breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 119,500 | A→C | intergenic (+157/‑4) | pdhR → / → aceE | pyruvate dehydrogenase complex transcriptional repressor PdhR/pyruvate dehydrogenase (acetyl‑transferring), homodimeric type |
MC JC | 1,290,924 | 2651 bp→80 bp | coding (4‑2654/2676 nt) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
MC JC | 1,749,959 | 1388 bp→80 bp | coding (5‑1392/1413 nt) | pykF → | pyruvate kinase PykF |
MC JC | 1,931,909 | 1419 bp→81 bp | coding (4‑1422/1443 nt) | pyk → | pyruvate kinase |
RA | 3,081,838 | G→A | A66T (GCA→ACA) | galP → | galactose/proton symporter |
RA | 4,140,541 | A→C | I829S (ATT→AGT) | ppc ← | phosphoenolpyruvate carboxylase |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_CP009273 | 578586 | 579163 | 578 | 41 [34] | [33] 37 | [BW25113_RS26085]–[appY] | [BW25113_RS26085],[appY] |
* | * | ÷ | NZ_CP009273 | 946682 | 949071 | 2390 | 44 [36] | [36] 38 | pflB–[focA] | pflB,[focA] |
* | * | ÷ | NZ_CP009273 | 2185663 | 2185982 | 320 | 37 [36] | [36] 37 | [yehD] | [yehD] |
* | * | ÷ | NZ_CP009273 | 2462582 | 2463372 | 791 | 37 [36] | [32] 37 | [yfdH]–[BW25113_RS12270] | [yfdH],[BW25113_RS12270] |
* | * | ÷ | NZ_CP009273 | 2897025 | 2897368 | 344 | 40 [36] | [36] 37 | ygcE/queE | FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE |
* | * | ÷ | NZ_CP009273 | 3647046 | 3647305 | 260 | 39 [35] | [35] 37 | BW25113_RS18165/slp | hypothetical protein/outer membrane lipoprotein Slp |
* | * | ÷ | NZ_CP009273 | 3797547 | 3798549 | 1003 | 38 [35] | [29] 38 | [rfaS]–[rfaP] | [rfaS],[rfaP] |
* | * | ÷ | NZ_CP009273 | 4127811 | 4128990 | 1180 | 37 [36] | [36] 37 | gldA–[fsa] | gldA,[fsa] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP009273 | 360104 = | 39 (0.410) | 57 (0.600) | 47/480 | 1.0 | 58.5% | intergenic (‑35/+251) | lacZ/BW25113_RS23320 | beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein |
? | NZ_CP009273 | = 360286 | 42 (0.450) | intergenic (‑217/+69) | lacZ/BW25113_RS23320 | beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein | |||||
* | ? | NZ_CP009273 | 1972967 = | 0 (0.000) | 44 (0.470) | 30/480 | 2.7 | 100% | intergenic (‑513/‑267) | flhD/uspC | flagellar transcriptional regulator FlhD/universal stress protein UspC |
? | NZ_CP009273 | 2095230 = | NA (NA) | pseudogene (1/354 nt) | wbbL | N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL | |||||
* | ? | NZ_CP009273 | = 1972970 | 0 (0.000) | 88 (0.930) | 64/480 | 0.3 | 100% | intergenic (‑516/‑264) | flhD/uspC | flagellar transcriptional regulator FlhD/universal stress protein UspC |
? | NZ_CP009273 | = 2283592 | NA (NA) | intergenic (‑68/‑387) | BW25113_RS11435/narP | IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP |