Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	4,336,992	C→T	I492I (ATC→ATT)	ECW_RS21660 →	replication endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	4,336,992	0	C	T	100.0%	37.4 / NA	12	I492I (ATC→ATT)	ECW_RS21660	replication endonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

ACACTGCAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATCCCGCAATTTAAAACGGTTGGCCTGCCGACAAT  >  NC_017635/4336949‑4337024                                            |                                 acacTGCAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATTCCGCaa                            <  2:148561/50‑1 (MQ=255)  cacTGCAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATTCCGCAAt                           >  1:1092672/1‑50 (MQ=255)   acTGCAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATTCCGCAAtt                          >  2:1158557/1‑50 (MQ=255)    cTGCAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATTCCGCAAttt                         <  2:505534/50‑1 (MQ=255)       cAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATTCCGCAATTTaa                       >  1:826138/1‑49 (MQ=255)                 tACCGCATGGGCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGcc            >  2:1955758/1‑50 (MQ=255)                  aCCGCATGGGCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGcc            <  1:328664/49‑1 (MQ=255)                     gCATGGGCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGCCTGcc        >  2:1574604/1‑50 (MQ=255)                     gCATGGGCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGCCTGcc        >  2:906131/1‑50 (MQ=255)                        tGGGCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGCCTGCCGa      >  1:1113396/1‑49 (MQ=255)                          ggCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGCCTGCCGACaa   <  1:1332534/50‑1 (MQ=255)                           gCGTCTACGTGGCGCATTCCGCAATTTAAAACGGTTGGCCTGCCGACAAt  <  2:999847/50‑1 (MQ=255)                                            |                                 ACACTGCAGCGGCTGTTACCGCATGGGCGTCTACGTGGCGCATCCCGCAATTTAAAACGGTTGGCCTGCCGACAAT  >  NC_017635/4336949‑4337024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑