breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 11.0% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 238,303 C→A 13.7% M92I (ATG→ATT sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 8.3% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 340,507 G→C 7.7% L34V (CTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 360,227 T→G 11.6% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 9.7% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,940 A→T 16.0% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 585,420 A→C 13.4% W586G (TGG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 585,422 A→C 14.3% V585G (GTG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 601,295 T→G 20.8% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,757 C→A 11.0% G438W (GGG→TGG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 17.6% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,090 C→A 19.3% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 7.7% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 973,436 A→C 5.1% D253A (GAC→GCC)  rcsD → phosphotransferase RcsD
RA NZ_CP022686 1,005,085 C→A 13.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,065,997 A→C 12.0% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,197,142 T→G 11.3% G74G (GGT→GGG murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,237,918 C→A 12.9% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,249,393 C→A 9.1% intergenic (‑40/+46) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,360,724 G→T 9.8% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,081 C→A 25.5% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,086 G→T 25.8% E34* (GAA→TAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,092 T→A 17.5% Y36N (TAC→AAC)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,396,426 T→G 9.0% intergenic (+32/‑205) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,479,633 A→C 15.5% intergenic (‑42/+151) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,361 G→T 19.9% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 9.3% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,632,501 T→G 9.5% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,674,244 T→G 10.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 13.4% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,784,265 A→C 12.6% L106L (CTA→CTC CIW80_RS08870 → IS66 family transposase
RA NZ_CP022686 1,832,023 T→G 12.5% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,833,745 T→G 21.7% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,602 T→G 12.5% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,365 A→C 9.3% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,949,883 A→C 6.2% G395G (GGA→GGC CIW80_RS09700 → RNA‑guided endonuclease TnpB family protein
RA NZ_CP022686 2,016,170 A→C 5.9% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 15.4% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,554 T→G 9.2% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 15.5% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,126 A→T 8.3% S130C (AGT→TGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 8.9% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 8.8% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,075 A→C 12.8% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,340,220 A→C 7.6% F106V (TTT→GTT)  livF ← high‑affinity branched‑chain amino acid ABC transporter ATP‑binding protein LivF
RA NZ_CP022686 2,386,496 T→G 9.8% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,433,341 A→C 9.5% S1055A (TCG→GCG)  bcsC ← cellulose synthase complex outer membrane protein BcsC
RA NZ_CP022686 2,516,872 A→C 7.0% K761T (AAA→ACA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,888 A→T 5.5% T766T (ACA→ACT upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,891 T→C 7.0% A767A (GCT→GCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 12.2% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 12.9% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,550,305 T→G 11.0% K151N (AAA→AAC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,624,291 G→T 12.2% S140Y (TCC→TAC)  yidK ← solute:sodium symporter family transporter
RA NZ_CP022686 2,717,400 T→G 8.8% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,733,571 T→G 12.5% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,586 A→C 14.7% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 8.3% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,569 T→G 9.1% noncoding (71/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,834,886 A→C 9.8% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 24.2% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,449 T→G 9.1% D140A (GAC→GCC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,977,481 T→G 7.4% V558G (GTA→GGA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA NZ_CP022686 2,981,625 A→C 5.9% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 3,136,940 T→G 15.0% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,286,997 C→A 6.3% A101E (GCA→GAA)  cybC → cytochrome b562
RA NZ_CP022686 3,348,342 A→T 6.6% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 9.4% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,376,248 A→C 5.2% K152N (AAA→AAC mtnA → S‑methyl‑5‑thioribose‑1‑phosphate isomerase
RA NZ_CP022686 3,387,116 A→C 12.8% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,387,616 G→T 7.1% I231I (ATC→ATA sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,629 C→A 10.0% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.2% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,223 G→T 18.4% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 13.6% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,583,665 A→C 6.9% Q342H (CAA→CAC ilvI → acetolactate synthase 3 large subunit
RA NZ_CP022686 3,604,565 A→T 15.3% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,731,466 T→C 41.7% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 41.7% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,570 T→G 14.0% noncoding (70/77 nt) CIW80_RS18620 → tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 24.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 13.8% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,509 A→T 10.0% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,806,262 G→T 10.6% D1585Y (GAT→TAT)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,811,468 A→T 15.4% N118Y (AAT→TAT)  CIW80_RS19090 → hypothetical protein
RA NZ_CP022686 3,868,846 T→G 11.0% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,279 T→A 10.7% Y153N (TAC→AAC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 12.9% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 12.9% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 12.6% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,933,079 A→C 5.2% R411R (CGT→CGG lacY ← lactose permease
RA NZ_CP022686 3,940,707 A→C 11.3% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 12.8% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 15.3% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,032,292 A→C 14.6% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,213,260 T→G 6.1% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,280,095 T→G 9.7% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,287,532 G→T 9.8% noncoding (45/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,368,341 T→G 11.2% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,413,654 T→G 5.6% K121N (AAA→AAC ltaE ← low‑specificity L‑threonine aldolase
RA NZ_CP022686 4,440,315 A→C 10.9% Y791S (TAT→TCT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 7.6% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,571,398 C→A 6.5% D73Y (GAT→TAT)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 10.9% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 9.1% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,764,877 T→G 13.5% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 11.7% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,809,441 T→G 7.8% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,981 T→G 9.2% W68G (TGG→GGG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,687 T→G 14.0% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,210 T→G 10.2% G112G (GGT→GGG CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,905,221 T→G 14.1% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,910,213 A→C 8.2% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,952,020 T→G 8.4% V205G (GTC→GGC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 9.0% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,001,164 A→C 16.3% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,043,347 A→G 6.2% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 11.4% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,879 C→T 5.5% G1170G (GGC→GGT CIW80_RS25525 → EntS/YbdA MFS transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 350–2618 3068–2957 340–2719 5409 [5306] [5196] 5797 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2771–2890 5510–3245 356–2740 3545 [3366] [3078] 3530 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 937548 =157 (0.870)8 (0.050) 8/234 NT 5.1% noncoding (63/77 nt) CIW80_RS04765 tRNA‑Pro
?NZ_CP022686 = 949035 149 (0.880)intergenic (+566/+139) CIW80_RS04860/CIW80_RS26000 hypothetical protein/autotransporter outer membrane beta‑barrel domain‑containing protein
* ? NZ_CP022686 3743172 =226 (1.260)17 (0.100) 10/248 NT 6.5% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 263 (1.470)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase