breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 23,792 A→C 9.2% D114A (GAC→GCC)  ydcR → PLP‑dependent aminotransferase family protein
RA NZ_CP022686 37,785 T→G 7.3% K3N (AAA→AAC ansP ← L‑asparagine permease
RA NZ_CP022686 128,070 A→C 5.7% pseudogene (26/106 nt) mgtT → protein MgtT
RA NZ_CP022686 269,578 A→C 7.7% G261G (GGT→GGG selO ← protein adenylyltransferase SelO
RA NZ_CP022686 294,971 G→T 10.1% P175T (CCG→ACG)  celF ← 6‑phospho‑beta‑glucosidase
RA NZ_CP022686 311,462 G→C 10.7% E119Q (GAA→CAA) ‡ ydjY → lipoprotein YdjY
RA NZ_CP022686 311,464 A→C 10.8% E119D (GAA→GAC) ‡ ydjY → lipoprotein YdjY
RA NZ_CP022686 340,516 A→C 10.6% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,092 A→C 9.9% N602T (AAC→ACC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,106 A→C 10.0% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 361,672 T→G 17.2% intergenic (+82/+11) CIW80_RS26330 → / ← rnd isocitrate/isopropylmalate family dehydrogenase/ribonuclease D
RA NZ_CP022686 364,748 C→G 9.9% intergenic (‑183/+22) fadD ← / ← yeaY long‑chain‑fatty‑acid‑‑CoA ligase FadD/Slp family lipoprotein YeaY
RA NZ_CP022686 372,563 T→A 6.6% V271V (GTT→GTA sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 372,574 A→C 15.6% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 436,591 A→C 5.8% intergenic (‑19/+38) CIW80_RS02305 ← / ← CIW80_RS02310 phage minor tail protein L/phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 482,321 C→A 8.2% A181A (GCG→GCT flhA ← flagellar biosynthesis protein FlhA
RA NZ_CP022686 496,135 A→C 7.7% V382V (GTT→GTG otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 498,662 A→C 9.0% C183G (TGT→GGT)  araH ← arabinose ABC transporter permease AraH
RA NZ_CP022686 531,408 A→C 10.1% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,942 G→T 15.2% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 17.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.3% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 11.6% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,001 A→C 6.8% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,402 G→T 9.3% intergenic (+162/‑34) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,457 G→T 17.1% E8* (GAA→TAA)  CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 16.6% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 777,344 G→T 10.7% S223Y (TCC→TAC)  gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 18.6% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 22.0% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,561 G→T 14.6% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 9.6% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 881,739 A→C 20.9% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 18.9% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 939,755 A→C 5.6% pseudogene (6/468 nt) CIW80_RS26375 → icd‑like protein
RA NZ_CP022686 948,035 A→T 11.3% intergenic (+104/‑153) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,046 G→T 11.2% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 9.3% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 10.4% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 15.3% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,105 A→C 10.6% intergenic (+174/‑83) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 12.5% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,401 A→C 13.7% V955G (GTG→GGG)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,000,431 T→G 24.6% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,000,439 T→G 24.6% P942P (CCA→CCC yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 17.3% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,047,883 T→G 7.6% intergenic (‑113/+51) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,065,974 G→C 7.6% A38G (GCA→GGA)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,082,212 A→C 6.1% L58V (TTA→GTA)  ubiX ← flavin prenyltransferase UbiX
RA NZ_CP022686 1,088,686 A→C 6.2% V29G (GTT→GGT)  dedA ← DedA family protein
RA NZ_CP022686 1,100,944 T→G 7.6% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,100,954 C→G 11.0% D184H (GAC→CAC)  aroC ← chorismate synthase
RA NZ_CP022686 1,100,959 T→G 12.9% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,120,323 A→T 6.4% T2558T (ACT→ACA CIW80_RS05650 ← phase‑variable autotransporter adhesin UpaE
RA NZ_CP022686 1,174,272 A→C 17.1% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,195,304 T→G 6.2% N130T (AAC→ACC)  cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA NZ_CP022686 1,342,844 A→C 6.2% V18G (GTC→GGC)  rseC ← SoxR‑reducing system protein RseC
RA NZ_CP022686 1,360,709 T→G 6.9% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 7.8% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 8.4% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 17.5% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,504,250 T→G 16.8% V229V (GTT→GTG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,308 A→T 9.3% M249L (ATG→TTG) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 10.4% M249I (ATG→ATT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,324 C→A 16.0% P254H (CCT→CAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 12.5% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,318 A→T 16.4% M20L (ATG→TTG)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,366 G→C 20.6% A36P (GCC→CCC)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,000 G→C 14.3% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 17.3% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,564,569 A→C 10.8% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,979 T→G 6.2% V327G (GTC→GGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,203 A→C 10.9% N735T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,619,248 G→T 6.6% intergenic (+37/+280) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,632,501 T→G 11.1% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,640,925 A→C 14.7% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 12.5% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 10.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 13.0% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,025 C→A 14.4% intergenic (‑635/‑20) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,047 G→T 15.9% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 11.9% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,828,113 A→C 10.1% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,871,158 A→C 7.6% V117G (GTC→GGC)  hybD ← HyaD/HybD family hydrogenase maturation endopeptidase
RA NZ_CP022686 1,888,362 T→G 9.3% V241G (GTT→GGT)  yqhG → YbjP/YqhG family protein
RA NZ_CP022686 1,911,580 G→T 11.0% S231R (AGC→AGA CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,365 A→C 5.2% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 15.3% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,975,143 G→C 7.4% intergenic (+12/‑51) ebgC → / → ygjI beta‑galactosidase subunit beta/amino acid permease
RA NZ_CP022686 2,016,153 T→G 6.4% noncoding (121/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,160 T→G 5.8% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 8.4% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,035,513 T→A 6.1% Y141F (TAT→TTT)  rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA NZ_CP022686 2,047,993 A→C 7.2% G621G (GGT→GGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 11.4% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.6% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,799 G→T 7.9% E390* (GAA→TAA)  argG → argininosuccinate synthase
RA NZ_CP022686 2,110,457 T→G 13.7% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.7% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,490 C→A 7.6% M1M (ATG→ATT) † rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 5.5% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 9.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 10.4% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 13.2% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,118 C→G 12.8% A127G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,673 C→A 15.2% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 7.6% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,075 A→C 7.1% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,194,054 G→T 7.9% Q444K (CAA→AAA)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,150 A→C 5.6% R59R (CGT→CGG tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,226,139 A→C 7.0% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,173 T→G 6.2% L108V (TTG→GTG)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,357,291 A→C 10.4% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 18.2% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,493 A→T 9.4% L189F (TTA→TTT nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,394,287 T→G 16.9% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,295 G→T 8.3% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,302 A→T 10.2% F110Y (TTC→TAC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,896 A→C 15.2% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,400,826 T→G 6.9% V11G (GTC→GGC)  shuU → heme ABC transporter permease ShuU/ChuU
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,516,426 T→A 10.0% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,872 A→C 8.0% K761T (AAA→ACA) ‡ upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,873 A→C 8.0% K761N (AAA→AAC) ‡ upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,721 A→C 10.2% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 11.2% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 6.5% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,747,591 T→G 5.8% N395T (AAC→ACC)  hemY ← protoheme IX biogenesis protein HemY
RA NZ_CP022686 2,758,109 A→C 7.7% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 7.0% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 7.4% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,769,155 A→C 9.6% S61A (TCC→GCC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,780,110 T→G 6.5% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,821,653 A→C 5.4% intergenic (+109/‑269) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 8.3% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,852,911 T→G 6.6% G172G (GGT→GGG CIW80_RS14270 → aldolase
RA NZ_CP022686 2,868,740 T→G 22.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 16.7% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 6.9% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,903,361 T→G 8.6% N205T (AAC→ACC)  hslU ← HslU‑‑HslV peptidase ATPase subunit
RA NZ_CP022686 2,904,852 T→G 9.1% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 6.5% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,428 A→C 7.3% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,203 T→G 7.0% T221P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,961,318 A→C 12.0% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,989,627 T→G 9.2% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,020,188 C→A 12.9% F119L (TTC→TTA yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,020,229 T→G 12.1% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,039,810 C→A 8.5% G42G (GGG→GGT malG ← maltose ABC transporter permease MalG
RA NZ_CP022686 3,066,354 T→G 8.4% K134N (AAA→AAC CIW80_RS15265 ← hypothetical protein
RA NZ_CP022686 3,070,979 T→G 10.6% V51G (GTA→GGA)  CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,070,986 T→G 10.5% G53G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,090,101 A→C 8.0% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,107,078 T→G 6.6% L265R (CTT→CGT)  nrfD → cytochrome c nitrite reductase subunit NrfD
RA NZ_CP022686 3,131,140 G→T 5.8% F433L (TTC→TTA alsA ← D‑allose ABC transporter ATP‑binding protein AlsA
RA NZ_CP022686 3,136,940 T→G 10.9% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,151,862 T→G 6.7% F176V (TTT→GTT)  proP → glycine betaine/L‑proline transporter ProP
RA NZ_CP022686 3,189,470 A→C 6.7% V47G (GTT→GGT)  yjeH ← L‑methionine/branched‑chain amino acid transporter
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.2% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,281,030 C→A 12.2% F83L (TTC→TTA yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,052 T→G 11.3% F91V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,062 T→G 12.9% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,287,046 A→C 5.3% K117N (AAA→AAC cybC → cytochrome b562
RA NZ_CP022686 3,348,345 G→T 8.6% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 5.3% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,629 C→A 10.5% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 11.0% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,444,785 T→G 6.0% T503P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,845 A→C 8.3% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,215 A→T 15.5% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 16.9% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 11.9% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 6.2% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,493,424 G→T 6.6% G177G (GGC→GGA arcA ← two‑component system response regulator ArcA
RA NZ_CP022686 3,496,452 A→C 13.7% I273L (ATC→CTC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,504,054 T→G 7.0% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,516,093 T→G 7.3% V219G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,570,234 T→G 11.4% L217V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,570,252 T→G 11.1% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,570,303 T→G 6.4% L240V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,604,565 A→T 18.5% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,634,614 A→C 6.8% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,657,091 C→A 10.5% E248* (GAA→TAA)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,525 T→A 8.4% N7Y (AAT→TAT)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,734,367 T→G 18.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 7.1% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 7.8% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 13.5% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 11.6% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,801,675 A→C 5.2% T56P (ACG→CCG)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,885,457 A→C 9.0% N1404T (AAC→ACC)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,925,289 A→C 14.2% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 9.8% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 13.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,935,341 T→G 6.0% V699V (GTA→GTC lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 6.0% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 8.4% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,950,011 T→G 7.3% H127P (CAC→CCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,611 A→C 9.4% E73D (GAA→GAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 10.9% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,238 C→G 12.7% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 12.0% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,284 A→C 22.2% T104P (ACC→CCC)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,032,292 A→C 14.4% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 16.2% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,987 G→C 8.0% D146H (GAC→CAC)  adk → adenylate kinase
RA NZ_CP022686 4,133,839 T→G 14.0% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,133,850 T→G 12.8% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,141,326 T→G 5.8% V73G (GTC→GGC)  entF → enterobactin non‑ribosomal peptide synthetase EntF
RA NZ_CP022686 4,286,455 T→G 9.9% V38G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,290,635 T→G 5.9% T109P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,905 A→C 7.1% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,940 A→C 17.8% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 15.3% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 14.7% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 12.6% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,429 T→G 8.5% T18P (ACG→CCG)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,284 A→C 11.3% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,413,663 C→A 6.4% M118I (ATG→ATT ltaE ← low‑specificity L‑threonine aldolase
RA NZ_CP022686 4,440,297 T→A 10.2% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,324 C→A 14.0% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→G 17.5% Q797Q (CAA→CAG ftsK → DNA translocase FtsK
RA NZ_CP022686 4,445,534 T→G 9.9% intergenic (+40/‑199) serS → / → dmsA serine‑‑tRNA ligase/dimethylsulfoxide reductase subunit A
RA NZ_CP022686 4,485,614 T→G 10.8% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,571,454 A→C 7.0% V54G (GTC→GGC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 10.8% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,589,367 T→G 5.7% T247P (ACC→CCC)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,620,262 A→C 10.7% intergenic (+6/+117) CIW80_RS23110 → / ← CIW80_RS26565 Exc2 family lipoprotein/hypothetical protein
RA NZ_CP022686 4,632,182 T→G 8.8% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 10.1% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,655,834 A→C 7.2% R390R (CGT→CGG iroC ← salmochelin/enterobactin export ABC transporter IroC
RA NZ_CP022686 4,755,357 T→G 7.5% intergenic (+32/‑367) ycfP → / → ndh alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase
RA NZ_CP022686 4,764,877 T→G 13.3% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 8.4% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 10.7% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 7.1% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 13.4% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,787,799 C→A 5.4% intergenic (‑52/‑44) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,809,441 T→G 9.4% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,809,462 T→G 6.1% R57R (CGT→CGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,990 T→G 12.1% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 7.4% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,821,347 A→C 11.8% K207N (AAA→AAC CIW80_RS24320 → prophage tail fiber N‑terminal domain‑containing protein
RA NZ_CP022686 4,839,668 T→G 14.3% V150V (GTA→GTC minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 14.5% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,221 T→G 14.5% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,916,128 A→C 6.4% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,917,402 C→A 5.9% E869* (GAA→TAA)  adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,918,873 T→G 10.1% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,947,316 G→T 8.0% A36S (GCT→TCT)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA NZ_CP022686 4,947,335 T→G 8.8% V42G (GTT→GGT)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA NZ_CP022686 4,951,545 A→C 12.3% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 7.6% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 4,990,017 T→G 6.9% V358G (GTC→GGC)  ycjM → glucosylglycerate phosphorylase
RA NZ_CP022686 5,000,034 T→G 10.3% L162R (CTC→CGC)  CIW80_RS25310 → ABC transporter ATP‑binding protein
RA NZ_CP022686 5,042,385 T→A 9.3% G672G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 11.9% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 8.6% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 9.9% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
JC pHH‑29_RBS 1,408 Δ79 bp 84.0% [ompA] [ompA]
JC pHH‑29_RBS 1,522 Δ41 bp 10.3% coding (24‑64/570 nt) Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 776–2618 2982–2957 340–2207 3330 [3031] [3104] 3513 JNG15_RS00010–[mobA] JNG15_RS00010,JNG15_RS00015,[mobA]
* * ÷ pHH‑29_RBS 2769–2909 5512–3104 196–2744 10604 [9941] [8385] 10317 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein