BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,493,435	(T)_6→7	intergenic (‑187/‑440)	insG ← / → yjhB	IS4 transposase/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,493,429	1	.	T	98.2%	1470.6 / 18.7	389	intergenic (‑181/‑446)	insG/yjhB	IS4 transposase/putative transporter
Reads supporting (aligned to +/- strand): ref base . (5/2); new base T (194/184); total (199/190)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 428 total aligned reads displayed.

GATTCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑T‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGAC  >  CP009273/4493280‑4493574

  ttCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑t‑t                                                                                                                                                    >  1:1797906/1‑150 (MQ=255)

   tCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑t‑t‑t                                                                                                                                                  <  2:736484/150‑1 (MQ=255)

   tCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑t‑t‑t                                                                                                                                                  <  1:1988247/150‑1 (MQ=255)

        cacaGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑t‑t‑t‑t‑tttg                                                                                                                                           >  2:4020375/1‑148 (MQ=255)

         acaGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCACTTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGAT‑T‑T‑T‑TTGa                                                                                                                                           <  1:465345/150‑1 (MQ=255)

         acaGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑t‑t‑t‑t‑tttga                                                                                                                                          <  1:346399/150‑4 (MQ=255)

                cTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACCTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGta                                                                                                                                    >  2:4899101/1‑150 (MQ=255)

                cTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGta                                                                                                                                    <  2:5161107/150‑1 (MQ=255)

                 tGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGtat                                                                                                                                   >  1:1607127/1‑150 (MQ=255)

                    aTCGGTCCACTGATCCTAAACGGCTCGGCATTAATCCCGGTTCAGGTGTTTGTAACAACCGATCAGCTACAAAAATATGCACACTTTGTGACATCAGTATCAAAACGCCTTTTGGTATGTGGATTGCGTGTT‑T‑T‑T‑TTGATCTGGTATAAc                                                                                                                                <  2:6075752/150‑1 (MQ=255)

                    aTCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAAc                                                                                                                                >  1:3945638/1‑150 (MQ=255)

                    aTCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAAc                                                                                                                                <  1:6336541/150‑1 (MQ=255)

                     tCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACa                                                                                                                               <  2:3192554/150‑1 (MQ=255)

                     tCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACa                                                                                                                               >  1:5517254/1‑150 (MQ=255)

                      cGGTCAACTGATACTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAg                                                                                                                              <  2:4445558/150‑1 (MQ=255)

                       ggTCACCTGATCCGAAACTGATCGGCCTTACTCTAGGTTCTGGAGTTTGTAACAAACTCTCAGCTACAAAAAGAGGCTCACTTGGTGACATCAGTAACACAACGCGTTTTGTTATGTGGCTTGCTTG‑TAT‑T‑T‑TTGATCTGGTATAACAgg                                                                                                                             <  2:6311437/150‑1 (MQ=255)

                       ggTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAgg                                                                                                                             <  1:4952027/150‑1 (MQ=255)

                         tCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGta                                                                                                                           <  2:1824487/150‑1 (MQ=255)

                           aaCTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGtata                                                                                                                         <  1:2356846/150‑1 (MQ=255)

                              tGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAg                                                                                                                      >  1:43914/1‑150 (MQ=255)

                              tGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAAATTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAg                                                                                                                      >  2:2671546/1‑150 (MQ=255)

                               gATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAgg                                                                                                                     >  2:1024771/1‑150 (MQ=255)

                                 tCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGta                                                                                                                   <  1:1199961/150‑1 (MQ=255)

                                      aaCTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAg                                                                                                              >  1:5627566/1‑150 (MQ=255)

                                       aCTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGa                                                                                                             <  1:6968705/150‑1 (MQ=255)

                                       aCTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGa                                                                                                             <  2:4279706/150‑1 (MQ=255)

                                          gATCGGCATTAATCTTGGTTCTGGTGATTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATACAGGTATACAGaaaa                                                                                                          <  1:864041/150‑1 (MQ=255)

                                           aTCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAg                                                                                                         <  2:5418097/150‑1 (MQ=255)

                                            tCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGc                                                                                                        <  2:6616506/150‑1 (MQ=255)

                                               gCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCaaa                                                                                                     <  1:5770624/150‑1 (MQ=255)

                                                cATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAg                                                                                                    >  2:1651687/1‑150 (MQ=255)

                                                cATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTAAAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAg                                                                                                    <  2:1942322/150‑1 (MQ=255)

                                                 cctAATCTTGGTTCTGGTGTTTGAAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGa                                                                                                   <  2:7002668/148‑1 (MQ=255)

                                                  ttAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAATaa                                                                                                  >  2:1566376/1‑150 (MQ=255)

                                                  ttAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaa                                                                                                  <  2:6698073/150‑1 (MQ=255)

                                                  ttAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaa                                                                                                  >  1:6832988/1‑150 (MQ=255)

                                                  ttAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaa                                                                                                  <  2:2218418/150‑1 (MQ=255)

                                                   tAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATTTGCTCTATTTGTGACATCAGTAACAAAACGCGTTTTTTTATGAGGATTGCTTG‑T‑TAT‑T‑TTGATCTGGTATCACATGTATCACGGTATACAGAAAAGCAAAGaaa                                                                                                 <  1:1714174/150‑1 (MQ=255)

                                                    aaTCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAAt                                                                                                <  2:2151483/150‑1 (MQ=255)

                                                     aTCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATa                                                                                               <  1:5950496/150‑1 (MQ=255)

                                                     aTCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTGTAGTTATGTGGATTGCTTG‑T‑TGT‑T‑TTGATCTGGTATAACAGGTATCAAGGTATACAGAACAGCAAAGAAATa                                                                                               >  2:6440267/1‑150 (MQ=255)

                                                      tCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATAc                                                                                              <  1:6008628/150‑1 (MQ=255)

                                                        ttGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAATGGTATACAGAAAAGCAAAGAAATACTg                                                                                            >  2:2057403/1‑150 (MQ=255)

                                                         tGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGc                                                                                           <  2:5228127/150‑1 (MQ=255)

                                                            ttCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaaa                                                                                        >  2:4779149/1‑150 (MQ=255)

                                                             tCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAg                                                                                       <  2:2487036/150‑1 (MQ=255)

                                                              cTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAgg                                                                                      <  1:529293/150‑1 (MQ=255)

                                                              cTGGTGATTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTAAAAAGGTATACAGAAAAGCAAAGAAAGACTGCAAAgg                                                                                      <  2:2861158/150‑1 (MQ=255)

                                                                ggTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                                                    <  1:4298733/150‑1 (MQ=255)

                                                                ggTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                                                    <  2:4975035/150‑1 (MQ=255)

                                                                ggTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                                                    <  1:639549/150‑1 (MQ=255)

                                                                   gtTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAAc                                                                                 <  1:3104372/150‑1 (MQ=255)

                                                                      tGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATATAGGTATACAGAAAAGCAATGAAATACTGCAAAGGAAGACAGc                                                                              >  2:4175416/1‑150 (MQ=255)

                                                                      tGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc                                                                              >  2:2953329/1‑150 (MQ=255)

                                                                         aaCAAACTCTCAGCTACAAAAATATGCTCATGTTGTGACATCAGTAACAAAACGCGGTTTGGATAGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATCAAGGTAGACAGAATGGGGAAGAAATACTGCAGAGGAAAACAGCtag                                                                           >  2:2399690/1‑149 (MQ=255)

                                                                         aaCAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCtat                                                                           >  1:4089936/1‑150 (MQ=255)

                                                                         aaCAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCtat                                                                           >  1:3017443/1‑150 (MQ=255)

                                                                            acaCTAACAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAAc                                                                        <  1:5046182/148‑1 (MQ=255)

                                                                             aaCTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACg                                                                       <  1:5534248/150‑1 (MQ=255)

                                                                             aaCTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACg                                                                       >  1:6592647/1‑150 (MQ=255)

                                                                               cTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAATCAGCTATAACGTa                                                                     >  2:3360026/1‑150 (MQ=255)

                                                                               cTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTa                                                                     <  2:5973018/150‑1 (MQ=255)

                                                                                tagcAGCTACAAACAAATCCTCAATTTCTGACATCACTAACAAAACGCTTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGAACTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTaa                                                                    <  2:2565765/147‑1 (MQ=255)

                                                                                tATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGCTCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTaa                                                                    <  2:3117820/150‑1 (MQ=255)

                                                                                   cAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCt                                                                 <  1:2570596/150‑1 (MQ=255)

                                                                                    aGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTa                                                                <  1:4789060/150‑1 (MQ=255)

                                                                                       tACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTaaat                                                             >  2:3690707/1‑149 (MQ=255)

                                                                                       tACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑T‑T‑T‑TGTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAATGGAAAACAGCTATAACGTAAGCtcacg                                                             >  1:6443085/1‑146 (MQ=255)

                                                                                          aaaaaTATGCACAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaa                                                          <  2:2284579/150‑1 (MQ=255)

                                                                                           aaaaTCTGCTCAATTTGTGACATCAGTAACAGGACGCGTTTTGTTATGTGGATTGCTTG‑T‑T‑TGT‑TTGTTCTGGTATATCAGGTCTACAGGTATTGAGAAAAGCAAAGTACTACTGCAAAGGAAATCAGCTAGAACGGATGCTCACGtatt                                                         >  2:5584359/1‑148 (MQ=255)

                                                                                           aaaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAATCTAAAGtaat                                                         >  2:2994810/1‑150 (MQ=255)

                                                                                           aaaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaat                                                         >  1:2515213/1‑150 (MQ=255)

                                                                                           aaaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaat                                                         >  1:3485005/1‑150 (MQ=255)

                                                                                           aaaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaat                                                         <  1:4219033/150‑1 (MQ=255)

                                                                                             aaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaataa                                                       >  1:3265102/1‑150 (MQ=255)

                                                                                              atatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAAc                                                      >  1:1046843/1‑150 (MQ=255)

                                                                                              atatGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAAc                                                      <  1:6786023/150‑1 (MQ=255)

                                                                                                 tGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAAGAACctc                                                   <  2:4566205/150‑1 (MQ=255)

                                                                                                  gCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACctct                                                  <  2:5540580/150‑1 (MQ=255)

                                                                                                   cTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCtctc                                                 <  1:7123906/150‑1 (MQ=255)

                                                                                                     cAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAg                                               >  1:971679/1‑150 (MQ=255)

                                                                                                      aaTTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGt                                              >  1:3770732/1‑150 (MQ=255)

                                                                                                        tttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCt                                            >  1:1857147/1‑150 (MQ=255)

                                                                                                        tttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCt                                            >  2:1549661/1‑150 (MQ=255)

                                                                                                        tttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCt                                            >  2:6123475/1‑150 (MQ=255)

                                                                                                         ttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCtt                                           >  1:3724736/1‑150 (MQ=255)

                                                                                                              acaacaGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGGATACAGAAAAGCACAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTc                                      <  1:4493612/150‑1 (MQ=255)

                                                                                                               cATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCa                                     >  1:6101480/1‑150 (MQ=255)

                                                                                                                 tCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCagt                                   >  2:4051576/1‑148 (MQ=255)

                                                                                                                     tAACAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGAc                               <  2:5866402/150‑1 (MQ=255)

                                                                                                                        cAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGaa                            >  1:2188383/1‑150 (MQ=255)

                                                                                                                        cAAAACGCGTTTTGTTATGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGaa                            <  1:204917/150‑1 (MQ=255)

                                                                                                                           aactctttttGTTATGTGTATTGCTTGTT‑T‑T‑T‑TTGATATGTTATAACAGGTATAAAGGTATACATAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAggg                         <  2:1231441/144‑1 (MQ=255)

                                                                                                                                        aTGTGGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAAc            <  2:1076580/150‑1 (MQ=255)

                                                                                                                                         tgtgGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATACAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATACAAcc           <  1:5373824/150‑1 (MQ=255)

                                                                                                                                         tgtgGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAAcc           >  2:1465508/1‑150 (MQ=255)

                                                                                                                                         tgtgGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAAcc           <  2:6828170/150‑1 (MQ=255)

                                                                                                                                         tgtgGATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAAcc           >  1:6672160/1‑150 (MQ=255)

                                                                                                                                             gATTGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGaa       <  1:3364643/150‑1 (MQ=255)

                                                                                                                                               ttGCTTGTT‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACg     <  1:1504949/150‑1 (MQ=255)

GATTCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTG‑T‑T‑T‑T‑TTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGAC  >  CP009273/4493280‑4493574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`