breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
JC NZ_CP022686 235,926 Δ7 bp 9.1% coding (940‑946/1413 nt) pykF → pyruvate kinase PykF
RA NZ_CP022686 238,317 G→T 14.8% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 9.7% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 340,511 G→C 8.1% G32G (GGC→GGG ydjK ← MFS transporter
RA NZ_CP022686 340,516 A→C 9.3% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,092 A→C 11.0% N602T (AAC→ACC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,123 A→C 9.5% K612N (AAA→AAC yeaG → protein kinase YeaG
RA NZ_CP022686 372,574 A→C 16.1% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 377,274 A→C 10.3% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 6.4% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 585,422 A→C 11.7% V585G (GTG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 596,637 T→G 11.5% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 19.1% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 23.3% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 682,630 A→C 10.2% V173G (GTT→GGT)  cobU ← bifunctional adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,008 A→T 7.6% I37I (ATT→ATA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,763 A→T 14.7% Y288* (TAT→TAA wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 828,846 A→C 11.2% L189R (CTT→CGT)  gatY ← tagatose‑bisphosphate aldolase subunit GatY
RA NZ_CP022686 829,452 G→T 17.4% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.4% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 13.1% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 20.7% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,561 G→T 16.7% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,573 C→A 12.7% intergenic (‑146/+77) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 11.3% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 881,733 A→C 14.6% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,739 A→C 20.0% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 19.6% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,051 G→T 10.5% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 18.8% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 954,525 A→C 13.3% S38A (TCT→GCT)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 957,112 T→G 15.6% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,439 T→G 24.8% P942P (CCA→CCC yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,073 A→C 9.6% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 12.0% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,419 T→A 13.3% intergenic (‑268/‑5) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,040,909 A→C 6.6% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,047,906 G→T 6.8% intergenic (‑136/+28) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,100,944 T→G 18.1% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,100,959 T→G 9.6% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,120,330 A→C 5.2% V2556G (GTC→GGC)  CIW80_RS05650 ← phase‑variable autotransporter adhesin UpaE
RA NZ_CP022686 1,166,968 A→C 6.1% G119G (GGT→GGG mntH ← Nramp family divalent metal transporter
RA NZ_CP022686 1,167,039 A→C 12.7% F96V (TTC→GTC)  mntH ← Nramp family divalent metal transporter
RA NZ_CP022686 1,174,304 A→T 6.1% intergenic (+33/‑11) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,197,147 T→G 13.5% L76R (CTG→CGG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,908 T→G 21.7% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,249,393 C→A 6.8% intergenic (‑40/+46) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,285,186 G→C 8.0% intergenic (‑54/+96) rlmN ← / ← ndk bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN/nucleoside‑diphosphate kinase
RA NZ_CP022686 1,358,650 A→T 5.2% L335F (TTA→TTT pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
RA NZ_CP022686 1,360,724 G→T 12.3% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 9.9% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 9.6% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 8.0% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,277 T→G 7.5% intergenic (‑278/+164) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,396,426 T→G 11.6% intergenic (+32/‑205) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,447,198 T→A 8.7% V35V (GTA→GTT) ‡ hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,447,199 A→C 6.9% V35G (GTA→GGA) ‡ hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,479,585 T→A 9.1% K3* (AAA→TAA)  ftsB ← cell division protein FtsB
RA NZ_CP022686 1,479,602 G→C 14.5% intergenic (‑11/+182) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,607 A→C 8.7% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,339 C→A 19.5% P259Q (CCA→CAA)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,546,996 T→A 11.0% intergenic (+18/‑16) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,000 G→C 12.4% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,003 G→C 11.3% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,008 G→C 10.0% intergenic (+30/‑4) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 12.5% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,543 A→C 8.7% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,569 A→C 10.3% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,573 T→G 12.1% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,573,897 A→C 7.6% T391P (ACC→CCC)  CIW80_RS07810 → VasL domain‑containing protein
RA NZ_CP022686 1,604,862 A→C 8.8% F379V (TTC→GTC)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,625,489 G→T 9.1% Q281K (CAA→AAA)  ygeV ← sigma‑54‑dependent Fis family transcriptional regulator
RA NZ_CP022686 1,640,925 A→C 10.9% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 17.6% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 13.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 10.9% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,047 G→T 8.5% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,710,453 A→C 7.2% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,202 A→C 10.4% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 7.9% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,833,745 T→G 19.8% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,860,077 G→T 16.0% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,865,103 A→C 8.8% L491V (TTG→GTG)  pitB ← inorganic phosphate transporter PitB
RA NZ_CP022686 1,911,580 G→T 6.1% S231R (AGC→AGA CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,911,602 T→G 13.5% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 8.3% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 22.5% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,964,353 A→C 7.8% G20G (GGT→GGG mug ← G/U mismatch‑specific DNA glycosylase
RA NZ_CP022686 1,965,173 A→C 10.4% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,009,528 A→C 5.9% R211R (CGT→CGG tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase
RA NZ_CP022686 2,016,225 T→G 7.5% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,521 A→C 10.9% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 15.9% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 12.0% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,112 C→A 8.4% A248A (GCG→GCT deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,804 A→C 7.8% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 12.5% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,588 C→A 5.5% intergenic (+58/‑175) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 10.5% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,461 A→T 6.8% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,490 C→A 6.4% M1M (ATG→ATT) † rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,236 A→C 6.1% G209G (GGT→GGG tldD ← metalloprotease TldD
RA NZ_CP022686 2,123,241 A→C 9.0% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,129,986 C→A 6.4% L150F (TTG→TTT mreD ← rod shape‑determining protein MreD
RA NZ_CP022686 2,130,829 A→C 11.8% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 9.4% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 11.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 14.0% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,075 A→C 8.3% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,208,142 A→C 6.5% V569G (GTT→GGT)  chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,361,501 C→A 7.2% S192* (TCG→TAG)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,394,287 T→G 20.5% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,523 A→C 8.6% V19G (GTA→GGA)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 13.1% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 25.0% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 10.4% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 8.4% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,533,236 A→C 5.4% L301R (CTG→CGG)  tdh ← L‑threonine 3‑dehydrogenase
RA NZ_CP022686 2,546,394 A→C 13.4% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,423 A→C 13.7% V85V (GTT→GTG waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,572,721 A→C 9.7% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 13.1% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 6.3% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,632,806 G→T 8.4% T64N (ACC→AAC)  dgoT ← MFS transporter
RA NZ_CP022686 2,638,855 C→A 7.3% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,717,385 T→G 10.3% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,733,571 T→G 11.1% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,578 C→A 6.3% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 9.5% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,596 G→T 12.9% intergenic (+11/‑32) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,743,601 G→T 11.2% intergenic (+16/‑27) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,754,994 A→T 5.7% intergenic (‑7/+41) cyaY ← / ← CIW80_RS13805 iron donor protein CyaY/hypothetical protein
RA NZ_CP022686 2,758,109 A→C 10.7% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 7.8% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,162 G→T 11.0% D52Y (GAT→TAT)  yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 8.6% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,769,186 C→A 11.3% M50I (ATG→ATT rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 13.7% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,770,486 G→T 5.4% E157* (GAA→TAA)  pldA → phospholipase A
RA NZ_CP022686 2,799,492 G→T 5.9% P86T (CCC→ACC)  CIW80_RS14015 ← tripartite tricarboxylate transporter permease
RA NZ_CP022686 2,808,123 C→A 8.2% Q440K (CAG→AAG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,821,644 A→C 8.4% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 9.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,834,898 A→C 8.0% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,867,494 C→A 5.1% A138S (GCG→TCG)  fdoI ← formate dehydrogenase cytochrome b556 subunit
RA NZ_CP022686 2,868,740 T→G 25.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 11.9% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 10.2% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 7.2% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 6.7% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,919,048 A→C 5.1% L349V (TTA→GTA)  CIW80_RS14605 ← hypothetical protein
RA NZ_CP022686 2,920,411 G→T 8.3% E63* (GAA→TAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,239 T→G 6.4% T209P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,940,870 A→C 6.0% L596R (CTG→CGG)  ppc ← phosphoenolpyruvate carboxylase
RA NZ_CP022686 2,981,625 A→C 7.1% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 9.7% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,017,622 A→C 10.6% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 13.6% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,020,233 T→G 8.3% G134G (GGT→GGG yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,066,354 T→G 9.9% K134N (AAA→AAC CIW80_RS15265 ← hypothetical protein
RA NZ_CP022686 3,070,959 T→A 9.7% P44P (CCT→CCA CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,090,070 T→A 5.2% V19V (GTA→GTT yjcB ← YjcB family protein
RA NZ_CP022686 3,090,093 G→T 5.5% R12S (CGC→AGC)  yjcB ← YjcB family protein
RA NZ_CP022686 3,090,101 A→C 8.7% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,923 A→C 12.5% S167A (TCG→GCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,935 T→G 8.9% S163R (AGC→CGC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 13.1% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,137,023 A→C 7.0% G133G (GGT→GGG phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,210,871 G→T 8.4% intergenic (+7/‑29) CIW80_RS15980 → / → CIW80_RS15985 tRNA‑Gly/tRNA‑Gly
RA NZ_CP022686 3,218,260 T→G 7.9% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,252,190 T→G 11.6% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,252,198 C→A 12.2% E219* (GAA→TAA)  CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,265,084 T→G 18.0% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,277,965 A→C 7.1% T269P (ACG→CCG)  ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,280,824 T→G 6.2% F15V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,004 T→G 6.5% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,297,360 T→G 8.0% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,387,116 A→C 9.9% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 10.7% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,188 T→A 10.9% noncoding (4/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 11.5% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 16.0% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,221 T→G 14.7% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 16.3% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 26.2% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 9.4% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 8.6% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,363 A→C 11.3% noncoding (65/87 nt) CIW80_RS17345 ← tRNA‑Leu
RA NZ_CP022686 3,604,564 G→T 12.8% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,634,614 A→C 10.0% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,650,128 T→G 10.4% V25G (GTT→GGT)  CIW80_RS18240 → hypothetical protein
RA NZ_CP022686 3,652,079 T→G 6.7% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 5.8% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,657,084 T→G 5.9% Y250S (TAC→TCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 11.9% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,661,525 T→A 6.5% N7Y (AAT→TAT)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,682,223 A→T 5.9% L260F (TTA→TTT dgt → dGTPase
RA NZ_CP022686 3,731,466 T→C 100% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 100% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,473 Δ1 bp 100% intergenic (+27/‑28) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 18.7% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 12.5% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,736,531 T→G 5.0% N74H (AAC→CAC)  mltD ← murein transglycosylase D
RA NZ_CP022686 3,736,542 C→A 7.8% G70V (GGA→GTA)  mltD ← murein transglycosylase D
RA NZ_CP022686 3,770,508 T→G 9.7% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 6.0% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,526 C→G 6.2% intergenic (‑262/+201) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,798,080 T→G 5.6% R329R (CGT→CGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,868,846 T→G 14.4% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,868,857 T→G 9.9% N379T (AAC→ACC)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,885,449 G→C 6.7% V1401V (GTG→GTC fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,892,606 A→C 5.8% N80T (AAC→ACC)  ykgF → LutB/LldF family L‑lactate oxidation iron‑sulfur protein
RA NZ_CP022686 3,928,345 T→G 9.4% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.8% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 9.7% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 9.6% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 5.9% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,949,969 T→G 8.5% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,631 A→C 10.6% H80P (CAC→CCC)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,238 C→G 11.1% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 11.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,018,989 A→C 6.2% D246A (GAC→GCC)  lon → endopeptidase La
RA NZ_CP022686 4,032,241 C→A 6.8% G89G (GGC→GGA glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 22.0% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,061,214 A→C 6.6% L432R (CTG→CGG)  ybaL ← YbaL family putative K(+) efflux transporter
RA NZ_CP022686 4,214,235 C→A 5.5% intergenic (‑23/+26) CIW80_RS21085 ← / ← CIW80_RS21090 tRNA‑Gln/tRNA‑Met
RA NZ_CP022686 4,331,940 A→C 16.2% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,372 T→G 13.7% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 10.5% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,269 A→C 6.2% F246V (TTT→GTT)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,334 A→G 18.9% Q797Q (CAA→CAG ftsK → DNA translocase FtsK
RA NZ_CP022686 4,571,459 G→T 11.5% F52L (TTC→TTA pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,632,182 T→G 9.1% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,638,424 G→T 5.6% L109L (CTG→CTT CIW80_RS23235 → S/F1C fimbrial major subunit operon transcriptional regulator
RA NZ_CP022686 4,638,444 A→C 5.3% intergenic (+17/‑355) CIW80_RS23235 → / → focA S/F1C fimbrial major subunit operon transcriptional regulator/F1C fimbrial major subunit FocA
RA NZ_CP022686 4,712,985 A→C 7.7% intergenic (‑67/+67) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,764,877 T→G 16.8% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 9.6% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,814,990 T→G 12.3% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,191 T→G 11.8% L106R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,918,873 T→G 7.2% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,952,020 T→G 7.3% V205G (GTC→GGC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,976,153 G→T 6.0% E821* (GAA→TAA)  CIW80_RS25180 → efflux RND transporter permease subunit
RA NZ_CP022686 5,001,162 G→C 12.6% L162F (TTG→TTC ompG → monomeric porin OmpG
RA NZ_CP022686 5,044,014 T→A 13.1% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,359 C→G 5.9% V1330V (GTC→GTG CIW80_RS25525 → EntS/YbdA MFS transporter
JC pHH‑29_RBS 1,388 Δ12 bp 100% intergenic (–/‑37)  / → ompA –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 380–2618 3055–2957 340–2676 5354 [4893] [5042] 5193 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2772–2890 5510–3245 356–2739 4005 [3997] [3438] 4229 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3899666 =163 (0.670)139 (0.660) 49/218 NT 50.2% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 134 (0.640)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681160 (0.660)127 (0.600) 47/218 NT 48.1% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 134 (0.640)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 4514430317 (1.310)19 (0.010)
+42 bp
9/158 NT 11.5% coding (3/1053 nt) ompA porin OmpA
?pHH‑29_RBS = 1383 143 (0.020)intergenic (–/‑53) –/ompA –/–