BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_009004	636,592	(A)_7→6	coding (297/1164 nt)	LLMG_RS03345 ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_009004	636,586	0	A	.	99.7%	3261.4 / 2.1	699	coding (303/1164 nt)	LLMG_RS03345	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (343/353); total (344/355)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 745 total aligned reads displayed.

AGTTTTACCGATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCAAAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAGGGACGATAAA  >  NC_009004/636439‑636729

 gTTTTACCGATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCaa                                                                                                                                                >  1:9607215/1‑148 (MQ=255)

   tttACCGATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCaaaa                                                                                                                                              <  1:1277240/148‑1 (MQ=255)

   tttACCGATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCaaaa                                                                                                                                              <  1:10697158/148‑1 (MQ=255)

     tACCGATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCaaaaaa                                                                                                                                            <  1:8347641/148‑1 (MQ=255)

         gATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCaaaaaaccta                                                                                                                                        >  1:10435447/1‑144 (MQ=255)

          ataataTACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCtaa                                                                                                                                      <  1:10097212/148‑1 (MQ=255)

          ataataTACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCtaa                                                                                                                                      <  1:7459215/148‑1 (MQ=255)

            aataTACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCtaata                                                                                                                                    >  1:2173742/1‑148 (MQ=255)

            aagataCCAGAAAGTAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCAAAAAAA‑Ctaata                                                                                                                                    <  1:5274299/145‑1 (MQ=255)

              tataCCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTaataat                                                                                                                                  >  1:11678944/1‑148 (MQ=255)

                    aGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCgag                                                                                                                            <  1:3059294/148‑1 (MQ=255)

                     gAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCgaga                                                                                                                           <  1:2950888/148‑1 (MQ=255)

                     gAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCgaga                                                                                                                           >  1:9447734/1‑148 (MQ=255)

                       aaaTAATCTGAACGTCAAATGCAATACTGGTGTAGACTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAgg                                                                                                                         >  1:4578733/1‑148 (MQ=255)

                       aaaTAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAgg                                                                                                                         <  1:6512370/148‑1 (MQ=255)

                         ataatCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGAt                                                                                                                       >  1:7886335/1‑148 (MQ=255)

                          taatCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGAtt                                                                                                                      >  1:8917653/1‑148 (MQ=255)

                          taatCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGAtt                                                                                                                      >  1:3313292/1‑148 (MQ=255)

                          taatCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGAtt                                                                                                                      <  1:9210987/148‑1 (MQ=255)

                            atCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATtat                                                                                                                    >  1:4430313/1‑148 (MQ=255)

                            atCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATtat                                                                                                                    <  1:5120462/148‑1 (MQ=255)

                             tCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATtata                                                                                                                   <  1:6985073/148‑1 (MQ=255)

                               tGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACt                                                                                                                 <  1:5911578/148‑1 (MQ=255)

                               tGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACt                                                                                                                 <  1:6391261/148‑1 (MQ=255)

                                gAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACtt                                                                                                                >  1:471445/1‑148 (MQ=255)

                                 aaCGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTa                                                                                                               <  1:5063231/148‑1 (MQ=255)

                                 aaCGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAATAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTa                                                                                                               >  1:7162181/1‑148 (MQ=255)

                                  aCGGCAGATGCACTACTGGTGTAGGCTCCTGATAATGTTCAGCCTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTACATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTag                                                                                                              <  1:6313944/148‑2 (MQ=255)

                                  aCGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTaa                                                                                                              >  1:8147701/1‑148 (MQ=255)

                                  aCGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTaa                                                                                                              <  1:10369724/148‑1 (MQ=255)

                                   cGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTaaa                                                                                                             <  1:11577617/148‑1 (MQ=255)

                                   cGGCAAATGCAATACTGGTGTAGCCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTaaa                                                                                                             >  1:10881820/1‑148 (MQ=255)

                                    ggCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAAc                                                                                                            <  1:7766864/148‑1 (MQ=255)

                                     gCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACt                                                                                                           <  1:8678254/148‑1 (MQ=255)

                                       aaaTGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTg                                                                                                         <  1:6637589/148‑1 (MQ=255)

                                        taTGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGc                                                                                                        <  1:4652581/147‑1 (MQ=255)

                                        aaTGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGc                                                                                                        >  1:7576548/1‑148 (MQ=255)

                                         aTGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCt                                                                                                       >  1:2360645/1‑148 (MQ=255)

                                            cAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACt                                                                                                    <  1:10225117/148‑1 (MQ=255)

                                             aaTACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTg                                                                                                   <  1:4517131/148‑1 (MQ=255)

                                              aTACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGa                                                                                                  >  1:3425202/1‑148 (MQ=255)

                                               tACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGAc                                                                                                 >  1:1404384/1‑148 (MQ=255)

                                               tACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGAc                                                                                                 <  1:10562461/148‑1 (MQ=255)

                                                  tGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATGTAGATTCTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGTAACC‑AAAAAACCTAATACTGTCGAGGGGATTATACTCAAACTTGCTACTGGCAgg                                                                                              >  1:7333368/1‑148 (MQ=255)

                                                    gtgtAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTg                                                                                            >  1:8272950/1‑148 (MQ=255)

                                                    gtgtAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTg                                                                                            <  1:4943645/148‑1 (MQ=255)

                                                       tAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCg                                                                                         <  1:10819698/148‑1 (MQ=255)

                                                        aGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGa                                                                                        <  1:9794758/148‑1 (MQ=255)

                                                          gCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACt                                                                                      <  1:674074/148‑1 (MQ=255)

                                                              cTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCa                                                                                  <  1:10026578/148‑1 (MQ=255)

                                                               tGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAAGTTGCTACTGACAGGTGCCGACTTCCAg                                                                                 >  1:906698/1‑148 (MQ=255)

                                                                gATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGt                                                                                <  1:11282579/148‑1 (MQ=255)

                                                                 aTAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTa                                                                               <  1:2074601/148‑1 (MQ=255)

                                                                  tAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTaa                                                                              >  1:11382857/1‑148 (MQ=255)

                                                                       ttAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCa                                                                         <  1:2816881/148‑1 (MQ=255)

                                                                           gCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAAttt                                                                     >  1:8090404/1‑148 (MQ=255)

                                                                              ttCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTgag                                                                  >  1:412786/1‑148 (MQ=255)

                                                                              ttCTCTAAAGATTTTAAATTTAGATTATGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTgag                                                                  <  1:6085913/148‑1 (MQ=255)

                                                                               tctcTGACGGTTTTAAATTTGGATTTTGATAAACCGAGGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AATAAACCTAATAATGTCGATAGGATTATACTTAAACTTGCTACTGACAGGTGCCGCCTTCCAGTAAATGCAATTTgaga                                                                 <  1:3548213/148‑1 (MQ=255)

                                                                                 tctAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAAt                                                               <  1:10487254/148‑1 (MQ=255)

                                                                                  ctAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATg                                                              <  1:190107/148‑1 (MQ=255)

                                                                                  ctAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATg                                                              >  1:3748201/1‑148 (MQ=255)

                                                                                    aaaGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGtt                                                            <  1:6860344/148‑1 (MQ=255)

                                                                                     aaGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGttt                                                           <  1:5503438/148‑1 (MQ=255)

                                                                                     aaGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGttt                                                           <  1:1995754/148‑1 (MQ=255)

                                                                                      aGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑ACAAAACCTAATTATATCGAGACGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTa                                                          >  1:2684627/1‑148 (MQ=255)

                                                                                      aGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTa                                                          >  1:704046/1‑148 (MQ=255)

                                                                                      aGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTa                                                          >  1:4198810/1‑148 (MQ=255)

                                                                                       gATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAg                                                         >  1:5827011/1‑148 (MQ=255)

                                                                                       gATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAg                                                         >  1:3961127/1‑148 (MQ=255)

                                                                                        aTTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGc                                                        >  1:11477869/1‑148 (MQ=255)

                                                                                          tttAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCaa                                                      <  1:9956251/148‑1 (MQ=255)

                                                                                          tttAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCaa                                                      <  1:6193340/148‑1 (MQ=255)

                                                                                          tttAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCaa                                                      >  1:2549002/1‑148 (MQ=255)

                                                                                             aaaTTTAGATTTTGATAAATCACAGCTTTTGTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATa                                                   >  1:2414683/1‑148 (MQ=255)

                                                                                               aTTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATaaa                                                 >  1:8628735/1‑148 (MQ=255)

                                                                                                tttAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATaaaa                                                >  1:8575761/1‑148 (MQ=255)

                                                                                                 ttAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATaaaaa                                               >  1:10965311/1‑148 (MQ=255)

                                                                                                  tAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATaaaaaa                                              <  1:5361410/148‑1 (MQ=255)

                                                                                                       tttGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAAc                                         <  1:9722568/148‑1 (MQ=255)

                                                                                                       tttGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAAc                                         <  1:9526229/148‑1 (MQ=255)

                                                                                                         tGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCa                                       >  1:9338635/1‑148 (MQ=255)

                                                                                                          gATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCAt                                      >  1:1631598/1‑148 (MQ=255)

                                                                                                          gATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCAt                                      <  1:10288163/148‑1 (MQ=255)

                                                                                                             aaaTCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGc                                   >  1:1491449/1‑148 (MQ=255)

                                                                                                               aTCACAGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCaa                                 <  1:8471251/148‑1 (MQ=255)

                                                                                                                  acaGCTTTTCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTa                              >  1:4843942/1‑148 (MQ=255)

                                                                                                                        tttCTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCgg                        >  1:4320275/1‑148 (MQ=255)

                                                                                                                           cTTTAAGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATa                     >  1:2265130/1‑148 (MQ=255)

                                                                                                                               aaGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAg                 <  1:2486179/148‑1 (MQ=255)

                                                                                                                               aaGTAAATCCATAACAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAg                 >  1:3844256/1‑148 (MQ=255)

                                                                                                                               aaGTAAATCCATAAAAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAg                 >  1:5616433/1‑148 (MQ=255)

                                                                                                                                aGTAAATCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGa                >  1:7972549/1‑148 (MQ=255)

                                                                                                                                     aTCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAgg           <  1:10148040/148‑1 (MQ=255)

                                                                                                                                      tCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAggg          >  1:1110991/1‑148 (MQ=255)

                                                                                                                                      tCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAggg          <  1:5744867/148‑1 (MQ=255)

                                                                                                                                      tCCATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAggg          <  1:3673078/148‑1 (MQ=255)

                                                                                                                                       ccATAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAGGGa         <  1:1741649/148‑1 (MQ=255)

                                                                                                                                         aTAAGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCCACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAGGGACg       <  1:2721803/148‑1 (MQ=255)

                                                                                                                                           aaGAAACC‑AAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAGGGACGAt     <  1:3193648/148‑1 (MQ=255)

AGTTTTACCGATAATATACCAGAAAATAATCTGAACGGCAAATGCAATACTGGTGTAGGCTACTGATAATGTTAAGCTTTCTCTAAAGATTTTAAATTTAGATTTTGATAAATCACAGCTTTTCTTTAAGTAAATCCATAAGAAACCAAAAAAACCTAATAATGTCGAGAGGATTATACTTAAACTTGCTACTGACAGGTGCCGACTTCCAGTAAATGCAATTTGAGAATGTTTAGCAAATAAAAAAATAACCATGGCAACTATGCCGGATAAAAGACTAGGGACGATAAA  >  NC_009004/636439‑636729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`