BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_009004	1,532,424	G→C	P159R (CCC→CGC)	LLMG_RS07840 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_009004	1,532,424	0	G	C	99.4%	2000.0 / ‑5.5	523	P159R (CCC→CGC)	LLMG_RS07840	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/3); new base C (257/263); total (257/266)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 523 total aligned reads displayed.

GCAAGCTCGTCTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGGGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCAATGCTT  >  NC_009004/1532279‑1532568

 cAAGCCCGTCTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGaa                                                                                                                                               <  1:11129970/148‑1 (MQ=255)

  aaGCCCGTCTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGaat                                                                                                                                              <  1:1976312/148‑1 (MQ=255)

   aGCTCGTCTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGaata                                                                                                                                             <  1:10926198/148‑1 (MQ=255)

       cGTCTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAg                                                                                                                                         <  1:5452570/148‑1 (MQ=255)

          cTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACa                                                                                                                                      >  1:9309778/1‑148 (MQ=255)

          cTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTATAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACa                                                                                                                                      >  1:274513/1‑148 (MQ=255)

           tGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACaa                                                                                                                                     <  1:7802298/148‑1 (MQ=255)

             gACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAAAAGACAAAt                                                                                                                                   >  1:6250957/1‑148 (MQ=255)

             gACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTCTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCTAATAATAGTCaaac                                                                                                                                   >  1:11202210/1‑147 (MQ=255)

              aCTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAAtt                                                                                                                                  >  1:833205/1‑148 (MQ=255)

              aCTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAAtt                                                                                                                                  >  1:7469238/1‑148 (MQ=255)

                 cAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATtata                                                                                                                               >  1:575254/1‑148 (MQ=255)

                  aaGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGACACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAg                                                                                                                              <  1:7532787/148‑1 (MQ=255)

                  aaGTCAGAAAAAACTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAg                                                                                                                              >  1:2399650/1‑148 (MQ=255)

                   aGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGc                                                                                                                             >  1:10540330/1‑148 (MQ=255)

                       aGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTa                                                                                                                         >  1:4643194/1‑148 (MQ=255)

                       aGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTa                                                                                                                         >  1:2655723/1‑148 (MQ=255)

                       aGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTa                                                                                                                         >  1:1071241/1‑148 (MQ=255)

                               gTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGc                                                                                                                 >  1:10650987/1‑148 (MQ=255)

                                 gggACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGa                                                                                                               <  1:3630224/148‑1 (MQ=255)

                                  ggACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGaa                                                                                                              <  1:7667826/148‑1 (MQ=255)

                                    aCTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAAtt                                                                                                            <  1:354816/148‑1 (MQ=255)

                                      tAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGc                                                                                                          <  1:8522563/148‑1 (MQ=255)

                                         cTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCACCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAg                                                                                                       <  1:10493474/148‑1 (MQ=255)

                                           ggATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGtt                                                                                                     <  1:11334232/148‑1 (MQ=255)

                                            gATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTc                                                                                                    >  1:876580/1‑148 (MQ=255)

                                            gATACAATGGTGGTCACTTAAATGCTCATCAAAATCATTAATCTTCATCTTCCTCCACAATAAATTCGCCTATCGTATTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGAAAAGCTTGCGAATTGCAAGTTc                                                                                                    >  1:8130366/1‑148 (MQ=255)

                                              tACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTa                                                                                                  <  1:8440019/148‑1 (MQ=255)

                                               aCAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTaa                                                                                                 <  1:9783971/148‑1 (MQ=255)

                                                cAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAg                                                                                                <  1:4929997/148‑1 (MQ=255)

                                                cAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAg                                                                                                <  1:8254167/148‑1 (MQ=255)

                                                 aaTGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCGTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGc                                                                                               <  1:6096395/148‑1 (MQ=255)

                                                 aaTGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGc                                                                                               <  1:10879323/148‑1 (MQ=255)

                                                   tggtggTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTa                                                                                             <  1:3936249/148‑1 (MQ=255)

                                                     gtggtCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTaaa                                                                                           >  1:4046156/1‑148 (MQ=255)

                                                     gtggtCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTaaa                                                                                           <  1:11040946/148‑1 (MQ=255)

                                                      tggtCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAAt                                                                                          <  1:4827022/148‑1 (MQ=255)

                                                        gtCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAAttt                                                                                        <  1:10319517/148‑1 (MQ=255)

                                                            cTTAACTGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTGCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTttct                                                                                    >  1:280314/1‑148 (MQ=255)

                                                             ttAAATGCTCCTCAAAATCTTTAACCTTCATCTTCCTCCTCAATAAATTCGCCTACCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAACTGCAAGTTCTAAGCTAAATTTttctt                                                                                   >  1:9191100/1‑148 (MQ=255)

                                                             ttAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTttctt                                                                                   >  1:10406285/1‑148 (MQ=255)

                                                              tAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTttcttc                                                                                  <  1:6507484/148‑1 (MQ=255)

                                                                aaTGCTCATCAAAATCTTAAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCt                                                                                >  1:58714/1‑148 (MQ=255)

                                                                   gCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCtttt                                                                             <  1:11236279/148‑1 (MQ=255)

                                                                   gCTCATCAAAATCTTTAATCTACATCTTCCTTCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCtttt                                                                             <  1:6770068/148‑1 (MQ=255)

                                                                     tcatcaAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTgc                                                                           >  1:7292296/1‑148 (MQ=255)

                                                                        tcaAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCt                                                                        <  1:9920908/148‑1 (MQ=255)

                                                                        tcaAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCt                                                                        <  1:10772552/148‑1 (MQ=255)

                                                                           aaaTCTTTAATCTTCATCATCCTCCTCAGTAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGt                                                                     <  1:7584223/148‑1 (MQ=255)

                                                                              tCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTa                                                                  >  1:3871282/1‑148 (MQ=255)

                                                                               cTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTaa                                                                 <  1:9437249/148‑1 (MQ=255)

                                                                               cTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTaa                                                                 <  1:6921324/148‑1 (MQ=255)

                                                                               cTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTaa                                                                 <  1:7100251/148‑1 (MQ=255)

                                                                                   aaTCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTTCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTTAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGc                                                             >  1:455639/1‑148 (MQ=255)

                                                                                     tcttcatcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTc                                                           <  1:8044421/148‑1 (MQ=255)

                                                                                     tcttcatcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTc                                                           >  1:8601676/1‑148 (MQ=255)

                                                                                     tcttcatcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTc                                                           >  1:7196229/1‑148 (MQ=255)

                                                                                     tcttcatcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTc                                                           >  1:1467171/1‑148 (MQ=255)

                                                                                      cttcatcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCg                                                          >  1:8941979/1‑148 (MQ=255)

                                                                                        tcatcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTc                                                        <  1:10015088/148‑1 (MQ=255)

                                                                                          atcttcCTCCTCAATCAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCg                                                      <  1:5257083/148‑1 (MQ=255)

                                                                                          atcttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCg                                                      <  1:119744/148‑1 (MQ=255)

                                                                                             ttcCTCCTCAATATATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGata                                                   <  1:3059520/148‑1 (MQ=255)

                                                                                             ttcCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGata                                                   <  1:3177699/148‑1 (MQ=255)

                                                                                               cctcctCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGaagtc                                                 >  1:4129804/1‑144 (MQ=255)

                                                                                                  cgtcAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGataaataa                                              >  1:3801035/3‑148 (MQ=255)

                                                                                                  cctcAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGataaataa                                              >  1:9963370/1‑148 (MQ=255)

                                                                                                    tcAATAAATTCGCCTTTCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCATCCTTTTGCGCTAGTTTAATCGCTCGTCCGATaaataaat                                            <  1:1167515/148‑1 (MQ=255)

                                                                                                      aaTAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAAttt                                          >  1:4290187/1‑148 (MQ=255)

                                                                                                      aaTAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAAttt                                          <  1:9660759/148‑1 (MQ=255)

                                                                                                        tAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTATGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTg                                        <  1:1773593/148‑1 (MQ=255)

                                                                                                         aaaTTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTgg                                       >  1:5970634/1‑148 (MQ=255)

                                                                                                           aTTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATACCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGtt                                     >  1:1295566/1‑148 (MQ=255)

                                                                                                            ttCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTc                                    >  1:7894028/1‑148 (MQ=255)

                                                                                                                ccTATCGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGaa                                >  1:2886755/1‑148 (MQ=255)

                                                                                                                     cGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCa                           <  1:5071709/148‑1 (MQ=255)

                                                                                                                     cGTTTTTCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCa                           >  1:2074754/1‑148 (MQ=255)

                                                                                                                       tttttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCAtt                         >  1:11496944/1‑148 (MQ=255)

                                                                                                                        ttttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGGATTGCAAGTCCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTg                        <  1:6716077/148‑1 (MQ=255)

                                                                                                                        ttttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTg                        >  1:4427/1‑148 (MQ=255)

                                                                                                                        ttttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTg                        <  1:10833653/148‑1 (MQ=255)

                                                                                                                        ttttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTg                        >  1:1494197/1‑148 (MQ=255)

                                                                                                                        ttttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTg                        <  1:2475536/148‑1 (MQ=255)

                                                                                                                          ttCCAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGAt                      >  1:10100688/1‑148 (MQ=255)

                                                                                                                             cAAGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATaaa                   >  1:1376781/1‑148 (MQ=255)

                                                                                                                              aaGTTCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAg                  >  1:343184/1‑148 (MQ=255)

                                                                                                                               aGTTCTCCTGAAAAATAGCGAATAATAGACAAATTATAGTCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTGCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGt                 <  1:907085/148‑1 (MQ=255)

                                                                                                                                gTTCTCCTGCAAAATAGCGAATAATAGACAGATTATAGCCGTAAAGCTTGCGGATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATTGCTCGTCCGATAAATGAATTTTGGTTCCGAAAATCATTGATAAAGtt                <  1:11404604/148‑1 (MQ=255)

                                                                                                                                 ttCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGttt               <  1:7033146/148‑1 (MQ=255)

                                                                                                                                 ttCTCCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGttt               <  1:3344105/148‑1 (MQ=255)

                                                                                                                                  tctcCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGtttt              >  1:2202670/1‑148 (MQ=255)

                                                                                                                                   ctcCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTc             >  1:6853050/1‑148 (MQ=255)

                                                                                                                                    tcCTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCt            <  1:5590791/148‑1 (MQ=255)

                                                                                                                                      cTGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTACTGAAAATCATTGATAAAGTTTTCTAt          <  1:294585/148‑1 (MQ=255)

                                                                                                                                       tGCAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGTTAAAGTTTTCTATc         >  1:9567904/1‑148 (MQ=255)

                                                                                                                                         cAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCaa       <  1:8865828/148‑1 (MQ=255)

                                                                                                                                         cAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCaa       >  1:9522538/1‑148 (MQ=255)

                                                                                                                                         cAAAATAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCaa       <  1:6351990/148‑1 (MQ=255)

                                                                                                                                           aaaTAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCAATg     >  1:1616369/1‑148 (MQ=255)

                                                                                                                                             aTAGCGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCAATGCt   <  1:10242927/148‑1 (MQ=255)

GCAAGCTCGTCTGGACTCAAGTCAGAAAAAAGTGGGACTAGCTGGATACAATGGTGGTCACTTAAATGCTCATCAAAATCTTTAATCTTCATCTTCCTCCTCAATAAATTCGCCTATCGTTTTTCCAAGTTCTCCTGCAAAATAGGGAATAATAGACAAATTATAGCCGTAAAGCTTGCGAATTGCAAGTTCTAAGCTAAATTTTTCTTCCTTTTGCGCTAGTTTAATCGCTCGTCCGATAAATAAATTTTGGTTCTGAAAATCATTGATAAAGTTTTCTATCAATGCTT  >  NC_009004/1532279‑1532568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`