Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	2,331,461	(T)8→7	intergenic (‑905/+261)	ECW_RS11680 ← / ← ECW_RS11685	AAA family ATPase/replication endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	2,331,454	0	T	.	100.0%	182.0 / NA	42	intergenic (‑898/+268)	ECW_RS11680/ECW_RS11685	AAA family ATPase/replication endonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/26);  total (16/26)

CATTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGTTTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGAC  >  NC_017635/2331406‑2331497                                                 |                                            cATTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtt                                            >  1:1493376/1‑50 (MQ=255)  aTTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt                                           >  1:485816/1‑50 (MQ=255)  aTTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt                                           >  1:364425/1‑50 (MQ=255)  aTTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt                                           >  2:1302278/1‑50 (MQ=255)  aTTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGACAGGGTGGttt                                           <  1:1235061/50‑1 (MQ=255)   ttATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttt                                          <  2:1938401/50‑1 (MQ=255)   ttATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttt                                          <  2:197430/50‑1 (MQ=255)    tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttttt                                         >  1:1456554/1‑50 (MQ=255)     aTGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttt                                        >  2:1614998/1‑50 (MQ=255)      tGCTTTAATATTCTTCAAACAAAAAAACCACCTGCCAGGGTGGttttttt                                       <  2:485668/50‑1 (MQ=255)      tGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttttttt                                       <  1:560346/50‑1 (MQ=255)      tGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttttttt                                       <  1:1897434/50‑1 (MQ=255)      tGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttt                                        <  1:140718/49‑1 (MQ=255)       gCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttttg                                      >  1:2054785/1‑49 (MQ=255)       gCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttttg                                      >  2:592947/1‑49 (MQ=255)        cTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttttgc                                     >  2:126814/1‑48 (MQ=255)        cTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttttgc                                     >  1:158885/1‑48 (MQ=255)        cTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttttgc                                     >  1:1277572/1‑48 (MQ=255)            aaTATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc                                 <  1:1811290/49‑1 (MQ=38)            aaTATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc                                 <  2:906521/49‑1 (MQ=38)             atatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca                               >  2:940876/1‑50 (MQ=39)              tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcat                              >  2:1054335/1‑50 (MQ=39)                 gCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatcat                           >  1:1637546/1‑50 (MQ=39)                  cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatcat                           <  2:1109628/49‑1 (MQ=38)                  cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCatcatc                          <  1:491897/50‑1 (MQ=39)                  cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCatcatc                          <  1:1037585/50‑1 (MQ=39)                     caaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAtcatca                         <  2:131714/48‑1 (MQ=38)                     caaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAtcatca                         <  2:1061545/48‑1 (MQ=38)                     caaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCaa                        >  1:682425/1‑49 (MQ=38)                     caaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCACCaa                        >  1:1561148/1‑49 (MQ=38)                      aaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAAt                       <  1:775296/49‑1 (MQ=38)                      aaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAAt                       <  2:2016740/49‑1 (MQ=38)                      aaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAAt                       <  2:1907338/49‑1 (MQ=38)                      aaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATa                      >  2:1647838/1‑50 (MQ=39)                      aaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATa                      >  2:1791541/1‑50 (MQ=39)                       aacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATAc                     <  2:610271/50‑1 (MQ=39)                        acaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATAc                     >  1:28938/1‑49 (MQ=38)                        acaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACg                    <  1:75009/50‑1 (MQ=39)                          aaaaaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGAt                  >  1:689885/1‑50 (MQ=39)                          aaaaaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGAt                  <  2:1706968/50‑1 (MQ=39)                          aaaaaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGAt                  <  2:1824585/50‑1 (MQ=39)                           aaaaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATg                 <  2:626042/50‑1 (MQ=39)                            aaaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATg                 >  2:2081770/1‑49 (MQ=39)                            aaaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGa                <  2:343077/50‑1 (MQ=255)                             aaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGaa               <  2:1241771/50‑1 (MQ=255)                             aaaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGa                >  2:938076/1‑49 (MQ=39)                              aaaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGa                >  1:1922961/1‑48 (MQ=39)                               aaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGa                >  1:1186375/1‑47 (MQ=39)                               aaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAg             <  1:2055819/50‑1 (MQ=255)                               aaCCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAg             <  1:1140090/50‑1 (MQ=255)                                accaccTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGa            <  2:1263916/50‑1 (MQ=255)                                 ccaccTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGAc           <  2:1415801/50‑1 (MQ=255)                                      tGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGAc       >  1:885817/1‑49 (MQ=39)                                       gCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGAc       >  2:575949/1‑48 (MQ=39)                                        ccAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGAc       >  2:1557647/1‑47 (MQ=39)                                        ccAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACaa     <  2:1652045/49‑1 (MQ=39)                                        ccAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACa      <  2:1686783/48‑1 (MQ=39)                                        ccAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACa      <  2:1654669/48‑1 (MQ=39)                                        ccAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACa      <  2:297310/48‑1 (MQ=39)                                          aGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGAc  <  1:338894/50‑1 (MQ=255)                                                 |                                            CATTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGTTTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGAC  >  NC_017635/2331406‑2331497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑