BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,185,075	A→C	9.9%	V46G (GTT→GGT)	rpsE ←	30S ribosomal protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,185,075	0	A	C	9.9%	427.4 / 8.9	233	V46G (GTT→GGT)	rpsE	30S ribosomal protein S5
Reads supporting (aligned to +/- strand): ref base A (169/35); new base C (6/23); total (175/58)
Fisher's exact test for biased strand distribution p-value = 4.10e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ACCCGTATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTT  >  NZ_CP022686/2184939‑2185214

 cccGTATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGACCTTCACGCGCTTTTCCGTAACCAAACCCAAcg                                                                                                                                           >  2:1132070/1‑138 (MQ=255)

 cccGTATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcg                                                                                                                                           >  1:429655/1‑138 (MQ=255)

  ccGTATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTACCCATAACCAAcgc                                                                                                                                          >  1:963363/1‑138 (MQ=255)

    gTATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTACCCAAAACCAACGCGAcc                                                                                                                                      >  8:726345/1‑140 (MQ=255)

     tATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGAc                                                                                                                                       >  1:833223/1‑138 (MQ=255)

            acCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCaa                                                                                                                              >  8:538268/1‑139 (MQ=255)

            acCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCAt                                                                                                                              >  4:672261/1‑140 (MQ=255)

              cTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCg                                                                                                                            >  8:1116887/1‑140 (MQ=255)

              cTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCg                                                                                                                            >  6:60589/1‑140 (MQ=255)

              cTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCg                                                                                                                            >  4:804388/1‑140 (MQ=255)

               tttAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCCTCGc                                                                                                                           >  7:972087/1‑140 (MQ=255)

               tttAACAGGGTGTTGCAGAGTGCCGTAATTCCGCGCTACGTTAATCATATTGCTCCGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGc                                                                                                                           <  4:55554/140‑1 (MQ=255)

                ttAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCg                                                                                                                            >  2:816121/1‑138 (MQ=255)

                     cgggTGTTGCAGAGTGCCGTTATTCCGCGCTACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGTAACTTCCCGCGCTTTACCGTACCCAAACCCAACGCGACCGTTACCATCGCCAACTa                                                                                                                     <  7:839141/139‑1 (MQ=255)

                       ggTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACa                                                                                                                   >  7:31426/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCTCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  5:693804/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACTCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  3:885446/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCTACCGTTACCATCGCCAACTACAg                                                                                                                  >  5:626580/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCCTCGCCAACTACAg                                                                                                                  >  3:538580/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  7:518226/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  3:163614/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  8:52693/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTCGATCGCTGCTGGAACTTCCCGCGCTATACCGTAACCAACACCAACGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  7:623745/1‑140 (MQ=255)

                        gtgtTGCAGAGTGCCGTGATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTCGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCATCTACAg                                                                                                                  >  5:1154444/1‑140 (MQ=255)

                         gtttGCAGAGGGCGGTTATTCAGCGCGACGTTACTCATATTGCGAGGGGCTTTTTCCATCGCTTTCTGGAGGGCTGCGGGAACTTCACGGGCTTTCCCTTACCAAAAACCACCGCGACCGTTACCATCGCCAACTACAGt                                                                                                                 <  6:803891/138‑1 (MQ=255)

                          gtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACTGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGATACCATCGCCAACTACAg                                                                                                                  >  2:1223584/1‑138 (MQ=255)

                          gtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  2:1253907/1‑138 (MQ=255)

                          gtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGAGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  2:379415/1‑138 (MQ=255)

                          gtTGCAGAGAGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACAAACTCGACCGTTACCATCGCCAACTACAg                                                                                                                  >  1:725364/1‑138 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCTCTGCTGGAACTTCCCGCGCTTTACCGTAACCAAACCAAACGCGACCGTTACCATCGCCACCTACAGTCAGAGCtgtg                                                                                                       >  6:88549/1‑140 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCACAACCACCGCGACCGTTACCATCTCAAACAACAGTCAGAGcggag                                                                                                       >  7:74796/1‑136 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgtg                                                                                                       >  5:932091/1‑140 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgtg                                                                                                       >  3:1114267/1‑140 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtg                                                                                                         >  2:16336/1‑138 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGGTACCATCGCCAACTACAGTCagagaggtg                                                                                                       >  3:293870/1‑135 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACAAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgtg                                                                                                       >  6:1016469/1‑140 (MQ=255)

                                   tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTATTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGACAGAGCtgtg                                                                                                       >  3:265078/1‑140 (MQ=255)

                                    gCCGTTATTCAGCGCGACGTTAATCATATTGCCACGGGATTTTTCCATCGCTTTCTGGATCGCTGCTGTAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGa                                                                                                      <  8:1074353/140‑1 (MQ=255)

                                     ccGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGCCCGTTACCATCGCCAACTACAGTCAGCGCTGTGaa                                                                                                     >  3:85142/1‑140 (MQ=255)

                                     ccGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgtg                                                                                                       >  2:484212/1‑138 (MQ=255)

                                     ccGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCggtg                                                                                                       >  1:1030410/1‑138 (MQ=255)

                                     ccGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGaa                                                                                                     >  8:776751/1‑140 (MQ=255)

                                     ccGTTATTCAGCGCGACGTTAATCATATTGCGACGGACTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTCCCATCGCCAACTACAGTCAGAGCtgtg                                                                                                       >  1:307034/1‑138 (MQ=255)

                                      cGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACATTCAGAGCTGTGAAg                                                                                                    >  8:437795/1‑140 (MQ=255)

                                        ttATTCAGCGCGACGTTAATAATTTTGCGAGGGGCTTTTTCCATCGGTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACAAAACCAAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAg                                                                                                    <  2:32874/138‑1 (MQ=255)

                                                cgcgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATAc                                                                                          >  3:461757/1‑140 (MQ=255)

                                                cgcgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATAc                                                                                          >  5:771576/1‑140 (MQ=255)

                                                cgcgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATAGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAACCCCACGCGTCCGTTCCCTTCGCCAACTACAGTCACTGCTGTGCAGGAGCACATAc                                                                                          >  8:368189/1‑140 (MQ=255)

                                                  cgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAATGAGAAAATAc                                                                                          >  2:316707/1‑138 (MQ=255)

                                                  cgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGa                                                                                        >  3:358567/1‑140 (MQ=255)

                                                          aTCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAGCCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCttt                                                                                >  4:328575/1‑140 (MQ=255)

                                                            cATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCttt                                                                                >  2:771803/1‑138 (MQ=255)

                                                               atTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACgg                                                                           >  3:989164/1‑140 (MQ=255)

                                                               atTGCGACGGGCTTTTTCCATCGCTTTCTGGATAGCTGCTGGAACTTAAAGAGATTTACCGTACCCACAAACAACGCGACCGTTACCATCGCCCACTACACTCAGAGGTGTGATGGTGAAAATACGACCACCTGTCACgg                                                                           >  5:464777/1‑140 (MQ=255)

                                                                 tGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGCCCACCTTTAACgg                                                                           >  1:382168/1‑138 (MQ=255)

                                                                 tGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACgg                                                                           >  1:282939/1‑138 (MQ=255)

                                                                  gCGACGGGCTTTTTCCATCGCTTTGTGGATCGCTGCTGGAAATTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGttt                                                                        <  6:467109/140‑1 (MQ=255)

                                                                   cGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGCCCACCTTTAACGGtttt                                                                       >  8:156003/1‑140 (MQ=255)

                                                                     aCGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTaa                                                                              >  7:1104395/1‑131 (MQ=255)

                                                                     aCGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAg                                                                     >  4:967086/1‑140 (MQ=255)

                                                                        ggCTTTTTCCATCGCTTTCTGTATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATa                                                                  >  6:941947/1‑140 (MQ=255)

                                                                        ggCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGCCCGTGACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATa                                                                  >  5:637/1‑140 (MQ=255)

                                                                        ggCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGa                                                                    >  2:648979/1‑138 (MQ=255)

                                                                         gCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAc                                                                 >  8:678226/1‑140 (MQ=255)

                                                                         gCTTTTTCCATCGCTTTCTGGATCGCTGCGGGACCTTCCCGCGCTTTACCTTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAc                                                                 <  8:1026518/140‑1 (MQ=255)

                                                                         gCTTTTTCCATCGATTTCTGGATCGCTGCTGTAACTTCACGCGATTTACCTTAACCAAACCCACCGCGACCGTTACCATCGCCAACTCCAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAc                                                                 <  8:913566/140‑1 (MQ=255)

                                                                         gCTTTATCCATCGCTTTCTGGCTCGCTGCTGTAACTTCACGCGCTTTACCGTAACCAAACCCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAc                                                                 <  4:310100/140‑1 (MQ=255)

                                                                           tttttCCATCGCTTTCTGTCGCGCTGGTGCAAATCCACGCGCTTTACCGAAACAAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAcgc                                                               <  5:1014187/140‑1 (MQ=255)

                                                                             tttCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCATTACCGTAACCAAAACCAACGCGACCGTGACCATCGCCAACAACAGTCAGAGCTGAGAAGTAGAAAATACGACCACCTTTAACGGTTTTAGATACGCgg                                                             >  3:939708/1‑140 (MQ=255)

                                                                                 cATCGCTTCCTGGTGCGCCGCGGGACCTTCACGCGCTTACCCGAACCCACCACCAACGCGACCGTTACCCTCGCCAACTACAGTCTGAGCTGTTAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGtt                                                           <  1:1206044/138‑1 (MQ=255)

                                                                                   ccGCTTGCTGGCGGGCTGCTGGACCTTCCGGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTAcc                                                       <  6:783312/139‑1 (MQ=255)

                                                                                     gCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTAcc                                                       >  2:128194/1‑138 (MQ=255)

                                                                                     gCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGGTTAcc                                                       >  2:57429/1‑138 (MQ=255)

                                                                                                cGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCAAACTACAGTAAGAGCTGTGAAGGAGAAAATACGCCCACCTTTAAAGGTTTTAGATACGCGGTTTACCGCGATCAGCtttt                                          >  6:326078/1‑140 (MQ=255)

                                                                                                   tgctgGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTc                                         >  1:1015485/1‑138 (MQ=255)

                                                                                                             tCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGcc                             >  4:1115440/1‑140 (MQ=255)

                                                                                                             tCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGcc                             >  3:616921/1‑140 (MQ=255)

                                                                                                              cACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAATATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCa                            >  5:366790/1‑140 (MQ=255)

                                                                                                               aCGCGCTTTACCGTAACCAAAACCACCGCGCCCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGCCCACCTTGACCGGTTTTAGATACGCGGTTTCCCGCGATCAGCTTTTCCTGCAGTTCGCCAg                           >  4:848890/1‑140 (MQ=255)

                                                                                                                ggcgcTTTACCTAAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCa                            <  1:12705/137‑1 (MQ=255)

                                                                                                                cgcgCTTTACCGTAACCAAAACCAACGCGACAGTTACCATCGCCAACTACAGTCAGATCTGTGAAGGAGAAAATACGCCCACATTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGc                          >  5:1085390/1‑140 (MQ=255)

                                                                                                                      ttACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACAACAGTCAGAGCTGTGAAGGAGAAAATACGCCCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTGTCCTGCAGTTCGCCAGATTGttt                    >  4:521723/1‑140 (MQ=255)

                                                                                                                       tACCGTAACCAAAACCAACGCTACAGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACTAACACCTTTAACGCTTTTAGATACGCGTTTTACAGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGtttt                   >  5:28469/1‑140 (MQ=255)

                                                                                                                           gTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGCTCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGa               >  6:214544/1‑140 (MQ=255)

                                                                                                                            aaacaaaaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAt              <  5:172165/139‑1 (MQ=255)

                                                                                                                              accaaaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGGGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgt            >  4:42971/1‑140 (MQ=255)

                                                                                                                               ccaaaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgtg           >  4:362048/1‑140 (MQ=255)

                                                                                                                               ccaaaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtg             >  1:566980/1‑138 (MQ=255)

                                                                                                                                caaaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgt            >  1:191116/1‑138 (MQ=255)

                                                                                                                                 aaacccacCGCGACCGTTACCATCGCCAACTACAGTCAGAGCGGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAg         <  3:980200/140‑1 (MQ=255)

                                                                                                                                 aaacccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgtg           <  2:22384/138‑1 (MQ=255)

                                                                                                                                 aaaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgtg           >  1:602662/1‑138 (MQ=255)

                                                                                                                                  acacacaCGCGACCGTTACCATCGCCAACTACAGTCAGAGGTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTGACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGAGAGc        <  7:411261/134‑1 (MQ=255)

                                                                                                                                  aaaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGc        <  3:1202526/140‑1 (MQ=255)

                                                                                                                                  aaaccaaCGCGACCGTGACCATCGCGACGTACAGTAACAGCTTAGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGTGTTACCGCGATCAGCTTATCATGCAGTTCGCCAGCTTTTTTTTCGATGTGAGc        <  6:1199055/140‑1 (MQ=255)

                                                                                                                                  aaacaaacGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGGGGTTTACCGCGATCAGCTTTTCCTGCAGTGCGCCAGCTTGTTTTTCGATGTGAGc        <  6:693804/140‑1 (MQ=255)

                                                                                                                                  aaacaaacGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGa          <  1:1171155/138‑1 (MQ=255)

                                                                                                                                   aaccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGcc       >  8:60682/1‑140 (MQ=255)

                                                                                                                                    accaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCa      <  3:1030928/140‑1 (MQ=255)

                                                                                                                                     ccaaCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGcc       >  1:934161/1‑138 (MQ=255)

ACCCGTATGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTT  >  NZ_CP022686/2184939‑2185214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`