Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	1,437,357	C→A	V315F (GTT→TTT)	yciW ←	CMD domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	1,437,357	0	C	A	100.0%	162.4 / NA	41	V315F (GTT→TTT)	yciW	CMD domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/15);  total (26/15)

CGGGTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTG  >  NC_017635/1437317‑1437400                                         |                                            cGGGTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACg                                    >  2:25821/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  1:136351/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  2:633869/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  2:1873129/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  1:1579553/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  2:149034/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  1:207841/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  1:919461/1‑50 (MQ=255)  gggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACgg                                   >  1:410980/1‑50 (MQ=255)   ggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGt                                  >  2:919327/1‑50 (MQ=255)   ggTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGt                                  <  2:102620/50‑1 (MQ=255)    gTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGt                                  >  1:90599/1‑49 (MQ=255)    gTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTc                                 <  2:1829633/50‑1 (MQ=255)     tCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCa                                <  1:884883/50‑1 (MQ=255)      cAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAg                               >  1:2079551/1‑50 (MQ=255)      cAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAg                               >  1:1116616/1‑50 (MQ=255)        gCCATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGcc                             <  1:223724/50‑1 (MQ=255)         ccATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGccc                            <  1:1147941/50‑1 (MQ=255)         ccATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGccc                            <  1:1364125/50‑1 (MQ=255)         ccATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGccc                            <  1:1899438/50‑1 (MQ=255)         ccATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGccc                            <  1:774878/50‑1 (MQ=255)          cATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGCCGGTCAGCCCa                           >  2:523828/1‑50 (MQ=255)          cATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCa                           >  2:1005054/1‑50 (MQ=255)          cATTGTATCGCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCa                           >  1:1405125/1‑50 (MQ=255)                   gCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAAt                  <  1:1899399/50‑1 (MQ=255)                   gCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAAt                  >  2:159192/1‑50 (MQ=255)                   gCCTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAAt                  >  1:766184/1‑50 (MQ=255)                     cTGAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAAttt                <  2:237679/50‑1 (MQ=255)                       gAACGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCa              >  2:1002483/1‑50 (MQ=255)                        aaCGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTc               <  2:1248676/48‑1 (MQ=255)                        aaCGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCAt             <  1:806963/50‑1 (MQ=255)                        aaCGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCAt             >  2:1701090/1‑50 (MQ=255)                         aCGGTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCAtt            <  1:1420067/50‑1 (MQ=255)                            gTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTc         >  2:1503045/1‑50 (MQ=255)                            gTAGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTc         >  2:2061362/1‑50 (MQ=255)                              aGCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGc       >  2:1513540/1‑50 (MQ=255)                               gCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGcc      <  1:871924/50‑1 (MQ=255)                               gCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGcc      >  2:1018274/1‑50 (MQ=255)                               gCGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGcc      >  2:1012610/1‑50 (MQ=255)                                cGTGCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGccc     <  1:706020/50‑1 (MQ=255)                                   gCTCAAAAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTg  >  1:238571/1‑50 (MQ=255)                                         |                                            CGGGTCAGCCATTGTATCGCCTGAACGGTAGCGTGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTG  >  NC_017635/1437317‑1437400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑