Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_017635	2,322,414	A→G	V93V (GTT→GTC)	ECW_RS11650 ←	head completion/stabilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_017635	2,322,414	0	A	G	100.0%	55.5 / NA	25	V93V (GTT→GTC)	ECW_RS11650	head completion/stabilization protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/5);  total (20/5)

AGTAAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGAACGTCCGCCAGACGCGAAAAACCGGCAGCAATTTTCTGTT  >  NC_017635/2322371‑2322454                                            |                                         agtaAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGt                                      >  1:1014585/1‑48 (MQ=255) agtaAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGt                                      >  2:374916/1‑48 (MQ=255) agtaAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGt                                      >  2:2469949/1‑48 (MQ=255) agtaAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGt                                      >  1:21197/1‑48 (MQ=255)   taAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGt                                      >  2:947992/1‑46 (MQ=255)    aaaaaaCTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGTcag                                   >  1:1393384/1‑46 (MQ=11)    aaaaaaCTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGTcag                                   >  1:1440410/1‑46 (MQ=11)    aaaaaaCTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGTcag                                   >  1:1639563/1‑46 (MQ=11)    aaaaaaCTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGTcag                                   >  1:2554947/1‑46 (MQ=11)      aaaaCTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGGACGTCAGcc                                 <  1:1361529/48‑1 (MQ=11)               aTGCTTTCACCGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGa                         >  2:1382313/1‑47 (MQ=14)                 gCTTTCACCGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGaaa                       >  2:34043/1‑47 (MQ=14)                      cACCGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCgg                 >  1:2606281/1‑48 (MQ=14)                        ccGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCgg                 >  2:1671655/1‑46 (MQ=14)                        ccGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGgca               <  2:1674748/48‑1 (MQ=17)                         cGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCgg                 >  1:1715300/1‑45 (MQ=14)                         cGTCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGgcag              >  2:918583/1‑48 (MQ=17)                           tCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGgcagca            <  1:2411054/48‑1 (MQ=17)                           tCGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGgcagca            <  2:1823767/48‑1 (MQ=17)                            cGATGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGGCAGCaa           >  1:1419068/1‑48 (MQ=17)                               tGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGGCAGCAAttt        <  1:454391/48‑1 (MQ=17)                               tGCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGGCAGCAAttt        >  2:52116/1‑48 (MQ=17)                                gCCGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGGCAGCAAtttt       >  1:645738/1‑48 (MQ=17)                                  cGTCCGCCGGGACGTCAGCCAGACGCGAAAAACCGGCAGCAATTTTCt     >  2:582181/1‑48 (MQ=14)                                     ccgccgGGACGTCAGCCAGACGCGAAAAACCGGCAGCAATTTTCTGtt  >  1:2047140/1‑48 (MQ=14)                                            |                                         AGTAAAAAACTTTGATGCTTTCACCGTCGATGCCGTCCGCCGGAACGTCCGCCAGACGCGAAAAACCGGCAGCAATTTTCTGTT  >  NC_017635/2322371‑2322454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑