BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,461,229	T→G	18.7%	noncoding (84/87 nt)	CIW80_RS17340 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,461,229	0	T	G	18.7%	159.0 / 10.2	138	noncoding (84/87 nt)	CIW80_RS17340	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base T (49/60); new base G (9/20); total (58/80)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCC  >  NZ_CP022686/3461091‑3461368

taaGTAAATATAATTGGTGCGTGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                            <  6:577902/139‑1 (MQ=255)

  agaaacgATACTTGGTGCGCGGGGGGGGACTGCCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                            <  3:417148/132‑1 (MQ=18)

   gTAAAGATAATTGGTGCGAGGGGGGGGACTTGCACCCCCACGACCGTAAGAACACTAACACCTGCAGCTAGCGCGTCTACCAATTCAGCCACCTTCGCACAGACATCATTCTTTATTTGATATCGCGTCGGTAGGtcgga                                                                                                                                         >  7:1069305/1‑136 (MQ=17)

       atatatTTGGTGCTTGGGGGGGTACTTCAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCGCGTCTAACAATTCCGCCACCTTCGCACAGTCATCTTACTTTTATTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                     <  8:866081/138‑1 (MQ=18)

        catatgtGGGGCGTGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg                                                                                                                                    <  1:15102/134‑1 (MQ=21)

        aattaTTGGGGCTAGGGGGGGTACTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg                                                                                                                                    <  5:159711/136‑1 (MQ=21)

        aaTAATTGGGGCGCGGGGGGGCACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCATCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                                      <  3:368409/137‑1 (MQ=255)

         aTAATGGGGGCGCGGGGGGGGAATTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg                                                                                                                                   <  7:1077741/140‑1 (MQ=21)

          aaaTTGGGGCGCGGGGGGGCACTAGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggggg                                                                                                                                  <  1:204615/139‑1 (MQ=21)

           aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGTCTGATTACTTTATATGATATGGTCTCGTGTGGGGCTAgggggggg                                                                                                                                 >  5:340271/1‑140 (MQ=14)

             tgggTGCGAGGGGGGGGCCTTGACCCCCCACGTCCGTACGAACACTACCACCTGAAGCTCGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGGGCGAGGGGGGGGAc                                                                                                                               <  6:672209/138‑1 (MQ=21)

               ggggcgcGGGGGGTTCCTTCACCCCCCACGGCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATCTCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                             <  6:25230/137‑1 (MQ=21)

               ggggcgcGGGGGGGCCCTCCACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTAACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                             <  5:992587/137‑1 (MQ=21)

               ggggcccggggggggACATGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                             <  2:767088/133‑1 (MQ=255)

               ggggCGTGGGGGGGGACTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                             <  1:1129065/137‑1 (MQ=255)

               ggggCGAGGGGGGTCCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                             <  6:92222/137‑1 (MQ=255)

               ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTTCTTTATATGATTTGGGGACGTTTGGGGGGAgggggggggg                                                                                                                               >  3:221494/1‑136 (MQ=14)

                   cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCGACCTATTGCGCCACCTTCGCACAGACATCTTGCTTATTTTGATATCTGCTGGTGGGGGGGGCGGGGGGGGaggtggcc                                                                                                                         >  1:235626/1‑133 (MQ=14)

                             gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGTCAGCTTACTTTAATTGATATCGACGCGATGGGTGGGAGGGGTGGGACTTGCACCTCCACGt                                                                                                                 >  4:547611/1‑138 (MQ=17)

                             gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACAGCTTACTTTTATAGAATTCGACCCTGTGGGTGCGAGGCGGGGGGCTTGCACCCCCACGt                                                                                                                 >  4:951923/1‑138 (MQ=17)

                                  acaCCCCCACGGCCGTAACAACACTAACCCATTAAGATAGCCCGTCAACCAATTCCGCCACCTTCGCCCACTCAATTTACTTTTTTTGATTTCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                          <  6:845600/138‑1 (MQ=21)

                                   acccccccCGGCCGTAATAACCCTAACACCTGAAGCTAGTGCGTCTACCAATTCCGCCACCTTCCCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                                                         <  2:656165/138‑1 (MQ=37)

                                    aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGGCAGCTTACTTTTTTTGATTTCGACGCGGGTTGTGGGAGGGGGGGGGCTTGAACCGACACGTCCGtaataa                                                                                                        >  2:219248/1‑140 (MQ=18)

                                        ccccGTCCGTAACAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCCCACAGTCATATTACTTTTTTTTATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                                      <  4:1055720/135‑1 (MQ=37)

                                        ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAACTTTC‑TTTTTTGCTATAGCCTCGTTTGGTGCGAGGTGGGGGGGTTGACCCCCCACGTCCGTAAGaaaaata                                                                                                   >  1:458455/1‑135 (MQ=21)

                                        ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACAGCATACTTTATTTGAATTCGACACGTGTCGGGCGAGGGGGGGGTCTTGCACCCCCACGTCCCTAAGaaaaat                                                                                                    >  1:222330/1‑136 (MQ=18)

                                        ccACGTCCGTAACAACACTAACACCTGAAGCTACCCCGTCTACCAATTCCGCCACCTTCCCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCTAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                    <  8:726295/140‑1 (MQ=37)

                                        ccACGTCCGAAATAACAGTACCACTTGAAGCTTCCTCTTCTAAAAATTCCGCCACATTCCAACAGTCATCATACTTTTTTTGATATCCCGTCTTTTGGGGCGTGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACt                                                                                                    <  2:963575/140‑1 (MQ=16)

                                        ccACGGCCGAAATAACCATAACACCTTAAGCTCGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCTTCTAACTTTTTTTGATATCGCCTGGTTTGGTGCGAGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACt                                                                                                    <  1:699854/140‑1 (MQ=37)

                                         cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCGACTCGGTTGGTGGGAGGGGGGGGCCTTGCACCGCCACGGCCGTAGTAACACtt                                                                                                   >  5:958746/1‑139 (MQ=21)

                                         cACGTCCGAAAGAACACTAACACCGGAAGCTAGCTCGTCTACCAATTCCGCCACCTCCGCACAGTCATCTTACTTTATTTCATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                  <  2:1124489/140‑1 (MQ=37)

                                          ccGTCCGTAATAACCCTAACACCTGAAGCTACCGCGGATCCCAATTCCGCCACCTTCGCACAGTCATGTTACTTTTTTTGATTCCGCCGGTTTTGGTGGGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                  <  2:853977/139‑1 (MQ=21)

                                          agggccGTAACAACACTAACCCCTTAAGCTCCCGCTTCAACAAATTCCGCCCCCTTCCCACAGGCATCTTACTTTTTTTTATATCGCCTCGTTGGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                  <  8:45979/136‑1 (MQ=21)

                                          actgccGAAAGAACAGTAACACCTCATGCTATCGCGTATACCAATACCCCCACCTTCGAACATTCATCTTAATTTTTTTCATATCTCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                  <  6:822910/136‑1 (MQ=21)

                                          aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACAGAATACTTTTATTGATATAGCCGCGATTGGGGCGAGGCGTGGGACTTGAACCACCACGTCCATAAGTactcaaa                                                                                                  >  6:225101/1‑135 (MQ=18)

                                              ccGTTAGATCATTCACACCTGATGCTAGCGCTTATACCTAATCCGCCACCAACCCACAGGCATCTAACTTTTTTTGATAACGCCTCTTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                              <  2:1127515/140‑1 (MQ=18)

                                              ccGTAAGAACACTAACACCTGAAGATCGCGCGTATACAAATTCCGCCACCTTAGCACAGTCATCTAACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                              <  6:1120789/140‑1 (MQ=37)

                                                    gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCATACTTTTTTTGATATCGCAACGTGTCGTGCGAGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                                                          >  3:1165233/1‑138 (MQ=25)

                                                          tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAAATCTTTCTTTATTTGATATCGACTCGTTTGGGGGGAGTGGGGGGACTTGCACCCCATCGTCCGTAATAACACTAACACATGAAGCTaactc                                                                                  >  5:181815/1‑136 (MQ=25)

                                                           aaCACCTGAAGCTCGAGCGTCTACAAATACCGCCACCTTCGCACAGTCATCTTACTTTTTTTTATATCGCCGCGTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                 <  5:968293/140‑1 (MQ=25)

                                                            acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCTCCTCGGTTGGTGCGAGGGGGGGGACTTGATCCACCACCACCCTAACAACACACACCACAGAAGCGAGcgcgg                                                                                >  5:1043468/1‑139 (MQ=25)

                                                                 tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTTTATGATATCGCCTCGTGTGGTGGGAGGGGTGGGCGTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGAGCGTCTa                                                                             >  4:1102020/1‑138 (MQ=25)

                                                                  gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGTCAGCTTACTTTTTTTGATTTCGCCTCGGTTGGTGCGAGGGGGGTGACTTGAACCGCAACGTCCGTAAGAACAAAACCACCTCAAGCTAGAGCCTCTACCa                                                                          >  5:264776/1‑140 (MQ=25)

                                                                    aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTAATTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCACACGTCCTTAAGAACACAAACACCTGAAGCTAGATCGTCTACCa                                                                          >  3:51653/1‑138 (MQ=25)

                                                                        aGCGCGTCTACCAATTCCGCCACCTTCGCAGAGTCTTCTTACTTTTTATGATTTGGGAGCGGTTGGGGGGAGGGGGGGGGCTTGCACCACCTCGTCCATAATAACACTTACACAGATAGCTAGGTCGTGTACAAATTcca                                                                    >  8:108954/1‑139 (MQ=255)

                                                                        aGCGCGTCTACCAATTCCGCCACCTTCGCACACGACGCTTACTTTTATTGAAATGCAAACTGGTGGTGCGAGTCGTGGGAGTTTAACCCCCACGTCATTAAGAACTAATACACCTGTAGCTAGCGCCTCGAAAAATTCCg                                                                    >  8:285129/1‑140 (MQ=255)

                                                                         tctcgTCAACCAATCCCGCCACCTACGCACAGGCAAATAAATATTTTGGATATCCCCTCTTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                     <  3:306578/135‑1 (MQ=17)

                                                                         gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  6:500656/140‑1 (MQ=255)

                                                                         gcgcGTCAACCAATTCCGCCACCTTCGCACAGTCATTATACTTTTTTTGATTTCGCCTCGTTTGGTGCTAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  6:385106/140‑1 (MQ=21)

                                                                          cgcgTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTTTTTGATATCGACGCGGTTGGTGCGAGGGGGGGGACTTGCACCCCCACGTCCATACGAACACAACCACCTGACGCTAGAGCGTCTACCAATTCCGcc                                                                  >  2:1083327/1‑140 (MQ=21)

                                                                             ggataacAATTCCGCCACCTACTCACAGGCATCTAACTTTTTTTGATCTCCCCTACTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                               <  2:1116715/134‑1 (MQ=17)

                                                                                  ccAATTCAGCAATCTTATCACATTCATCTTACTTTTTTTTATATCCCATAGTTTGGTGCTAGGGGGGGTACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                          <  1:597374/140‑1 (MQ=17)

                                                                                   ctaTTCCGCCCCCTACGACCATGCATCTTCCTTTTTTTGATTTCGCCTCGTTTGGGGCGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  6:981284/138‑1 (MQ=17)

                                                                                   cAATTCCGCCACCAACGCACCGGCATCTTAATTTTTTTGATATCGCCCCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  6:335168/140‑1 (MQ=18)

                                                                                   acacaccGCCCCCTCCCCACAGTGATCTTACTTTTATTTATATCGCCTCTTTTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACGCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  8:585981/135‑1 (MQ=17)

                                                                                   aaaatCCGCCACCTACCCACAGTCATCTAACTTTTTTTGATATCCCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                           <  4:170019/134‑1 (MQ=18)

                                                                                   aaaagacGCCACTTACGCAAAGTCATCACACTTTTTTTGATTTCGCCTCGTTTGGTGCTAGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  2:478013/134‑1 (MQ=17)

                                                                                   aaaTTCCGCCACGATACCACAGTCATATTAATTTTTTTGATATCTCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                           <  3:663609/137‑1 (MQ=17)

                                                                                   aaaTTCCGCCACCTTCGAACAGTCATCTTACTATTTATGATCTCGCCTCGTTGGGGGCGTGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  6:636590/139‑1 (MQ=18)

                                                                                   aaaTTCCGCCACCATCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCTAGGGGGGTGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTCGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  2:904903/139‑1 (MQ=21)

                                                                                   aaaTACCGCCACCTTCGCACATGCATCTAACTTTTTTTGAAATCGCCTTTTTTGGGGCGCGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                         <  7:847124/139‑1 (MQ=17)

                                                                                       tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGGTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCATTTCCGCCACCTTCGCCCAgg                                                     >  5:187456/1‑139 (MQ=255)

                                                                                       tCCGCCACCTTCGCACAGTCATCTAACTTTATTTGATATCGCCGCGATTGGTGCGAGGGGGGGGACTTGAACCCCCCCGTCCGTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCAATACCGCCACCTTCGCCCAga                                                     >  5:518366/1‑139 (MQ=18)

                                                                                        ccGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                    <  8:300790/140‑1 (MQ=255)

                                                                                        ccGCCACCTCCCCAACGCCATCATACTTTTTTTTATATCCCGTCGTTGGGGGCGTGGGGGGGGACTTTACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                    <  5:1110436/140‑1 (MQ=17)

                                                                                        ccGCAACCTTCGCACAGCCACCTAACTTTTTTTCAAAGCCCGCCGTTTGGTGCGTGGGGGGGGACTTCACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                    <  5:306633/140‑1 (MQ=14)

                                                                                          gCCACCTTCGCACAGTCATCTTAAGTTTTTTGATCTCTCCGGGTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                    <  4:452375/138‑1 (MQ=17)

                                                                                           ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACAATAACACCTGAAGCTAGCGCGTCTAACAATTCCGCAACCTTCCCCAACTGCGa                                                 >  7:683526/1‑140 (MQ=21)

                                                                                            cACCTTCGCACATTCATCATACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                <  6:870975/140‑1 (MQ=255)

                                                                                            cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                             >  4:1044501/1‑111 (MQ=255)

                                                                                            cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                             <  3:1044501/111‑1 (MQ=255)

                                                                                            cACCTTCGAACTTTCATCTTACTTTTTTTGATATCGCGTGTTTTGGTGCGAGGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                  <  3:560204/138‑1 (MQ=17)

                                                                                            cACCTTCGAACAGGCATCTTACTTTTTTTGATCTCGCCTCGTTGGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                <  8:871489/140‑1 (MQ=255)

                                                                                            cACCTTCCCACAGTCATCTTACTTTTTTTGCATGCCCCTCGTTTGGTGCGAGGGGGGTGAGTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                <  1:733684/140‑1 (MQ=18)

                                                                                            cACCTACTCACAGGCCTCTTACTTTTTTTGATATCGCCTCTTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                  <  4:614183/138‑1 (MQ=18)

                                                                                             aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGa                                                 >  4:1128097/1‑138 (MQ=255)

                                                                                              ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGAGCGTCTACCAATTCCGCCACCTTCGCACAGTGcaataa                                              >  1:726494/1‑135 (MQ=255)

                                                                                                      aactCAACTAACTTTTATTGAATTACCCTCTATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                      <  2:726695/137‑1 (MQ=14)

                                                                                                        ttcataatACATTTTTTGATATCGCCTCGTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGATCAATATCAACGt                                    <  6:368045/133‑1 (MQ=18)

                                                                                                         tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGCAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGAGGAGCAATATCAACGTg                                   >  2:97686/1‑140 (MQ=255)

                                                                                                         tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGAGATCAATATAAACgag                                   >  8:655405/1‑138 (MQ=18)

                                                                                                         tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGAAAGAACACAAACACCTGAAGCTATCGCGTCTACCAATTACGCCACCTTCGCCCAGTGCGAGCAAAAACAACGTg                                   >  1:447409/1‑140 (MQ=21)

                                                                                                           aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATTACAACGTg                                   >  4:829662/1‑138 (MQ=255)

                                                                                                           aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACCTCCGTAAGAACACAAACACCATAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                   >  3:407823/1‑138 (MQ=255)

                                                                                                             cTTACATTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATAACAAAGTGGttt                               >  7:1060722/1‑140 (MQ=18)

                                                                                                              ttAATTTTTATTTTATCGCCTGGTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                              <  1:757144/140‑1 (MQ=18)

                                                                                                                aCTTTTTTTGATATCGCCTCGTTTGGTGCGTGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGGCTACCAAATCCGCCACCTTCGCCAGCTGCGAGCAAAATCCTCGTGGtttt                              >  4:379001/1‑138 (MQ=18)

                                                                                                                  tttttttGATATCGCTTCTTTGGGTGCGGGGGGGGGGAGTTTACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                          <  5:40792/140‑1 (MQ=21)

                                                                                                                   ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTTCCAGCAATATCAACGAGGTTTTAGGTg                         >  8:649283/1‑140 (MQ=18)

                                                                                                                   ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  8:1056232/98‑1 (MQ=255)

                                                                                                                   ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTGGTTTTTGCTg                         >  8:34356/1‑140 (MQ=255)

                                                                                                                   ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATAAACGTGTTTTTTGGTg                         >  7:309846/1‑140 (MQ=255)

                                                                                                                   ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACAGCGAGCAATATCAACGTGGTTTTTGGTg                         >  6:12915/1‑140 (MQ=255)

                                                                                                                   ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAACTGAAGCTAGCGCGTCAACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTTGtt                         >  1:808768/1‑139 (MQ=255)

                                                                                                                   ttttctTATATCCCCCCGTTTGGGGGGTGGGGGGGCACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                           <  3:364419/138‑1 (MQ=18)

                                                                                                                     ttttGATATCGCCTCGTTTTGTGCTGGGGGGGGGACTACACCCCCCACGTCCGTAATAACACTACCCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACATTCGCCCAGTGCGAGCAATTTCAACGTGGCTTTTGGTg                         <  4:407823/138‑1 (MQ=18)

                                                                                                                                ccTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGaa            >  2:1032785/1‑139 (MQ=255)

                                                                                                                                   cGTTTGGGGGGTGGGGTGGCACTTGAACCCCCTCGTCCTTAAGAACACTAACACCTGAAGCTAGCTGGTATACCAATTCCGACACCTTCGCCCATTCCTAGAAATCACAACGTGTTTTTTGGTGCGAGGGGGGGGACTTg         <  6:589434/140‑1 (MQ=12)

                                                                                                                                    gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCAACCTTCGCCAAGGGCGAGAAATATAAACTAGGTTTTTGTTGCGAGGGGGGGGACTTGa        >  7:90800/1‑140 (MQ=17)

                                                                                                                                    gTTTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATAAAAGTGGTTTTTGGTTCGAGGGGTGGGACTTGa        >  7:90804/1‑140 (MQ=18)

GAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCC  >  NZ_CP022686/3461091‑3461368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`