BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002094	306,674	C→T	E199E (GAG→GAA)	LLNZ_01675 ←	Nisin biosynthesis two‑component system, response regulator NisR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002094	306,674	0	C	T	100.0%	455.2 / NA	111	E199E (GAG→GAA)	LLNZ_01675	Nisin biosynthesis two‑component system, response regulator NisR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (55/56); total (55/56)

Only 100 of 114 total aligned reads displayed.

TATTTTTTACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATACTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGATAG  >  CP002094/306578‑306769

tatTTTTTACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTc                                                                                               <  1:810252/99‑1 (MQ=255)

  ttttttACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCg                                                                                             >  2:257241/1‑99 (MQ=255)

   tttttACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCga                                                                                            >  2:86938/1‑99 (MQ=255)

      ttACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGAt                                                                                         >  2:448992/1‑99 (MQ=255)

       tACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGAtt                                                                                        >  1:1266987/1‑99 (MQ=255)

        aCCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTg                                                                                       <  1:1149803/99‑1 (MQ=255)

             tGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGa                                                                                  <  2:1135651/99‑1 (MQ=255)

             tGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGa                                                                                  >  1:848122/1‑99 (MQ=255)

               ccACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGaaa                                                                                <  1:1374746/99‑1 (MQ=255)

               ccACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGaaa                                                                                >  1:739061/1‑99 (MQ=255)

                cACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGaaaa                                                                               >  1:967053/1‑99 (MQ=255)

                cACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGaaaa                                                                               >  2:606936/1‑99 (MQ=255)

                  cTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAg                                                                             <  2:1187819/99‑1 (MQ=255)

                  cTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAg                                                                             >  1:1484671/1‑99 (MQ=255)

                   tGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGt                                                                            >  1:1202997/1‑99 (MQ=255)

                     aTACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGc                                                                          >  2:1225304/1‑99 (MQ=255)

                     aTACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGc                                                                          >  1:966885/1‑99 (MQ=255)

                     aTACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGc                                                                          >  2:826318/1‑99 (MQ=255)

                      tACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCa                                                                         >  2:1178461/1‑99 (MQ=255)

                      tACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCa                                                                         >  1:741382/1‑99 (MQ=255)

                      tACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCa                                                                         >  1:1548534/1‑99 (MQ=255)

                       aCCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCAt                                                                        <  2:973000/99‑1 (MQ=255)

                       aCCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCAt                                                                        <  1:289098/99‑1 (MQ=255)

                        cccAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCAtt                                                                       <  2:1413188/99‑1 (MQ=255)

                        cccAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCAtt                                                                       >  1:293920/1‑99 (MQ=255)

                          cAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAg                                                                     >  1:984276/1‑99 (MQ=255)

                            aGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGaa                                                                   <  1:1623256/99‑1 (MQ=255)

                              tCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAata                                                                 <  2:562215/99‑1 (MQ=255)

                                     aCCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATtcatca                                                          <  2:540671/99‑1 (MQ=255)

                                     aCCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATtcatca                                                          >  1:567609/1‑99 (MQ=255)

                                      ccGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTcatcat                                                         <  2:32082/99‑1 (MQ=255)

                                       cGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCata                                                        <  1:1306558/99‑1 (MQ=255)

                                       cGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCata                                                        <  2:881427/99‑1 (MQ=255)

                                       cGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCata                                                        <  2:1617964/99‑1 (MQ=255)

                                        gTTTGTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCatat                                                       <  1:1103510/99‑1 (MQ=255)

                                         tttttATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCAtata                                                      >  2:180276/1‑99 (MQ=255)

                                         tttttATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCAtata                                                      <  2:440814/99‑1 (MQ=255)

                                         tttttATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCAtata                                                      <  2:553246/99‑1 (MQ=255)

                                         tttttATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCAtata                                                      <  1:843587/99‑1 (MQ=255)

                                            ttATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGt                                                   <  1:732378/99‑1 (MQ=255)

                                            ttATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGt                                                   <  2:1315579/99‑1 (MQ=255)

                                             tATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGtc                                                  >  2:85310/1‑99 (MQ=255)

                                               tCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGtcat                                                >  1:1247177/1‑99 (MQ=255)

                                                 ggATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGtcatca                                              <  1:226501/99‑1 (MQ=255)

                                                  gATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTcatcat                                             >  2:773876/1‑99 (MQ=255)

                                                  gATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTcatcat                                             <  2:180106/99‑1 (MQ=255)

                                                   aTTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATa                                            >  1:512869/1‑99 (MQ=255)

                                                   aTTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATa                                            >  1:337414/1‑99 (MQ=255)

                                                    ttAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATaa                                           >  1:1602629/1‑99 (MQ=255)

                                                     tAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATaaa                                          <  1:1000637/99‑1 (MQ=255)

                                                      aaTATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAat                                         >  1:1489846/1‑99 (MQ=255)

                                                        tatCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAatat                                       <  2:676038/99‑1 (MQ=255)

                                                        tatCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAatat                                       >  2:709506/1‑99 (MQ=255)

                                                        tatCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAatat                                       >  2:758540/1‑99 (MQ=255)

                                                        tatCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAatat                                       <  1:1653384/99‑1 (MQ=255)

                                                          tCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATTATAAATATct                                     >  1:331025/1‑98 (MQ=255)

                                                          tCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATcc                                     >  1:145395/1‑99 (MQ=255)

                                                          tCGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATcc                                     <  2:448149/99‑1 (MQ=255)

                                                           cGTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCct                                    >  2:1241439/1‑99 (MQ=255)

                                                            gTATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCctc                                   >  1:1368901/1‑99 (MQ=255)

                                                             tATGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCctct                                  >  1:871421/1‑99 (MQ=255)

                                                              aTGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCctctc                                 <  2:1155669/99‑1 (MQ=255)

                                                              aTGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCctctc                                 >  2:1194176/1‑99 (MQ=255)

                                                               tGGTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTAATCATATACGTCATCATAAATATCctctct                                >  1:1080081/1‑99 (MQ=255)

                                                                ggTGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCtctctc                               >  2:850212/1‑99 (MQ=255)

                                                                  tGCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGt                             >  1:57916/1‑99 (MQ=255)

                                                                   gCAAACTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGta                            <  1:1226984/99‑1 (MQ=255)

                                                                     aaaCTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGtata                          <  2:296559/99‑1 (MQ=255)

                                                                      aaCTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATaa                         >  2:1533360/1‑99 (MQ=255)

                                                                       aCTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATaaa                        >  1:1204434/1‑99 (MQ=255)

                                                                       aCTTACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGCGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATaaa                        <  1:1582671/99‑1 (MQ=255)

                                                                         ttACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACt                      <  1:351890/99‑1 (MQ=255)

                                                                         ttACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACt                      <  2:110171/99‑1 (MQ=255)

                                                                          tACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACtt                     <  2:1602629/99‑1 (MQ=255)

                                                                          tACTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACtt                     <  2:182736/99‑1 (MQ=255)

                                                                           aCTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACttt                    <  2:962053/99‑1 (MQ=255)

                                                                           aCTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACttt                    >  2:439760/1‑99 (MQ=255)

                                                                            cTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACttca                   >  1:1118468/1‑97 (MQ=255)

                                                                            cTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTa                   >  1:1082008/1‑99 (MQ=255)

                                                                            cTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTa                   >  1:255375/1‑99 (MQ=255)

                                                                            cTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTa                   <  1:993805/99‑1 (MQ=255)

                                                                            cTCCTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTa                   >  1:1570120/1‑99 (MQ=255)

                                                                               cTAATCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGaa                >  2:1070082/1‑99 (MQ=255)

                                                                                 aaTCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGt              <  2:1062503/99‑1 (MQ=255)

                                                                                  aTCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGtt             <  2:1193530/99‑1 (MQ=255)

                                                                                  aTCTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGtt             >  2:673776/1‑99 (MQ=255)

                                                                                    cTGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCg           <  2:1004639/99‑1 (MQ=255)

                                                                                     tGATAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGt          <  1:317853/99‑1 (MQ=255)

                                                                                     agATAAATATATTCCGAGATTGACCGAAAAAATGCATAAGAATATTCATCATATACGTCTTCATAAATATCCCCTCTCCTATGAACTATAGATGTTCGt          <  2:184885/98‑1 (MQ=255)

                                                                                       aTAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTtg        <  1:114120/99‑1 (MQ=255)

                                                                                       aTAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTtg        >  1:1444111/1‑99 (MQ=255)

                                                                                        tAAATATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTtgt       >  1:1122777/1‑99 (MQ=255)

                                                                                          aaTATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGa     <  1:586270/99‑1 (MQ=255)

                                                                                          aaTATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGa     >  1:1347729/1‑99 (MQ=255)

                                                                                          aaTATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGa     >  2:670470/1‑99 (MQ=255)

                                                                                          aaTATATTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGa     <  1:1298433/99‑1 (MQ=255)

                                                                                           atatatTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGAt    >  1:522733/1‑99 (MQ=255)

                                                                                           atatatTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGAt    >  1:919453/1‑99 (MQ=255)

                                                                                            tataTTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGATa   <  2:1202997/99‑1 (MQ=255)

                                                                                             atatTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGATAg  <  1:448992/99‑1 (MQ=255)

TATTTTTTACCCATGCCACTGATACCCAAGTCCCCGAACCGTTTTTATCGGATTAATATCGTATGGTGCAAACTTACTCCTAATCTGATAAATATACTCCGAGATTGACCGAAAAAGTGCATTAGAATATTCATCATATACGTCATCATAAATATCCTCTCTCGTATAAACTTTAGAAGTTCGTTGTGATAG  >  CP002094/306578‑306769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`