breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 119,500 A→C intergenic (+157/‑4) pdhR → / → aceE pyruvate dehydrogenase complex transcriptional repressor PdhR/pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
MC JC 1,290,924 2651 bp→80 bp coding (4‑2654/2676 nt) adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
MC JC 1,749,959 1388 bp→80 bp coding (5‑1392/1413 nt) pykF → pyruvate kinase PykF
MC JC 1,931,909 1419 bp→81 bp coding (4‑1422/1443 nt) pyk → pyruvate kinase
RA 3,081,838 G→A A66T (GCA→ACA)  galP → galactose/proton symporter
RA 4,140,541 A→C I829S (ATT→AGT)  ppc ← phosphoenolpyruvate carboxylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 578586 579163 578 41 [34] [33] 37 [BW25113_RS26085]–[appY] [BW25113_RS26085],[appY]
* * ÷ NZ_CP009273 946682 949071 2390 44 [36] [36] 38 pflB–[focA] pflB,[focA]
* * ÷ NZ_CP009273 2185663 2185982 320 37 [36] [36] 37 [yehD] [yehD]
* * ÷ NZ_CP009273 2462582 2463372 791 37 [36] [32] 37 [yfdH]–[BW25113_RS12270] [yfdH],[BW25113_RS12270]
* * ÷ NZ_CP009273 2897025 2897368 344 40 [36] [36] 37 ygcE/queE FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
* * ÷ NZ_CP009273 3647046 3647305 260 39 [35] [35] 37 BW25113_RS18165/slp hypothetical protein/outer membrane lipoprotein Slp
* * ÷ NZ_CP009273 3797547 3798549 1003 38 [35] [29] 38 [rfaS]–[rfaP] [rfaS],[rfaP]
* * ÷ NZ_CP009273 4127811 4128990 1180 37 [36] [36] 37 gldA–[fsa] gldA,[fsa]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =39 (0.410)57 (0.600) 47/480 1.0 58.5% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 42 (0.450)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1972967 =0 (0.000)44 (0.470) 30/480 2.7 100% intergenic (‑513/‑267) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 2095230 = NA (NA)pseudogene (1/354 nt) wbbL N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL
* ? NZ_CP009273 = 19729700 (0.000)88 (0.930) 64/480 0.3 100% intergenic (‑516/‑264) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP