breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 47,347 T→G 12.5% V663G (GTC→GGC)  vgrG → type VI secretion system tip protein TssI/VgrG
RA NZ_CP022686 192,589 T→A 8.2% L667Q (CTG→CAG)  rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 238,285 G→C 9.6% V98V (GTC→GTG sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 8.7% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 269,582 A→C 7.7% F260C (TTT→TGT)  selO ← protein adenylyltransferase SelO
RA NZ_CP022686 340,516 A→C 13.1% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 8.3% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 359,896 T→G 8.2% E24A (GAG→GCG)  yoaG ← DUF1869 domain‑containing protein
RA NZ_CP022686 360,290 T→G 10.8% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 15.7% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 402,874 A→C 10.8% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 585,422 A→C 11.4% V585G (GTG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 596,637 T→G 10.5% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 20.9% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 19.4% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 16.0% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 14.6% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 682,630 A→C 7.1% V173G (GTT→GGT)  cobU ← bifunctional adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase
RA NZ_CP022686 682,694 G→T 6.0% R152S (CGT→AGT)  cobU ← bifunctional adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase
RA NZ_CP022686 693,001 A→C 7.0% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 14.0% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 10.6% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,757 T→G 25.4% K290N (AAA→AAC wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 19.4% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 11.2% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 838,396 G→T 8.0% E118D (GAG→GAT rcnA → nickel/cobalt efflux protein RcnA
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,029 C→A 21.1% intergenic (+98/‑159) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,046 G→T 16.2% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,051 G→T 15.3% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 19.7% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,005,085 C→A 9.3% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,397 T→A 11.8% intergenic (‑246/‑27) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,034,493 T→G 8.9% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,100,906 C→G 9.9% G200R (GGC→CGC)  aroC ← chorismate synthase
RA NZ_CP022686 1,171,408 C→G 6.9% intergenic (‑26/+14) CIW80_RS05830 ← / ← CIW80_RS05835 tRNA‑Ala/tRNA‑Ala
RA NZ_CP022686 1,249,393 C→A 9.5% intergenic (‑40/+46) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,353,233 A→C 16.1% V72G (GTT→GGT)  yfiF ← tRNA/rRNA methyltransferase
RA NZ_CP022686 1,360,709 T→G 6.6% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 13.7% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 9.6% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,600 C→A 15.7% D85Y (GAT→TAT)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,073 T→A 8.8% D29E (GAT→GAA bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,081 C→A 11.3% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,396,420 T→G 9.1% intergenic (+26/‑211) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,447,198 T→A 9.4% V35V (GTA→GTT hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,479,602 G→T 10.5% intergenic (‑11/+182) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,607 A→C 13.9% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 10.5% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,257 A→T 13.9% S232C (AGT→TGT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,298 A→T 10.8% V245V (GTA→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,328 G→T 15.9% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,359 G→T 12.1% V266L (GTG→TTG) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 18.2% V266V (GTG→GTT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,319 T→G 19.4% M20R (ATG→AGG)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,362 G→C 16.5% A34A (GCG→GCC fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,000 G→C 15.5% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 24.9% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 9.1% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,674,224 T→G 15.3% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 15.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.9% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 8.3% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,032 G→T 9.8% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,047 G→T 12.1% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,716,154 A→C 12.9% T45P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,180 A→C 11.6% K53N (AAA→AAC rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,833,749 G→T 15.1% V466V (GTC→GTA gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,768 A→C 15.2% M460R (ATG→AGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,860,077 G→T 19.4% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,911,602 T→G 11.8% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,923 T→G 20.4% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,964,332 A→C 8.3% G27G (GGT→GGG mug ← G/U mismatch‑specific DNA glycosylase
RA NZ_CP022686 2,027,390 A→C 6.5% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,047,984 A→C 6.5% R624R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 12.9% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.2% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,070,788 A→C 9.6% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,076,554 T→G 9.9% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,083,926 T→G 7.2% R269R (CGT→CGG kdsD → arabinose‑5‑phosphate isomerase KdsD
RA NZ_CP022686 2,110,457 T→G 10.7% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,461 A→T 7.7% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.8% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,472 T→G 7.8% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,150,071 T→G 9.0% G290G (GGT→GGG prmA → 50S ribosomal protein L11 methyltransferase
RA NZ_CP022686 2,159,660 G→T 7.3% D163Y (GAT→TAT)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,275,771 A→C 11.1% D47A (GAC→GCC)  feoA → ferrous iron transporter A
RA NZ_CP022686 2,316,671 A→C 5.7% G474G (GGT→GGG glgB ← 1,4‑alpha‑glucan branching enzyme
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,361,489 T→G 9.4% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,393,013 T→G 5.3% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 21.2% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,516,888 A→T 6.3% T766T (ACA→ACT upaG → trimeric autotransporter adhesin UpaG
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,743,578 T→G 13.8% noncoding (80/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,168 A→C 7.2% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,769,181 A→C 10.0% V52G (GTG→GGG)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 18.8% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,808,127 T→G 7.9% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,868,740 T→G 26.5% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,742 A→C 15.2% P21P (CCT→CCG fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 21.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 10.5% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,871,449 T→G 5.6% D140A (GAC→GCC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,856 T→G 7.8% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,428 A→C 9.6% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,933,245 A→C 10.4% S26R (AGC→CGC)  frwB → PTS fructose‑like transporter subunit IIB
RA NZ_CP022686 2,994,693 T→G 9.8% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,020,229 T→G 12.1% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,111,371 T→G 5.9% S361A (TCG→GCG)  gltP → glutamate/aspartate:proton symporter GltP
RA NZ_CP022686 3,136,935 T→G 7.6% S163R (AGC→CGC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 9.9% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,348,342 A→T 6.2% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,055 G→T 5.2% intergenic (+754/‑37) CIW80_RS16815 → / → CIW80_RS16820 helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,387,116 A→C 8.5% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.2% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,845 A→C 8.8% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,229 T→G 21.2% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 11.1% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,604,564 G→T 17.5% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,606,036 G→T 7.1% E250* (GAA→TAA)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,625,704 T→G 10.0% R157R (CGT→CGG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,634,614 A→C 6.0% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 18.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,524 T→G 6.3% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,495 T→G 7.4% G134G (GGT→GGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,256 G→T 11.6% E1583* (GAA→TAA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,859,840 G→T 5.1% I569I (ATC→ATA vat ← vacuolating autotransporter toxin Vat
RA NZ_CP022686 3,868,846 T→G 13.4% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,868,857 T→G 12.9% N379T (AAC→ACC)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,289 A→C 14.4% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 13.2% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,935,324 T→G 10.2% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,942,556 T→G 7.0% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,949,969 T→G 9.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 4,014,225 C→G 7.7% intergenic (+71/‑273) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 19.1% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,133,839 T→G 11.4% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,287,456 T→G 14.0% noncoding (47/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,368,341 T→G 11.1% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,284 A→C 14.8% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,297 T→A 11.3% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 13.5% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,571,354 C→A 14.8% E87D (GAG→GAT pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,417 T→G 11.1% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,589,392 T→G 14.5% K238N (AAA→AAC CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,632,182 T→G 11.8% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,655,834 A→C 6.8% R390R (CGT→CGG iroC ← salmochelin/enterobactin export ABC transporter IroC
RA NZ_CP022686 4,762,516 T→G 12.4% K368N (AAA→AAC mfd ← transcription‑repair coupling factor
RA NZ_CP022686 4,764,883 A→T 11.3% intergenic (‑63/‑198) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,441 T→G 9.7% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,809,462 T→G 7.3% R57R (CGT→CGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,191 T→G 15.1% L106R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,909,243 T→A 6.3% intergenic (‑9/+25) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,947,335 T→G 8.6% V42G (GTT→GGT)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA NZ_CP022686 4,951,521 A→C 8.3% T39P (ACC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,141 G→T 13.4% M155I (ATG→ATT ompG → monomeric porin OmpG
RA NZ_CP022686 5,041,191 G→A 5.3% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,181 T→A 7.6% S604R (AGT→AGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,250 A→T 7.8% V627V (GTA→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 11.2% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,137 G→T 10.1% V1256V (GTG→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,178 T→G 11.9% I1270S (ATC→AGC)  CIW80_RS25525 → EntS/YbdA MFS transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 684–2618 3043–2957 340–2360 4339 [4059] [4083] 4275 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2771–2840 5510–3244 405–2740 4462 [4238] [3744] 4346 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein