Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	2,312,974	G→A	L135L (CTC→CTT)	ECW_RS11560 ←	phage tail sheath protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	2,312,974	0	G	A	100.0%	19.3 / NA	15	L135L (CTC→CTT)	ECW_RS11560	phage tail sheath protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

CCAGTGCAACAGCCACCTCTTTGGTATCCAGTCCCGGCACGCCGAGAATACGCGGTTTAACACCGGTTACCGACT  >  NC_017635/2312931‑2313005                                            |                                ccAGTGCAACAGCCACCTCTTTGGTATCCAGCCCCGGCACACCAAGAAt                            <  1:1755133/49‑1 (MQ=11)         aCAGCCACCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGtt                    >  1:424003/1‑49 (MQ=11)           aGCCACCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTaa                 >  1:1610065/1‑50 (MQ=11)           aGCCACCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTaa                 >  2:313821/1‑50 (MQ=11)              cACCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAacac              >  2:1655002/1‑50 (MQ=11)               aCCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACAcc             <  1:564634/50‑1 (MQ=11)               aCCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACAcc             <  1:747575/50‑1 (MQ=11)               aCCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACAcc             <  2:1937795/50‑1 (MQ=11)               aCCTCTTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACAcc             <  2:980621/50‑1 (MQ=11)                   ctTTGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACACCGGt          >  1:2053110/1‑49 (MQ=11)                      tGGTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACACCGGTTAcc      >  1:1149883/1‑50 (MQ=11)                       ggTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACACCGGTTAcc      <  2:1153857/49‑1 (MQ=11)                        gTATCCAGCCCCGGCACACCAAGAATACGCGGTTTAACACCGGTTACCg     <  1:679295/49‑1 (MQ=11)                         tATCCAGCCCCTGCACACCAAGAATACGCGGTTTAACACCGGTTACCGAc   >  2:1206768/1‑50 (MQ=11)                          aTCCAGCCCCGGCACACCAAGAATACGCGGTTTAACACCGGTTACCGAct  >  1:323762/1‑50 (MQ=11)                                            |                                CCAGTGCAACAGCCACCTCTTTGGTATCCAGTCCCGGCACGCCGAGAATACGCGGTTTAACACCGGTTACCGACT  >  NC_017635/2312931‑2313005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑