breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 47,354 G→T 7.8% P665P (CCG→CCT vgrG → type VI secretion system tip protein TssI/VgrG
RA NZ_CP022686 119,184 T→G 6.5% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 196,875 A→C 5.6% N144H (AAC→CAC)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 238,317 G→T 18.5% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 12.9% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 10.9% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,997 C→A 10.3% A221A (GCC→GCA yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,269 T→G 8.0% T21P (ACT→CCT)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 362,808 C→A 7.5% intergenic (‑10/+72) rnd ← / ← fadD ribonuclease D/long‑chain‑fatty‑acid‑‑CoA ligase FadD
RA NZ_CP022686 372,574 A→C 18.8% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 377,274 A→C 12.0% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 418,374 T→G 7.1% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 487,535 T→G 6.2% Q534H (CAA→CAC tar ← methyl‑accepting chemotaxis protein II
RA NZ_CP022686 556,940 A→T 13.8% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 582,870 C→A 11.9% W401C (TGG→TGT ybtS ← yersiniabactin biosynthesis salicylate synthase Irp9/YbtS
RA NZ_CP022686 601,295 T→G 17.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.6% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 632,723 A→C 5.8% F1304V (TTT→GTT)  clbN ← colibactin non‑ribosomal peptide synthetase ClbN
RA NZ_CP022686 636,727 A→C 9.3% L448V (TTG→GTG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,833 A→C 12.3% P243P (CCT→CCG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 651,848 A→C 15.4% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,391 C→A 8.3% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,406 G→T 12.3% intergenic (+166/‑30) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,421 G→T 10.5% intergenic (+181/‑15) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 19.0% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 777,349 C→A 11.2% M221I (ATG→ATT gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 777,367 G→C 5.6% C215W (TGC→TGG gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 15.2% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,441 C→A 25.3% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 15.8% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.8% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 10.6% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,573 C→A 16.0% intergenic (‑146/+77) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,023 G→T 17.9% intergenic (+92/‑165) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 7.8% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,051 G→T 10.9% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 16.8% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 21.4% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 20.7% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 15.6% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,419 T→A 11.3% intergenic (‑268/‑5) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,034,493 T→G 9.4% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,040,909 A→C 12.9% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,100,944 T→G 15.8% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 17.9% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,213,483 T→G 12.7% N419T (AAC→ACC)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,910 A→C 6.4% F306V (TTT→GTT)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,239,833 G→T 14.1% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,280,117 T→G 9.2% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,360,709 T→G 11.9% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 16.4% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,727 A→T 6.8% intergenic (‑62/+262) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,742 C→A 9.9% intergenic (‑77/+247) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 11.8% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,559 T→G 13.6% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,081 C→A 18.6% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,372,377 T→G 13.9% intergenic (+42/‑62) raiA → / → pheL ribosome‑associated translation inhibitor RaiA/pheA operon leader peptide PheL
RA NZ_CP022686 1,383,405 T→G 5.3% F240V (TTT→GTT)  ypjD → inner membrane protein YpjD
RA NZ_CP022686 1,396,426 T→G 9.7% intergenic (+32/‑205) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,396,432 C→G 6.9% intergenic (+38/‑199) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,479,607 A→C 11.9% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,500,012 C→A 12.4% P257T (CCG→ACG)  ygcE → FGGY‑family carbohydrate kinase
RA NZ_CP022686 1,504,310 G→T 8.9% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,351 A→C 10.2% N263T (AAC→ACC)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 11.7% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,359 G→T 11.6% V266L (GTG→TTG)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,000 G→C 10.2% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,003 G→C 10.0% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 12.7% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 10.7% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,589 A→C 11.3% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,217 A→C 14.4% T740P (ACG→CCG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,604,828 A→C 8.8% L390R (CTG→CGG)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,632,501 T→G 11.0% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 26.8% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,633,870 G→T 9.5% A245E (GCA→GAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,888 G→T 9.7% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,665,247 G→T 8.0% V445V (GTG→GTT bglA → 6‑phospho‑beta‑glucosidase BglA
RA NZ_CP022686 1,674,224 T→G 13.4% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.5% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 13.3% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,025 C→A 16.5% intergenic (‑635/‑20) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,047 G→T 11.4% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,805,014 G→T 11.2% pseudogene (295/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,828,110 A→C 7.8% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,860,070 C→A 18.7% E119* (GAA→TAA)  CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,925,332 A→C 13.0% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,337 G→C 9.1% A153P (GCC→CCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,965,147 A→T 8.5% L95Q (CTA→CAA)  nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,170 A→C 12.2% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,175 T→G 6.6% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,094 G→T 9.6% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,804 A→C 8.9% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,070,788 A→C 10.6% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,110,461 A→T 8.6% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,472 T→G 7.2% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 12.5% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 9.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,115 T→G 11.1% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 7.2% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,673 C→A 13.2% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 7.8% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 13.4% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,380,936 A→C 9.7% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,394,295 G→T 9.2% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,433,306 A→C 8.5% R1066R (CGT→CGG bcsC ← cellulose synthase complex outer membrane protein BcsC
RA NZ_CP022686 2,460,581 A→C 21.5% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 13.6% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,501,922 T→G 6.3% N71T (AAC→ACC)  yiaY ← L‑threonine dehydrogenase
RA NZ_CP022686 2,516,426 T→C 7.6% G612G (GGT→GGC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,400 A→C 13.1% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,558,874 T→G 5.6% V131G (GTT→GGT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,705 A→C 10.0% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 9.7% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 8.3% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,624,283 A→C 7.4% L143V (TTG→GTG)  yidK ← solute:sodium symporter family transporter
RA NZ_CP022686 2,733,571 T→G 10.8% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,578 C→A 10.6% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,758,128 A→C 7.6% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,780,110 T→G 9.8% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,834,886 A→C 9.4% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,834,898 A→C 11.0% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 18.9% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 8.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 8.0% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,856 T→G 6.2% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,961,318 A→C 9.5% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 3,020,229 T→G 9.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,184,651 A→C 8.4% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,213,199 A→C 10.4% T273P (ACG→CCG)  nnr → bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase
RA NZ_CP022686 3,218,260 T→G 9.9% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,278,016 G→C 8.2% D286H (GAC→CAC)  ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,281,052 T→G 11.2% F91V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,287,019 A→C 8.7% E108D (GAA→GAC cybC → cytochrome b562
RA NZ_CP022686 3,287,046 A→C 7.2% K117N (AAA→AAC cybC → cytochrome b562
RA NZ_CP022686 3,297,360 T→G 11.1% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,357 T→G 5.9% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,650 A→C 6.5% V220G (GTC→GGC)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 14.1% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,202 T→A 17.0% intergenic (‑11/+24) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 10.1% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 18.4% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,625,683 T→G 11.0% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,650,128 T→G 9.8% V25G (GTT→GGT)  CIW80_RS18240 → hypothetical protein
RA NZ_CP022686 3,652,079 T→G 11.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 8.1% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,703,648 A→C 5.1% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,714,342 T→G 8.9% L275V (TTG→GTG)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 14.2% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 12.6% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 10.1% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,801,492 T→G 5.5% pseudogene (1768/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,824,319 T→G 9.1% L213R (CTT→CGT)  CIW80_RS19155 → TolC family protein
RA NZ_CP022686 3,852,459 T→G 8.6% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,895,534 T→G 8.9% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,914,747 A→C 8.8% D132A (GAC→GCC)  yahG → DUF1116 domain‑containing protein
RA NZ_CP022686 3,925,282 A→C 11.4% S154R (AGC→CGC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 15.6% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 11.5% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 8.3% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 8.8% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 8.6% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 6.8% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,949,969 T→G 10.8% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,950,011 T→G 7.0% H127P (CAC→CCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,656 A→C 8.9% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 14.9% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 12.8% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 11.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,248 C→G 10.4% intergenic (+94/‑250) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 10.1% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,025,356 T→G 8.2% N445T (AAC→ACC)  ybaE ← SgrR family transcriptional regulator
RA NZ_CP022686 4,056,941 T→G 9.1% G130G (GGT→GGG adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 20.4% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,079,332 A→C 11.7% W169G (TGG→GGG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,214,457 G→T 9.3% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,287,456 T→G 12.3% noncoding (47/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,290,599 T→G 6.1% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,327,145 G→T 6.4% L189F (TTG→TTT ybhL → Bax inhibitor‑1/YccA family protein
RA NZ_CP022686 4,368,341 T→G 13.8% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,274 A→C 7.3% V244G (GTG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,412,284 A→C 10.5% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,324 C→A 11.5% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,632,186 G→T 5.8% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 7.2% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,642,954 A→C 6.8% Q733P (CAG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,661,469 C→A 8.9% pseudogene (569/655 nt) CIW80_RS26570 → hypothetical protein
RA NZ_CP022686 4,678,041 A→C 17.1% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 21.6% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,700,039 C→A 5.0% P354T (CCA→ACA)  mdoG → glucans biosynthesis protein MdoG
RA NZ_CP022686 4,712,763 A→C 13.5% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,712,985 A→C 5.8% intergenic (‑67/+67) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,764,877 T→G 14.5% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,883 A→T 11.5% intergenic (‑63/‑198) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,783,330 T→G 11.6% E33D (GAA→GAC mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,814,990 T→G 11.2% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,007 T→G 9.7% P76P (CCT→CCG CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,886 T→G 7.8% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,178 T→G 15.2% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,918,999 T→G 7.7% K336N (AAA→AAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,521 A→C 12.4% T39P (ACC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,951,547 T→G 19.2% S47R (AGT→AGG topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 10.5% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,023,952 G→T 6.2% intergenic (+218/‑260) zntB → / → dbpA zinc transporter ZntB/ATP‑dependent RNA helicase DbpA
RA NZ_CP022686 5,043,831 C→T 6.1% D1154D (GAC→GAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,137 G→T 17.7% V1256V (GTG→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3084–2957 340–2896 8275 [7602] [7622] 8246 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4740–4810 5417–5074 265–678 4034 [3796] [3528] 4037 [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 1123563 =154 (0.730)60 (0.280) 42/250 NT 28.0% coding (4434/7950 nt) CIW80_RS05650 phase‑variable autotransporter adhesin UpaE
?NZ_CP022686 1522881 = NA (NA)intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
* ? NZ_CP022686 = 1123565158 (0.750)44 (0.210) 33/250 NT 21.8% coding (4432/7950 nt) CIW80_RS05650 phase‑variable autotransporter adhesin UpaE
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 3743172 =279 (1.330)15 (0.070) 13/248 NT 5.3% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 255 (1.220)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase