breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 162,405 T→G 10.0% F46V (TTT→GTT)  ydgH → DUF1471 family protein YdgH
RA NZ_CP022686 193,743 T→G 7.9% L309V (TTA→GTA)  rsxD → electron transport complex subunit RsxD
RA NZ_CP022686 238,317 G→T 16.8% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 340,516 A→C 13.1% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 12.2% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 350,056 A→C 13.7% K241T (AAA→ACA)  yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,227 T→G 15.7% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,256 C→A 22.4% G25V (GGA→GTA)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 364,775 C→A 10.8% E193* (GAA→TAA)  yeaY ← Slp family lipoprotein YeaY
RA NZ_CP022686 372,574 A→C 16.1% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 377,274 A→C 14.3% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 379,587 C→G 5.8% intergenic (+22/‑33) manZ → / → yobD PTS mannose transporter subunit IID/DUF986 family protein
RA NZ_CP022686 402,874 A→C 6.3% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,948 A→T 14.9% intergenic (+132/‑716) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 17.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,727 A→C 7.5% L448V (TTG→GTG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,848 A→C 14.8% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,568 G→T 8.5% intergenic (+134/‑334) CIW80_RS03550 → / → CIW80_RS03555 YaiO family outer membrane beta‑barrel protein/transposase
RA NZ_CP022686 694,391 C→A 12.6% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 11.6% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,024 C→G 8.6% R32P (CGA→CCA)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 23.7% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 18.1% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 21.3% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 13.1% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 857,274 T→G 8.2% G78G (GGT→GGG yehL → MoxR family ATPase
RA NZ_CP022686 881,733 A→C 12.1% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,739 A→C 17.3% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 896,350 G→T 11.5% E2* (GAA→TAA)  yeiG → S‑formylglutathione hydrolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,051 G→T 11.6% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 12.9% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 954,516 A→C 9.8% L41V (TTG→GTG)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 957,112 T→G 10.1% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 8.8% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,065,978 A→C 8.8% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,065,997 A→C 12.4% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,907 T→G 16.0% E199D (GAA→GAC aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 13.7% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,249,402 G→C 5.9% intergenic (‑49/+37) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,280,117 T→G 7.1% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,285,186 G→C 7.7% intergenic (‑54/+96) rlmN ← / ← ndk bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN/nucleoside‑diphosphate kinase
RA NZ_CP022686 1,360,724 G→T 11.9% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,081 C→A 23.2% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,383,405 T→G 5.8% F240V (TTT→GTT)  ypjD → inner membrane protein YpjD
RA NZ_CP022686 1,424,342 C→G 7.2% intergenic (‑38/+25) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,464,955 G→T 6.5% E158* (GAA→TAA)  pphB → protein‑serine/threonine phosphatase
RA NZ_CP022686 1,479,607 A→C 11.5% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,500,012 C→A 7.6% P257T (CCG→ACG)  ygcE → FGGY‑family carbohydrate kinase
RA NZ_CP022686 1,504,298 A→T 7.7% V245V (GTA→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,328 G→T 14.6% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,351 A→C 11.0% N263T (AAC→ACC)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,353 G→C 6.9% A264P (GCT→CCT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 8.7% A264A (GCT→GCA) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,307 A→C 9.4% D16A (GAC→GCC)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,353 G→T 28.7% M31I (ATG→ATT fucI → L‑fucose isomerase
RA NZ_CP022686 1,558,391 T→A 9.3% V463E (GTA→GAA)  vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,558,395 A→C 10.0% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,287 G→T 5.5% T96T (ACG→ACT CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,569 A→C 6.4% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,625,489 G→T 5.5% Q281K (CAA→AAA)  ygeV ← sigma‑54‑dependent Fis family transcriptional regulator
RA NZ_CP022686 1,632,480 T→G 8.2% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,632,486 C→G 6.2% A161G (GCT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,632,501 T→G 11.9% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,901 T→G 8.2% T235P (ACG→CCG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 15.0% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 13.1% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 9.0% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.0% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,975 A→T 8.8% intergenic (‑585/‑70) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,023 T→A 8.4% intergenic (‑633/‑22) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,047 G→T 10.6% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,833,745 T→G 19.4% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,843,277 A→C 7.1% F339V (TTT→GTT)  glcA ← glycolate permease GlcA
RA NZ_CP022686 1,857,686 T→G 5.9% intergenic (+34/+1) CIW80_RS09240 → / ← lptG aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme/LPS export ABC transporter permease LptG
RA NZ_CP022686 1,860,077 G→T 14.9% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,911,602 T→G 9.1% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 8.5% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,365 A→C 6.9% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 16.5% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,160 T→G 7.7% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,175 T→G 6.1% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,521 A→C 10.9% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,094 G→T 8.7% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.6% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,056,180 A→C 5.3% R610R (CGT→CGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,056,187 G→C 9.8% A608G (GCA→GGA)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,076,554 T→G 12.5% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,481 A→T 6.6% F4L (TTT→TTA rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,115 T→G 10.5% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 12.9% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 13.8% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,176 T→G 7.5% G146G (GGT→GGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,688 A→C 7.4% Y172S (TAC→TCC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,179,907 T→A 7.3% A25A (GCA→GCT rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,184,330 G→T 9.6% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,063 T→A 6.0% Y50F (TAC→TTC)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,215 A→C 6.1% F38V (TTC→GTC)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,226,139 A→C 6.2% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,250,454 A→C 7.8% S40A (TCG→GCG)  dam ← adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,173 T→G 7.8% L108V (TTG→GTG)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,357,324 A→C 15.4% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 14.1% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,386,496 T→G 6.8% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,013 T→G 5.2% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 13.9% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,443,289 A→C 16.1% V9V (GTT→GTG bcsQ ← cellulose biosynthesis protein BcsQ
RA NZ_CP022686 2,460,526 A→C 15.8% V18G (GTG→GGG)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,535 A→C 8.4% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,555 A→C 7.1% G8G (GGT→GGG yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 8.6% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,216 T→C 7.2% S542S (TCT→TCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,426 T→A 10.2% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 9.2% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,550,305 T→G 5.6% K151N (AAA→AAC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,624,291 G→T 8.6% S140Y (TCC→TAC)  yidK ← solute:sodium symporter family transporter
RA NZ_CP022686 2,638,855 C→A 7.8% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,743,563 T→G 12.6% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,153 A→C 7.7% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,769,166 C→A 11.1% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,186 C→A 9.1% M50I (ATG→ATT rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 15.5% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,801,817 T→G 6.6% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,131 T→G 7.6% G442G (GGT→GGG ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,808,135 C→A 12.5% P444T (CCT→ACT)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,834,886 A→C 6.8% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 24.3% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 20.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 7.2% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 6.2% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 6.5% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 5.7% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,408 C→A 5.4% Q62K (CAA→AAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,418 C→A 8.3% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,994,696 C→G 7.8% H163D (CAT→GAT)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,017,611 A→C 7.6% T836P (ACC→CCC)  metH → methionine synthase
RA NZ_CP022686 3,017,622 A→C 13.9% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 10.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,020,414 A→C 5.8% T195P (ACC→CCC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,062,555 G→T 8.6% I219I (ATC→ATA CIW80_RS15250 ← acyl CoA:acetate/3‑ketoacid CoA transferase
RA NZ_CP022686 3,066,354 T→G 5.7% K134N (AAA→AAC CIW80_RS15265 ← hypothetical protein
RA NZ_CP022686 3,136,923 A→C 12.1% S167A (TCG→GCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 15.3% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,184,651 A→C 8.9% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,210,871 G→T 8.7% intergenic (+7/‑29) CIW80_RS15980 → / → CIW80_RS15985 tRNA‑Gly/tRNA‑Gly
RA NZ_CP022686 3,265,084 T→G 11.6% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,265,096 G→T 12.0% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,278,003 G→T 18.0% E281D (GAG→GAT ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,281,004 T→G 8.2% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,052 T→G 6.2% F91V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,062 T→G 9.8% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,064 T→G 10.5% L95V (TTG→GTG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,297,333 T→G 13.9% F828V (TTT→GTT)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,342 A→T 7.0% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 6.6% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,116 A→C 11.9% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,390,629 C→A 14.8% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 9.9% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,845 A→C 8.7% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,218 G→C 13.4% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,221 T→G 10.6% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 10.9% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,224 T→G 14.3% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 18.6% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 12.0% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 7.4% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,504,054 T→G 7.3% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,508,399 A→C 6.1% D289A (GAC→GCC)  dnaK → molecular chaperone DnaK
RA NZ_CP022686 3,516,075 T→G 8.3% V213G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,526,230 G→T 6.3% A209S (GCC→TCC)  dapB → 4‑hydroxy‑tetrahydrodipicolinate reductase
RA NZ_CP022686 3,604,564 G→T 17.6% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,604,565 A→T 17.3% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 11.2% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,703,648 A→C 5.9% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,714,342 T→G 7.7% L275V (TTG→GTG)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,731,466 T→C 100% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 100% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,473 Δ1 bp 100% intergenic (+27/‑28) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 22.9% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,736,542 C→A 8.6% G70V (GGA→GTA)  mltD ← murein transglycosylase D
RA NZ_CP022686 3,748,050 T→G 6.2% K22N (AAA→AAC CIW80_RS18735 ← hypothetical protein
RA NZ_CP022686 3,770,524 T→G 6.8% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,493 G→T 13.7% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,266 A→C 7.6% Y1586S (TAC→TCC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,868,846 T→G 8.9% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,500 G→T 7.3% intergenic (‑106/+78) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,910,616 C→A 6.9% G145G (GGG→GGT yahC ← DUF1097 domain‑containing protein
RA NZ_CP022686 3,925,283 G→C 9.7% S154T (AGC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 10.6% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,935,324 T→G 9.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 9.7% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 7.9% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,977,656 A→C 8.4% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,988,213 T→G 6.4% V313G (GTT→GGT)  secD → protein translocase subunit SecD
RA NZ_CP022686 4,014,238 C→G 9.9% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 9.9% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 8.6% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 21.6% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,941 T→G 11.7% G130G (GGT→GGG adk → adenylate kinase
RA NZ_CP022686 4,056,946 T→G 7.2% V132G (GTT→GGT)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 17.7% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,133,839 T→G 10.7% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,188,926 A→C 8.4% V327G (GTC→GGC)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA NZ_CP022686 4,214,218 T→A 6.5% intergenic (‑6/+43) CIW80_RS21085 ← / ← CIW80_RS21090 tRNA‑Gln/tRNA‑Met
RA NZ_CP022686 4,269,248 A→C 6.2% intergenic (+443/‑108) sdhB → / → sucA succinate dehydrogenase iron‑sulfur subunit SdhB/2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,280,095 T→G 7.8% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,285,435 G→T 5.0% V317V (GTG→GTT tolB → Tol‑Pal system beta propeller repeat protein TolB
RA NZ_CP022686 4,331,903 T→A 11.2% Y301F (TAC→TTC)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,905 A→C 10.0% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,929 C→A 14.6% M292I (ATG→ATT ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 13.0% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 11.1% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,334 A→C 23.5% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,461,745 G→T 9.2% intergenic (+99/‑71) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,485,614 T→G 11.9% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,285 A→C 8.8% K97T (AAG→ACG)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,535,355 A→C 8.8% E120D (GAA→GAC appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,571,454 A→C 7.5% V54G (GTC→GGC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,459 G→T 9.7% F52L (TTC→TTA pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 12.5% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,605,584 C→G 12.7% R54P (CGC→CCC)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,615,707 A→T 24.0% E120V (GAA→GTA)  CIW80_RS23065 → hypothetical protein
RA NZ_CP022686 4,632,182 T→G 6.9% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,033 T→G 31.5% intergenic (+31/‑90) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,041 A→C 17.6% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 22.8% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 15.4% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,814,990 T→G 12.5% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,993 C→G 6.5% L72V (CTG→GTG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,668 T→G 18.1% V150V (GTA→GTC) ‡ minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,669 A→T 9.9% V150E (GTA→GAA) ‡ minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 15.2% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,850,805 T→A 6.6% Y208F (TAT→TTT)  ycgB ← SpoVR family protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,178 T→G 11.0% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,905,232 A→T 7.5% S120C (AGC→TGC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,909,243 T→A 8.9% intergenic (‑9/+25) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,916,128 A→C 8.2% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,980,895 A→C 10.0% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,001,141 G→T 8.0% M155I (ATG→ATT ompG → monomeric porin OmpG
RA NZ_CP022686 5,001,164 A→C 12.1% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,304 T→G 12.1% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 7.7% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,921 C→T 7.7% N1184N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 8.2% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.2% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
JC pHH‑29_RBS 1,392 Δ4 bp 8.8% intergenic (–/‑41)  / → ompA –/–
JC pHH‑29_RBS 1,534 Δ13 bp 72.3% coding (36‑48/570 nt) Aldafermin (NGM282) →
RA pHH‑29_RBS 1,700 G→T 7.1% G68* (GGA→TGA)  Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 681–2618 3048–2957 340–2368 5676 [5442] [5492] 5761 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2769–2898 5510–3245 348–2742 5488 [5253] [4514] 5567 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3899666 =281 (1.180)50 (0.240) 25/218 NT 17.7% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 221 (1.070)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681271 (1.140)41 (0.200) 26/218 NT 15.2% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 221 (1.070)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA