Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	4,667,282	(T)8→9	intergenic (+46/‑80)	rlmB → / → yjfI	23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB/DUF2170 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	4,667,274	1	.	T	100.0%	190.5 / NA	54	intergenic (+38/‑88)	rlmB/yjfI	23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (37/17);  total (37/17)

CAGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCAAATA  >  NC_017635/4667225‑4667318                                                   |                                             cAGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATgg                                               >  2:1012479/1‑50 (MQ=255) cAGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATgg                                               >  2:214624/1‑50 (MQ=255) cAGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATgg                                               >  2:1901748/1‑50 (MQ=255)  aGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATgg                                               <  2:1644450/49‑1 (MQ=255)  aGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑t                                             <  2:334760/50‑1 (MQ=255)  aGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑t                                             >  2:1517237/1‑50 (MQ=255)     gcAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑tttt                                          <  1:666291/50‑1 (MQ=255)      cAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttt                                         >  2:1069715/1‑50 (MQ=255)       aGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑tttttt                                        >  2:1962392/1‑50 (MQ=255)          tAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttttttt                                     >  1:115567/1‑49 (MQ=255)          tAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttttttt                                     >  2:1074686/1‑49 (MQ=255)           aGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑tttttttttg                                    >  2:1566449/1‑48 (MQ=255)                gTCTGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCta                                 <  1:1483229/49‑1 (MQ=255)                gTCTGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCta                                 >  1:1478808/1‑49 (MQ=255)                 tCTGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCtata                               <  2:1510768/50‑1 (MQ=255)                  cTGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAg                              >  2:1699376/1‑50 (MQ=255)                  cTGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAg                              >  2:1576433/1‑50 (MQ=255)                   tGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGc                             >  1:1560002/1‑50 (MQ=255)                   tGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGc                             >  1:397659/1‑50 (MQ=255)                   tGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGc                             >  1:373111/1‑50 (MQ=255)                    gAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCt                            >  2:646865/1‑50 (MQ=255)                     aaTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAg                              <  2:249978/47‑1 (MQ=255)                     aaTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTg                           <  2:1595598/50‑1 (MQ=255)                     aaTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTg                           >  2:1079610/1‑50 (MQ=255)                      aTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTgg                          >  2:213931/1‑50 (MQ=255)                       ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTgg                          >  1:1661887/1‑49 (MQ=255)                       ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTgg                          >  1:1156187/1‑49 (MQ=255)                        tATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGt                         >  1:557541/1‑49 (MQ=255)                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGt                         >  1:748127/1‑47 (MQ=255)                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGt                         >  2:21398/1‑47 (MQ=255)                           gAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGt                         >  2:1169363/1‑46 (MQ=255)                           gAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGt                         >  1:2003878/1‑46 (MQ=255)                             aaaGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATa                   <  2:1367052/50‑1 (MQ=255)                              aaGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATaa                  >  1:138292/1‑50 (MQ=255)                               aGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAAt                 <  2:684848/50‑1 (MQ=255)                               aGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAAt                 <  2:1604135/50‑1 (MQ=255)                                gCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAAt                 >  1:1807921/1‑49 (MQ=255)                                gCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAAt                 >  2:370067/1‑49 (MQ=255)                                gCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAAt                 >  2:1583230/1‑49 (MQ=255)                                 ccATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTa               <  1:304719/50‑1 (MQ=255)                                  cATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTAc              >  2:1016370/1‑50 (MQ=255)                                  cATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTAc              <  2:1329434/50‑1 (MQ=255)                                  cATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTAc              >  2:388638/1‑50 (MQ=255)                                  cATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTAc              >  1:244002/1‑50 (MQ=255)                                   aTCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg             >  2:436757/1‑50 (MQ=255)                                   aTCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg             >  1:1767763/1‑50 (MQ=255)                                   aTCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg             >  1:1159577/1‑50 (MQ=255)                                    tCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg             >  2:918645/1‑49 (MQ=255)                                    tCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg             >  2:741643/1‑49 (MQ=255)                                    tCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg             >  1:1470579/1‑49 (MQ=255)                                    tCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt            <  1:952995/50‑1 (MQ=255)                                     ccAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa           >  2:1708113/1‑50 (MQ=255)                                      cAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAc          >  1:1593487/1‑50 (MQ=255)                                         aTTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTg        >  1:1567580/1‑49 (MQ=255)                                         aTTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTg        >  2:1440519/1‑49 (MQ=255)                                          tttGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGc       >  2:605444/1‑49 (MQ=255)                                          tttGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGc       >  1:1096156/1‑49 (MQ=255)                                          tttGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCa      <  2:889922/50‑1 (MQ=255)                                          tttGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCa      <  2:295979/50‑1 (MQ=255)                                            tGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCaa     <  1:1924088/49‑1 (MQ=255)                                             ggATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCaaa    >  1:124565/1‑49 (MQ=255)                                             ggATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCaaa    <  1:1799367/49‑1 (MQ=255)                                              gATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCAAata  >  1:3543/1‑50 (MQ=255)                                              gATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCAAata  <  1:1933712/50‑1 (MQ=255)                                              gATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCAAata  <  1:131960/50‑1 (MQ=255)                                              gATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCAAata  <  2:2014034/50‑1 (MQ=255)                                                   |                                             CAGCGCAGCTAGTTCGTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTACTGCAAATA  >  NC_017635/4667225‑4667318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑