Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,214,915 C→T A387V (GCG→GTG)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,214,9150CT99.2% 1632.6 / ‑1.0 402A387V (GCG→GTG) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (174/224);  total (176/226)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 406 total aligned reads displayed.

AGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCC  >  CP009273/4214769‑4215060
                                                                                                                                                  |                                                                                                                                                 
aGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgtgcg                                                                                                                                                <  1:4966127/150‑1 (MQ=255)
    tGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCaa                                                                                                                                            <  1:2007480/150‑1 (MQ=255)
    tGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCaa                                                                                                                                            >  1:247532/1‑150 (MQ=255)
    tGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCaa                                                                                                                                            >  2:4122709/1‑150 (MQ=255)
     gCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAAc                                                                                                                                           <  2:6022361/150‑1 (MQ=255)
       cGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAg                                                                                                                                         <  2:4625741/150‑1 (MQ=255)
         tttGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGt                                                                                                                                       <  2:5508943/150‑1 (MQ=255)
          ttGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGATCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTg                                                                                                                                      >  2:3842891/1‑150 (MQ=255)
           tGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTgg                                                                                                                                     <  2:2694965/150‑1 (MQ=255)
               cGGCCTGGAGCCGCTGAACATTGGCGAAGATCGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAGAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGaaaa                                                                                                                                 <  2:3363863/150‑1 (MQ=255)
               cGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGaaaa                                                                                                                                 <  1:3849568/150‑1 (MQ=255)
                ggCCTGGAGCCGCTGAACATGGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAAc                                                                                                                                <  2:3629970/150‑1 (MQ=255)
                   cTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCaa                                                                                                                                            >  2:2023425/1‑135 (MQ=255)
                    tGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGTTCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgcg                                                                                                                            >  1:4455591/1‑150 (MQ=255)
                    tGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgcg                                                                                                                            >  1:4693445/1‑150 (MQ=255)
                     ggAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgcgc                                                                                                                           >  2:5362118/1‑150 (MQ=255)
                        gCCGCTGAACATTGGCGCAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCa                                                                                                                        <  2:5638197/150‑1 (MQ=255)
                        gCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCa                                                                                                                        >  1:5095637/1‑150 (MQ=255)
                        gCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCa                                                                                                                        >  2:555917/1‑150 (MQ=255)
                           gCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAATGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGAt                                                                                                                     >  1:4910447/1‑150 (MQ=255)
                              gAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTAt                                                                                                                  >  1:4994831/1‑150 (MQ=255)
                               aaCATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATc                                                                                                                 <  1:1778922/150‑1 (MQ=255)
                                 cATTGGCGAAGATAGCCTGTTTGTGAACTTGGGTTAACGCACAAAAGTCAACGTTTCCTCTAAGTTCAAGCGACTGATAAAAGAATAGAAATACAGCGAGGCGCTGGATGTCGTGCTTCAACAGGTGTAAAACGGCGCGCAGATTATCgt                                                                                                               >  2:1372667/1‑149 (MQ=255)
                                   ttGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGATGGATGACGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGata                                                                                                             >  2:351908/1‑150 (MQ=255)
                                     ggCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATc                                                                                                           <  1:1281521/150‑1 (MQ=255)
                                        gAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAAc                                                                                                        >  1:3355901/1‑150 (MQ=255)
                                          aGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACAt                                                                                                      <  1:5650576/150‑1 (MQ=255)
                                           gATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATg                                                                                                     >  1:2981400/1‑150 (MQ=255)
                                           gATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGATAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCTTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATg                                                                                                     <  2:6206305/150‑1 (MQ=255)
                                            aTAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATgg                                                                                                    >  1:6383877/1‑150 (MQ=255)
                                               gCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATg                                                                                                 <  1:393993/150‑1 (MQ=255)
                                                 cTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGaa                                                                                               <  1:3690347/150‑1 (MQ=255)
                                                 cTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGaa                                                                                               <  1:3301381/150‑1 (MQ=255)
                                                 cTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGaa                                                                                               <  2:2389020/150‑1 (MQ=255)
                                                  tGTTTGAGACCGTGGGTGAACGCACCAACGTCACCTGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAGATCAACATGGATGAAg                                                                                              <  2:4887558/150‑1 (MQ=255)
                                                   gTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAgg                                                                                             <  1:343458/150‑1 (MQ=255)
                                                    tttGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAggg                                                                                            <  2:6551857/150‑1 (MQ=255)
                                                     ttGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAATTTCAAGCGACTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAATACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGa                                                                                           <  2:1349828/150‑1 (MQ=255)
                                                        tgAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGc                                                                                        <  2:1550319/150‑1 (MQ=255)
                                                            cGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGTTCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGCGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGa                                                                                    <  2:2476224/150‑1 (MQ=255)
                                                            cGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGa                                                                                    <  2:6248566/150‑1 (MQ=255)
                                                             gTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGTTGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGAt                                                                                   >  2:2928944/1‑150 (MQ=255)
                                                             gTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGAt                                                                                   <  1:158480/150‑1 (MQ=255)
                                                                ggTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGcc                                                                                <  2:6752228/150‑1 (MQ=255)
                                                                  tGAACGCACCAACGTCACCGGGTCCGCTACGTTCAAGCGCCTGATCAAAGACGAGAAATACAGCGCGGCGCGGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTCTCGATATCAACATGGATGAAGGGATGCTCGATGCCGa                                                                              <  2:1789582/150‑1 (MQ=255)
                                                                   gAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGaa                                                                             >  2:5035234/1‑150 (MQ=255)
                                                                    aaCGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCCGATTATCGATATCAACAGGGATGAAGGGATGCTCGATGCCGAAg                                                                            >  2:5881651/1‑150 (MQ=255)
                                                                    aaCGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAg                                                                            <  1:5808443/150‑1 (MQ=255)
                                                                     aCGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAgc                                                                           <  1:6783345/150‑1 (MQ=255)
                                                                          ccAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGa                                                                      >  2:5623816/1‑150 (MQ=255)
                                                                           cAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGAt                                                                     >  1:6589591/1‑150 (MQ=255)
                                                                           cAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGAt                                                                     >  2:6052411/1‑150 (MQ=255)
                                                                           cAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGAt                                                                     <  2:1688618/150‑1 (MQ=255)
                                                                             aCGTCACCGGTTCCGCTAAGTTCTAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATgg                                                                   <  2:3331098/150‑1 (MQ=255)
                                                                               gTCACCGGTACCGCACAGTTCAAGCGCCTGAACAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCACCAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTg                                                                 <  1:6805823/150‑1 (MQ=255)
                                                                                tCACCTGTTCCGCTAAGTTCAAGCGCCTGATAAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGAGCGTAAACAGGTGGAAAACGGCGCGCAGATTATCGATATAAACATGGATGAAGGGATGCTCGATGCCGACGCGGCGATGGTGc                                                                >  2:123815/1‑150 (MQ=255)
                                                                                 cACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCg                                                               >  2:1838379/1‑150 (MQ=255)
                                                                                 cACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCg                                                               >  1:2136880/1‑150 (MQ=255)
                                                                                 cACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCg                                                               >  2:290714/1‑150 (MQ=255)
                                                                                          cGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCaa                                                      >  1:4535336/1‑150 (MQ=255)
                                                                                           gCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAAt                                                     >  1:2707681/1‑150 (MQ=255)
                                                                                             tAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCt                                                   >  2:782342/1‑150 (MQ=255)
                                                                                             tAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCt                                                   <  1:5201245/150‑1 (MQ=255)
                                                                                              aaGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTg                                                  <  2:3456726/150‑1 (MQ=255)
                                                                                               aGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGa                                                 <  1:3760786/150‑1 (MQ=255)
                                                                                                gTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGAGCGTTTTCTCAATCTGAt                                                >  1:5303278/1‑150 (MQ=255)
                                                                                                 ttCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGAtt                                               <  1:4810225/150‑1 (MQ=255)
                                                                                                   cAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGc                                             >  2:4441501/1‑150 (MQ=255)
                                                                                                     aGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCg                                           >  1:878148/1‑150 (MQ=255)
                                                                                                       cgcCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGt                                         <  1:837647/150‑1 (MQ=255)
                                                                                                        gcCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTg                                        <  1:4252895/150‑1 (MQ=255)
                                                                                                         ccTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGTTGGAAAACGGCGCGAAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGa                                       >  2:4156753/1‑150 (MQ=255)
                                                                                                            gATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCACCATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAAcc                                    <  2:3032833/150‑1 (MQ=255)
                                                                                                            gATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAAcc                                    <  2:1961179/150‑1 (MQ=255)
                                                                                                            gATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAAcc                                    >  1:110470/1‑150 (MQ=255)
                                                                                                            gATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAAcc                                    >  1:6532060/1‑150 (MQ=255)
                                                                                                              tCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCgg                                  >  1:4373355/1‑150 (MQ=255)
                                                                                                               cAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGa                                 <  2:2539913/150‑1 (MQ=255)
                                                                                                                aaaGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGTat                                <  2:4846606/150‑1 (MQ=255)
                                                                                                                  agaagaGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGatat                              >  1:4597813/1‑150 (MQ=255)
                                                                                                                    cagagAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACCTGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATAtcg                            <  2:4027716/149‑1 (MQ=255)
                                                                                                                         aaaTACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATAtcgctcgc                       <  2:6384717/150‑1 (MQ=255)
                                                                                                                          aaTACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTcgcg                      <  2:4938999/150‑1 (MQ=255)
                                                                                                                           aTACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGt                     >  2:2276168/1‑150 (MQ=255)
                                                                                                                            tACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCTATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTg                    >  1:6055177/1‑150 (MQ=255)
                                                                                                                              cAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGcc                  <  2:5753549/150‑1 (MQ=255)
                                                                                                                                  gAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGAtt              >  1:2038833/1‑150 (MQ=255)
                                                                                                                                  gAGGCGCTGGATGTCGTGCGACAACAGGTGGAAAACGGCGCGCTGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGTTGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCACGCGTGCCGAtt              <  1:2579155/150‑1 (MQ=255)
                                                                                                                                   aGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTa             >  2:3968368/1‑150 (MQ=255)
                                                                                                                                   aGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTa             <  1:317214/150‑1 (MQ=255)
                                                                                                                                   aGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTa             >  1:4120691/1‑150 (MQ=255)
                                                                                                                                   aGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTa             >  1:776248/1‑150 (MQ=255)
                                                                                                                                   aGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTa             <  2:4282357/150‑1 (MQ=255)
                                                                                                                                       gcTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGAt         <  1:5873297/150‑1 (MQ=255)
                                                                                                                                       gcTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGAt         >  1:6112382/1‑150 (MQ=255)
                                                                                                                                        cTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATc        >  2:1038148/1‑150 (MQ=255)
                                                                                                                                           gATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGAc     >  1:1392884/1‑150 (MQ=255)
                                                                                                                                             tGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGActc   <  2:3268324/150‑1 (MQ=255)
                                                                                                                                             tGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGActc   >  1:1637212/1‑150 (MQ=255)
                                                                                                                                              gTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGActcc  >  1:4335354/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCC  >  CP009273/4214769‑4215060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑