Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	2,292,091	+G	coding (36/39 nt)	ECW_RS27980 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	2,292,091	1	.	G	100.0%	54.3 / NA	28	V12V (GTA→GTC)	ECW_RS27980	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (9/19);  total (9/19)

AGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTAT‑ACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTT  >  NC_017635/2292042‑2292134                                                   |                                            agagCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTtat                                              >  1:130474/1‑50 (MQ=255) agagCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTtat                                              >  2:512085/1‑50 (MQ=255)  gagCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTtat                                              <  1:1232153/49‑1 (MQ=255)       gggTTCCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTaa                                        <  2:1709490/50‑1 (MQ=17)         gTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAaga                                      <  2:809898/50‑1 (MQ=17)             cccccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGca                                   <  1:1988563/49‑1 (MQ=17)               cccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGcacca                                <  1:1893003/50‑1 (MQ=17)               cccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGcacca                                >  1:2013267/1‑50 (MQ=17)                 cGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACg                              <  2:1186205/50‑1 (MQ=17)                  gCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACga                             <  1:841354/50‑1 (MQ=17)                    ccTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACgatg                           <  1:724434/50‑1 (MQ=17)                      tGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACgatgat                         >  2:1429234/1‑50 (MQ=17)                         tAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAg                      >  1:194484/1‑50 (MQ=17)                           gTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTa                    <  1:709783/50‑1 (MQ=17)                           gTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTa                    <  2:851590/50‑1 (MQ=17)                           gTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTa                    <  2:2054033/50‑1 (MQ=17)                                aGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCt                 <  1:183666/48‑1 (MQ=17)                                 gTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtca              <  1:794160/50‑1 (MQ=17)                                  tAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcat             <  1:1907547/50‑1 (MQ=17)                                   aaGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcat             >  2:1114226/1‑49 (MQ=17)                                    aGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca           <  1:396579/50‑1 (MQ=17)                                    aGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca           <  1:294359/50‑1 (MQ=17)                                      cGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTcatcat          >  1:1897140/1‑49 (MQ=14)                                      cGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATg         <  1:1812204/50‑1 (MQ=14)                                       ggggAAGCTTATGACTAAGAGCACCACGATGATGATTAGCTTCATCATGa        <  2:990173/50‑1 (MQ=14)                                       ggggAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGa        <  1:2033675/50‑1 (MQ=14)                                       ggggAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGa        >  2:1592104/1‑50 (MQ=14)                                          gAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGAccc     >  2:2031298/1‑50 (MQ=11)                                           aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCt    >  1:610709/1‑50 (MQ=11)                                           aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCt    >  2:1143472/1‑50 (MQ=11)                                             gCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCttt  <  1:1737219/50‑1 (MQ=1)                                                   |                                            AGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTAT‑ACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTT  >  NC_017635/2292042‑2292134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑