Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	3,841,812	G→A	G12S (GGC→AGC)	gorA →	glutathione‑disulfide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	3,841,812	0	G	A	100.0%	63.9 / NA	18	G12S (GGC→AGC)	gorA	glutathione‑disulfide reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

AAGGACACTTTGTCATGACTAAACACTATGATTACATCGCCATCGGCGGCGGCAGCGGCGGTATCGCCTCCATCAACCGCGCGGCTATGTACGG  >  NC_017635/3841765‑3841858                                                |                                               aaGGACACTTTGTCATGACTAAACACTATGATTACATCGCCATcggcagc                                              >  1:1947969/1‑50 (MQ=255)    gACACTTTGTCATGACTAAACACTATGATTACATCGCCATCggcagcggc                                           <  2:1307341/50‑1 (MQ=255)       acTTTGTCATGACTAAACACTATGATTACATCGCCATCggcagcggcagc                                        >  1:961586/1‑50 (MQ=255)           tGTCATGACTAAACACTATGATTACATCGCCATCGGCAGCGGCAgcggcg                                    >  2:603477/1‑50 (MQ=255)           tGTCATGACTAAACACTATGATTACATCGCCATCGGCAGCGGCAgcggcg                                    >  1:1597136/1‑50 (MQ=255)               aTGACTAAACACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTa                                 <  1:205725/49‑1 (MQ=255)               aTGACTAAACACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTAt                                >  1:2022702/1‑50 (MQ=255)                    tAAACACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGGATCGccc                           >  2:1703881/1‑49 (MQ=38)                      aaCACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTc                          <  1:178653/49‑1 (MQ=255)                      aaCACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTc                          >  1:24543/1‑49 (MQ=255)                      aaCACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTc                          <  1:468296/49‑1 (MQ=255)                      aaCACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTc                          >  1:720665/1‑49 (MQ=255)                      aaCACTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGAATCGCcccc                         >  2:1028514/1‑50 (MQ=38)                         acTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATc                      <  2:1912405/50‑1 (MQ=255)                         acTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATc                      <  1:1463036/50‑1 (MQ=255)                          cTATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATCa                     <  1:449483/50‑1 (MQ=255)                           tATGATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATCaa                    >  2:713784/1‑50 (MQ=255)                              gATTACATCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATCAACcg                 >  1:1702560/1‑50 (MQ=255)                                    aTCGCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATCAACCGCGCGGc           <  1:41863/50‑1 (MQ=255)                                       gCCATCGGCAGCGGCAGCGGCGGTATCGCCTCCATCAACCGCGCGGCTa         <  2:1298324/49‑1 (MQ=255)                                         cATCGGCAGCGGCAGCGGCAGTATCGCCTCCATCAACCGCGCGGCTATGt      <  2:1716218/50‑1 (MQ=39)                                             ggcagcggcagcGGCGGTATCGCCTCCATCAACCGCGCGGCTATGTACgg  >  1:89269/1‑50 (MQ=255)                                             ggcagcggcagcGGCGGTATCGCCTCCATCAACCGCGCGGCTATGTACg   <  2:143449/49‑1 (MQ=255)                                             ggcagcggcagcGGCGGTATCGCCTCCATCAACCGCGCGGCTATGTACg   <  2:1038809/49‑1 (MQ=255)                                             ggcagcggcagcGGCGGTATCGCCTCCATCAACCGCGCGGCTATGTACg   <  2:298041/49‑1 (MQ=255)                                                |                                               AAGGACACTTTGTCATGACTAAACACTATGATTACATCGCCATCGGCGGCGGCAGCGGCGGTATCGCCTCCATCAACCGCGCGGCTATGTACGG  >  NC_017635/3841765‑3841858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑