BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_009004	1,210,283	(A)_5→6	coding (515/525 nt)	LLMG_RS13870 ←	LacI family DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_009004	1,210,278	1	.	A	98.1%	2218.5 / 22.7	577	coding (520/525 nt)	LLMG_RS13870	LacI family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (6/5); new base A (268/298); total (274/303)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 626 total aligned reads displayed.

TCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑A‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCA  >  NC_009004/1210131‑1210420

   gTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aa                                                                                                                                              <  1:11229956/148‑1 (MQ=255)

    tCAATATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGACCCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaa                                                                                                                                             <  1:11510567/148‑1 (MQ=255)

     cAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaa‑a                                                                                                                                           <  1:1609918/148‑1 (MQ=255)

     cAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaa‑a                                                                                                                                           >  1:8839436/1‑148 (MQ=255)

       aaaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaa‑aat                                                                                                                                         <  1:8573573/148‑3 (MQ=255)

        aaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTACAG‑a‑aaa‑aata                                                                                                                                        >  1:7371707/1‑145 (MQ=255)

         aTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaa‑aatat                                                                                                                                       <  1:2217251/148‑5 (MQ=255)

         aTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGATGGTGACTTTTTAGAG‑a‑aaa‑aatat                                                                                                                                       <  1:3920144/148‑5 (MQ=255)

          tttCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaa‑aatatt                                                                                                                                      <  1:2306279/148‑6 (MQ=255)

           ttCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATAttt                                                                                                                                      <  1:9572948/148‑1 (MQ=255)

           ttCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATAttt                                                                                                                                      >  1:5603627/1‑148 (MQ=255)

           ttCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTACTGGACTAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCATTGGTCTCTTTTTAGAGAA‑AAA‑ATAttt                                                                                                                                      >  1:5168593/1‑148 (MQ=255)

              tcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCt                                                                                                                                   >  1:5809761/1‑148 (MQ=255)

                ttaaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGa                                                                                                                                 <  1:11128071/146‑1 (MQ=255)

                gAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGa                                                                                                                                 <  1:2915715/148‑1 (MQ=255)

                 aaaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGAc                                                                                                                                <  1:10555993/148‑1 (MQ=255)

                 aaaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGAc                                                                                                                                <  1:8012335/148‑1 (MQ=255)

                   aaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAg                                                                                                                              >  1:10434949/1‑148 (MQ=255)

                   aaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAg                                                                                                                              <  1:10007824/148‑1 (MQ=255)

                       aTCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGa                                                                                                                          >  1:1493445/1‑148 (MQ=255)

                           gTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTa                                                                                                                      >  1:9219609/1‑148 (MQ=255)

                              ctctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAAt                                                                                                                   >  1:3323400/1‑148 (MQ=255)

                               tctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATc                                                                                                                  <  1:7427640/148‑1 (MQ=255)

                               tctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATc                                                                                                                  <  1:1235690/148‑1 (MQ=255)

                                     gAGTCATCCATGCATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTcc                                                                                                            <  1:5709435/148‑1 (MQ=255)

                                      aGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACTAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACCTCCa                                                                                                           >  1:3855925/1‑148 (MQ=255)

                                          aTCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑ATAAA‑ATATTTGCTGTCAGATGAGTTAAATCTCGTCCAAGTa                                                                                                       >  1:4787954/1‑148 (MQ=255)

                                              aTGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATATGCTGACAGATGAGTTAAATCACGTCCAAGTAGAAt                                                                                                   <  1:8158446/148‑1 (MQ=255)

                                                gTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATaa                                                                                                 <  1:11571653/148‑1 (MQ=255)

                                                gTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATaa                                                                                                 >  1:8783087/1‑148 (MQ=255)

                                                   ttGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGGTGAGTTAAATCACGTCCAAGTAGAATAAggg                                                                                              >  1:3125435/1‑148 (MQ=255)

                                                   ttGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAggg                                                                                              >  1:10154052/1‑148 (MQ=255)

                                                      gACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGaa                                                                                           <  1:7494882/148‑1 (MQ=255)

                                                       aCGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAt                                                                                          >  1:713246/1‑148 (MQ=255)

                                                        cGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAtt                                                                                         <  1:2055331/148‑1 (MQ=255)

                                                        cGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAtt                                                                                         >  1:53099/1‑148 (MQ=255)

                                                         gAACCACCAATGAAAGCTAATTTCTGATCGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAttt                                                                                        <  1:6017160/148‑1 (MQ=255)

                                                          aaCCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTc                                                                                       <  1:9470890/148‑1 (MQ=255)

                                                             catcaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCttt                                                                                    <  1:72029/145‑1 (MQ=255)

                                                               ccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTgg                                                                                  <  1:7647408/148‑1 (MQ=255)

                                                                  aTGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTa                                                                               <  1:6432754/148‑1 (MQ=255)

                                                                        gCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGc                                                                         >  1:5041216/1‑148 (MQ=255)

                                                                          tAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTa                                                                       >  1:10993791/1‑148 (MQ=255)

                                                                              ttCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAagag                                                                   >  1:5876577/1‑148 (MQ=255)

                                                                                  gATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGagcagc                                                               <  1:6975976/148‑1 (MQ=255)

                                                                                  gATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGagcagc                                                               >  1:3510136/1‑148 (MQ=255)

                                                                                   aTAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCt                                                              >  1:934269/1‑148 (MQ=255)

                                                                                     aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTg                                                            >  1:10823517/1‑148 (MQ=255)

                                                                                       ccTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGt                                                          >  1:4054106/1‑148 (MQ=255)

                                                                                        cTTTTTGAATCATGTGGGAAACAAGTAGTTGAGAGGGAGTGCGCTGGACTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGGTGAGGTAAATCACGTCCAAGTAGAAAAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTa                                                         >  1:9828453/1‑148 (MQ=255)

                                                                                        cTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGGTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGCCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTa                                                         >  1:5424856/1‑148 (MQ=255)

                                                                                         tttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAg                                                        >  1:10686460/1‑148 (MQ=255)

                                                                                         tttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAg                                                        <  1:7843361/148‑1 (MQ=255)

                                                                                          ttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGt                                                       <  1:2833191/148‑1 (MQ=255)

                                                                                            ttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCa                                                     >  1:6896795/1‑148 (MQ=255)

                                                                                            ttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCa                                                     <  1:3019676/148‑1 (MQ=255)

                                                                                            ttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCa                                                     <  1:8473705/148‑1 (MQ=255)

                                                                                             tGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAg                                                    <  1:4730144/148‑1 (MQ=255)

                                                                                             tGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAg                                                    >  1:181175/1‑148 (MQ=255)

                                                                                              gAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAgg                                                   >  1:6583513/1‑148 (MQ=255)

                                                                                               aaTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAgaa                                                  >  1:9693189/1‑146 (MQ=255)

                                                                                               aaTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGa                                                  <  1:10293991/148‑1 (MQ=255)

                                                                                               aaTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGa                                                  >  1:2727474/1‑148 (MQ=255)

                                                                                                  cATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTa                                               <  1:320675/148‑1 (MQ=255)

                                                                                                  cATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTa                                               <  1:810792/148‑1 (MQ=255)

                                                                                                   aTGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTaa                                              >  1:4596319/1‑148 (MQ=255)

                                                                                                    tgtgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGACACTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGGCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAat                                             <  1:6053555/148‑1 (MQ=255)

                                                                                                       gagaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGGTGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATc                                          >  1:731996/1‑148 (MQ=255)

                                                                                                        agaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCa                                         <  1:706566/148‑1 (MQ=255)

                                                                                                          aaaCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGt                                       <  1:10603505/148‑1 (MQ=255)

                                                                                                          aaaCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGt                                       >  1:4350877/1‑148 (MQ=255)

                                                                                                           aaCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGtt                                      <  1:7770769/148‑1 (MQ=255)

                                                                                                            aCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGttt                                     >  1:6227197/1‑148 (MQ=255)

                                                                                                             cAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTc                                    >  1:6330226/1‑148 (MQ=255)

                                                                                                             cAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTc                                    <  1:8957839/148‑1 (MQ=255)

                                                                                                             cAAGTAGTTGAGTGGGAGTGCGTTGGTCACTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCCCGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGACAGGATTAATATCAGTTTc                                    <  1:758527/148‑1 (MQ=255)

                                                                                                                    ttGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaat                             >  1:2132658/1‑148 (MQ=255)

                                                                                                                    ttGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaat                             <  1:2487001/148‑1 (MQ=255)

                                                                                                                     tGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaata                            <  1:8242550/148‑1 (MQ=255)

                                                                                                                         tGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcc                        >  1:7972350/1‑148 (MQ=255)

                                                                                                                            gAGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGCCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctcc                     <  1:2617564/148‑1 (MQ=255)

                                                                                                                             aGTGCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATcctcct                    >  1:9075913/1‑148 (MQ=255)

                                                                                                                                gCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTaaa                 >  1:2406279/1‑148 (MQ=255)

                                                                                                                                gCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTaaa                 <  1:4912688/148‑1 (MQ=255)

                                                                                                                                gCGTTGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTaaa                 <  1:888646/148‑1 (MQ=255)

                                                                                                                                   ttGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAAtt              >  1:6078666/1‑148 (MQ=255)

                                                                                                                                    tgatctcTTTTTAAAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGTGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTa             >  1:4855886/4‑148 (MQ=255)

                                                                                                                                    tGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTa             <  1:4504276/148‑1 (MQ=255)

                                                                                                                                    tGGTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTa             <  1:11304089/148‑1 (MQ=255)

                                                                                                                                     ggTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaa            <  1:4202655/148‑1 (MQ=255)

                                                                                                                                      gTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaaa           <  1:6539631/148‑1 (MQ=255)

                                                                                                                                      gTCTCTTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaaa           >  1:4652616/1‑148 (MQ=255)

                                                                                                                                        ctctTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAAc         >  1:3699936/1‑148 (MQ=255)

                                                                                                                                        ctctTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAAc         <  1:1898790/148‑1 (MQ=255)

                                                                                                                                          ctTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTg       <  1:8321893/148‑1 (MQ=255)

                                                                                                                                          ctTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTg       <  1:6769864/148‑1 (MQ=255)

                                                                                                                                          ctTTTTAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTg       >  1:1372771/1‑148 (MQ=255)

                                                                                                                                           tttttAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGc      >  1:8673753/1‑148 (MQ=255)

                                                                                                                                               tAGAGAA‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCa  >  1:3400554/1‑148 (MQ=255)

                                                                                                                                               tAGAG‑A‑AAATATATTTGCTGACAGATGTGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATCGCTATGGGCAGATTGGTAGTCAGGGTTAATATGAGTTTCATCTAATAATCCTCCTAAAATTAAAAATGCATCa  >  1:5352072/1‑148 (MQ=255)

TCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑A‑AAA‑ATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCA  >  NC_009004/1210131‑1210420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`