breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 280,246 T→G A445A (GCT→GCG yagF → CP4‑6 prophage; dehydratase family protein
RA 1,613,656 C→T R94C (CGC→TGC)  marR → transcriptional repressor of multiple antibiotic resistance
JC JC 1,765,315 IS5 (+) +4 bp intergenic (+87/‑10) ydiL → / → ydiM putative HTH domain DNA‑binding protein/MFS superfamily sugar transport 1 family protein
RA 2,802,100 T→C pseudogene (426/1178 nt) ygaY → pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein
RA 3,959,963 G→T G63C (GGT→TGT)  rho → transcription termination factor
RA 4,214,915 C→T A387V (GCG→GTG)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 4,493,435 (T)6→7 intergenic (‑187/‑440) insG ← / → yjhB IS4 transposase/putative transporter
JC JC 4,630,235 IS30 (+) +2 bp coding (17‑18/141 nt) yjjY → uncharacterized protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 946671 949073 2403 163 [159] [157] 161 pflB–[focA] pflB,[focA]
* * ÷ CP009273 1290823 1293641 2819 161 [159] [159] 161 adhE adhE
* * ÷ CP009273 1392966 1437163 44198 160 [158] [159] 163 fnr–ldhA fnr,ogt,abgT,abgB,abgA,abgR,mcaS,smrA,ydaM,ydaN,fnrS,dbpA,ttcA,intR,ydaQ,rcbA,ralR,recT,recE,racC,ydaE,kilR,sieB,ydaF,ydaG,racR,ydaS,ydaT,ydaU,ydaV,ydaW,rzpR,rzoR,trkG,ynaK,ydaY,tmpR,lomR,insH1,lomR,stfR,tfaR,pinR,ynaE,ttcC,uspF,ompN,micC,pfo,ydbJ,hslJ,ldhA
* * ÷ CP009273 2408168 2410429 2262 160 [159] [157] 164 pta pta
* * ÷ CP009273 4369117 4370396 1280 160 [158] [158] 163 [frdD]–[frdA] [frdD],frdC,frdB,[frdA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =290 (0.880)244 (0.740) 149/300 0.9 45.7% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =32 (0.100)201 (0.690) 130/268 1.0 85.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 41 (0.140)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326132 (0.100)252 (0.860) 137/268 0.7 87.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 41 (0.140)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1391495NA (NA)309 (0.940) 162/300 0.5 98.7% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 4 (0.010)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =14 (0.040)267 (0.820) 150/298 0.8 95.0% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region