Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	4,344,085	G→A	Y293Y (TAC→TAT)	ECW_RS21690 ←	terminase ATPase subunit family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	4,344,085	0	G	A	100.0%	30.9 / NA	10	Y293Y (TAC→TAT)	ECW_RS21690	terminase ATPase subunit family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

AACAGTTCACCGGACCAGAACGGGTAGGCGTCGTGCGCCAGCGTGGACGGGGTGGAGAAATAGGTC  >  NC_017635/4344062‑4344127                        |                                           aaCAGTTCACCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACgg                  >  1:1086013/1‑50 (MQ=255) aaCAGTTCACCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACg                   <  1:295913/49‑1 (MQ=255)    aGTTCACCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGt               <  1:1380772/50‑1 (MQ=255)      ttCACCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTgg             >  2:1868154/1‑50 (MQ=255)      ttCACCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTg              >  1:1563169/1‑49 (MQ=255)       tCACCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTg              >  1:715685/1‑48 (MQ=255)         aCCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTGgag           <  1:1573556/49‑1 (MQ=255)         aCCGGACCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTGgag           <  1:36938/49‑1 (MQ=255)              aCCAGAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTGGAGAAATAg     >  1:1024830/1‑50 (MQ=255)                  gAACGGATAGGCGTCGTGCGCCAGCGTGGACGGGGTGGAGAAATAGGTc  >  1:340535/1‑49 (MQ=255)                        |                                           AACAGTTCACCGGACCAGAACGGGTAGGCGTCGTGCGCCAGCGTGGACGGGGTGGAGAAATAGGTC  >  NC_017635/4344062‑4344127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑