breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 299,433 C→A 6.5% intergenic (‑185/‑17) osmE ← / → nadE osmotically‑inducible lipoprotein OsmE/ammonia‑dependent NAD(+) synthetase
RA NZ_CP022686 316,794 A→C 6.4% T113P (ACG→CCG)  ynjE → thiosulfate sulfurtransferase YnjE
RA NZ_CP022686 349,106 A→C 13.7% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,930 C→G 26.6% intergenic (+114/‑734) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 14.8% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 15.1% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,391 C→A 12.4% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 15.6% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 17.8% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 13.5% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,023 G→T 12.3% intergenic (+92/‑165) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,027 A→T 11.1% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,046 G→T 8.7% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,105 A→C 13.8% intergenic (+174/‑83) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 12.0% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,401 A→C 14.2% V955G (GTG→GGG)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,040,909 A→C 13.4% E232D (GAA→GAC rbn → ribonuclease BN
JC NZ_CP022686 1,061,329 Δ6 bp 5.1% coding (42‑47/945 nt) lrhA ← transcriptional regulator LrhA
RA NZ_CP022686 1,100,906 C→G 8.7% G200R (GGC→CGC)  aroC ← chorismate synthase
RA NZ_CP022686 1,100,944 T→G 12.7% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,172,466 G→C 12.5% A130P (GCA→CCA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,213,457 G→C 14.9% L428V (CTG→GTG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,360,724 G→T 10.1% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,568 G→T 11.7% V95V (GTC→GTA rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,396,426 T→G 9.8% intergenic (+32/‑205) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,479,619 T→A 12.2% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,535,318 A→T 15.5% M20L (ATG→TTG)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,558,395 A→C 11.2% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,543 A→C 8.4% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,610,221 A→C 5.5% F272V (TTT→GTT)  lysA ← diaminopimelate decarboxylase
RA NZ_CP022686 1,632,486 C→G 7.7% A161G (GCT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,632,501 T→G 10.4% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,894 T→A 9.8% Q237L (CAG→CTG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,634,345 A→C 6.8% S87A (TCA→GCA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 13.3% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 10.7% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 9.8% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 15.8% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,215 C→A 6.5% A65E (GCA→GAA)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,770,715 C→A 5.8% intergenic (‑108/+392) iha ← / ← CIW80_RS08795 bifunctional siderophore receptor/adhesin Iha/IS3 family transposase
RA NZ_CP022686 1,833,762 A→C 15.0% L462R (CTG→CGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,786 A→C 14.3% V454G (GTG→GGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,949,883 A→C 5.1% G395G (GGA→GGC CIW80_RS09700 → RNA‑guided endonuclease TnpB family protein
RA NZ_CP022686 1,954,923 T→G 23.0% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,965,173 A→C 12.8% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 1,971,460 T→G 10.8% intergenic (+51/‑133) ebgR → / → ebgA transcriptional regulator EbgR/beta‑galactosidase subunit alpha
RA NZ_CP022686 2,047,987 A→C 7.6% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 17.0% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 11.7% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,804 A→C 9.9% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,110,457 T→G 12.0% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,472 T→G 9.3% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,136 T→G 9.3% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,050 A→C 6.4% R54R (CGT→CGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 15.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,207,100 C→A 13.4% intergenic (‑124/+45) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,215 A→C 8.0% F38V (TTC→GTC)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,277,483 G→T 5.8% G536G (GGG→GGT feoB → Fe(2+) transporter permease subunit FeoB
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 18.0% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,607 T→G 7.9% L36R (CTT→CGT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,383,165 G→T 5.1% intergenic (+56/+58) uspA → / ← CIW80_RS26230 universal stress protein UspA/hypothetical protein
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,522 T→A 12.2% V19V (GTA→GTT yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 16.8% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 30.1% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,516,712 A→T 10.6% T708S (ACG→TCG)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,558,864 T→G 9.5% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,705 A→C 12.6% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,733,575 C→G 10.0% A54G (GCC→GGC)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,578 C→A 7.1% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 15.6% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 9.4% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,128 A→C 9.3% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,769,186 C→A 13.9% M50I (ATG→ATT rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 18.1% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,780,127 C→A 9.8% P683T (CCA→ACA)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,821,644 A→C 11.1% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,868,740 T→G 17.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 13.1% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,915 T→G 6.8% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,385 C→A 6.0% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,428 A→C 9.6% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 3,098,847 A→C 7.5% intergenic (+17/+22) CIW80_RS15415 → / ← actP LrgB family protein/cation/acetate symporter ActP
RA NZ_CP022686 3,136,935 T→G 9.8% S163R (AGC→CGC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 12.4% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,348,342 A→T 10.2% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 12.0% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,625 C→A 10.9% D140Y (GAT→TAT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 16.8% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,444,739 T→G 7.8% D518A (GAC→GCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,202 T→A 15.1% intergenic (‑11/+24) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 15.0% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 25.3% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,604,564 G→T 11.1% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,634,614 A→C 8.2% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 13.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 10.0% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 21.1% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 12.0% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,850,142 A→T 8.3% intergenic (+40/‑52) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,935,324 T→G 14.7% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,949,969 T→G 12.0% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,611 A→C 10.0% E73D (GAA→GAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,408 T→G 7.1% V105G (GTT→GGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 4,014,225 C→G 10.0% intergenic (+71/‑273) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 22.5% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,067,840 A→C 6.4% K7N (AAA→AAC ybaQ → HigA family addiction module antitoxin
RA NZ_CP022686 4,290,635 T→G 7.7% T109P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,335,504 T→G 8.2% K58N (AAA→AAC ybhF ← ATP‑binding cassette domain‑containing protein
RA NZ_CP022686 4,368,341 T→G 6.8% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 7.4% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,334 A→C 19.3% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,632,182 T→G 10.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 11.0% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,642,956 A→C 8.3% T734P (ACG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,783,330 T→G 12.8% E33D (GAA→GAC mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,814,990 T→G 17.7% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,918,873 T→G 10.0% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,538 C→A 18.1% A44A (GCC→GCA topA → type I DNA topoisomerase
RA NZ_CP022686 4,952,020 T→G 11.4% V205G (GTC→GGC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,162 G→C 17.7% L162F (TTG→TTC ompG → monomeric porin OmpG
RA NZ_CP022686 5,044,641 C→T 5.4% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 720 A→C 5.2% intergenic (–/‑716)  / → ompA –/–
JC pHH‑29_RBS 1,479 Δ11 bp 5.8% coding (44‑54/63 nt) ompA →
JC pHH‑29_RBS 1,522 Δ41 bp 8.6% coding (24‑64/570 nt) Aldafermin (NGM282) →
RA pHH‑29_RBS 1,874 C→T 20.3% Q126* (CAG→TAG)  Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 382–2618 3051–2957 340–2670 5723 [5647] [5681] 5710 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2775–2840 5512–3245 406–2738 4595 [4518] [4408] 5175 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 = 3899681193 (1.160)9 (0.060) 8/214 NT 5.1% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 169 (1.160)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA