BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_009004	1,588,245	2 bp→AT	coding (837‑838/1170 nt)	LLMG_RS08135 ←	nucleotide sugar dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_009004	1,588,245	0	T	A	99.4%	1976.2 / NA	519	T280S (ACA→TCA)	LLMG_RS08135	nucleotide sugar dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (3/0); new base A (288/226); total (292/227)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_009004	1,588,246	0	A	T	100.0%	2007.8 / NA	519	N279K (AAT→AAA)	LLMG_RS08135	nucleotide sugar dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (292/227); total (292/227)

Only 100 of 523 total aligned reads displayed.

TGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACA  >  NC_009004/1588103‑1588387

||

  cAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACa                                                                                                                                         >  1:11253719/1‑148 (MQ=255)

   aaGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACaa                                                                                                                                        >  1:4519523/1‑148 (MQ=255)

      agTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAg                                                                                                                                     >  1:10375605/1‑148 (MQ=255)

      agTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAg                                                                                                                                     >  1:10630388/1‑148 (MQ=255)

       gTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGc                                                                                                                                    <  1:11162222/148‑1 (MQ=255)

        tttCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGcc                                                                                                                                   >  1:765531/1‑148 (MQ=255)

         ttCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCg                                                                                                                                  >  1:10792535/1‑148 (MQ=255)

         ttCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCg                                                                                                                                  >  1:5457047/1‑148 (MQ=255)

            atcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                                                                               <  1:6493084/148‑1 (MQ=255)

            atcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                                                                               <  1:3104439/148‑1 (MQ=255)

             tcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcat                                                                                                                              <  1:5874162/148‑1 (MQ=255)

                catcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCaa                                                                                                                           >  1:2865893/1‑148 (MQ=255)

                    aataatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGGCGTCATCAACTcc                                                                                                                       <  1:5022126/148‑1 (MQ=255)

                    aataatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTcc                                                                                                                       <  1:11680816/148‑1 (MQ=255)

                      taatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTg                                                                                                                     >  1:11386316/1‑148 (MQ=255)

                         tCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAgg                                                                                                                  <  1:674471/148‑1 (MQ=255)

                          cGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGa                                                                                                                 <  1:1773891/148‑1 (MQ=255)

                           gAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGaa                                                                                                                >  1:8242011/1‑148 (MQ=255)

                               tctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGcc                                                                                                            >  1:6295939/1‑148 (MQ=255)

                                  gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATATTCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTc                                                                                                         <  1:2488414/148‑1 (MQ=255)

                                  gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTc                                                                                                         <  1:1699323/148‑1 (MQ=255)

                                  gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTc                                                                                                         >  1:6060247/1‑148 (MQ=255)

                                  gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTc                                                                                                         <  1:720095/148‑1 (MQ=255)

                                   tctgAAATTATCTGATCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCa                                                                                                        >  1:42291/1‑148 (MQ=255)

                                    ctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCAt                                                                                                       >  1:3487410/1‑148 (MQ=255)

                                     tgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATa                                                                                                      >  1:8761918/1‑148 (MQ=255)

                                       aaaTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTGCTGAGGAACGCCCTCATAAc                                                                                                    <  1:1456964/148‑1 (MQ=255)

                                            aTCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGTCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGATCGCCCTCATAACTGGcc                                                                                               <  1:481832/148‑1 (MQ=255)

                                            aTCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGcc                                                                                               >  1:11193613/1‑148 (MQ=255)

                                              cTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCaa                                                                                             <  1:296988/148‑1 (MQ=255)

                                               tGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCaaa                                                                                            >  1:7868970/1‑148 (MQ=255)

                                                gccccAGCTCTCATGACCACCCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGTACGCCCTCATAACTGGCCaaaa                                                                                           <  1:7585529/146‑1 (MQ=255)

                                                gACCCAGCTTTCATGACCAACCCGCAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTGTGATTTCACAATAGCCGTCATCAACTCCTGTGGAACGCCCTCATAACTGGCCaaaa                                                                                           >  1:9428856/1‑148 (MQ=255)

                                                  cccAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgc                                                                                         >  1:7033525/1‑148 (MQ=255)

                                                       cTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCtt                                                                                    >  1:4431488/1‑148 (MQ=255)

                                                        tttCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCttt                                                                                   <  1:1955577/148‑1 (MQ=255)

                                                          tCATGACTAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGa                                                                                 >  1:2911647/1‑148 (MQ=255)

                                                          tCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTTTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGa                                                                                 >  1:2744236/1‑148 (MQ=255)

                                                            aTGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGt                                                                               <  1:2692068/148‑1 (MQ=255)

                                                              gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCt                                                                             >  1:7430422/1‑148 (MQ=255)

                                                               aCCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                                                                            <  1:9282037/148‑1 (MQ=255)

                                                                ccAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAATGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCttt                                                                           <  1:2020317/148‑1 (MQ=255)

                                                                  aaCCGGTAAATTCCACCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCg                                                                         <  1:5605544/148‑1 (MQ=255)

                                                                         aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACa                                                                  >  1:3357876/1‑148 (MQ=255)

                                                                         aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACa                                                                  >  1:8512956/1‑148 (MQ=255)

                                                                          aaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACaa                                                                 <  1:8313862/148‑1 (MQ=255)

                                                                                aaCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAacca                                                           >  1:9494448/1‑148 (MQ=255)

                                                                                 aCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAaccac                                                          <  1:3960532/148‑1 (MQ=255)

                                                                                   aGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAAccacca                                                        >  1:9660237/1‑148 (MQ=255)

                                                                                    gTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCAt                                                       >  1:10003698/1‑148 (MQ=255)

                                                                                    gTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCAt                                                       >  1:10216360/1‑148 (MQ=255)

                                                                                     tttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATa                                                      <  1:5360662/148‑1 (MQ=255)

                                                                                     tttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATa                                                      <  1:7148116/148‑1 (MQ=255)

                                                                                     tttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATa                                                      >  1:5106938/1‑148 (MQ=255)

                                                                                      ttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATaa                                                     <  1:1610132/148‑1 (MQ=255)

                                                                                          ggagaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCaa                                                 >  1:1042636/2‑148 (MQ=255)

                                                                                           gagaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCaaa                                                >  1:6863321/1‑148 (MQ=255)

                                                                                            acaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCaaaa                                               <  1:1352071/146‑1 (MQ=255)

                                                                                             gaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAAc                                              <  1:4165650/148‑1 (MQ=255)

                                                                                              aTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCACCTCCTGAGCAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACt                                             >  1:7969791/1‑148 (MQ=255)

                                                                                                 tAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTgg                                          >  1:4669164/1‑148 (MQ=255)

                                                                                                 tAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTgg                                          >  1:4312041/1‑148 (MQ=255)

                                                                                                    ccATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGTACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAACACTTGGact                                       >  1:8371849/1‑146 (MQ=255)

                                                                                                    ccATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGatt                                       >  1:10171520/1‑148 (MQ=255)

                                                                                                    ccATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGatt                                       >  1:2213330/1‑148 (MQ=255)

                                                                                                     cATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGatta                                      <  1:3266842/148‑1 (MQ=255)

                                                                                                     cATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATGTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGGGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGatta                                      >  1:1225069/1‑148 (MQ=255)

                                                                                                      aTAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattat                                     >  1:8118776/1‑148 (MQ=255)

                                                                                                       tAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatt                                    >  1:2425204/1‑148 (MQ=255)

                                                                                                        aaTCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatta                                   <  1:6621931/148‑1 (MQ=255)

                                                                                                        aaTCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatta                                   >  1:2293200/1‑148 (MQ=255)

                                                                                                        aaTCTGCTCAACAATGTGCTGTATTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatta                                   >  1:7752763/1‑148 (MQ=255)

                                                                                                         aTCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattattat                                  >  1:2117836/1‑148 (MQ=255)

                                                                                                             gCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtg                              >  1:7816972/1‑148 (MQ=255)

                                                                                                              cTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtgt                             >  1:3669300/1‑148 (MQ=255)

                                                                                                                caacaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATgtgt                           >  1:11622221/1‑148 (MQ=255)

                                                                                                                 aacaaTGTGTTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTc                          <  1:6358604/148‑1 (MQ=255)

                                                                                                                   caaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTccc                        >  1:8890034/1‑148 (MQ=255)

                                                                                                                    aaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAAGGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGGAAACAATAACCAGCATAACCAAAACTTGGATTATTATAATGTGTCGCa                       <  1:4936138/148‑1 (MQ=255)

                                                                                                                     aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGTCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCaa                      >  1:7334235/1‑148 (MQ=255)

                                                                                                                     aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCaa                      >  1:4730571/1‑148 (MQ=255)

                                                                                                                      tgtgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAAt                     <  1:8600637/148‑1 (MQ=255)

                                                                                                                       gtgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATc                    >  1:9151376/1‑148 (MQ=255)

                                                                                                                       gtgCTGTTTTCTGGTTTCGTTTGATTTGACAATACCCGTCATCAACTCCTGAGGAACGCCCACATAACTGGCCATAAGCTGCTTTGAGTCTGTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATc                    <  1:619218/148‑1 (MQ=255)

                                                                                                                        tgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATcc                   >  1:8626208/1‑148 (MQ=255)

                                                                                                                          cTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGt                 >  1:5982012/1‑148 (MQ=255)

                                                                                                                             ttttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGaa              >  1:750319/1‑148 (MQ=255)

                                                                                                                             ttttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGaa              >  1:11053468/1‑148 (MQ=255)

                                                                                                                               ttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATc            >  1:10971181/1‑148 (MQ=255)

                                                                                                                                tCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcc           <  1:5280151/148‑1 (MQ=255)

                                                                                                                                tCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcc           >  1:5716487/1‑148 (MQ=255)

                                                                                                                                tCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcc           >  1:9777707/1‑148 (MQ=255)

                                                                                                                                 cTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcca          <  1:902914/148‑1 (MQ=255)

                                                                                                                                  tGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaa         >  1:8706704/1‑148 (MQ=255)

                                                                                                                                   ggTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaac        <  1:2574016/148‑1 (MQ=255)

                                                                                                                                    gTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacc       >  1:11477906/1‑148 (MQ=255)

                                                                                                                                     tttCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaaccc      <  1:5775437/148‑1 (MQ=255)

                                                                                                                                      ttCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaaccca     <  1:3574115/148‑1 (MQ=255)

                                                                                                                                      ttCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaaccca     >  1:6132664/1‑148 (MQ=255)

                                                                                                                                       tCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacccaa    >  1:3833534/1‑148 (MQ=255)

||

TGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACA  >  NC_009004/1588103‑1588387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`