breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 46,672 A→C 5.7% N438T (AAC→ACC)  vgrG → type VI secretion system tip protein TssI/VgrG
RA NZ_CP022686 69,199 A→C 9.0% G253G (GGT→GGG adhP ← alcohol dehydrogenase AdhP
RA NZ_CP022686 87,520 G→T 6.9% R217S (CGT→AGT)  yddA ← ABC transporter ATP‑binding protein/permease
RA NZ_CP022686 235,846 A→C 9.1% N287T (AAC→ACC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 238,317 G→T 16.1% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 11.8% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 8.1% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 340,516 A→C 7.2% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 10.2% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 16.0% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,269 T→G 6.7% T21P (ACT→CCT)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 18.3% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 418,374 T→G 11.0% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,942 G→T 18.1% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 598,045 A→C 7.9% T524P (ACC→CCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 13.5% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 10.8% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 13.2% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,008 A→T 8.5% I37I (ATT→ATA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 780,640 T→G 6.3% T377P (ACG→CCG)  wcaC ← colanic acid biosynthesis glycosyltransferase WcaC
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 24.6% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,441 C→A 34.6% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 20.0% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 21.2% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 15.8% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,739 A→C 18.6% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 957,112 T→G 11.5% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 977,049 A→C 6.3% R635R (CGT→CGG rcsC ← two‑component system sensor histidine kinase RcsC
RA NZ_CP022686 1,005,085 C→A 15.4% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,034,493 T→G 7.0% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,065,984 C→A 10.7% V35F (GTC→TTC)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,065,997 A→C 14.3% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,959 T→G 10.7% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 15.8% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,197,147 T→G 8.5% L76R (CTG→CGG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,197,155 C→G 7.8% L79V (CTG→GTG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,213,479 T→G 13.3% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,269,551 T→G 7.9% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,360,724 G→T 9.5% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,729 G→T 7.5% intergenic (‑64/+260) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,742 C→A 11.7% intergenic (‑77/+247) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 9.4% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 10.1% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,392,487 A→C 8.6% T43P (ACG→CCG)  lhgO → L‑2‑hydroxyglutarate oxidase
RA NZ_CP022686 1,424,342 C→G 6.8% intergenic (‑38/+25) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,443,750 T→G 8.0% S164R (AGC→CGC)  hydN ← electron transport protein HydN
RA NZ_CP022686 1,479,625 G→C 9.9% intergenic (‑34/+159) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,257 A→T 14.6% S232C (AGT→TGT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,354 C→A 13.3% A264D (GCT→GAT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 11.8% A264A (GCT→GCA) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 22.5% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 10.6% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,573 T→G 9.4% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,589 A→C 9.4% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,235 T→G 11.2% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,604,863 G→C 5.5% G378G (GGC→GGG lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,633,846 T→G 30.7% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,633,876 A→C 12.0% V243G (GTG→GGG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,633,901 T→G 7.7% T235P (ACG→CCG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,888 G→T 13.2% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,640,925 A→C 10.1% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 14.6% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 7.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 9.4% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,032 G→T 12.7% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,825,402 Δ1 bp 5.3% intergenic (‑786/+272) CIW80_RS09095 ← / ← yghD ABC transporter permease/GspM family type II secretion system protein YghD
RA NZ_CP022686 1,828,110 A→C 6.5% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,832,023 T→G 7.0% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,833,749 G→T 12.6% V466V (GTC→GTA gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,762 A→C 11.4% L462R (CTG→CGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,860,077 G→T 11.6% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,954,923 T→G 18.8% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,971,467 C→A 8.9% intergenic (+58/‑126) ebgR → / → ebgA transcriptional regulator EbgR/beta‑galactosidase subunit alpha
RA NZ_CP022686 2,011,244 T→G 8.0% A53A (GCA→GCC tdcD ← propionate kinase
RA NZ_CP022686 2,016,160 T→G 6.6% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,175 T→G 8.7% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 17.4% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 10.6% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,112 C→A 8.2% A248A (GCG→GCT deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,780 A→C 7.3% K383N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,061,804 A→C 9.5% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,070,788 A→C 7.7% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,076,554 T→G 9.8% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,097,295 G→T 5.3% E240* (GAA→TAA)  gltB → glutamate synthase large subunit
RA NZ_CP022686 2,123,241 A→C 10.0% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 8.3% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 9.6% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 15.1% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 11.1% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.6% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,652 A→C 6.5% N160T (AAC→ACC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,159,673 C→A 12.9% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,163,522 C→A 5.4% intergenic (‑17/+23) rrf ← / ← CIW80_RS10805 5S ribosomal RNA/tRNA‑Thr
RA NZ_CP022686 2,172,177 A→C 5.5% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,184,330 G→T 13.8% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,075 A→C 10.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 6.9% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,272,831 G→T 8.8% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,305,971 G→T 9.3% S222* (TCA→TAA)  CIW80_RS11625 ← fimbrial biogenesis usher protein
RA NZ_CP022686 2,316,671 A→C 5.6% G474G (GGT→GGG glgB ← 1,4‑alpha‑glucan branching enzyme
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,356,278 A→C 8.0% intergenic (‑22/‑199) tusA ← / → yhhQ sulfurtransferase TusA/7‑cyano‑7‑deazaguanine/7‑aminomethyl‑7‑ deazaguanine transporter
RA NZ_CP022686 2,357,291 A→C 12.1% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 17.1% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,383,165 G→T 5.6% intergenic (+56/+58) uspA → / ← CIW80_RS26230 universal stress protein UspA/hypothetical protein
RA NZ_CP022686 2,386,496 T→G 6.3% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,013 T→G 6.6% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,398,885 A→C 10.1% S203R (AGC→CGC)  hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,398,896 A→C 13.4% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,410,598 G→T 9.9% pseudogene (2834/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,448,912 G→T 6.5% A180S (GCG→TCG)  yhjV → aromatic amino acid transport family protein
RA NZ_CP022686 2,460,576 C→A 10.7% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 18.5% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 13.9% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 11.0% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,517,086 A→C 5.1% K832N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,521,063 C→A 6.6% S242Y (TCC→TAC)  lldP → L‑lactate permease
RA NZ_CP022686 2,558,864 T→G 7.2% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,558,878 G→T 6.3% L132F (TTG→TTT yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,559,398 T→G 7.2% N461T (AAC→ACC)  ligB ← NAD‑dependent DNA ligase LigB
RA NZ_CP022686 2,572,701 T→A 7.1% S193C (AGT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,705 A→C 11.5% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 10.4% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,422 C→G 5.0% G232G (GGG→GGC CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
RA NZ_CP022686 2,574,429 T→G 13.6% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 6.8% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,703,299 A→C 7.9% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,717,400 T→G 7.0% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,733,571 T→G 12.7% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,586 A→C 8.8% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,758,128 A→C 8.2% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,144 A→C 10.4% T46P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 8.2% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,769,196 A→C 14.3% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,799,502 C→A 5.8% L82F (TTG→TTT CIW80_RS14015 ← tripartite tricarboxylate transporter permease
RA NZ_CP022686 2,801,864 T→G 8.5% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,127 T→G 9.5% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,821,644 A→C 6.8% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 8.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 19.4% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,745 C→G 11.5% A20A (GCG→GCC fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 17.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 11.0% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,856 T→G 9.7% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 6.2% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,418 C→A 9.0% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,428 A→C 10.0% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,203 T→G 5.9% T221P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,937,218 C→A 8.6% E216* (GAA→TAA)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,961,325 A→C 6.4% intergenic (+103/‑270) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,981,612 A→C 8.6% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,992,831 A→C 7.7% K133T (AAA→ACA)  nfi → deoxyribonuclease V
RA NZ_CP022686 2,994,693 T→G 10.3% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,017,622 A→C 8.2% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 13.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,066,354 T→G 8.9% K134N (AAA→AAC CIW80_RS15265 ← hypothetical protein
RA NZ_CP022686 3,086,831 T→A 6.3% T709S (ACA→TCA)  uvrA ← excinuclease ABC subunit UvrA
RA NZ_CP022686 3,136,940 T→G 10.1% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,151,803 C→A 5.9% S156Y (TCC→TAC)  proP → glycine betaine/L‑proline transporter ProP
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 18.2% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,297,333 T→G 13.3% F828V (TTT→GTT)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,342 A→T 7.8% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 6.2% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,347 A→T 6.6% intergenic (‑163/‑37) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,650 A→C 6.3% V220G (GTC→GGC)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,721 A→T 6.9% I524N (ATC→AAC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,459,376 C→A 13.4% S64Y (TCT→TAT)  bglJ → DNA‑binding transcriptional activator BglJ
RA NZ_CP022686 3,461,127 A→C 12.4% noncoding (65/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 11.6% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,221 T→G 12.9% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,224 T→G 11.4% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 19.9% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 16.5% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,270 T→A 10.8% noncoding (43/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,528,218 A→C 7.8% D57A (GAC→GCC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,606,036 G→T 9.4% E250* (GAA→TAA)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,628,846 C→A 9.0% A312E (GCA→GAA)  aceF → pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
RA NZ_CP022686 3,652,079 T→G 11.5% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,082 T→G 9.5% intergenic (‑91/+21) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,083 C→G 6.1% intergenic (‑92/+20) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,655,540 A→C 8.3% F765V (TTT→GTT)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,703,648 A→C 9.4% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 15.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 6.8% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 8.1% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,776,894 G→T 6.5% L132F (TTG→TTT CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,776,914 C→A 7.4% T139K (ACA→AAA)  CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 10.9% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,265 T→A 8.2% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,868,846 T→G 14.0% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,304 A→C 9.7% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,376 T→G 10.9% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 10.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,697 A→C 8.2% V317G (GTG→GGG)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 8.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,640 A→C 8.8% Q83P (CAG→CCG)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 8.1% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 14.4% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,242 A→T 11.9% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,247 G→T 12.2% intergenic (+93/‑251) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,248 C→G 10.5% intergenic (+94/‑250) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 17.4% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 10.9% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,079,332 A→C 8.6% W169G (TGG→GGG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,079,363 A→C 10.7% A158A (GCT→GCG ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,213,260 T→G 9.9% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,213,266 A→T 8.3% L190F (TTA→TTT ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,435 G→C 14.1% intergenic (‑8/+27) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,287,499 T→G 7.1% noncoding (12/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,331,897 G→T 12.2% S303* (TCG→TAG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 14.1% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,297 T→A 7.8% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,324 C→A 14.9% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 16.9% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,461,801 C→A 5.9% intergenic (+155/‑15) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,485,614 T→G 9.7% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,571,417 T→G 8.3% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,632,182 T→G 11.3% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 6.4% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,655,834 A→C 7.1% R390R (CGT→CGG iroC ← salmochelin/enterobactin export ABC transporter IroC
RA NZ_CP022686 4,712,763 A→C 20.0% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 11.1% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 12.2% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 10.0% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,437 T→G 6.0% L49R (CTG→CGG)  CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,809,441 T→G 11.7% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,961 T→G 10.4% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,687 T→G 9.2% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,909,058 T→G 8.1% noncoding (46/132 nt) CIW80_RS24835 ← RtT sRNA
RA NZ_CP022686 4,918,846 C→A 10.3% Q387H (CAG→CAT adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,947,316 G→T 8.5% A36S (GCT→TCT)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA NZ_CP022686 4,951,545 A→C 13.6% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,886 A→C 11.0% P104P (CCT→CCG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,043,648 G→C 6.7% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,137 G→T 10.5% V1256V (GTG→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,215 T→G 12.4% N1282K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.3% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
JC pHH‑29_RBS 1,408 Δ79 bp 87.7% [ompA] [ompA]
JC pHH‑29_RBS 1,522 Δ41 bp 22.5% coding (24‑64/570 nt) Aldafermin (NGM282) →
RA pHH‑29_RBS 2,872 G→T 14.5% intergenic (‑451/+257) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,892 G→T 8.2% intergenic (‑471/+237) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,894 T→G 5.5% intergenic (‑473/+235) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,899 Δ1 bp 100% intergenic (‑478/+230) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,901 C→A 6.7% intergenic (‑480/+228) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,930 G→T 9.5% intergenic (‑509/+199) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,935 G→T 7.7% intergenic (‑514/+194) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,946 G→T 6.0% intergenic (‑525/+183) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,953 G→T 14.7% intergenic (‑532/+176) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,964 G→T 8.5% intergenic (‑543/+165) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,966 G→T 6.1% intergenic (‑545/+163) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,991 G→T 7.4% intergenic (‑570/+138) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,993 G→T 8.6% intergenic (‑572/+136) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 3,601:1 +A 33.3% intergenic (‑44/‑113) putative HTH domain protein (MarR‑like) ← / → uncharacterized protein –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 206–2618 3084–2957 340–2879 18222 [17959] [17438] 18321 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3899666 =198 (0.840)63 (0.310) 39/216 NT 27.9% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 154 (0.750)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681194 (0.820)60 (0.290) 42/216 NT 27.1% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 154 (0.750)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA