Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,613,656 C→T R94C (CGC→TGC)  marR → transcriptional repressor of multiple antibiotic resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,613,6560CT99.2% 1618.3 / NA 396R94C (CGC→TGC) marRtranscriptional repressor of multiple antibiotic resistance
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (156/237);  minor base A (0/3);  total (156/240)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 398 total aligned reads displayed.

TGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTC  >  CP009273/1613510‑1613802
                                                                                                                                                  |                                                                                                                                                  
  ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                                                                                                               <  2:5697717/150‑1 (MQ=255)
  ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                                                                                                               <  2:1235192/150‑1 (MQ=255)
  ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                                                                                                               <  2:4391671/150‑1 (MQ=255)
  ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                                                                                                               <  1:4493537/150‑1 (MQ=255)
      gctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTAc                                                                                                                                           >  1:6500654/1‑150 (MQ=255)
        tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                                                                                                         >  1:4994960/1‑150 (MQ=255)
        tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                                                                                                         >  1:2227723/1‑150 (MQ=255)
         ctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTgg                                                                                                                                        <  2:3413215/150‑1 (MQ=255)
         ctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTgg                                                                                                                                        <  2:6547277/150‑1 (MQ=255)
         ctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTgg                                                                                                                                        <  1:5465614/150‑1 (MQ=255)
            tCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTaa                                                                                                                                     <  2:7146877/150‑1 (MQ=255)
             ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTaaa                                                                                                                                    >  1:3582424/1‑150 (MQ=255)
               gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCTGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAAc                                                                                                                                  <  2:2947258/150‑1 (MQ=255)
               gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAAc                                                                                                                                  >  1:5138042/1‑150 (MQ=255)
               gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAAc                                                                                                                                  <  1:6362029/150‑1 (MQ=255)
               gCTGCGCGGCGTGTATGACTCCGGTTGAACTGAAAAAGGTAGTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAAc                                                                                                                                  <  2:5077409/150‑1 (MQ=255)
                cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACt                                                                                                                                 >  2:1538906/1‑150 (MQ=255)
                     cggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTacca                                                                                                                            <  2:4152361/150‑1 (MQ=255)
                     cggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTacca                                                                                                                            <  2:1038827/150‑1 (MQ=255)
                         gtgtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcgg                                                                                                                        <  1:1053479/150‑1 (MQ=255)
                          tgtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcggc                                                                                                                       <  1:3449871/150‑1 (MQ=255)
                           gtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcggcg                                                                                                                      <  2:2841178/150‑1 (MQ=255)
                            tATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcggcgg                                                                                                                     >  1:1382920/1‑150 (MQ=255)
                                aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcg                                                                                                                 >  1:161696/1‑150 (MQ=255)
                                 cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcgg                                                                                                                >  2:6864471/1‑150 (MQ=255)
                                  tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                                                                                                               <  1:6632947/150‑1 (MQ=255)
                                  tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTAGGCTGGATCGCCGGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACACGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                                                                                                               <  2:2811773/150‑1 (MQ=255)
                                    cGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCaa                                                                                                             >  2:3699569/1‑150 (MQ=255)
                                    cGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCaa                                                                                                             >  1:684398/1‑150 (MQ=255)
                                     ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAat                                                                                                            >  2:6429204/1‑150 (MQ=255)
                                       ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGATCCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAatat                                                                                                          <  2:529520/150‑1 (MQ=255)
                                        tGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtg                                                                                                         >  1:103772/1‑150 (MQ=255)
                                           aCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGa                                                                                                      <  2:591639/150‑1 (MQ=255)
                                                    ggTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCa                                                                                             >  1:2810884/1‑150 (MQ=255)
                                                      tATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATc                                                                                           <  2:6366715/150‑1 (MQ=255)
                                                          gtcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAtt                                                                                       <  2:4920252/150‑1 (MQ=255)
                                                             ggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt                                                                                    <  2:3276753/150‑1 (MQ=255)
                                                             ggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt                                                                                    >  2:4708075/1‑150 (MQ=255)
                                                              gtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGtt                                                                                   <  2:5475527/150‑1 (MQ=255)
                                                               tcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTg                                                                                  <  1:3723437/150‑1 (MQ=255)
                                                                cgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTgg                                                                                 <  1:1813057/150‑1 (MQ=255)
                                                                  aCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc                                                                               >  2:3517439/1‑150 (MQ=255)
                                                                  aCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc                                                                               <  2:1923322/150‑1 (MQ=255)
                                                                     tGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAgg                                                                            <  2:5754282/150‑1 (MQ=255)
                                                                        gAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGAcc                                                                         >  1:4757810/1‑150 (MQ=255)
                                                                        gAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGAcc                                                                         >  2:548561/1‑150 (MQ=255)
                                                                           cACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGc                                                                      <  1:7183010/150‑1 (MQ=255)
                                                                                    gTATGCTGGATCGCCTGGTCTGTAATGGCTGGGTGGTAAGGTTGCCGTTCCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAAt                                                             <  1:3394601/150‑1 (MQ=255)
                                                                                    gTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAAt                                                             <  1:1183901/150‑1 (MQ=255)
                                                                                      aTGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTa                                                           <  2:1677754/150‑1 (MQ=255)
                                                                                       tGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTaa                                                          <  2:517928/150‑1 (MQ=255)
                                                                                         cTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACa                                                        <  1:261675/150‑1 (MQ=255)
                                                                                          tGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaa                                                       >  1:2517344/1‑150 (MQ=255)
                                                                                           ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaa                                                      <  1:914145/150‑1 (MQ=255)
                                                                                           ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaa                                                      >  2:6686795/1‑150 (MQ=255)
                                                                                             aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaa                                                    <  2:2317033/150‑1 (MQ=255)
                                                                                             aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaa                                                    <  2:2512644/150‑1 (MQ=255)
                                                                                              tCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaaa                                                   >  2:3553579/1‑150 (MQ=255)
                                                                                                 ccTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCt                                                <  1:2882384/150‑1 (MQ=255)
                                                                                                   tGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGa                                              >  2:1508115/1‑150 (MQ=255)
                                                                                                   tGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGa                                              <  1:3496709/150‑1 (MQ=255)
                                                                                                      tCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcgg                                           <  1:6136705/150‑1 (MQ=255)
                                                                                                         gTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg                                        >  2:5385436/1‑150 (MQ=255)
                                                                                                            aaGGCTGGGTGGAAAGGTTGCCGAACCCTAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg                                     <  1:6468848/150‑1 (MQ=255)
                                                                                                             aGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGa                                    >  1:960162/1‑150 (MQ=255)
                                                                                                                cTGGGTGTAAAGGTTGACGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACAGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGt                                 <  2:3030012/150‑1 (MQ=255)
                                                                                                                cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGt                                 <  2:7104980/150‑1 (MQ=255)
                                                                                                                cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGt                                 >  1:1239539/1‑150 (MQ=255)
                                                                                                                  gggTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGAACTGGTAAAACTAACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAgagc                               >  2:4331678/1‑147 (MQ=255)
                                                                                                                     tGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCaa                            <  2:820413/150‑1 (MQ=255)
                                                                                                                      ggAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACCCTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac                           <  2:891932/150‑1 (MQ=255)
                                                                                                                       gAAAGGTTGCCGAACCCGAATGACACGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAaca                          <  2:4172662/150‑1 (MQ=255)
                                                                                                                       gAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAaca                          >  1:6727813/1‑150 (MQ=255)
                                                                                                                        aaaGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAacac                         <  1:1557693/150‑1 (MQ=255)
                                                                                                                           ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTg                      <  1:5931869/150‑1 (MQ=255)
                                                                                                                           ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTg                      >  2:2846251/1‑150 (MQ=255)
                                                                                                                           ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTg                      <  1:3081528/150‑1 (MQ=255)
                                                                                                                              tGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGt                   <  2:2479784/150‑1 (MQ=255)
                                                                                                                               gCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTa                  <  2:1434168/150‑1 (MQ=255)
                                                                                                                                 cGAACCCGAATGACAAGTGCGGCGTACTGGTAAATCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                <  2:6052457/150‑1 (MQ=255)
                                                                                                                                 cGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                <  1:5362282/150‑1 (MQ=255)
                                                                                                                                 cGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                <  2:3315620/150‑1 (MQ=255)
                                                                                                                                    aCCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGc             <  1:2288604/150‑1 (MQ=255)
                                                                                                                                    aCCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATAAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGc             >  2:1322673/1‑150 (MQ=255)
                                                                                                                                       cGAATGACAAGTGCGGCGTACTGGAAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTa          >  2:3998734/1‑150 (MQ=255)
                                                                                                                                        gAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCTCCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa         >  2:1793430/1‑150 (MQ=255)
                                                                                                                                        gAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCAACAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa         <  2:3158772/150‑1 (MQ=255)
                                                                                                                                         aaggACAAGAGCGGCGTACTGGTAAAACTTACCACCGGCTGCGCGGCAATATGTGAACAATGCCATCAAGTAGTTGGCCAGGACCTGCACCAAGACTTAACACAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATGTGCTTacg        <  2:2117912/147‑3 (MQ=255)
                                                                                                                                         aaTGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAg        >  1:3348921/1‑150 (MQ=255)
                                                                                                                                         aaTGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAg        >  2:3610202/1‑150 (MQ=255)
                                                                                                                                          aTGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGa       >  2:5872484/1‑150 (MQ=255)
                                                                                                                                           tGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTgaagaa      >  1:267187/1‑150 (MQ=255)
                                                                                                                                           tGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa      <  1:2044608/150‑1 (MQ=255)
                                                                                                                                           tGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa      <  1:1711656/150‑1 (MQ=255)
                                                                                                                                           tGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa      >  1:420041/1‑150 (MQ=255)
                                                                                                                                            gACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaaa     >  1:3835049/1‑150 (MQ=255)
                                                                                                                                            gACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaaa     <  2:2014814/150‑1 (MQ=255)
                                                                                                                                             aCAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAg    <  1:1341854/150‑1 (MQ=255)
                                                                                                                                              cAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGt   <  1:325784/150‑1 (MQ=255)
                                                                                                                                               aaGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTc  >  1:1072745/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTC  >  CP009273/1613510‑1613802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑