breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 37,785 T→G 6.5% K3N (AAA→AAC ansP ← L‑asparagine permease
RA NZ_CP022686 119,184 T→G 11.1% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 197,854 T→G 12.7% V470G (GTT→GGT)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 298,027 A→C 12.4% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 350,056 A→C 12.2% K241T (AAA→ACA)  yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,290 T→G 12.1% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 10.6% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 418,374 T→G 13.7% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 513,980 A→C 9.4% R56R (CGT→CGG tcyL ← cystine ABC transporter permease
RA NZ_CP022686 556,942 G→T 16.5% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 21.1% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 28.9% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 606,098 T→G 11.1% R45R (CGT→CGG ybtU → yersiniabactin biosynthesis oxidoreductase YbtU
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 12.1% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,014 A→T 10.6% V35V (GTT→GTA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 21.3% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 18.0% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,080 G→T 20.7% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,105 A→C 12.1% intergenic (+174/‑83) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,005,085 C→A 21.5% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,909 A→C 11.3% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,065,997 A→C 11.7% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 16.5% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,209,908 T→G 19.3% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,209,931 C→A 11.5% A388A (GCG→GCT eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 13.1% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,360,709 T→G 9.0% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 10.2% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,081 C→A 18.2% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,479,607 A→C 11.1% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,257 A→T 10.8% S232C (AGT→TGT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,324 C→A 17.2% P254H (CCT→CAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,353 G→C 9.7% A264P (GCT→CCT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 31.4% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,110 G→C 19.4% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,564,543 A→C 9.2% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,246 C→G 11.0% G15A (GGC→GCC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,633,846 T→G 27.4% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 13.8% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 14.7% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,710,453 A→C 11.0% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,145 A→C 12.7% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 13.7% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 7.7% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,833,749 G→T 15.6% V466V (GTC→GTA gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,954,923 T→G 20.1% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,954,928 A→T 8.4% I90N (ATC→AAC)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,964,332 A→C 8.7% G27G (GGT→GGG mug ← G/U mismatch‑specific DNA glycosylase
RA NZ_CP022686 1,974,549 T→G 7.5% V986G (GTC→GGC)  ebgA → beta‑galactosidase subunit alpha
RA NZ_CP022686 2,016,160 T→G 6.5% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,225 T→G 12.3% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 16.1% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,554 T→G 11.6% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 11.9% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 12.8% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 12.2% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 12.1% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,075 A→C 8.4% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,284 G→C 8.9% D24H (GAC→CAC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 14.9% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,394,287 T→G 17.2% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,428,955 T→G 6.4% D106A (GAC→GCC)  yhjJ ← pitrilysin family protein
RA NZ_CP022686 2,460,576 C→A 14.7% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 9.6% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,502,553 A→C 41.2% I539S (ATC→AGC)  selB ← selenocysteine‑specific translation elongation factor
RA NZ_CP022686 2,516,873 A→C 7.0% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 8.9% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,717,400 T→G 6.6% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,743,563 T→G 13.2% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,769,196 A→C 15.1% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,801,817 T→G 8.1% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,123 C→A 8.0% Q440K (CAG→AAG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,808,145 T→G 10.3% V447G (GTG→GGG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,834,886 A→C 9.0% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 23.3% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 18.3% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,852 T→G 8.0% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 9.3% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,961,318 A→C 7.6% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 3,080,461 T→G 11.0% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,080,495 T→G 6.8% F170V (TTC→GTC)  CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,184,639 A→C 10.7% intergenic (‑86/+30) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
RA NZ_CP022686 3,184,651 A→C 10.6% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,096 G→T 13.7% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,348,371 T→G 8.2% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,614 G→T 11.4% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,451,845 A→C 8.8% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,229 T→G 15.1% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,509,248 T→G 47.6% L572R (CTG→CGG)  dnaK → molecular chaperone DnaK
RA NZ_CP022686 3,604,564 G→T 13.6% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,607,152 T→A 27.5% W622R (TGG→AGG)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 21.4% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,497 C→G 15.9% S135C (TCT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,111 C→A 8.4% R340S (CGT→AGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,925,279 T→A 9.6% Y153N (TAC→AAC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 15.7% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 13.5% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,724 A→C 8.3% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 11.3% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,969,137 A→C 5.4% R122R (CGT→CGG rdgC ← recombination‑associated protein RdgC
RA NZ_CP022686 3,977,646 G→T 12.7% R85L (CGA→CTA)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 9.9% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 10.4% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 14.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,287,491 T→G 8.1% noncoding (4/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,301,920 T→G 8.6% V136G (GTT→GGT)  modB → molybdate ABC transporter permease subunit
RA NZ_CP022686 4,368,341 T→G 14.3% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 13.7% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,324 C→A 10.8% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,589,367 T→G 10.8% T247P (ACC→CCC)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,616,703 T→G 13.2% intergenic (+185/+348) CIW80_RS23075 → / ← CIW80_RS26560 hypothetical protein/ATP‑binding protein
RA NZ_CP022686 4,713,008 A→C 7.6% intergenic (‑90/+44) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,839,682 C→G 10.3% E146Q (GAA→CAA)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 11.4% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,894,031 T→G 7.1% G172G (GGT→GGG narK → nitrate transporter NarK
RA NZ_CP022686 4,951,545 A→C 15.4% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,041,191 G→A 5.9% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 8.0% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,839 G→T 11.2% intergenic (‑204/+370) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,841 G→T 13.3% intergenic (‑206/+368) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,868 Δ1 bp 100% intergenic (‑233/+341) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,922 G→T 10.4% intergenic (‑287/+287) annotated as hok_sok locus ← / ← putative HTH domain protein –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 684–2618 3043–2957 340–2360 5906 [5473] [5642] 5934 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 329060 =120 (0.710)11 (0.070) 9/248 NT 8.5% intergenic (‑8/‑153) ydjA/sppA NAD(P)H nitroreductase/signal peptide peptidase SppA
?NZ_CP022686 = 1524129 NA (NA)intergenic (+7/+233) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 329063119 (0.700)8 (0.050) 7/250 NT 6.3% intergenic (‑11/‑150) ydjA/sppA NAD(P)H nitroreductase/signal peptide peptidase SppA
?NZ_CP022686 1522881 = NA (NA)intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
* ? NZ_CP022686 1123563 =120 (0.710)58 (0.340) 35/250 NT 32.6% coding (4434/7950 nt) CIW80_RS05650 phase‑variable autotransporter adhesin UpaE
?NZ_CP022686 1522881 = NA (NA)intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
* ? NZ_CP022686 = 1123565123 (0.720)34 (0.200) 20/250 NT 21.7% coding (4432/7950 nt) CIW80_RS05650 phase‑variable autotransporter adhesin UpaE
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 3395241NA (NA)5 (0.000)
+21 bp
4/208 NT 31.6% pseudogene (11/1107 nt) yjhR helicase YjhR
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 4514374 =191 (1.120)6 (0.000)
+AGCT
5/242 NT 5.7% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 13 (0.000)coding (1/399 nt) IL2wt no signal