| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_017635 | 2,860,853 | C→A | R442S (AGG→AGT) | qseE ← | two component system sensor histidine kinase QseE/GlrK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_017635 | 2,860,853 | 0 | C | A | 96.8% | 113.5 / NA | 31 | R442S (AGG→AGT) | qseE | two component system sensor histidine kinase QseE/GlrK |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (18/12); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCC > NC_017635/2860806‑2860894 | ctctCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACt < 2:352231/50‑1 (MQ=255)ctctCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACt < 1:1240905/50‑1 (MQ=255) atcgtcgACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTg < 1:1207884/48‑1 (MQ=255) cgtcgACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGc > 2:1051176/1‑49 (MQ=255) gtcgACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGc < 1:795586/48‑1 (MQ=255) gACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATg < 2:182942/49‑1 (MQ=255) gACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATg < 1:1506563/49‑1 (MQ=255) aCCAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCt > 2:1404348/1‑50 (MQ=255) ccAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCt > 1:2035649/1‑49 (MQ=255) cAGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCTTa < 1:875416/50‑1 (MQ=255) aGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCTTaa > 1:1172961/1‑50 (MQ=255) aGATACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCTTaa > 2:2039489/1‑50 (MQ=255) tACAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCTTAATcc < 1:1149090/50‑1 (MQ=255) cAGTTCCCCTTGCATACGGCGAATACAATCACTGGCAATGCTTAATccc < 2:1961698/49‑1 (MQ=255) tCCCCTTGCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGAcc > 1:817530/1‑50 (MQ=255) ccccTTGCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCg > 1:642684/1‑50 (MQ=255) ccTTGCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCt < 1:1485786/50‑1 (MQ=255) ccTTGCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCt > 1:106749/1‑50 (MQ=255) cTTGCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTg > 1:1816308/1‑50 (MQ=255) ttGCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGc > 1:884006/1‑50 (MQ=255) gCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGccc > 2:1442823/1‑50 (MQ=255) gCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGccc > 1:1208717/1‑50 (MQ=255) gCATACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGccc > 1:121866/1‑50 (MQ=255) aTACGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCtt < 1:1178461/50‑1 (MQ=255) aCGGCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCTTCa > 2:1056971/1‑50 (MQ=255) ggCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCTTCAcc < 2:1964380/50‑1 (MQ=255) ggCGAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCTTCAcc > 1:1235430/1‑50 (MQ=255) gAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcc > 1:546541/1‑50 (MQ=255) gAATACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcc > 2:222211/1‑50 (MQ=255) aaTACAATCACTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGccc > 2:48908/1‑50 (MQ=255) aTACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcccc < 2:790650/50‑1 (MQ=255) | CTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCC > NC_017635/2860806‑2860894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |