Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	4,729,669	C→G	V46L (GTG→CTG)	pyrI ←	aspartate carbamoyltransferase regulatory subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	4,729,669	0	C	G	100.0%	186.6 / NA	45	V46L (GTG→CTG)	pyrI	aspartate carbamoyltransferase regulatory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (32/13);  total (32/13)

GATTTTGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCACACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGAACAGA  >  NC_017635/4729622‑4729712                                                |                                            gATTTTGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCagac                                           >  2:254092/1‑50 (MQ=255)   ttttGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGAcc                                          >  2:1677095/1‑49 (MQ=255)   ttttGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCg                                         <  2:1055551/50‑1 (MQ=255)   ttttGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCg                                         <  2:1021378/50‑1 (MQ=255)   ttttGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGAAAGTTCAGACCg                                         <  1:962567/50‑1 (MQ=255)    tttGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGa                                        >  1:634928/1‑50 (MQ=255)     ttGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGAt                                       >  2:743889/1‑50 (MQ=255)      tGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATg                                      >  1:131919/1‑50 (MQ=255)       gATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATgg                                     >  2:36143/1‑50 (MQ=255)       gATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATgg                                     >  2:1425951/1‑50 (MQ=255)        aTCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATgg                                     <  2:1450078/49‑1 (MQ=255)        aTCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGt                                    >  2:701180/1‑50 (MQ=255)         tCAGATCTTTGCGGCCCATCTCGCCATAAGGCAAGTTCAGACCGATGGt                                    >  1:617892/1‑49 (MQ=255)         tCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTg                                   <  1:935522/50‑1 (MQ=255)          cAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGa                                  >  2:124734/1‑50 (MQ=255)           aGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGAt                                 >  1:410670/1‑50 (MQ=255)           aGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGAt                                 >  2:715097/1‑50 (MQ=255)             aTCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATgc                               >  2:1402172/1‑50 (MQ=255)                 ttGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCt                            >  2:1257035/1‑49 (MQ=255)                  tGCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATgc                               <  1:223248/45‑1 (MQ=255)                   gCGGCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGAt                         >  1:1517273/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  2:865501/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  2:307364/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  2:1158952/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  2:861209/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  1:1524297/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  2:996834/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  1:2042711/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  2:1852434/1‑50 (MQ=255)                     ggCCCATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATcc                       >  1:424997/1‑50 (MQ=255)                       cccATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGt                     >  1:626467/1‑50 (MQ=255)                         cATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGttt                   >  2:505549/1‑50 (MQ=255)                         cATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGttt                   >  1:482825/1‑50 (MQ=255)                         cATCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGttt                   >  1:1188790/1‑50 (MQ=255)                          aTCTCGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTc                  <  1:1892603/50‑1 (MQ=255)                          aTCTCGCCAGAAGGCAAGTTCAGACCGATGGAGATGCGCTGATCCGTTTc                  >  1:1892119/1‑50 (MQ=255)                           tctcGCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTct                 >  1:462098/1‑49 (MQ=255)                               gCCAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTc              <  1:1300682/49‑1 (MQ=255)                                ccAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAg            >  1:1278377/1‑50 (MQ=255)                                 cAGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGc           >  2:1025174/1‑50 (MQ=255)                                  aGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCt          <  2:1895926/50‑1 (MQ=255)                                  aGAAGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCt          <  2:1157703/50‑1 (MQ=255)                                    aaGGCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCTTg        <  2:533401/50‑1 (MQ=255)                                      ggCAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGaa      <  1:186299/50‑1 (MQ=255)                                        cAAGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGaa      >  2:285341/1‑48 (MQ=255)                                          aGTTCAGACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGAACAGa  <  2:1094341/50‑1 (MQ=255)                                                |                                            GATTTTGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAAGTTCACACCGATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGAACAGA  >  NC_017635/4729622‑4729712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑