Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	3,242,636	+A	intergenic (‑1/+279)	ygfF ← / ← gcvP	SDR family oxidoreductase/aminomethyl‑transferring glycine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	3,242,635	1	.	A	100.0%	89.0 / NA	25	M1M (GTG→TTG) †	ygfF	SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (12/13);  total (12/13)

GCCAACAGTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCAC‑AGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGA  >  NC_017635/3242586‑3242679                                                   |                                             gcCAACAGTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTcac                                               <  2:244168/50‑1 (MQ=255)  ccAACAGTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTcac‑a                                             <  2:1259571/50‑1 (MQ=255)    aaCAGTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTcac‑aag                                           <  1:1845465/50‑3 (MQ=255)        gTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCt                                        <  1:2053932/50‑1 (MQ=255)        gTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCt                                        <  2:1687352/50‑1 (MQ=255)         tAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCta                                       <  1:533849/50‑1 (MQ=255)         tAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCta                                       <  2:1460778/50‑1 (MQ=255)           aTGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCtata                                     <  1:1172960/50‑1 (MQ=255)           aTGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCtata                                     <  1:1711023/50‑1 (MQ=255)            tGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCTATAg                                    <  2:582518/50‑1 (MQ=255)                 ttGCCCGCCCGATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCAtt                               <  2:315352/50‑1 (MQ=255)                    cccgcccgATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCACTTg                             <  2:272703/49‑1 (MQ=38)                       gcccgATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGtttt                         >  1:241048/1‑50 (MQ=255)                       gcccgATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGtttt                         >  2:1169087/1‑50 (MQ=255)                       gcccgATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGtttt                         >  1:1003870/1‑50 (MQ=255)                          cgATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGTTTTTcc                      <  2:1326262/50‑1 (MQ=255)                          cgATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGTTTTTcc                      >  2:1209264/1‑50 (MQ=255)                           gATGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGTTTTTCCg                     <  2:841953/50‑1 (MQ=255)                             tGCCGCGACTGCCACCGGTCACAAGTGCTATAGCCATTTGTTTTTcc                      >  2:933141/1‑47 (MQ=255)                                 gcgACTGCCACCGGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGaaa                >  1:1587897/1‑49 (MQ=255)                                       gCCACCGGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAaattaat         >  2:991005/1‑50 (MQ=255)                                        ccACCGGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAaattaat         <  1:1750334/49‑1 (MQ=255)                                         cACCGGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTc       <  2:651883/50‑1 (MQ=255)                                           ccGGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGt     >  1:525899/1‑50 (MQ=255)                                             ggTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGtt    >  2:1552325/1‑49 (MQ=255)                                             ggTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGtt    >  1:476944/1‑49 (MQ=255)                                             ggTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGtt    >  2:467451/1‑49 (MQ=255)                                              gTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGa  >  2:1542880/1‑50 (MQ=255)                                                   |                                             GCCAACAGTAATGCAGTTGCCCGCCCGATGCCGCGACTGCCACCGGTCAC‑AGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGA  >  NC_017635/3242586‑3242679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑