breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 182,638 A→C 7.6% V71G (GTC→GGC)  malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 235,765 A→G 5.2% Q260R (CAG→CGG)  pykF → pyruvate kinase PykF
RA NZ_CP022686 355,148 T→G 9.7% G139G (GGT→GGG
D270A (GAC→GCC) 
yeaL →
nimR ←
DUF441 domain‑containing protein
DNA‑binding transcriptional regulator NimR
RA NZ_CP022686 360,227 T→G 22.6% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,290 T→G 12.4% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 377,274 A→C 14.5% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 524,842 T→G 9.1% V203G (GTC→GGC)  yedE → selenium metabolism membrane protein YedE/FdhT
RA NZ_CP022686 556,942 G→T 16.2% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 16.6% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 23.1% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 11.4% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 13.5% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,402 G→T 10.0% intergenic (+162/‑34) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 13.2% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 19.7% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 11.5% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,561 G→T 15.9% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 878,938 T→G 7.7% pseudogene (752/762 nt) yohF ← SDR family oxidoreductase
RA NZ_CP022686 881,733 A→C 16.6% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,046 G→T 9.9% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 12.5% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 12.5% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 14.3% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 6.5% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 15.3% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,098 G→C 7.3% D385H (GAC→CAC)  nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,909 A→C 9.0% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,065,997 A→C 10.1% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 13.4% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 11.6% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,242,419 A→C 6.4% V103G (GTA→GGA)  dapA ← 4‑hydroxy‑tetrahydrodipicolinate synthase
RA NZ_CP022686 1,360,724 G→T 12.2% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 16.6% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,443,501 A→C 8.6% V20G (GTC→GGC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,479,585 T→A 8.0% K3* (AAA→TAA)  ftsB ← cell division protein FtsB
RA NZ_CP022686 1,479,586 A→C 5.3% G2G (GGT→GGG ftsB ← cell division protein FtsB
RA NZ_CP022686 1,479,633 A→C 13.3% intergenic (‑42/+151) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 13.8% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 21.1% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 10.3% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 8.4% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,633,846 T→G 30.3% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 11.1% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 11.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,697,914 T→G 8.8% intergenic (+23/+196) CIW80_RS08360 → / ← tkt prohibitin family protein/transketolase
RA NZ_CP022686 1,705,997 C→A 18.9% intergenic (‑607/‑48) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,032 G→T 16.0% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 10.0% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,828,113 A→C 9.9% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,829,617 A→C 6.3% V93G (GTT→GGT)  gspH ← type II secretion system minor pseudopilin GspH
RA NZ_CP022686 1,911,602 T→G 12.0% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,353 G→T 9.9% G158V (GGT→GTT)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,365 A→C 8.2% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 2,037,521 A→C 10.0% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 17.4% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 15.2% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,053,925 A→C 5.7% V283G (GTT→GGT)  truB ← tRNA pseudouridine(55) synthase TruB
RA NZ_CP022686 2,061,804 A→C 8.3% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 12.9% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 15.6% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.8% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 8.4% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,159,673 C→A 16.9% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 6.7% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,877 C→G 8.7% K35N (AAG→AAC rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 9.0% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,293,025 T→G 5.6% N95T (AAC→ACC)  rtcB ← RNA‑splicing ligase RtcB
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,098 T→G 7.5% F83V (TTT→GTT)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,362,972 T→G 6.4% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,393,050 C→A 7.5% F12L (TTC→TTA dctR → LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 15.7% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,896 A→C 10.6% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,400,789 T→G 7.8% intergenic (+43/‑6) chuY → / → shuU anaerobilin reductase/heme ABC transporter permease ShuU/ChuU
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,421,650 A→C 5.9% T313P (ACG→CCG)  yhjD → inner membrane protein YhjD
RA NZ_CP022686 2,421,691 A→C 10.6% K326N (AAA→AAC yhjD → inner membrane protein YhjD
RA NZ_CP022686 2,460,576 C→A 10.6% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 22.8% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 13.4% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,502,553 A→C 27.0% I539S (ATC→AGC)  selB ← selenocysteine‑specific translation elongation factor
RA NZ_CP022686 2,516,577 A→G 8.6% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,873 A→C 8.5% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 16.0% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,558,864 T→G 6.4% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,705 A→C 8.7% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 8.3% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
RA NZ_CP022686 2,574,451 T→G 9.9% T223P (ACT→CCT)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,603,117 T→G 6.4% G197G (GGT→GGG yicL → carboxylate/amino acid/amine transporter
RA NZ_CP022686 2,676,885 T→G 5.1% D336A (GAC→GCC)  atpD ← F0F1 ATP synthase subunit beta
RA NZ_CP022686 2,681,221 T→G 5.4% T62P (ACC→CCC)  atpF ← F0F1 ATP synthase subunit B
RA NZ_CP022686 2,717,385 T→G 9.2% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,726,623 A→C 5.4% intergenic (‑100/‑31) rhlB ← / → trxA ATP‑dependent RNA helicase RhlB/thioredoxin TrxA
RA NZ_CP022686 2,743,563 T→G 10.5% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,109 A→C 5.5% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 9.9% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 8.0% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,162 G→T 11.9% D52Y (GAT→TAT)  yifL → lipoprotein
RA NZ_CP022686 2,769,166 C→A 14.8% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,186 C→A 16.0% M50I (ATG→ATT rarD ← EamA family transporter RarD
RA NZ_CP022686 2,801,864 T→G 5.6% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,810,040 A→C 7.1% N242T (AAC→ACC)  tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,868,740 T→G 26.5% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 18.0% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,920,428 A→C 8.2% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 3,017,622 A→C 17.0% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 11.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,945 T→G 9.4% F40V (TTT→GTT)  CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,080,461 T→G 12.2% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,136,940 T→G 11.6% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,948 G→T 10.8% L158L (CTC→CTA phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,287,046 A→C 6.5% K117N (AAA→AAC cybC → cytochrome b562
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.5% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,229 T→G 19.3% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,509,248 T→G 31.7% L572R (CTG→CGG)  dnaK → molecular chaperone DnaK
RA NZ_CP022686 3,529,239 A→C 7.3% K397N (AAA→AAC carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,570,234 T→G 9.5% L217V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,604,564 G→T 15.6% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,607,152 T→A 15.9% W622R (TGG→AGG)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,625,683 T→G 10.6% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,667,058 A→C 7.8% D5A (GAC→GCC)  mrcB → bifunctional glycosyl transferase/transpeptidase
RA NZ_CP022686 3,734,367 T→G 23.9% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 18.4% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,736,531 T→G 5.7% N74H (AAC→CAC)  mltD ← murein transglycosylase D
RA NZ_CP022686 3,770,526 C→G 10.9% intergenic (‑262/+201) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 12.5% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,868,846 T→G 16.9% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,289 A→C 12.6% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 12.0% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,935,324 T→G 9.7% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,724 A→C 9.0% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,656 A→C 9.7% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,238 C→G 14.7% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,247 G→T 8.3% intergenic (+93/‑251) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,946 T→G 10.9% V132G (GTT→GGT)  adk → adenylate kinase
RA NZ_CP022686 4,056,951 C→A 17.5% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 17.7% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,188,944 A→C 7.1% V321G (GTA→GGA)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA NZ_CP022686 4,213,260 T→G 7.0% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,268,546 C→T 13.7% A153V (GCA→GTA)  sdhB → succinate dehydrogenase iron‑sulfur subunit SdhB
RA NZ_CP022686 4,269,248 A→C 10.3% intergenic (+443/‑108) sdhB → / → sucA succinate dehydrogenase iron‑sulfur subunit SdhB/2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,280,095 T→G 8.2% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,287,507 T→G 11.1% noncoding (20/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,368,341 T→G 13.4% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,269 A→C 9.3% F246V (TTT→GTT)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,334 A→C 19.1% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,605,579 T→G 14.8% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 11.6% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,186 G→T 8.8% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,638,424 G→T 6.0% L109L (CTG→CTT CIW80_RS23235 → S/F1C fimbrial major subunit operon transcriptional regulator
RA NZ_CP022686 4,642,954 A→C 7.3% Q733P (CAG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,678,047 A→C 22.7% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,704,781 A→C 8.6% G118G (GGT→GGG mdtG ← multidrug efflux MFS transporter MdtG
RA NZ_CP022686 4,764,877 T→G 10.0% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,814,990 T→G 12.1% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,210 T→G 14.9% G112G (GGT→GGG CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,937,070 A→C 7.9% G172G (GGT→GGG trpA ← tryptophan synthase subunit alpha
RA NZ_CP022686 5,041,191 G→A 6.3% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 5.4% E52* (GAA→TAA)  IL2wt no signal →
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 7.2% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,818 A→C 12.8% intergenic (‑183/+391) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,868 Δ1 bp 100% intergenic (‑233/+341) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,883 T→G 7.7% intergenic (‑248/+326) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,899 G→T 7.8% intergenic (‑264/+310) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,915 G→T 10.1% intergenic (‑280/+294) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,922 G→T 8.6% intergenic (‑287/+287) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,933 G→T 8.3% intergenic (‑298/+276) annotated as hok_sok locus ← / ← putative HTH domain protein –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 394–2618 3050–2957 340–2657 7424 [7206] [7195] 7550 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 329060 =128 (0.670)14 (0.070) 14/248 NT 9.9% intergenic (‑8/‑153) ydjA/sppA NAD(P)H nitroreductase/signal peptide peptidase SppA
?NZ_CP022686 = 1524129 NA (NA)intergenic (+7/+233) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 329063128 (0.670)20 (0.100) 16/250 NT 13.5% intergenic (‑11/‑150) ydjA/sppA NAD(P)H nitroreductase/signal peptide peptidase SppA
?NZ_CP022686 1522881 = NA (NA)intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
* ? NZ_CP022686 1123563 =143 (0.750)54 (0.280) 38/250 NT 27.4% coding (4434/7950 nt) CIW80_RS05650 phase‑variable autotransporter adhesin UpaE
?NZ_CP022686 1522881 = NA (NA)intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
* ? NZ_CP022686 = 1123565145 (0.760)35 (0.180) 28/250 NT 19.4% coding (4432/7950 nt) CIW80_RS05650 phase‑variable autotransporter adhesin UpaE
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 1522881 =NA (NA)1919 (0.410) 175/250 NT 20.1% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3885 = 7606 (0.830)coding (277/399 nt) IL2wt no signal
* ? NZ_CP022686 1760449 =NA (NA)2109 (0.450) 174/248 NT 22.0% coding (1100/1101 nt) CIW80_RS08705 IS30‑like element IS30 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3888 7541 (0.820)coding (280/399 nt) IL2wt no signal
* ? NZ_CP022686 = 3395241NA (NA)18 (0.000)
+21 bp
13/208 NT 73.0% pseudogene (11/1107 nt) yjhR helicase YjhR
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 8 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3899666 =207 (1.080)34 (0.200) 28/218 NT 16.5% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 164 (0.980)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681200 (1.040)36 (0.220) 27/218 NT 17.6% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 164 (0.980)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 4514374 =196 (1.020)8 (0.000)
+AGCT
7/242 NT 7.5% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 8 (0.000)coding (1/399 nt) IL2wt no signal
* ? NZ_MW240712 3 =0 (0.000)219 (0.020)
+CC
84/246 NT 100% intergenic (–/+204) –/JNG15_RS00005 –/helix‑turn‑helix domain‑containing protein
?NZ_MW240712 = 3173 0 (0.000)intergenic (+182/–) mobA/– plasmid mobilization protein MobA/–