breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_CP022686 | 47,354 | G→T | 7.8% | P665P (CCG→CCT) | vgrG → | type VI secretion system tip protein TssI/VgrG |
RA | NZ_CP022686 | 119,184 | T→G | 6.5% | F47V (TTT→GTT) | marB → | multiple antibiotic resistance protein MarB |
RA | NZ_CP022686 | 196,875 | A→C | 5.6% | N144H (AAC→CAC) | dtpA → | dipeptide/tripeptide permease DtpA |
RA | NZ_CP022686 | 238,317 | G→T | 18.5% | L88I (CTT→ATT) | sufE ← | cysteine desulfuration protein SufE |
RA | NZ_CP022686 | 263,831 | A→C | 12.9% | intergenic (+38/+19) | fadK → / ← ppsA | medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase |
RA | NZ_CP022686 | 298,027 | A→C | 10.9% | F60C (TTT→TGT) | chbC ← | PTS N,N'‑diacetylchitobiose transporter subunit IIC |
RA | NZ_CP022686 | 349,997 | C→A | 10.3% | A221A (GCC→GCA) | yeaH → | YeaH/YhbH family protein |
RA | NZ_CP022686 | 360,269 | T→G | 8.0% | T21P (ACT→CCT) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | NZ_CP022686 | 362,808 | C→A | 7.5% | intergenic (‑10/+72) | rnd ← / ← fadD | ribonuclease D/long‑chain‑fatty‑acid‑‑CoA ligase FadD |
RA | NZ_CP022686 | 372,574 | A→C | 18.8% | N275T (AAC→ACC) | sdaA → | L‑serine ammonia‑lyase |
RA | NZ_CP022686 | 377,274 | A→C | 12.0% | K136N (AAA→AAC) | manX → | PTS mannose transporter subunit IIAB |
RA | NZ_CP022686 | 418,374 | T→G | 7.1% | F48V (TTT→GTT) | znuB → | zinc ABC transporter permease subunit ZnuB |
RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
RA | NZ_CP022686 | 487,535 | T→G | 6.2% | Q534H (CAA→CAC) | tar ← | methyl‑accepting chemotaxis protein II |
RA | NZ_CP022686 | 556,940 | A→T | 13.8% | intergenic (+124/‑724) | zinT → / → CIW80_RS03080 | metal‑binding protein ZinT/site‑specific integrase |
RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
RA | NZ_CP022686 | 582,870 | C→A | 11.9% | W401C (TGG→TGT) | ybtS ← | yersiniabactin biosynthesis salicylate synthase Irp9/YbtS |
RA | NZ_CP022686 | 601,295 | T→G | 17.5% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 601,302 | T→G | 22.6% | R1609R (CGT→CGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
RA | NZ_CP022686 | 632,723 | A→C | 5.8% | F1304V (TTT→GTT) | clbN ← | colibactin non‑ribosomal peptide synthetase ClbN |
RA | NZ_CP022686 | 636,727 | A→C | 9.3% | L448V (TTG→GTG) | clbM ← | precolibactin export MATE transporter ClbM |
RA | NZ_CP022686 | 651,833 | A→C | 12.3% | P243P (CCT→CCG) | clbJ ← | colibactin non‑ribosomal peptide synthetase ClbJ |
RA | NZ_CP022686 | 651,848 | A→C | 15.4% | R238R (CGT→CGG) | clbJ ← | colibactin non‑ribosomal peptide synthetase ClbJ |
RA | NZ_CP022686 | 694,391 | C→A | 8.3% | intergenic (+151/‑45) | CIW80_RS03555 → / → CIW80_RS03560 | transposase/PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 694,406 | G→T | 12.3% | intergenic (+166/‑30) | CIW80_RS03555 → / → CIW80_RS03560 | transposase/PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 694,421 | G→T | 10.5% | intergenic (+181/‑15) | CIW80_RS03555 → / → CIW80_RS03560 | transposase/PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
RA | NZ_CP022686 | 764,997 | T→G | 19.0% | N41T (AAC→ACC) | wcaM ← | colanic acid biosynthesis protein WcaM |
RA | NZ_CP022686 | 777,349 | C→A | 11.2% | M221I (ATG→ATT) | gmd ← | GDP‑mannose 4,6‑dehydratase |
RA | NZ_CP022686 | 777,367 | G→C | 5.6% | C215W (TGC→TGG) | gmd ← | GDP‑mannose 4,6‑dehydratase |
RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
RA | NZ_CP022686 | 829,435 | C→A | 15.2% | intergenic (‑24/+203) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,441 | C→A | 25.3% | intergenic (‑30/+197) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,452 | G→T | 15.8% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,467 | C→A | 16.8% | intergenic (‑56/+171) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,472 | G→T | 10.6% | intergenic (‑61/+166) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 837,573 | C→A | 16.0% | intergenic (‑146/+77) | thiM ← / ← rcnR | hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR |
RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
RA | NZ_CP022686 | 948,023 | G→T | 17.9% | intergenic (+92/‑165) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,049 | A→T | 7.8% | intergenic (+118/‑139) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,051 | G→T | 10.9% | intergenic (+120/‑137) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,080 | G→T | 16.8% | intergenic (+149/‑108) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,090 | C→A | 21.4% | intergenic (+159/‑98) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 957,112 | T→G | 20.7% | T89P (ACC→CCC) | ccmA ← | cytochrome c biogenesis heme‑transporting ATPase CcmA |
RA | NZ_CP022686 | 1,005,085 | C→A | 15.6% | T380T (ACC→ACA) | nrdA → | ribonucleoside‑diphosphate reductase subunit alpha |
RA | NZ_CP022686 | 1,011,419 | T→A | 11.3% | intergenic (‑268/‑5) | glpT ← / → glpA | glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A |
RA | NZ_CP022686 | 1,034,493 | T→G | 9.4% | T26P (ACC→CCC) | menC ← | o‑succinylbenzoate synthase |
RA | NZ_CP022686 | 1,040,909 | A→C | 12.9% | E232D (GAA→GAC) | rbn → | ribonuclease BN |
RA | NZ_CP022686 | 1,100,944 | T→G | 15.8% | D187A (GAC→GCC) | aroC ← | chorismate synthase |
RA | NZ_CP022686 | 1,174,272 | A→C | 17.9% | intergenic (+1/‑43) | CIW80_RS05855 → / → CIW80_RS05860 | tRNA‑Val/tRNA‑Val |
RA | NZ_CP022686 | 1,213,483 | T→G | 12.7% | N419T (AAC→ACC) | eutE ← | aldehyde dehydrogenase EutE |
RA | NZ_CP022686 | 1,237,910 | A→C | 6.4% | F306V (TTT→GTT) | tmcA ← | tRNA cytosine(34) acetyltransferase TmcA |
RA | NZ_CP022686 | 1,239,833 | G→T | 14.1% | intergenic (‑130/+38) | ypfJ ← / ← purC | neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase |
RA | NZ_CP022686 | 1,280,117 | T→G | 9.2% | N8H (AAC→CAC) | yfgM ← | ancillary SecYEG translocon subunit YfgM |
RA | NZ_CP022686 | 1,360,709 | T→G | 11.9% | intergenic (‑44/+280) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,724 | G→T | 16.4% | intergenic (‑59/+265) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,727 | A→T | 6.8% | intergenic (‑62/+262) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,742 | C→A | 9.9% | intergenic (‑77/+247) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,750 | G→T | 11.8% | intergenic (‑85/+239) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,370,559 | T→G | 13.6% | K98N (AAA→AAC) | rluD ← | 23S rRNA pseudouridine(1911/1915/1917) synthase RluD |
RA | NZ_CP022686 | 1,371,081 | C→A | 18.6% | P32Q (CCA→CAA) | bamD → | outer membrane protein assembly factor BamD |
RA | NZ_CP022686 | 1,372,377 | T→G | 13.9% | intergenic (+42/‑62) | raiA → / → pheL | ribosome‑associated translation inhibitor RaiA/pheA operon leader peptide PheL |
RA | NZ_CP022686 | 1,383,405 | T→G | 5.3% | F240V (TTT→GTT) | ypjD → | inner membrane protein YpjD |
RA | NZ_CP022686 | 1,396,426 | T→G | 9.7% | intergenic (+32/‑205) | gabT → / → gabP | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease |
RA | NZ_CP022686 | 1,396,432 | C→G | 6.9% | intergenic (+38/‑199) | gabT → / → gabP | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease |
RA | NZ_CP022686 | 1,479,607 | A→C | 11.9% | intergenic (‑16/+177) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
RA | NZ_CP022686 | 1,500,012 | C→A | 12.4% | P257T (CCG→ACG) | ygcE → | FGGY‑family carbohydrate kinase |
RA | NZ_CP022686 | 1,504,310 | G→T | 8.9% | M249I (ATG→ATT) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,351 | A→C | 10.2% | N263T (AAC→ACC) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,355 | T→A | 11.7% | A264A (GCT→GCA) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,359 | G→T | 11.6% | V266L (GTG→TTG) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,547,000 | G→C | 10.2% | intergenic (+22/‑12) | CIW80_RS07705 → / → CIW80_RS07710 | tRNA‑Met/tRNA‑Met |
RA | NZ_CP022686 | 1,547,003 | G→C | 10.0% | intergenic (+25/‑9) | CIW80_RS07705 → / → CIW80_RS07710 | tRNA‑Met/tRNA‑Met |
RA | NZ_CP022686 | 1,558,395 | A→C | 12.7% | K464N (AAA→AAC) | vgrG → | type VI secretion system Vgr family protein |
RA | NZ_CP022686 | 1,564,569 | A→C | 10.7% | K190N (AAA→AAC) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,564,589 | A→C | 11.3% | N197T (AAC→ACC) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,566,217 | A→C | 14.4% | T740P (ACG→CCG) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,604,828 | A→C | 8.8% | L390R (CTG→CGG) | lplT ← | lysophospholipid transporter LplT |
RA | NZ_CP022686 | 1,632,501 | T→G | 11.0% | V166G (GTT→GGT) | arcC → | carbamate kinase |
RA | NZ_CP022686 | 1,633,846 | T→G | 26.8% | N253T (AAC→ACC) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
RA | NZ_CP022686 | 1,633,870 | G→T | 9.5% | A245E (GCA→GAA) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
RA | NZ_CP022686 | 1,640,888 | G→T | 9.7% | intergenic (+34/‑17) | ssnA → / → ygfM | putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
RA | NZ_CP022686 | 1,665,247 | G→T | 8.0% | V445V (GTG→GTT) | bglA → | 6‑phospho‑beta‑glucosidase BglA |
RA | NZ_CP022686 | 1,674,224 | T→G | 13.4% | K188N (AAA→AAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,244 | T→G | 12.5% | T182P (ACC→CCC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,248 | T→G | 13.3% | Q180H (CAA→CAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,706,025 | C→A | 16.5% | intergenic (‑635/‑20) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,706,047 | G→T | 11.4% | M1M (ATG→ATT) † | metK → | methionine adenosyltransferase |
RA | NZ_CP022686 | 1,805,014 | G→T | 11.2% | pseudogene (295/934 nt) | CIW80_RS09020 → | nucleotidyl transferase AbiEii/AbiGii toxin family protein |
RA | NZ_CP022686 | 1,828,110 | A→C | 7.8% | R85R (CGT→CGG) | gspK ← | type II secretion system minor pseudopilin GspK |
RA | NZ_CP022686 | 1,860,070 | C→A | 18.7% | E119* (GAA→TAA) | CIW80_RS09255 ← | YtfJ family protein |
RA | NZ_CP022686 | 1,925,332 | A→C | 13.0% | N151T (AAC→ACC) | ygiB → | DUF1190 family protein |
RA | NZ_CP022686 | 1,925,337 | G→C | 9.1% | A153P (GCC→CCC) | ygiB → | DUF1190 family protein |
RA | NZ_CP022686 | 1,965,147 | A→T | 8.5% | L95Q (CTA→CAA) | nfeF ← | NADPH‑dependent ferric chelate reductase |
RA | NZ_CP022686 | 2,016,170 | A→C | 12.2% | noncoding (104/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,016,175 | T→G | 6.6% | noncoding (99/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,049,094 | G→T | 9.6% | T254T (ACC→ACA) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,061,804 | A→C | 8.9% | K391N (AAA→AAC) | argG → | argininosuccinate synthase |
RA | NZ_CP022686 | 2,070,788 | A→C | 10.6% | intergenic (‑80/‑169) | greA ← / → dacB | transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase |
RA | NZ_CP022686 | 2,110,461 | A→T | 8.6% | V11E (GTA→GAA) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,110,472 | T→G | 7.2% | K7N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,123,241 | A→C | 12.5% | F208V (TTT→GTT) | tldD ← | metalloprotease TldD |
RA | NZ_CP022686 | 2,130,829 | A→C | 9.7% | V237G (GTT→GGT) | mreC ← | rod shape‑determining protein MreC |
RA | NZ_CP022686 | 2,148,115 | T→G | 11.1% | L126R (CTG→CGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,136 | T→G | 7.2% | V133G (GTT→GGT) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,159,673 | C→A | 13.2% | A167E (GCA→GAA) | CIW80_RS10780 → | amino acid ABC transporter substrate‑binding protein |
RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
RA | NZ_CP022686 | 2,226,139 | A→C | 7.8% | D9A (GAC→GCC) | crp → | cAMP‑activated global transcriptional regulator CRP |
RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
RA | NZ_CP022686 | 2,357,291 | A→C | 13.4% | N26T (AAC→ACC) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,380,936 | A→C | 9.7% | K186N (AAA→AAC) | pitA → | inorganic phosphate transporter PitA |
RA | NZ_CP022686 | 2,394,295 | G→T | 9.2% | I112I (ATC→ATA) | chuS ← | hematinate‑forming heme oxygenase ChuS |
RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
RA | NZ_CP022686 | 2,433,306 | A→C | 8.5% | R1066R (CGT→CGG) | bcsC ← | cellulose synthase complex outer membrane protein BcsC |
RA | NZ_CP022686 | 2,460,581 | A→C | 21.5% | intergenic (‑3/‑155) | yhjY ← / → tag | autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I |
RA | NZ_CP022686 | 2,470,422 | T→G | 13.6% | K72N (AAA→AAC) | glyS ← | glycine‑‑tRNA ligase subunit beta |
RA | NZ_CP022686 | 2,501,922 | T→G | 6.3% | N71T (AAC→ACC) | yiaY ← | L‑threonine dehydrogenase |
RA | NZ_CP022686 | 2,516,426 | T→C | 7.6% | G612G (GGT→GGC) | upaG → | trimeric autotransporter adhesin UpaG |
RA | NZ_CP022686 | 2,546,400 | A→C | 13.1% | L93R (CTG→CGG) | waaG ← | glycosyltransferase family 4 protein |
RA | NZ_CP022686 | 2,558,874 | T→G | 5.6% | V131G (GTT→GGT) | yicG → | trimeric intracellular cation channel family protein |
RA | NZ_CP022686 | 2,572,705 | A→C | 10.0% | G191G (GGT→GGG) | CIW80_RS12885 ← | ROK family protein |
RA | NZ_CP022686 | 2,572,721 | A→C | 9.7% | F186C (TTT→TGT) | CIW80_RS12885 ← | ROK family protein |
RA | NZ_CP022686 | 2,574,429 | T→G | 8.3% | N230T (AAC→ACC) | CIW80_RS12895 ← | class II fructose‑bisphosphate aldolase family protein |
JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
RA | NZ_CP022686 | 2,624,283 | A→C | 7.4% | L143V (TTG→GTG) | yidK ← | solute:sodium symporter family transporter |
RA | NZ_CP022686 | 2,733,571 | T→G | 10.8% | F53V (TTT→GTT) | rffG → | dTDP‑glucose 4,6‑dehydratase |
RA | NZ_CP022686 | 2,736,578 | C→A | 10.6% | T180T (ACC→ACA) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
RA | NZ_CP022686 | 2,758,128 | A→C | 7.6% | K40N (AAA→AAC) | yifL → | lipoprotein |
RA | NZ_CP022686 | 2,780,110 | T→G | 9.8% | F677C (TTT→TGT) | metE → | 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase |
RA | NZ_CP022686 | 2,834,886 | A→C | 9.4% | K597N (AAA→AAC) | polA → | DNA polymerase I |
RA | NZ_CP022686 | 2,834,898 | A→C | 11.0% | K601N (AAA→AAC) | polA → | DNA polymerase I |
RA | NZ_CP022686 | 2,868,740 | T→G | 18.9% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,868,753 | T→G | 8.6% | T18P (ACC→CCC) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,871,422 | T→G | 8.0% | N149T (AAC→ACC) | fdnG ← | formate dehydrogenase‑N subunit alpha |
RA | NZ_CP022686 | 2,904,856 | T→G | 6.2% | K237N (AAA→AAC) | ftsN ← | cell division protein FtsN |
RA | NZ_CP022686 | 2,961,318 | A→C | 9.5% | intergenic (+96/‑277) | murI → / → CIW80_RS14765 | glutamate racemase/16S ribosomal RNA |
RA | NZ_CP022686 | 3,020,229 | T→G | 9.0% | V133G (GTC→GGC) | yjbB → | Na/Pi cotransporter family protein |
RA | NZ_CP022686 | 3,184,651 | A→C | 8.4% | intergenic (‑98/+18) | cutA ← / ← dcuA | divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA |
JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
RA | NZ_CP022686 | 3,213,199 | A→C | 10.4% | T273P (ACG→CCG) | nnr → | bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase |
RA | NZ_CP022686 | 3,218,260 | T→G | 9.9% | L233R (CTT→CGT) | miaA → | tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA |
RA | NZ_CP022686 | 3,278,016 | G→C | 8.2% | D286H (GAC→CAC) | ytfQ → | galactofuranose ABC transporter substrate‑binding protein YtfQ |
RA | NZ_CP022686 | 3,281,052 | T→G | 11.2% | F91V (TTT→GTT) | yjfF → | sugar ABC transporter permease YjfF |
RA | NZ_CP022686 | 3,287,019 | A→C | 8.7% | E108D (GAA→GAC) | cybC → | cytochrome b562 |
RA | NZ_CP022686 | 3,287,046 | A→C | 7.2% | K117N (AAA→AAC) | cybC → | cytochrome b562 |
RA | NZ_CP022686 | 3,297,360 | T→G | 11.1% | L837V (TTA→GTA) | mgtA → | magnesium‑translocating P‑type ATPase |
RA | NZ_CP022686 | 3,348,357 | T→G | 5.9% | intergenic (‑173/‑27) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,387,650 | A→C | 6.5% | V220G (GTC→GGC) | sgcQ ← | BtpA family protein SgcQ |
RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
RA | NZ_CP022686 | 3,444,704 | T→G | 14.1% | T530P (ACC→CCC) | btsT ← | pyruvate/proton symporter BtsT |
RA | NZ_CP022686 | 3,461,202 | T→A | 17.0% | intergenic (‑11/+24) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
RA | NZ_CP022686 | 3,461,223 | G→T | 10.1% | intergenic (‑32/+3) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
RA | NZ_CP022686 | 3,461,229 | T→G | 18.4% | noncoding (84/87 nt) | CIW80_RS17340 ← | tRNA‑Leu |
RA | NZ_CP022686 | 3,625,683 | T→G | 11.0% | A150A (GCT→GCG) | aceE → | pyruvate dehydrogenase (acetyl‑transferring), homodimeric type |
RA | NZ_CP022686 | 3,650,128 | T→G | 9.8% | V25G (GTT→GGT) | CIW80_RS18240 → | hypothetical protein |
RA | NZ_CP022686 | 3,652,079 | T→G | 11.1% | intergenic (‑88/+24) | panB ← / ← CIW80_RS18255 | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin |
RA | NZ_CP022686 | 3,652,090 | T→G | 8.1% | intergenic (‑99/+13) | panB ← / ← CIW80_RS18255 | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin |
RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
RA | NZ_CP022686 | 3,703,648 | A→C | 5.1% | T120P (ACG→CCG) | fabZ → | 3‑hydroxyacyl‑ACP dehydratase FabZ |
RA | NZ_CP022686 | 3,714,342 | T→G | 8.9% | L275V (TTG→GTG) | tilS → | tRNA lysidine(34) synthetase TilS |
RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
RA | NZ_CP022686 | 3,797,481 | T→G | 14.2% | L130V (TTA→GTA) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,797,493 | G→T | 12.6% | G134C (GGT→TGT) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,800,981 | A→C | 10.1% | pseudogene (1257/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
RA | NZ_CP022686 | 3,801,492 | T→G | 5.5% | pseudogene (1768/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
RA | NZ_CP022686 | 3,824,319 | T→G | 9.1% | L213R (CTT→CGT) | CIW80_RS19155 → | TolC family protein |
RA | NZ_CP022686 | 3,852,459 | T→G | 8.6% | N178T (AAC→ACC) | phoE ← | phosphoporin PhoE |
RA | NZ_CP022686 | 3,895,534 | T→G | 8.9% | intergenic (‑140/+44) | CIW80_RS19510 ← / ← CIW80_RS19515 | protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein |
RA | NZ_CP022686 | 3,914,747 | A→C | 8.8% | D132A (GAC→GCC) | yahG → | DUF1116 domain‑containing protein |
RA | NZ_CP022686 | 3,925,282 | A→C | 11.4% | S154R (AGC→CGC) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,925,289 | A→C | 15.6% | N156T (AAC→ACC) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,928,345 | T→G | 11.5% | F277V (TTC→GTC) | prpE → | propionate‑‑CoA ligase |
RA | NZ_CP022686 | 3,928,369 | T→G | 8.3% | W285G (TGG→GGG) | prpE → | propionate‑‑CoA ligase |
RA | NZ_CP022686 | 3,935,324 | T→G | 8.8% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
RA | NZ_CP022686 | 3,940,707 | A→C | 8.6% | F314V (TTT→GTT) | frmA ← | S‑(hydroxymethyl)glutathione dehydrogenase |
RA | NZ_CP022686 | 3,942,556 | T→G | 6.8% | T121P (ACG→CCG) | yaiO ← | YaiO family outer membrane beta‑barrel protein |
RA | NZ_CP022686 | 3,949,969 | T→G | 10.8% | D141A (GAC→GCC) | hemB ← | porphobilinogen synthase |
RA | NZ_CP022686 | 3,950,011 | T→G | 7.0% | H127P (CAC→CCC) | hemB ← | porphobilinogen synthase |
RA | NZ_CP022686 | 3,977,656 | A→C | 8.9% | K88N (AAA→AAC) | phoR → | phosphate regulon sensor histidine kinase PhoR |
RA | NZ_CP022686 | 3,981,933 | T→G | 14.9% | intergenic (+70/‑86) | proY → / → malZ | proline‑specific permease ProY/maltodextrin glucosidase |
RA | NZ_CP022686 | 4,014,238 | C→G | 12.8% | intergenic (+84/‑260) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,014,242 | A→T | 11.1% | intergenic (+88/‑256) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,014,248 | C→G | 10.4% | intergenic (+94/‑250) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,014,250 | C→G | 10.1% | intergenic (+96/‑248) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,025,356 | T→G | 8.2% | N445T (AAC→ACC) | ybaE ← | SgrR family transcriptional regulator |
RA | NZ_CP022686 | 4,056,941 | T→G | 9.1% | G130G (GGT→GGG) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,056,962 | C→A | 20.4% | F137L (TTC→TTA) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,079,332 | A→C | 11.7% | W169G (TGG→GGG) | ybbO ← | NADP(+)‑dependent aldehyde reductase |
RA | NZ_CP022686 | 4,214,457 | G→T | 9.3% | intergenic (‑30/+5) | CIW80_RS21095 ← / ← CIW80_RS21100 | tRNA‑Gln/tRNA‑Gln |
RA | NZ_CP022686 | 4,287,456 | T→G | 12.3% | noncoding (47/76 nt) | CIW80_RS21475 → | tRNA‑Val |
RA | NZ_CP022686 | 4,290,599 | T→G | 6.1% | T121P (ACC→CCC) | ybgS ← | YbgS‑like family protein |
RA | NZ_CP022686 | 4,327,145 | G→T | 6.4% | L189F (TTG→TTT) | ybhL → | Bax inhibitor‑1/YccA family protein |
RA | NZ_CP022686 | 4,368,341 | T→G | 13.8% | D47A (GAC→GCC) | ybiY ← | glycyl‑radical enzyme activating protein |
RA | NZ_CP022686 | 4,412,274 | A→C | 7.3% | V244G (GTG→GGG) | ybjT ← | SDR family oxidoreductase |
RA | NZ_CP022686 | 4,412,284 | A→C | 10.5% | W241G (TGG→GGG) | ybjT ← | SDR family oxidoreductase |
RA | NZ_CP022686 | 4,440,324 | C→A | 11.5% | P794Q (CCG→CAG) | ftsK → | DNA translocase FtsK |
RA | NZ_CP022686 | 4,632,186 | G→T | 5.8% | G278C (GGC→TGC) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
RA | NZ_CP022686 | 4,642,951 | A→C | 7.2% | N732T (AAC→ACC) | sfaF → | S/F1C fimbrial usher SfaF/FocD |
RA | NZ_CP022686 | 4,642,954 | A→C | 6.8% | Q733P (CAG→CCG) | sfaF → | S/F1C fimbrial usher SfaF/FocD |
RA | NZ_CP022686 | 4,661,469 | C→A | 8.9% | pseudogene (569/655 nt) | CIW80_RS26570 → | hypothetical protein |
RA | NZ_CP022686 | 4,678,041 | A→C | 17.1% | intergenic (+39/‑82) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
RA | NZ_CP022686 | 4,678,047 | A→C | 21.6% | intergenic (+45/‑76) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
RA | NZ_CP022686 | 4,700,039 | C→A | 5.0% | P354T (CCA→ACA) | mdoG → | glucans biosynthesis protein MdoG |
RA | NZ_CP022686 | 4,712,763 | A→C | 13.5% | A52A (GCT→GCG) | yceB ← | lipoprotein |
RA | NZ_CP022686 | 4,712,985 | A→C | 5.8% | intergenic (‑67/+67) | yceB ← / ← grxB | lipoprotein/glutaredoxin 2 |
RA | NZ_CP022686 | 4,764,877 | T→G | 14.5% | intergenic (‑57/‑204) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,764,883 | A→T | 11.5% | intergenic (‑63/‑198) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,783,330 | T→G | 11.6% | E33D (GAA→GAC) | mnmA ← | tRNA 2‑thiouridine(34) synthase MnmA |
RA | NZ_CP022686 | 4,814,990 | T→G | 11.2% | F71V (TTT→GTT) | CIW80_RS24295 → | phage tail protein |
RA | NZ_CP022686 | 4,815,007 | T→G | 9.7% | P76P (CCT→CCG) | CIW80_RS24295 → | phage tail protein |
RA | NZ_CP022686 | 4,815,886 | T→G | 7.8% | V25G (GTG→GGG) | CIW80_RS24305 → | C40 family peptidase |
RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
RA | NZ_CP022686 | 4,905,178 | T→G | 15.2% | F102V (TTC→GTC) | CIW80_RS24815 → | coenzyme F390 synthetase |
RA | NZ_CP022686 | 4,918,999 | T→G | 7.7% | K336N (AAA→AAC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
RA | NZ_CP022686 | 4,951,521 | A→C | 12.4% | T39P (ACC→CCC) | topA → | type I DNA topoisomerase |
RA | NZ_CP022686 | 4,951,547 | T→G | 19.2% | S47R (AGT→AGG) | topA → | type I DNA topoisomerase |
RA | NZ_CP022686 | 4,980,895 | A→C | 10.5% | C101W (TGT→TGG) | sapD ← | peptide ABC transporter ATP‑binding protein SapD |
RA | NZ_CP022686 | 5,023,952 | G→T | 6.2% | intergenic (+218/‑260) | zntB → / → dbpA | zinc transporter ZntB/ATP‑dependent RNA helicase DbpA |
RA | NZ_CP022686 | 5,043,831 | C→T | 6.1% | D1154D (GAC→GAT) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,044,137 | G→T | 17.7% | V1256V (GTG→GTT) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_MW240712 | 189–2618 | 3084–2957 | 340–2896 | 8275 [7602] | [7622] 8246 | JNG15_RS00005–mobA | JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA |
* | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4740–4810 | 5417–5074 | 265–678 | 4034 [3796] | [3528] 4037 | [putative HTH domain protein] | [putative HTH domain protein] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP022686 | 1123563 = | 154 (0.730) | 60 (0.280) | 42/250 | NT | 28.0% | coding (4434/7950 nt) | CIW80_RS05650 | phase‑variable autotransporter adhesin UpaE |
? | NZ_CP022686 | 1522881 = | NA (NA) | intergenic (‑394/‑72) | yqcC/CIW80_RS07590 | YqcC family protein/IS3‑like element ISEc52 family transposase | |||||
* | ? | NZ_CP022686 | = 1123565 | 158 (0.750) | 44 (0.210) | 33/250 | NT | 21.8% | coding (4432/7950 nt) | CIW80_RS05650 | phase‑variable autotransporter adhesin UpaE |
? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
* | ? | NZ_CP022686 | 3743172 = | 279 (1.330) | 15 (0.070) | 13/248 | NT | 5.3% | intergenic (+1/+253) | CIW80_RS18695/CIW80_RS18700 | IS66 family transposase/IS110‑like element ISEc45 family transposase |
? | NZ_CP022686 | = 3744618 | 255 (1.220) | intergenic (‑60/‑4) | CIW80_RS18700/CIW80_RS18705 | IS110‑like element ISEc45 family transposase/transposase |