) tCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGtt < 2:188964/99‑1 (MQ=255) tCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGtt < 1:1659756/99‑1 (MQ=255) tCATTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGtt < 2:851358/99‑1 (MQ=255) aTTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGTTTc < 1:834388/99‑1 (MQ=255) aTTGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGTTTc < 1:31407/99‑1 (MQ=255) ttGTCAAAAATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACAAATAGTTTCt < 2:1593528/99‑1 (MQ=255) | TGTAATATTATGTGAAGACTGTTTTGAAGCTACAAAACTAGGTACATCCGACTTGACATAATGTCCTCCCAAAATTTTACTTGTATCATTGTCAAACATAATATAATCATATGGGTAGTGTTTCATATCACGTTCGATATCAGAAATAGTTAAATTATCTGAATCTAAAGATAAACGAATAGTTTCT > CP002094/306271‑306457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |