Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	1,679,419	+C	intergenic (‑9/+146)	gadC ← / ← gadB	acid resistance gamma‑aminobutyrate antiporter GadC/glutamate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	1,679,415	1	.	C	100.0%	94.1 / NA	24	intergenic (‑5/+150)	gadC/gadB	acid resistance gamma‑aminobutyrate antiporter GadC/glutamate decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (15/9);  total (15/9)

AATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑CCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCT  >  NC_017635/1679367‑1679459                                                  |                                             aaTGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑c                                             >  1:1528964/1‑50 (MQ=255)  aTGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑cc                                            <  1:1369876/50‑1 (MQ=255)  aTGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑c                                             <  2:1634075/49‑1 (MQ=255)   tgtgAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑ccc                                           <  2:1862945/50‑1 (MQ=255)   tgtgAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑ccc                                           <  1:995801/50‑1 (MQ=255)   tgtgAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑ccc                                           <  1:2031223/50‑1 (MQ=255)     tgAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑ccccc                                         <  2:726863/50‑2 (MQ=255)               aGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTa                                >  2:552887/1‑50 (MQ=255)                  tttACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTc                             >  1:585590/1‑50 (MQ=255)                  tttACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTc                             >  2:189251/1‑50 (MQ=255)                    tACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTc                             <  1:1508808/48‑1 (MQ=255)                    tACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCt                           >  2:1079745/1‑50 (MQ=255)                     aCCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCt                           <  1:450945/49‑1 (MQ=255)                      ccTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTg                          <  2:2081961/49‑1 (MQ=255)                      ccTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGt                         <  1:629742/50‑1 (MQ=255)                      ccTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGt                         >  1:802260/1‑50 (MQ=255)                      ccTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGt                         >  2:1798733/1‑50 (MQ=255)                       ctgtctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTc                        >  1:1738273/1‑50 (MQ=255)                        tgtctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCg                       >  2:499300/1‑50 (MQ=255)                         gtctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCg                       >  1:1964206/1‑49 (MQ=255)                         gtctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCg                       >  1:1739915/1‑49 (MQ=255)                             gtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAcc                  >  1:1857698/1‑50 (MQ=255)                              tACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAc                   <  1:1049564/48‑1 (MQ=255)                                 tGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAc              >  1:984752/1‑50 (MQ=255)                                  gATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc             >  2:1109881/1‑50 (MQ=255)                                   aTGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc             <  2:409781/49‑1 (MQ=255)                                   aTGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCt            >  1:1164954/1‑50 (MQ=255)                                      tAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGt          <  2:1070913/49‑1 (MQ=255)                                        gCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGtttt       >  1:657556/1‑50 (MQ=255)                                          cATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGt     <  1:861069/50‑1 (MQ=255)                                           atatTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGtt    <  2:1783424/50‑1 (MQ=255)                                            tattatcCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTc   >  1:758209/1‑50 (MQ=255)                                             attatcCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt  >  2:1363872/1‑50 (MQ=255)                                                  |                                             AATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTAT‑CCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCT  >  NC_017635/1679367‑1679459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑