Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,214,915 C→T A387V (GCG→GTG)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,214,9150CT99.8% 1838.5 / NA 450A387V (GCG→GTG) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (206/243);  minor base G (1/0);  total (207/243)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 452 total aligned reads displayed.

TAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCT  >  CP009273/4214768‑4215061
                                                                                                                                                   |                                                                                                                                                  
tAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgtgc                                                                                                                                                  >  1:483125/1‑150 (MQ=255)
   ccTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGATTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTc                                                                                                                                               <  2:666198/150‑1 (MQ=255)
      gCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAAc                                                                                                                                            <  2:1729876/150‑1 (MQ=255)
         gTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAgg                                                                                                                                         <  1:349646/150‑1 (MQ=255)
          tttGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGGGACCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGGTCAAGCGCCTGATCCAGGAAGAGAAATACCGCGAGGCGCTGGCTGTCGTGCGTCAACAggg                                                                                                                                        >  2:248765/1‑149 (MQ=255)
                   ccTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACgg                                                                                                                               <  1:1016984/150‑1 (MQ=255)
                    cTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgc                                                                                                                              >  2:3126946/1‑150 (MQ=255)
                     tGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgcg                                                                                                                             >  1:3283619/1‑150 (MQ=255)
                      ggAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgcgc                                                                                                                            <  2:1453634/150‑1 (MQ=255)
                       gAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGgcgcg                                                                                                                           >  2:2704265/1‑150 (MQ=255)
                           cGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGa                                                                                                                       >  2:2868542/1‑150 (MQ=255)
                            gCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGAt                                                                                                                      >  2:4981871/1‑150 (MQ=255)
                            gCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGAt                                                                                                                      <  1:5407256/150‑1 (MQ=255)
                            gCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGAt                                                                                                                      >  1:4959898/1‑150 (MQ=255)
                                aaCATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATc                                                                                                                  <  2:5107684/150‑1 (MQ=255)
                                  cATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGa                                                                                                                >  2:5381511/1‑150 (MQ=255)
                                   aTTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat                                                                                                               >  1:2605162/1‑150 (MQ=255)
                                      ggCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATc                                                                                                            <  2:5155524/150‑1 (MQ=255)
                                       gCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCa                                                                                                           >  2:1869338/1‑150 (MQ=255)
                                       gCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCa                                                                                                           <  1:3203064/150‑1 (MQ=255)
                                        cGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCaa                                                                                                          >  1:4160138/1‑150 (MQ=255)
                                         gAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCaaa                                                                                                         >  2:5790367/1‑149 (MQ=255)
                                          aaGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAATCGGCGCGCAGATTATCGATATCAACa                                                                                                        >  1:7082413/1‑150 (MQ=255)
                                          aaGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACa                                                                                                        <  1:956626/150‑1 (MQ=255)
                                           aGATAGCCTGTTTGTGATCGTGGGAGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGTAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACAt                                                                                                       <  2:879397/150‑1 (MQ=255)
                                            gATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATg                                                                                                      <  1:3754813/150‑1 (MQ=255)
                                               aGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGAt                                                                                                   >  2:1372057/1‑150 (MQ=255)
                                               aGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGGTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGAt                                                                                                   <  1:3048246/150‑1 (MQ=255)
                                                  cTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGaa                                                                                                <  1:544946/150‑1 (MQ=255)
                                                    gTTTGTGACCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAgg                                                                                              <  2:4949546/150‑1 (MQ=255)
                                                     gttGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAggg                                                                                             >  2:3107598/2‑150 (MQ=255)
                                                      ttGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTAGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGa                                                                                            <  2:2981833/150‑1 (MQ=255)
                                                       tgtgAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGAt                                                                                           <  2:2135141/150‑1 (MQ=255)
                                                       tgtgAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGAt                                                                                           <  1:170810/150‑1 (MQ=255)
                                                        gtgAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATg                                                                                          >  1:2774799/1‑150 (MQ=255)
                                                           aaCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTc                                                                                       <  1:5852683/150‑1 (MQ=255)
                                                           aaCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTc                                                                                       <  1:439841/150‑1 (MQ=255)
                                                            aCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCg                                                                                      >  2:641654/1‑150 (MQ=255)
                                                            aCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCg                                                                                      >  2:2565493/1‑150 (MQ=255)
                                                            aCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCg                                                                                      <  1:2461120/150‑1 (MQ=255)
                                                            aCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCg                                                                                      >  1:4736628/1‑150 (MQ=255)
                                                              gTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGAt                                                                                    >  1:5638524/1‑150 (MQ=255)
                                                               tGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATg                                                                                   >  2:1139443/1‑150 (MQ=255)
                                                               tGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATg                                                                                   <  2:1165849/150‑1 (MQ=255)
                                                                   tGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGa                                                                               <  2:4655718/150‑1 (MQ=255)
                                                                     aaCGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAg                                                                             <  2:452453/150‑1 (MQ=255)
                                                                        gCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAgcgg                                                                          <  2:1064424/150‑1 (MQ=255)
                                                                         cACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAgcggc                                                                         >  1:5796860/1‑150 (MQ=255)
                                                                         cACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAgcggc                                                                         <  2:4212440/150‑1 (MQ=255)
                                                                          aCCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAgcggcg                                                                        >  1:6822884/1‑150 (MQ=255)
                                                                             aaCGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATg                                                                     >  1:4219215/1‑150 (MQ=255)
                                                                              aCGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATgg                                                                    >  2:4777374/1‑150 (MQ=255)
                                                                              aCGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATgg                                                                    <  1:1258341/150‑1 (MQ=255)
                                                                                   aCCGGTTCCGCTAAGTTCAAGCGCCTGATCAACGAAGAGATATCCAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATCTCAACATGGATGAAGGGATGCTCGATGCCGAAGCTGCGATGGTGCGt                                                               <  2:3265767/150‑1 (MQ=255)
                                                                                       gTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTtc                                                           <  1:6147454/150‑1 (MQ=255)
                                                                                        ttCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTtct                                                          >  2:1331472/1‑150 (MQ=255)
                                                                                         tCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACCTGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTtctc                                                         <  1:5188277/150‑1 (MQ=255)
                                                                                           cGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCaa                                                       <  2:3917243/150‑1 (MQ=255)
                                                                                             cTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGAGGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATc                                                     >  1:6703290/1‑150 (MQ=255)
                                                                                                aGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGa                                                  <  1:767112/150‑1 (MQ=255)
                                                                                                 gTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGAt                                                 <  2:7172899/150‑1 (MQ=255)
                                                                                                 gTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGAt                                                 <  1:1991362/150‑1 (MQ=255)
                                                                                                  ttCAAGCGCCTGATCAAAGAAGAGACATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCAGAAGCGGCGATGGTGCGTTTTCTCAATCTGAtt                                                <  2:1660522/150‑1 (MQ=255)
                                                                                                  ttCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAATGGATGCTAGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGAtt                                                >  2:428424/1‑150 (MQ=255)
                                                                                                   tCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGCCGCGCAGATTATCGATATCAACATGGAGGAAGGGAAGCACGATGCCGAAGCGGCGCGGCTGCGTTTTCTCAATCTGATTg                                               >  2:4689446/1‑150 (MQ=255)
                                                                                                     aaGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTATCAATCTGATTGcc                                             <  1:2221285/150‑1 (MQ=255)
                                                                                                       gcgcCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCgg                                           <  1:1383547/150‑1 (MQ=255)
                                                                                                       gcgcCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCgg                                           <  2:3657660/150‑1 (MQ=255)
                                                                                                       gcgcCTGAACAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCgg                                           <  2:6507704/150‑1 (MQ=255)
                                                                                                            tGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAAc                                      >  2:6782017/1‑150 (MQ=255)
                                                                                                             gATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAAcc                                     <  2:5502532/150‑1 (MQ=255)
                                                                                                                cAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGa                                  >  2:3314489/1‑150 (MQ=255)
                                                                                                                 aaaGAAGAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGat                                 >  2:2297668/1‑150 (MQ=255)
                                                                                                                  cagaagaGAAAGACAGCGAGGCGCTGGATGTCGTGCGTCAACCGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGata                                <  2:6187471/149‑1 (MQ=255)
                                                                                                                  aagaagAGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGata                                >  1:3364814/1‑150 (MQ=255)
                                                                                                                   agaagaGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGatat                               <  2:4085869/150‑1 (MQ=255)
                                                                                                                   agaagaGAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGatat                               >  1:2631123/1‑150 (MQ=255)
                                                                                                                    gaagcGAAATACCGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATAtc                              <  2:6982647/150‑1 (MQ=255)
                                                                                                                      agagAAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATAtcgc                            <  1:6182972/150‑1 (MQ=255)
                                                                                                                       gagaAATACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATAtcgct                           <  1:3902676/150‑1 (MQ=255)
                                                                                                                          aaaTACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATAtcgctcgc                        >  1:4641883/1‑150 (MQ=255)
                                                                                                                            aTACAGCGAGGCGCTGGATGTCGTGCGTCACCAGGTGGAAAACGGCGCGCAGATTATCGAGATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGt                      <  2:1516661/150‑1 (MQ=255)
                                                                                                                            aTACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAACACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGt                      >  1:528414/1‑150 (MQ=255)
                                                                                                                            aTACAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGt                      >  1:1847888/1‑150 (MQ=255)
                                                                                                                               cAGCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGcc                   <  1:4031177/150‑1 (MQ=255)
                                                                                                                                 gCGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGa                 >  1:4276844/1‑150 (MQ=255)
                                                                                                                                  cGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGAt                <  1:6331744/150‑1 (MQ=255)
                                                                                                                                  cGAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGAt                <  2:6820044/150‑1 (MQ=255)
                                                                                                                                   gAGGCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGAtt               >  1:6543600/1‑150 (MQ=255)
                                                                                                                                     ggCGCTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTAt             >  1:5024164/1‑150 (MQ=255)
                                                                                                                                       cgcTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGa           <  2:7633/150‑1 (MQ=255)
                                                                                                                                       cgcTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGGGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGa           <  2:5588475/150‑1 (MQ=255)
                                                                                                                                        gcTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGAt          <  1:1527753/150‑1 (MQ=255)
                                                                                                                                         cTGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATc         >  2:2738113/1‑150 (MQ=255)
                                                                                                                                          tGGATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCg        >  1:1583088/1‑150 (MQ=255)
                                                                                                                                           ggATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGa       <  2:3798128/150‑1 (MQ=255)
                                                                                                                                            gATGTCGTGCGTCACCAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGAc      <  2:602620/150‑1 (MQ=255)
                                                                                                                                            gATGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGAc      <  2:5945972/150‑1 (MQ=255)
                                                                                                                                              tGTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGActc    <  2:1665916/150‑1 (MQ=255)
                                                                                                                                               gTCGTGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGCTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGActcc   >  1:2077943/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCT  >  CP009273/4214768‑4215061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑